Arthrobacter phage Mufasa8

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Mufasoctovirus; Arthrobacter virus Mufasa8

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 104 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A649VM95|A0A649VM95_9CAUD Uncharacterized protein OS=Arthrobacter phage Mufasa8 OX=2656526 GN=103 PE=4 SV=1
MM1 pKa = 7.25TATTEE6 pKa = 3.98APTTNLHH13 pKa = 5.97QPVEE17 pKa = 4.21RR18 pKa = 11.84DD19 pKa = 3.71GNHH22 pKa = 6.94LDD24 pKa = 5.32CGAPDD29 pKa = 3.83CGYY32 pKa = 11.83SMFTHH37 pKa = 7.51PIATPTFKK45 pKa = 10.85DD46 pKa = 3.54QPEE49 pKa = 3.99KK50 pKa = 10.38MATYY54 pKa = 9.6WRR56 pKa = 11.84DD57 pKa = 2.78QFQRR61 pKa = 11.84HH62 pKa = 6.0HH63 pKa = 7.09SDD65 pKa = 2.48QYY67 pKa = 11.41APGRR71 pKa = 11.84APDD74 pKa = 3.55IAVDD78 pKa = 3.74WEE80 pKa = 4.42VSACCSICEE89 pKa = 4.33DD90 pKa = 4.03KK91 pKa = 11.11IGDD94 pKa = 3.76IVVVDD99 pKa = 4.92SEE101 pKa = 4.6TLTCKK106 pKa = 10.74DD107 pKa = 5.02CGTTWDD113 pKa = 3.41MHH115 pKa = 5.26GTSGEE120 pKa = 3.99LAEE123 pKa = 4.42QEE125 pKa = 3.89EE126 pKa = 4.77DD127 pKa = 3.25

Molecular weight: 14.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A649VMF4|A0A649VMF4_9CAUD Uncharacterized protein OS=Arthrobacter phage Mufasa8 OX=2656526 GN=80 PE=4 SV=1
MM1 pKa = 7.24EE2 pKa = 6.13RR3 pKa = 11.84GRR5 pKa = 11.84GRR7 pKa = 11.84PKK9 pKa = 10.46ADD11 pKa = 3.09QEE13 pKa = 4.27EE14 pKa = 4.63FLQLHH19 pKa = 5.74HH20 pKa = 6.77QGLSASQIAEE30 pKa = 3.96RR31 pKa = 11.84MGCTARR37 pKa = 11.84TIVRR41 pKa = 11.84LRR43 pKa = 11.84ARR45 pKa = 11.84TGVAAPAPTGKK56 pKa = 10.25RR57 pKa = 11.84VDD59 pKa = 4.21AEE61 pKa = 3.62WHH63 pKa = 5.52AKK65 pKa = 8.72VRR67 pKa = 11.84EE68 pKa = 4.02LHH70 pKa = 7.15AEE72 pKa = 4.12GLSQTDD78 pKa = 3.31IARR81 pKa = 11.84LTGVTPQTVARR92 pKa = 11.84HH93 pKa = 5.75HH94 pKa = 7.49PDD96 pKa = 3.29TGWDD100 pKa = 3.16RR101 pKa = 11.84PTIGRR106 pKa = 11.84YY107 pKa = 8.72GRR109 pKa = 11.84AVKK112 pKa = 9.1TLNSLPSHH120 pKa = 6.61LEE122 pKa = 3.48AVQIGASTT130 pKa = 3.32

Molecular weight: 14.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

104

0

104

19005

37

1110

182.7

19.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.444 ± 0.586

0.768 ± 0.105

5.777 ± 0.227

5.983 ± 0.333

2.526 ± 0.148

7.924 ± 0.387

2.405 ± 0.201

4.157 ± 0.139

4.446 ± 0.264

7.682 ± 0.279

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.378 ± 0.16

3.247 ± 0.19

6.409 ± 0.525

3.678 ± 0.134

5.572 ± 0.364

5.483 ± 0.339

7.756 ± 0.282

7.209 ± 0.212

1.636 ± 0.122

2.52 ± 0.189
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski