Streptomyces sp. CNQ-509

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6367 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0F7N856|A0A0F7N856_9ACTN Uncharacterized protein OS=Streptomyces sp. CNQ-509 OX=444103 GN=AA958_17035 PE=3 SV=1
MM1 pKa = 7.95WINPNNGFGFACTTCSTTTYY21 pKa = 9.57EE22 pKa = 4.13RR23 pKa = 11.84QEE25 pKa = 3.88MTVLEE30 pKa = 4.35EE31 pKa = 4.63TNNHH35 pKa = 5.13WKK37 pKa = 8.87TGAKK41 pKa = 9.74QILAVGDD48 pKa = 3.38VDD50 pKa = 5.74GPLDD54 pKa = 3.79TDD56 pKa = 4.33DD57 pKa = 5.75DD58 pKa = 4.99GEE60 pKa = 5.19IDD62 pKa = 3.73TPGYY66 pKa = 9.83PDD68 pKa = 5.01LLVNDD73 pKa = 5.11GQHH76 pKa = 5.21LWLYY80 pKa = 10.23YY81 pKa = 10.53GDD83 pKa = 4.65PGGSAYY89 pKa = 10.83LDD91 pKa = 3.52AFRR94 pKa = 11.84DD95 pKa = 4.01PVLLAAGDD103 pKa = 4.0GMSTGTNTLANVTMAAPGDD122 pKa = 3.89FDD124 pKa = 6.34ADD126 pKa = 3.28GHH128 pKa = 6.84ADD130 pKa = 3.36LTVRR134 pKa = 11.84FDD136 pKa = 3.74NDD138 pKa = 3.16GSGLFLYY145 pKa = 10.51DD146 pKa = 5.39AINPDD151 pKa = 3.26ASTWPGQIDD160 pKa = 3.59PTHH163 pKa = 7.26RR164 pKa = 11.84ILIANNFGPNTVPMLTAAPDD184 pKa = 3.62ANNNGTFDD192 pKa = 4.02LWTTTPNSGRR202 pKa = 11.84LRR204 pKa = 11.84FFADD208 pKa = 4.56FTPDD212 pKa = 3.05GPVAITVASEE222 pKa = 3.63QFANYY227 pKa = 8.82QALGG231 pKa = 3.47

Molecular weight: 24.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0F7N7Z8|A0A0F7N7Z8_9ACTN Probable M18 family aminopeptidase 2 OS=Streptomyces sp. CNQ-509 OX=444103 GN=apeB PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84KK12 pKa = 8.98RR13 pKa = 11.84AKK15 pKa = 8.46THH17 pKa = 5.11GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AIITARR35 pKa = 11.84RR36 pKa = 11.84TKK38 pKa = 10.43GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6367

0

6367

2181675

29

5986

342.7

36.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.6 ± 0.052

0.745 ± 0.008

6.065 ± 0.025

5.872 ± 0.028

2.658 ± 0.017

10.001 ± 0.034

2.236 ± 0.02

2.882 ± 0.023

1.867 ± 0.025

10.199 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.696 ± 0.014

1.596 ± 0.015

6.353 ± 0.03

2.575 ± 0.017

8.311 ± 0.041

4.551 ± 0.021

5.878 ± 0.026

8.322 ± 0.027

1.476 ± 0.013

2.119 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski