Amino acid dipepetide frequency for Phyllobacterium sp. YR531

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	13.156AlaAla: 13.156 ± 0.12	0.793AlaCys: 0.793 ± 0.026	6.053AlaAsp: 6.053 ± 0.073	6.42AlaGlu: 6.42 ± 0.074	4.347AlaPhe: 4.347 ± 0.057	9.36AlaGly: 9.36 ± 0.087	1.929AlaHis: 1.929 ± 0.039	7.043AlaIle: 7.043 ± 0.076	4.99AlaLys: 4.99 ± 0.078	11.47AlaLeu: 11.47 ± 0.103	3.111AlaMet: 3.111 ± 0.043	3.479AlaAsn: 3.479 ± 0.063	4.381AlaPro: 4.381 ± 0.07	3.439AlaGln: 3.439 ± 0.053	6.325AlaArg: 6.325 ± 0.073	6.505AlaSer: 6.505 ± 0.075	5.726AlaThr: 5.726 ± 0.077	7.774AlaVal: 7.774 ± 0.079	1.201AlaTrp: 1.201 ± 0.032	2.54AlaTyr: 2.54 ± 0.043	0.0AlaXaa: 0.0 ± 0.0
Cys	0.76CysAla: 0.76 ± 0.025	0.092CysCys: 0.092 ± 0.007	0.433CysAsp: 0.433 ± 0.019	0.387CysGlu: 0.387 ± 0.018	0.31CysPhe: 0.31 ± 0.013	0.76CysGly: 0.76 ± 0.024	0.195CysHis: 0.195 ± 0.011	0.438CysIle: 0.438 ± 0.017	0.223CysLys: 0.223 ± 0.01	0.703CysLeu: 0.703 ± 0.022	0.154CysMet: 0.154 ± 0.01	0.206CysAsn: 0.206 ± 0.013	0.353CysPro: 0.353 ± 0.016	0.214CysGln: 0.214 ± 0.011	0.435CysArg: 0.435 ± 0.016	0.483CysSer: 0.483 ± 0.02	0.396CysThr: 0.396 ± 0.015	0.505CysVal: 0.505 ± 0.018	0.105CysTrp: 0.105 ± 0.008	0.177CysTyr: 0.177 ± 0.01	0.0CysXaa: 0.0 ± 0.0
Asp	6.049AspAla: 6.049 ± 0.081	0.428AspCys: 0.428 ± 0.02	3.178AspAsp: 3.178 ± 0.048	3.53AspGlu: 3.53 ± 0.053	2.292AspPhe: 2.292 ± 0.045	4.851AspGly: 4.851 ± 0.079	1.229AspHis: 1.229 ± 0.03	3.653AspIle: 3.653 ± 0.048	2.27AspLys: 2.27 ± 0.044	5.427AspLeu: 5.427 ± 0.066	1.375AspMet: 1.375 ± 0.034	1.778AspAsn: 1.778 ± 0.04	2.997AspPro: 2.997 ± 0.048	1.863AspGln: 1.863 ± 0.037	3.746AspArg: 3.746 ± 0.062	2.454AspSer: 2.454 ± 0.048	2.851AspThr: 2.851 ± 0.067	4.068AspVal: 4.068 ± 0.058	0.938AspTrp: 0.938 ± 0.026	1.596AspTyr: 1.596 ± 0.032	0.0AspXaa: 0.0 ± 0.0
Glu	6.483GluAla: 6.483 ± 0.083	0.327GluCys: 0.327 ± 0.018	2.773GluAsp: 2.773 ± 0.052	3.264GluGlu: 3.264 ± 0.065	1.951GluPhe: 1.951 ± 0.037	3.913GluGly: 3.913 ± 0.057	1.197GluHis: 1.197 ± 0.028	3.934GluIle: 3.934 ± 0.057	3.086GluLys: 3.086 ± 0.052	5.469GluLeu: 5.469 ± 0.077	1.485GluMet: 1.485 ± 0.031	2.196GluAsn: 2.196 ± 0.038	2.528GluPro: 2.528 ± 0.045	2.283GluGln: 2.283 ± 0.042	4.254GluArg: 4.254 ± 0.06	2.64GluSer: 2.64 ± 0.045	3.543GluThr: 3.543 ± 0.056	3.585GluVal: 3.585 ± 0.053	0.734GluTrp: 0.734 ± 0.022	1.177GluTyr: 1.177 ± 0.03	0.0GluXaa: 0.0 ± 0.0
Phe	4.387PheAla: 4.387 ± 0.062	0.366PheCys: 0.366 ± 0.017	2.791PheAsp: 2.791 ± 0.045	2.29PheGlu: 2.29 ± 0.037	1.667PhePhe: 1.667 ± 0.042	3.866PheGly: 3.866 ± 0.055	0.795PheHis: 0.795 ± 0.024	2.293PheIle: 2.293 ± 0.046	1.347PheLys: 1.347 ± 0.032	3.622PheLeu: 3.622 ± 0.054	0.871PheMet: 0.871 ± 0.028	1.379PheAsn: 1.379 ± 0.033	1.598PhePro: 1.598 ± 0.033	1.15PheGln: 1.15 ± 0.024	2.14PheArg: 2.14 ± 0.043	2.683PheSer: 2.683 ± 0.046	2.179PheThr: 2.179 ± 0.048	2.94PheVal: 2.94 ± 0.046	0.581PheTrp: 0.581 ± 0.02	1.077PheTyr: 1.077 ± 0.026	0.0PheXaa: 0.0 ± 0.0
Gly	7.901GlyAla: 7.901 ± 0.087	0.702GlyCys: 0.702 ± 0.02	4.221GlyAsp: 4.221 ± 0.055	4.369GlyGlu: 4.369 ± 0.062	3.72GlyPhe: 3.72 ± 0.055	6.729GlyGly: 6.729 ± 0.112	1.681GlyHis: 1.681 ± 0.04	5.376GlyIle: 5.376 ± 0.071	4.115GlyLys: 4.115 ± 0.06	8.374GlyLeu: 8.374 ± 0.089	2.124GlyMet: 2.124 ± 0.036	2.773GlyAsn: 2.773 ± 0.067	3.011GlyPro: 3.011 ± 0.05	2.799GlyGln: 2.799 ± 0.042	4.843GlyArg: 4.843 ± 0.058	5.113GlySer: 5.113 ± 0.067	4.711GlyThr: 4.711 ± 0.12	5.739GlyVal: 5.739 ± 0.067	1.239GlyTrp: 1.239 ± 0.034	2.336GlyTyr: 2.336 ± 0.047	0.0GlyXaa: 0.0 ± 0.0
His	1.988HisAla: 1.988 ± 0.035	0.207HisCys: 0.207 ± 0.012	1.207HisAsp: 1.207 ± 0.026	1.028HisGlu: 1.028 ± 0.027	0.929HisPhe: 0.929 ± 0.03	1.729HisGly: 1.729 ± 0.03	0.564HisHis: 0.564 ± 0.023	1.16HisIle: 1.16 ± 0.031	0.642HisLys: 0.642 ± 0.024	1.997HisLeu: 1.997 ± 0.042	0.494HisMet: 0.494 ± 0.017	0.584HisAsn: 0.584 ± 0.022	1.198HisPro: 1.198 ± 0.029	0.586HisGln: 0.586 ± 0.021	1.193HisArg: 1.193 ± 0.033	1.058HisSer: 1.058 ± 0.031	0.824HisThr: 0.824 ± 0.024	1.411HisVal: 1.411 ± 0.031	0.311HisTrp: 0.311 ± 0.018	0.612HisTyr: 0.612 ± 0.02	0.0HisXaa: 0.0 ± 0.0
Ile	7.967IleAla: 7.967 ± 0.085	0.55IleCys: 0.55 ± 0.02	4.088IleAsp: 4.088 ± 0.061	4.04IleGlu: 4.04 ± 0.062	2.27IlePhe: 2.27 ± 0.045	5.587IleGly: 5.587 ± 0.077	1.078IleHis: 1.078 ± 0.024	3.717IleIle: 3.717 ± 0.061	2.205IleLys: 2.205 ± 0.043	5.497IleLeu: 5.497 ± 0.077	1.247IleMet: 1.247 ± 0.03	2.118IleAsn: 2.118 ± 0.045	2.661IlePro: 2.661 ± 0.048	1.515IleGln: 1.515 ± 0.033	3.441IleArg: 3.441 ± 0.045	4.044IleSer: 4.044 ± 0.055	3.33IleThr: 3.33 ± 0.057	4.823IleVal: 4.823 ± 0.067	0.749IleTrp: 0.749 ± 0.023	1.385IleTyr: 1.385 ± 0.031	0.0IleXaa: 0.0 ± 0.0
Lys	4.849LysAla: 4.849 ± 0.063	0.181LysCys: 0.181 ± 0.011	2.254LysAsp: 2.254 ± 0.041	2.18LysGlu: 2.18 ± 0.048	1.322LysPhe: 1.322 ± 0.031	3.088LysGly: 3.088 ± 0.051	0.795LysHis: 0.795 ± 0.024	2.518LysIle: 2.518 ± 0.038	1.972LysLys: 1.972 ± 0.042	4.222LysLeu: 4.222 ± 0.065	0.983LysMet: 0.983 ± 0.026	1.501LysAsn: 1.501 ± 0.035	2.5LysPro: 2.5 ± 0.053	1.489LysGln: 1.489 ± 0.033	2.766LysArg: 2.766 ± 0.051	2.759LysSer: 2.759 ± 0.046	2.624LysThr: 2.624 ± 0.043	2.886LysVal: 2.886 ± 0.047	0.45LysTrp: 0.45 ± 0.017	0.868LysTyr: 0.868 ± 0.024	0.0LysXaa: 0.0 ± 0.0
Leu	11.584LeuAla: 11.584 ± 0.095	0.806LeuCys: 0.806 ± 0.023	5.69LeuAsp: 5.69 ± 0.068	5.254LeuGlu: 5.254 ± 0.07	3.787LeuPhe: 3.787 ± 0.064	7.84LeuGly: 7.84 ± 0.092	1.783LeuHis: 1.783 ± 0.038	5.912LeuIle: 5.912 ± 0.071	4.395LeuLys: 4.395 ± 0.061	9.27LeuLeu: 9.27 ± 0.121	2.282LeuMet: 2.282 ± 0.045	3.141LeuAsn: 3.141 ± 0.045	5.144LeuPro: 5.144 ± 0.064	3.025LeuGln: 3.025 ± 0.048	5.758LeuArg: 5.758 ± 0.074	6.947LeuSer: 6.947 ± 0.079	5.691LeuThr: 5.691 ± 0.073	7.192LeuVal: 7.192 ± 0.083	1.037LeuTrp: 1.037 ± 0.03	2.209LeuTyr: 2.209 ± 0.039	0.0LeuXaa: 0.0 ± 0.0
Met	2.791MetAla: 2.791 ± 0.048	0.143MetCys: 0.143 ± 0.008	1.106MetAsp: 1.106 ± 0.029	1.112MetGlu: 1.112 ± 0.028	0.782MetPhe: 0.782 ± 0.025	1.715MetGly: 1.715 ± 0.036	0.452MetHis: 0.452 ± 0.017	1.617MetIle: 1.617 ± 0.031	1.128MetLys: 1.128 ± 0.032	2.426MetLeu: 2.426 ± 0.04	0.656MetMet: 0.656 ± 0.021	0.958MetAsn: 0.958 ± 0.026	1.357MetPro: 1.357 ± 0.034	0.891MetGln: 0.891 ± 0.024	1.644MetArg: 1.644 ± 0.034	1.722MetSer: 1.722 ± 0.033	1.881MetThr: 1.881 ± 0.034	1.664MetVal: 1.664 ± 0.035	0.213MetTrp: 0.213 ± 0.011	0.333MetTyr: 0.333 ± 0.013	0.0MetXaa: 0.0 ± 0.0
Asn	3.672AsnAla: 3.672 ± 0.062	0.243AsnCys: 0.243 ± 0.014	2.005AsnAsp: 2.005 ± 0.066	1.743AsnGlu: 1.743 ± 0.035	1.259AsnPhe: 1.259 ± 0.03	3.013AsnGly: 3.013 ± 0.087	0.643AsnHis: 0.643 ± 0.022	2.08AsnIle: 2.08 ± 0.041	1.11AsnLys: 1.11 ± 0.026	3.161AsnLeu: 3.161 ± 0.048	0.76AsnMet: 0.76 ± 0.024	1.137AsnAsn: 1.137 ± 0.031	2.113AsnPro: 2.113 ± 0.043	0.992AsnGln: 0.992 ± 0.028	2.052AsnArg: 2.052 ± 0.037	1.901AsnSer: 1.901 ± 0.04	1.661AsnThr: 1.661 ± 0.037	2.465AsnVal: 2.465 ± 0.053	0.548AsnTrp: 0.548 ± 0.018	0.904AsnTyr: 0.904 ± 0.026	0.0AsnXaa: 0.0 ± 0.0
Pro	5.065ProAla: 5.065 ± 0.078	0.265ProCys: 0.265 ± 0.014	3.262ProAsp: 3.262 ± 0.055	3.548ProGlu: 3.548 ± 0.052	1.99ProPhe: 1.99 ± 0.037	3.666ProGly: 3.666 ± 0.049	0.998ProHis: 0.998 ± 0.028	2.533ProIle: 2.533 ± 0.041	1.952ProLys: 1.952 ± 0.036	4.518ProLeu: 4.518 ± 0.058	1.029ProMet: 1.029 ± 0.025	1.507ProAsn: 1.507 ± 0.03	1.772ProPro: 1.772 ± 0.04	1.617ProGln: 1.617 ± 0.034	2.222ProArg: 2.222 ± 0.049	2.765ProSer: 2.765 ± 0.042	2.358ProThr: 2.358 ± 0.049	4.157ProVal: 4.157 ± 0.067	0.612ProTrp: 0.612 ± 0.022	1.249ProTyr: 1.249 ± 0.032	0.0ProXaa: 0.0 ± 0.0
Gln	3.662GlnAla: 3.662 ± 0.057	0.187GlnCys: 0.187 ± 0.011	1.424GlnAsp: 1.424 ± 0.033	1.598GlnGlu: 1.598 ± 0.036	1.269GlnPhe: 1.269 ± 0.028	2.236GlnGly: 2.236 ± 0.04	0.648GlnHis: 0.648 ± 0.022	2.155GlnIle: 2.155 ± 0.044	1.471GlnLys: 1.471 ± 0.033	3.197GlnLeu: 3.197 ± 0.043	0.896GlnMet: 0.896 ± 0.025	1.17GlnAsn: 1.17 ± 0.028	1.629GlnPro: 1.629 ± 0.036	1.322GlnGln: 1.322 ± 0.039	2.305GlnArg: 2.305 ± 0.042	2.181GlnSer: 2.181 ± 0.04	1.921GlnThr: 1.921 ± 0.039	2.126GlnVal: 2.126 ± 0.04	0.392GlnTrp: 0.392 ± 0.017	0.735GlnTyr: 0.735 ± 0.024	0.0GlnXaa: 0.0 ± 0.0
Arg	5.803ArgAla: 5.803 ± 0.075	0.375ArgCys: 0.375 ± 0.017	3.484ArgAsp: 3.484 ± 0.05	3.648ArgGlu: 3.648 ± 0.054	2.74ArgPhe: 2.74 ± 0.042	3.889ArgGly: 3.889 ± 0.058	1.378ArgHis: 1.378 ± 0.035	4.048ArgIle: 4.048 ± 0.056	2.585ArgLys: 2.585 ± 0.044	6.583ArgLeu: 6.583 ± 0.076	1.647ArgMet: 1.647 ± 0.034	2.166ArgAsn: 2.166 ± 0.037	2.712ArgPro: 2.712 ± 0.049	2.385ArgGln: 2.385 ± 0.046	4.018ArgArg: 4.018 ± 0.068	3.688ArgSer: 3.688 ± 0.058	3.087ArgThr: 3.087 ± 0.048	3.948ArgVal: 3.948 ± 0.05	0.79ArgTrp: 0.79 ± 0.026	1.643ArgTyr: 1.643 ± 0.037	0.0ArgXaa: 0.0 ± 0.0
Ser	6.357SerAla: 6.357 ± 0.068	0.394SerCys: 0.394 ± 0.019	3.403SerAsp: 3.403 ± 0.056	3.265SerGlu: 3.265 ± 0.05	2.672SerPhe: 2.672 ± 0.045	6.027SerGly: 6.027 ± 0.077	1.209SerHis: 1.209 ± 0.029	3.806SerIle: 3.806 ± 0.058	2.38SerLys: 2.38 ± 0.04	5.991SerLeu: 5.991 ± 0.071	1.561SerMet: 1.561 ± 0.031	2.012SerAsn: 2.012 ± 0.037	2.82SerPro: 2.82 ± 0.046	1.906SerGln: 1.906 ± 0.042	3.606SerArg: 3.606 ± 0.051	3.688SerSer: 3.688 ± 0.061	3.214SerThr: 3.214 ± 0.052	4.524SerVal: 4.524 ± 0.065	0.841SerTrp: 0.841 ± 0.026	1.605SerTyr: 1.605 ± 0.031	0.0SerXaa: 0.0 ± 0.0
Thr	6.049ThrAla: 6.049 ± 0.09	0.391ThrCys: 0.391 ± 0.016	3.011ThrAsp: 3.011 ± 0.049	2.804ThrGlu: 2.804 ± 0.046	2.163ThrPhe: 2.163 ± 0.044	5.212ThrGly: 5.212 ± 0.078	1.054ThrHis: 1.054 ± 0.023	3.671ThrIle: 3.671 ± 0.064	2.054ThrLys: 2.054 ± 0.035	5.805ThrLeu: 5.805 ± 0.068	1.251ThrMet: 1.251 ± 0.033	1.703ThrAsn: 1.703 ± 0.049	3.135ThrPro: 3.135 ± 0.06	1.562ThrGln: 1.562 ± 0.039	3.019ThrArg: 3.019 ± 0.042	3.365ThrSer: 3.365 ± 0.053	3.106ThrThr: 3.106 ± 0.063	4.279ThrVal: 4.279 ± 0.097	0.622ThrTrp: 0.622 ± 0.019	1.364ThrTyr: 1.364 ± 0.043	0.0ThrXaa: 0.0 ± 0.0
Val	7.979ValAla: 7.979 ± 0.077	0.544ValCys: 0.544 ± 0.019	4.024ValAsp: 4.024 ± 0.054	4.357ValGlu: 4.357 ± 0.063	2.982ValPhe: 2.982 ± 0.041	5.324ValGly: 5.324 ± 0.067	1.316ValHis: 1.316 ± 0.032	4.425ValIle: 4.425 ± 0.064	2.881ValLys: 2.881 ± 0.046	6.973ValLeu: 6.973 ± 0.073	1.795ValMet: 1.795 ± 0.039	2.329ValAsn: 2.329 ± 0.058	3.407ValPro: 3.407 ± 0.045	2.097ValGln: 2.097 ± 0.04	4.26ValArg: 4.26 ± 0.055	4.903ValSer: 4.903 ± 0.066	4.513ValThr: 4.513 ± 0.091	5.481ValVal: 5.481 ± 0.071	0.836ValTrp: 0.836 ± 0.025	1.565ValTyr: 1.565 ± 0.031	0.0ValXaa: 0.0 ± 0.0
Trp	1.115TrpAla: 1.115 ± 0.027	0.11TrpCys: 0.11 ± 0.008	0.59TrpAsp: 0.59 ± 0.023	0.561TrpGlu: 0.561 ± 0.022	0.535TrpPhe: 0.535 ± 0.02	0.85TrpGly: 0.85 ± 0.027	0.282TrpHis: 0.282 ± 0.015	0.726TrpIle: 0.726 ± 0.023	0.538TrpLys: 0.538 ± 0.019	1.574TrpLeu: 1.574 ± 0.038	0.357TrpMet: 0.357 ± 0.015	0.523TrpAsn: 0.523 ± 0.02	0.613TrpPro: 0.613 ± 0.02	0.576TrpGln: 0.576 ± 0.02	0.936TrpArg: 0.936 ± 0.027	0.858TrpSer: 0.858 ± 0.028	0.728TrpThr: 0.728 ± 0.022	0.771TrpVal: 0.771 ± 0.025	0.215TrpTrp: 0.215 ± 0.013	0.319TrpTyr: 0.319 ± 0.014	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.426TyrAla: 2.426 ± 0.042	0.226TyrCys: 0.226 ± 0.011	1.573TyrAsp: 1.573 ± 0.033	1.424TyrGlu: 1.424 ± 0.031	1.035TyrPhe: 1.035 ± 0.029	2.223TyrGly: 2.223 ± 0.045	0.499TyrHis: 0.499 ± 0.019	1.207TyrIle: 1.207 ± 0.032	0.863TyrLys: 0.863 ± 0.024	2.468TyrLeu: 2.468 ± 0.035	0.508TyrMet: 0.508 ± 0.02	0.802TyrAsn: 0.802 ± 0.025	1.156TyrPro: 1.156 ± 0.029	0.816TyrGln: 0.816 ± 0.024	1.652TyrArg: 1.652 ± 0.031	1.523TyrSer: 1.523 ± 0.041	1.277TyrThr: 1.277 ± 0.041	1.653TyrVal: 1.653 ± 0.037	0.364TyrTrp: 0.364 ± 0.014	0.706TyrTyr: 0.706 ± 0.022	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4844 proteins (1500639 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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