Phyllobacterium sp. YR531

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Phyllobacteriaceae; Phyllobacterium; unclassified Phyllobacterium

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4844 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|J3C622|J3C622_9RHIZ Peptidase T OS=Phyllobacterium sp. YR531 OX=1144343 GN=PMI41_04132 PE=3 SV=1
MM1 pKa = 7.28VLTDD5 pKa = 4.18GDD7 pKa = 3.97DD8 pKa = 3.85VYY10 pKa = 11.39TGTAGNDD17 pKa = 3.04LVYY20 pKa = 11.05ALNGNDD26 pKa = 3.71KK27 pKa = 11.0VSGGLGNDD35 pKa = 3.17QFYY38 pKa = 11.19GGAGSDD44 pKa = 3.53ILDD47 pKa = 4.36GGDD50 pKa = 4.16GDD52 pKa = 4.77DD53 pKa = 5.33LIYY56 pKa = 10.53GYY58 pKa = 10.73GSSGTDD64 pKa = 3.12GGPDD68 pKa = 3.28VPGNDD73 pKa = 3.36TLLGGRR79 pKa = 11.84GEE81 pKa = 4.23DD82 pKa = 3.33QLYY85 pKa = 11.04SGIGNDD91 pKa = 4.19FLDD94 pKa = 4.62GGADD98 pKa = 3.67DD99 pKa = 6.04DD100 pKa = 5.25YY101 pKa = 11.56ISSGDD106 pKa = 3.54GNDD109 pKa = 3.4RR110 pKa = 11.84LFGSDD115 pKa = 5.47GDD117 pKa = 3.94DD118 pKa = 3.59TLNGGAGNDD127 pKa = 3.43ILNGGTGEE135 pKa = 4.14DD136 pKa = 4.09RR137 pKa = 11.84MTGGTGSDD145 pKa = 2.75IYY147 pKa = 11.44YY148 pKa = 10.29VDD150 pKa = 3.83SADD153 pKa = 4.32DD154 pKa = 3.72EE155 pKa = 4.91VNEE158 pKa = 4.25FSGGGIDD165 pKa = 4.71RR166 pKa = 11.84VISSISFSLTSDD178 pKa = 3.52SFVVSGDD185 pKa = 3.48VEE187 pKa = 4.45YY188 pKa = 9.64LTLSGSANIDD198 pKa = 3.36GQGNSLNNLIYY209 pKa = 10.85GNNGNNALRR218 pKa = 11.84GWGGHH223 pKa = 4.46DD224 pKa = 4.65TIYY227 pKa = 10.87GWAGNDD233 pKa = 3.09ILDD236 pKa = 4.03GGFGKK241 pKa = 10.49DD242 pKa = 3.11MLVGGEE248 pKa = 4.41GNDD251 pKa = 3.36TYY253 pKa = 11.6YY254 pKa = 11.08VDD256 pKa = 3.57SAGDD260 pKa = 3.4RR261 pKa = 11.84VVEE264 pKa = 4.06ARR266 pKa = 11.84VTGIDD271 pKa = 3.35TVIASVSFSTVGQYY285 pKa = 9.83IEE287 pKa = 4.99NVTLTGSANINATGNSLGNKK307 pKa = 9.22LVGNSGNNSLDD318 pKa = 3.25GKK320 pKa = 9.1TGADD324 pKa = 3.54SMSGGAGSDD333 pKa = 2.97KK334 pKa = 11.12YY335 pKa = 11.47YY336 pKa = 10.32IDD338 pKa = 3.58NAGDD342 pKa = 3.85KK343 pKa = 10.73VIEE346 pKa = 4.38GNVAGTDD353 pKa = 3.68TVSSSVSFATGGQHH367 pKa = 6.58IEE369 pKa = 4.15NVLLTGSASINATGNALNNYY389 pKa = 9.48LVGNSGANIISGGAGNDD406 pKa = 3.72LLTGGAGRR414 pKa = 11.84DD415 pKa = 2.86AFVFNTAPASNNRR428 pKa = 11.84DD429 pKa = 3.48TITDD433 pKa = 3.97FNVVADD439 pKa = 5.04TIWMDD444 pKa = 3.35NAIFTKK450 pKa = 10.71LGATGALAATAFHH463 pKa = 7.12IGTAAADD470 pKa = 3.38ASDD473 pKa = 4.11RR474 pKa = 11.84IIYY477 pKa = 10.59NSTTGALIYY486 pKa = 10.36DD487 pKa = 4.31SNGTAAGGATQFATLSKK504 pKa = 10.85GLALTNADD512 pKa = 3.77FLVII516 pKa = 4.33

Molecular weight: 51.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|J2VIT4|J2VIT4_9RHIZ Uncharacterized protein OS=Phyllobacterium sp. YR531 OX=1144343 GN=PMI41_03803 PE=4 SV=1
MM1 pKa = 7.46PRR3 pKa = 11.84SSLKK7 pKa = 10.99YY8 pKa = 8.22IDD10 pKa = 3.41VRR12 pKa = 11.84PRR14 pKa = 11.84PGHH17 pKa = 6.16KK18 pKa = 10.23SQGQLIAGGIVLRR31 pKa = 11.84CALGKK36 pKa = 10.77GGIISRR42 pKa = 11.84KK43 pKa = 9.26RR44 pKa = 11.84EE45 pKa = 3.97GDD47 pKa = 2.93GGTPLASMRR56 pKa = 11.84LLYY59 pKa = 10.59GFKK62 pKa = 10.62RR63 pKa = 11.84PDD65 pKa = 3.15KK66 pKa = 10.11QVRR69 pKa = 11.84ATRR72 pKa = 11.84LLRR75 pKa = 11.84LNPLKK80 pKa = 10.31TNDD83 pKa = 3.15GWCEE87 pKa = 3.64VSNDD91 pKa = 3.22RR92 pKa = 11.84NYY94 pKa = 10.71NRR96 pKa = 11.84QVQIPYY102 pKa = 9.22NASHH106 pKa = 5.52EE107 pKa = 4.57TMWRR111 pKa = 11.84KK112 pKa = 10.26DD113 pKa = 3.77DD114 pKa = 5.27LYY116 pKa = 10.93DD117 pKa = 3.36ICIVLDD123 pKa = 3.34WNIRR127 pKa = 11.84PRR129 pKa = 11.84RR130 pKa = 11.84RR131 pKa = 11.84AGGSAIFFHH140 pKa = 6.99LARR143 pKa = 11.84PQYY146 pKa = 10.04TPTEE150 pKa = 3.87GCIALNRR157 pKa = 11.84ADD159 pKa = 4.07MNRR162 pKa = 11.84LLPHH166 pKa = 7.03LSDD169 pKa = 3.17KK170 pKa = 10.76TVIRR174 pKa = 11.84VLRR177 pKa = 4.06

Molecular weight: 20.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4844

0

4844

1500639

18

5517

309.8

33.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.004 ± 0.044

0.733 ± 0.01

5.657 ± 0.03

5.682 ± 0.037

4.002 ± 0.024

8.188 ± 0.04

1.972 ± 0.019

6.231 ± 0.027

4.221 ± 0.032

9.875 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.459 ± 0.016

3.346 ± 0.031

4.708 ± 0.024

3.252 ± 0.022

6.062 ± 0.039

6.128 ± 0.028

5.562 ± 0.047

7.199 ± 0.03

1.278 ± 0.015

2.44 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski