Amino acid dipepetide frequency for Arthrobacter phage Mufasa8

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
19.575AlaAla: 19.575 ± 1.709
0.737AlaCys: 0.737 ± 0.198
6.946AlaAsp: 6.946 ± 0.716
9.472AlaGlu: 9.472 ± 1.11
2.631AlaPhe: 2.631 ± 0.279
9.051AlaGly: 9.051 ± 1.031
2.947AlaHis: 2.947 ± 0.521
4.683AlaIle: 4.683 ± 0.574
6.314AlaLys: 6.314 ± 0.759
8.209AlaLeu: 8.209 ± 0.598
3.631AlaMet: 3.631 ± 0.594
3.736AlaAsn: 3.736 ± 0.513
9.524AlaPro: 9.524 ± 1.904
4.262AlaGln: 4.262 ± 0.478
6.525AlaArg: 6.525 ± 0.644
5.683AlaSer: 5.683 ± 0.657
8.156AlaThr: 8.156 ± 0.743
8.367AlaVal: 8.367 ± 0.575
1.842AlaTrp: 1.842 ± 0.389
2.157AlaTyr: 2.157 ± 0.272
0.0AlaXaa: 0.0 ± 0.0
Cys
0.947CysAla: 0.947 ± 0.234
0.053CysCys: 0.053 ± 0.055
0.421CysAsp: 0.421 ± 0.213
0.421CysGlu: 0.421 ± 0.136
0.158CysPhe: 0.158 ± 0.083
1.052CysGly: 1.052 ± 0.286
0.105CysHis: 0.105 ± 0.078
0.158CysIle: 0.158 ± 0.083
0.368CysLys: 0.368 ± 0.126
0.316CysLeu: 0.316 ± 0.123
0.105CysMet: 0.105 ± 0.07
0.105CysAsn: 0.105 ± 0.074
0.895CysPro: 0.895 ± 0.26
0.316CysGln: 0.316 ± 0.157
0.421CysArg: 0.421 ± 0.136
0.474CysSer: 0.474 ± 0.183
0.631CysThr: 0.631 ± 0.176
0.368CysVal: 0.368 ± 0.156
0.053CysTrp: 0.053 ± 0.05
0.316CysTyr: 0.316 ± 0.125
0.0CysXaa: 0.0 ± 0.0
Asp
6.788AspAla: 6.788 ± 0.623
0.526AspCys: 0.526 ± 0.216
3.578AspAsp: 3.578 ± 0.466
3.578AspGlu: 3.578 ± 0.442
1.263AspPhe: 1.263 ± 0.225
5.893AspGly: 5.893 ± 0.564
1.526AspHis: 1.526 ± 0.316
2.0AspIle: 2.0 ± 0.386
2.0AspLys: 2.0 ± 0.325
4.841AspLeu: 4.841 ± 0.563
1.894AspMet: 1.894 ± 0.301
1.105AspAsn: 1.105 ± 0.24
3.683AspPro: 3.683 ± 0.401
1.579AspGln: 1.579 ± 0.262
2.789AspArg: 2.789 ± 0.442
3.368AspSer: 3.368 ± 0.367
3.315AspThr: 3.315 ± 0.364
4.736AspVal: 4.736 ± 0.46
1.579AspTrp: 1.579 ± 0.35
1.736AspTyr: 1.736 ± 0.274
0.0AspXaa: 0.0 ± 0.0
Glu
9.63GluAla: 9.63 ± 1.063
0.158GluCys: 0.158 ± 0.103
3.21GluAsp: 3.21 ± 0.412
3.894GluGlu: 3.894 ± 0.617
1.684GluPhe: 1.684 ± 0.255
4.052GluGly: 4.052 ± 0.483
0.737GluHis: 0.737 ± 0.184
2.105GluIle: 2.105 ± 0.348
2.368GluLys: 2.368 ± 0.383
5.63GluLeu: 5.63 ± 0.615
1.0GluMet: 1.0 ± 0.275
1.631GluAsn: 1.631 ± 0.276
4.157GluPro: 4.157 ± 0.619
2.105GluGln: 2.105 ± 0.346
3.526GluArg: 3.526 ± 0.632
2.947GluSer: 2.947 ± 0.401
3.368GluThr: 3.368 ± 0.466
4.157GluVal: 4.157 ± 0.45
1.526GluTrp: 1.526 ± 0.294
1.947GluTyr: 1.947 ± 0.313
0.0GluXaa: 0.0 ± 0.0
Phe
2.473PheAla: 2.473 ± 0.359
0.105PheCys: 0.105 ± 0.072
1.684PheAsp: 1.684 ± 0.301
1.105PheGlu: 1.105 ± 0.233
0.579PhePhe: 0.579 ± 0.182
1.579PheGly: 1.579 ± 0.291
0.631PheHis: 0.631 ± 0.183
0.895PheIle: 0.895 ± 0.212
1.579PheLys: 1.579 ± 0.244
2.0PheLeu: 2.0 ± 0.284
0.631PheMet: 0.631 ± 0.154
1.158PheAsn: 1.158 ± 0.254
1.21PhePro: 1.21 ± 0.266
1.263PheGln: 1.263 ± 0.279
1.421PheArg: 1.421 ± 0.262
1.421PheSer: 1.421 ± 0.345
2.315PheThr: 2.315 ± 0.347
2.105PheVal: 2.105 ± 0.342
0.21PheTrp: 0.21 ± 0.109
0.895PheTyr: 0.895 ± 0.209
0.0PheXaa: 0.0 ± 0.0
Gly
7.998GlyAla: 7.998 ± 0.747
0.684GlyCys: 0.684 ± 0.213
3.947GlyAsp: 3.947 ± 0.495
4.104GlyGlu: 4.104 ± 0.421
2.315GlyPhe: 2.315 ± 0.385
7.209GlyGly: 7.209 ± 1.199
1.947GlyHis: 1.947 ± 0.31
3.21GlyIle: 3.21 ± 0.484
4.21GlyLys: 4.21 ± 0.445
6.157GlyLeu: 6.157 ± 0.455
2.0GlyMet: 2.0 ± 0.421
2.789GlyAsn: 2.789 ± 0.423
3.052GlyPro: 3.052 ± 0.426
3.105GlyGln: 3.105 ± 0.459
4.262GlyArg: 4.262 ± 0.478
4.42GlySer: 4.42 ± 0.672
7.419GlyThr: 7.419 ± 0.619
6.051GlyVal: 6.051 ± 0.531
1.894GlyTrp: 1.894 ± 0.283
2.473GlyTyr: 2.473 ± 0.368
0.0GlyXaa: 0.0 ± 0.0
His
2.526HisAla: 2.526 ± 0.415
0.105HisCys: 0.105 ± 0.084
1.263HisAsp: 1.263 ± 0.294
1.421HisGlu: 1.421 ± 0.299
0.526HisPhe: 0.526 ± 0.161
2.263HisGly: 2.263 ± 0.325
1.316HisHis: 1.316 ± 0.382
0.842HisIle: 0.842 ± 0.225
0.895HisLys: 0.895 ± 0.199
2.315HisLeu: 2.315 ± 0.332
0.368HisMet: 0.368 ± 0.158
1.0HisAsn: 1.0 ± 0.222
1.789HisPro: 1.789 ± 0.507
0.947HisGln: 0.947 ± 0.201
1.736HisArg: 1.736 ± 0.409
0.947HisSer: 0.947 ± 0.207
1.105HisThr: 1.105 ± 0.206
1.789HisVal: 1.789 ± 0.301
0.263HisTrp: 0.263 ± 0.109
0.631HisTyr: 0.631 ± 0.188
0.0HisXaa: 0.0 ± 0.0
Ile
5.841IleAla: 5.841 ± 0.461
0.368IleCys: 0.368 ± 0.132
2.999IleAsp: 2.999 ± 0.313
2.736IleGlu: 2.736 ± 0.359
0.895IlePhe: 0.895 ± 0.148
2.578IleGly: 2.578 ± 0.309
0.842IleHis: 0.842 ± 0.185
1.421IleIle: 1.421 ± 0.352
2.21IleLys: 2.21 ± 0.348
2.421IleLeu: 2.421 ± 0.361
0.947IleMet: 0.947 ± 0.218
1.684IleAsn: 1.684 ± 0.282
2.105IlePro: 2.105 ± 0.336
1.526IleGln: 1.526 ± 0.265
2.21IleArg: 2.21 ± 0.289
1.894IleSer: 1.894 ± 0.338
4.052IleThr: 4.052 ± 0.554
2.947IleVal: 2.947 ± 0.363
0.526IleTrp: 0.526 ± 0.166
1.368IleTyr: 1.368 ± 0.257
0.0IleXaa: 0.0 ± 0.0
Lys
6.525LysAla: 6.525 ± 0.647
0.21LysCys: 0.21 ± 0.1
2.473LysAsp: 2.473 ± 0.403
2.21LysGlu: 2.21 ± 0.306
0.842LysPhe: 0.842 ± 0.191
3.157LysGly: 3.157 ± 0.434
0.947LysHis: 0.947 ± 0.184
1.736LysIle: 1.736 ± 0.279
2.631LysLys: 2.631 ± 0.588
4.21LysLeu: 4.21 ± 0.525
1.421LysMet: 1.421 ± 0.295
0.947LysAsn: 0.947 ± 0.212
3.368LysPro: 3.368 ± 0.65
1.947LysGln: 1.947 ± 0.301
2.631LysArg: 2.631 ± 0.315
2.894LysSer: 2.894 ± 0.483
2.473LysThr: 2.473 ± 0.381
2.736LysVal: 2.736 ± 0.371
0.368LysTrp: 0.368 ± 0.157
0.737LysTyr: 0.737 ± 0.212
0.0LysXaa: 0.0 ± 0.0
Leu
8.788LeuAla: 8.788 ± 0.827
0.579LeuCys: 0.579 ± 0.205
5.578LeuAsp: 5.578 ± 0.583
3.262LeuGlu: 3.262 ± 0.475
1.473LeuPhe: 1.473 ± 0.235
5.683LeuGly: 5.683 ± 0.524
2.157LeuHis: 2.157 ± 0.36
2.894LeuIle: 2.894 ± 0.428
2.789LeuLys: 2.789 ± 0.375
6.472LeuLeu: 6.472 ± 0.75
1.526LeuMet: 1.526 ± 0.261
2.894LeuAsn: 2.894 ± 0.366
4.578LeuPro: 4.578 ± 0.694
3.105LeuGln: 3.105 ± 0.333
4.946LeuArg: 4.946 ± 0.704
5.209LeuSer: 5.209 ± 0.545
7.63LeuThr: 7.63 ± 0.535
4.683LeuVal: 4.683 ± 0.472
0.895LeuTrp: 0.895 ± 0.244
1.684LeuTyr: 1.684 ± 0.324
0.0LeuXaa: 0.0 ± 0.0
Met
4.368MetAla: 4.368 ± 0.397
0.21MetCys: 0.21 ± 0.092
1.158MetAsp: 1.158 ± 0.228
1.368MetGlu: 1.368 ± 0.286
0.684MetPhe: 0.684 ± 0.225
2.105MetGly: 2.105 ± 0.481
0.579MetHis: 0.579 ± 0.16
0.684MetIle: 0.684 ± 0.16
0.737MetLys: 0.737 ± 0.207
1.473MetLeu: 1.473 ± 0.269
0.316MetMet: 0.316 ± 0.168
1.052MetAsn: 1.052 ± 0.212
1.263MetPro: 1.263 ± 0.217
0.737MetGln: 0.737 ± 0.212
0.789MetArg: 0.789 ± 0.172
1.579MetSer: 1.579 ± 0.286
2.263MetThr: 2.263 ± 0.308
1.631MetVal: 1.631 ± 0.286
0.368MetTrp: 0.368 ± 0.123
0.421MetTyr: 0.421 ± 0.151
0.0MetXaa: 0.0 ± 0.0
Asn
3.526AsnAla: 3.526 ± 0.429
0.368AsnCys: 0.368 ± 0.148
1.894AsnAsp: 1.894 ± 0.363
2.105AsnGlu: 2.105 ± 0.372
0.895AsnPhe: 0.895 ± 0.186
3.736AsnGly: 3.736 ± 0.397
0.737AsnHis: 0.737 ± 0.179
1.736AsnIle: 1.736 ± 0.307
1.052AsnLys: 1.052 ± 0.229
2.421AsnLeu: 2.421 ± 0.414
0.579AsnMet: 0.579 ± 0.15
0.737AsnAsn: 0.737 ± 0.235
2.368AsnPro: 2.368 ± 0.368
1.579AsnGln: 1.579 ± 0.281
1.947AsnArg: 1.947 ± 0.358
1.684AsnSer: 1.684 ± 0.279
2.578AsnThr: 2.578 ± 0.373
1.579AsnVal: 1.579 ± 0.255
0.21AsnTrp: 0.21 ± 0.111
0.737AsnTyr: 0.737 ± 0.177
0.0AsnXaa: 0.0 ± 0.0
Pro
9.945ProAla: 9.945 ± 1.898
0.526ProCys: 0.526 ± 0.177
3.947ProAsp: 3.947 ± 0.434
4.21ProGlu: 4.21 ± 0.436
1.789ProPhe: 1.789 ± 0.277
4.736ProGly: 4.736 ± 0.482
1.684ProHis: 1.684 ± 0.248
2.315ProIle: 2.315 ± 0.342
2.263ProLys: 2.263 ± 0.359
3.157ProLeu: 3.157 ± 0.43
1.21ProMet: 1.21 ± 0.261
1.789ProAsn: 1.789 ± 0.371
4.368ProPro: 4.368 ± 0.867
2.0ProGln: 2.0 ± 0.298
3.105ProArg: 3.105 ± 0.658
3.315ProSer: 3.315 ± 0.458
5.578ProThr: 5.578 ± 0.537
6.314ProVal: 6.314 ± 0.949
0.895ProTrp: 0.895 ± 0.185
0.947ProTyr: 0.947 ± 0.213
0.0ProXaa: 0.0 ± 0.0
Gln
4.315GlnAla: 4.315 ± 0.408
0.368GlnCys: 0.368 ± 0.153
1.158GlnAsp: 1.158 ± 0.274
2.052GlnGlu: 2.052 ± 0.334
1.21GlnPhe: 1.21 ± 0.238
2.21GlnGly: 2.21 ± 0.357
1.052GlnHis: 1.052 ± 0.225
1.894GlnIle: 1.894 ± 0.324
1.579GlnLys: 1.579 ± 0.354
4.315GlnLeu: 4.315 ± 0.44
0.842GlnMet: 0.842 ± 0.18
1.473GlnAsn: 1.473 ± 0.326
2.947GlnPro: 2.947 ± 0.534
1.947GlnGln: 1.947 ± 0.389
1.736GlnArg: 1.736 ± 0.303
2.157GlnSer: 2.157 ± 0.321
2.315GlnThr: 2.315 ± 0.338
1.526GlnVal: 1.526 ± 0.366
0.842GlnTrp: 0.842 ± 0.292
0.842GlnTyr: 0.842 ± 0.211
0.0GlnXaa: 0.0 ± 0.0
Arg
5.63ArgAla: 5.63 ± 0.652
0.737ArgCys: 0.737 ± 0.215
3.473ArgAsp: 3.473 ± 0.513
3.262ArgGlu: 3.262 ± 0.41
1.842ArgPhe: 1.842 ± 0.335
3.841ArgGly: 3.841 ± 0.512
1.631ArgHis: 1.631 ± 0.377
2.684ArgIle: 2.684 ± 0.363
3.262ArgLys: 3.262 ± 0.714
4.262ArgLeu: 4.262 ± 0.62
1.052ArgMet: 1.052 ± 0.217
2.052ArgAsn: 2.052 ± 0.358
3.683ArgPro: 3.683 ± 0.515
2.0ArgGln: 2.0 ± 0.361
3.683ArgArg: 3.683 ± 0.614
2.526ArgSer: 2.526 ± 0.343
3.105ArgThr: 3.105 ± 0.447
4.788ArgVal: 4.788 ± 0.456
0.737ArgTrp: 0.737 ± 0.178
1.473ArgTyr: 1.473 ± 0.258
0.0ArgXaa: 0.0 ± 0.0
Ser
5.841SerAla: 5.841 ± 0.568
0.368SerCys: 0.368 ± 0.13
2.315SerAsp: 2.315 ± 0.333
2.21SerGlu: 2.21 ± 0.365
1.789SerPhe: 1.789 ± 0.34
5.367SerGly: 5.367 ± 0.834
1.105SerHis: 1.105 ± 0.251
2.736SerIle: 2.736 ± 0.344
2.105SerLys: 2.105 ± 0.331
4.157SerLeu: 4.157 ± 0.434
1.789SerMet: 1.789 ± 0.281
1.316SerAsn: 1.316 ± 0.235
3.683SerPro: 3.683 ± 0.441
1.579SerGln: 1.579 ± 0.289
3.157SerArg: 3.157 ± 0.435
3.42SerSer: 3.42 ± 0.556
4.736SerThr: 4.736 ± 0.468
4.157SerVal: 4.157 ± 0.648
1.052SerTrp: 1.052 ± 0.244
1.894SerTyr: 1.894 ± 0.32
0.0SerXaa: 0.0 ± 0.0
Thr
7.735ThrAla: 7.735 ± 0.542
0.684ThrCys: 0.684 ± 0.245
3.999ThrAsp: 3.999 ± 0.576
4.736ThrGlu: 4.736 ± 0.492
2.21ThrPhe: 2.21 ± 0.405
6.893ThrGly: 6.893 ± 0.752
2.052ThrHis: 2.052 ± 0.541
3.999ThrIle: 3.999 ± 0.523
3.262ThrLys: 3.262 ± 0.61
5.893ThrLeu: 5.893 ± 0.599
1.789ThrMet: 1.789 ± 0.285
3.21ThrAsn: 3.21 ± 0.474
5.578ThrPro: 5.578 ± 0.577
2.789ThrGln: 2.789 ± 0.324
3.789ThrArg: 3.789 ± 0.523
3.578ThrSer: 3.578 ± 0.393
6.314ThrThr: 6.314 ± 0.611
5.683ThrVal: 5.683 ± 0.623
1.105ThrTrp: 1.105 ± 0.23
2.263ThrTyr: 2.263 ± 0.345
0.0ThrXaa: 0.0 ± 0.0
Val
7.577ValAla: 7.577 ± 0.769
0.526ValCys: 0.526 ± 0.149
5.209ValAsp: 5.209 ± 0.474
5.788ValGlu: 5.788 ± 0.573
1.158ValPhe: 1.158 ± 0.226
4.368ValGly: 4.368 ± 0.597
1.158ValHis: 1.158 ± 0.249
3.473ValIle: 3.473 ± 0.455
3.368ValLys: 3.368 ± 0.426
4.841ValLeu: 4.841 ± 0.396
1.526ValMet: 1.526 ± 0.269
2.315ValAsn: 2.315 ± 0.328
3.999ValPro: 3.999 ± 0.483
2.421ValGln: 2.421 ± 0.364
4.368ValArg: 4.368 ± 0.524
4.683ValSer: 4.683 ± 0.553
6.472ValThr: 6.472 ± 0.553
5.736ValVal: 5.736 ± 0.725
1.052ValTrp: 1.052 ± 0.304
2.052ValTyr: 2.052 ± 0.502
0.0ValXaa: 0.0 ± 0.0
Trp
2.052TrpAla: 2.052 ± 0.342
0.158TrpCys: 0.158 ± 0.083
1.158TrpAsp: 1.158 ± 0.248
0.842TrpGlu: 0.842 ± 0.211
0.526TrpPhe: 0.526 ± 0.215
1.263TrpGly: 1.263 ± 0.267
0.684TrpHis: 0.684 ± 0.197
0.684TrpIle: 0.684 ± 0.177
0.737TrpLys: 0.737 ± 0.187
1.526TrpLeu: 1.526 ± 0.269
0.579TrpMet: 0.579 ± 0.176
0.579TrpAsn: 0.579 ± 0.165
0.526TrpPro: 0.526 ± 0.136
0.474TrpGln: 0.474 ± 0.168
0.947TrpArg: 0.947 ± 0.241
0.895TrpSer: 0.895 ± 0.221
1.105TrpThr: 1.105 ± 0.19
1.052TrpVal: 1.052 ± 0.245
0.316TrpTrp: 0.316 ± 0.138
0.263TrpTyr: 0.263 ± 0.115
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.368TyrAla: 2.368 ± 0.305
0.21TyrCys: 0.21 ± 0.113
1.368TyrAsp: 1.368 ± 0.262
1.052TyrGlu: 1.052 ± 0.24
0.789TyrPhe: 0.789 ± 0.209
2.21TyrGly: 2.21 ± 0.443
0.21TyrHis: 0.21 ± 0.096
1.421TyrIle: 1.421 ± 0.293
0.737TyrLys: 0.737 ± 0.203
2.21TyrLeu: 2.21 ± 0.378
0.526TyrMet: 0.526 ± 0.16
0.895TyrAsn: 0.895 ± 0.247
1.316TyrPro: 1.316 ± 0.302
1.105TyrGln: 1.105 ± 0.286
1.736TyrArg: 1.736 ± 0.279
1.736TyrSer: 1.736 ± 0.362
2.631TyrThr: 2.631 ± 0.584
1.684TyrVal: 1.684 ± 0.378
0.631TyrTrp: 0.631 ± 0.189
0.368TyrTyr: 0.368 ± 0.13
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 104 proteins (19005 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski