Amino acid dipepetide frequency for Streptomyces sp. CNQ-509

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
26.18AlaAla: 26.18 ± 0.208
1.068AlaCys: 1.068 ± 0.024
9.069AlaAsp: 9.069 ± 0.069
10.028AlaGlu: 10.028 ± 0.087
3.463AlaPhe: 3.463 ± 0.046
16.094AlaGly: 16.094 ± 0.115
2.847AlaHis: 2.847 ± 0.045
3.226AlaIle: 3.226 ± 0.04
2.457AlaLys: 2.457 ± 0.049
14.736AlaLeu: 14.736 ± 0.124
2.519AlaMet: 2.519 ± 0.037
1.812AlaAsn: 1.812 ± 0.032
7.912AlaPro: 7.912 ± 0.09
3.671AlaGln: 3.671 ± 0.047
11.157AlaArg: 11.157 ± 0.097
5.209AlaSer: 5.209 ± 0.05
6.891AlaThr: 6.891 ± 0.071
12.836AlaVal: 12.836 ± 0.097
1.806AlaTrp: 1.806 ± 0.034
3.024AlaTyr: 3.024 ± 0.042
0.0AlaXaa: 0.0 ± 0.0
Cys
1.095CysAla: 1.095 ± 0.023
0.094CysCys: 0.094 ± 0.007
0.452CysAsp: 0.452 ± 0.015
0.362CysGlu: 0.362 ± 0.014
0.205CysPhe: 0.205 ± 0.01
0.899CysGly: 0.899 ± 0.022
0.182CysHis: 0.182 ± 0.009
0.173CysIle: 0.173 ± 0.009
0.107CysLys: 0.107 ± 0.007
0.679CysLeu: 0.679 ± 0.02
0.13CysMet: 0.13 ± 0.008
0.131CysAsn: 0.131 ± 0.008
0.446CysPro: 0.446 ± 0.014
0.141CysGln: 0.141 ± 0.008
0.594CysArg: 0.594 ± 0.017
0.386CysSer: 0.386 ± 0.013
0.511CysThr: 0.511 ± 0.016
0.609CysVal: 0.609 ± 0.015
0.116CysTrp: 0.116 ± 0.007
0.14CysTyr: 0.14 ± 0.008
0.0CysXaa: 0.0 ± 0.0
Asp
8.273AspAla: 8.273 ± 0.072
0.405AspCys: 0.405 ± 0.013
3.859AspAsp: 3.859 ± 0.046
4.02AspGlu: 4.02 ± 0.051
1.686AspPhe: 1.686 ± 0.03
6.947AspGly: 6.947 ± 0.074
1.366AspHis: 1.366 ± 0.029
1.71AspIle: 1.71 ± 0.032
1.075AspLys: 1.075 ± 0.026
5.961AspLeu: 5.961 ± 0.055
0.831AspMet: 0.831 ± 0.021
0.897AspAsn: 0.897 ± 0.025
4.692AspPro: 4.692 ± 0.066
1.343AspGln: 1.343 ± 0.025
4.965AspArg: 4.965 ± 0.054
2.33AspSer: 2.33 ± 0.035
3.126AspThr: 3.126 ± 0.04
4.952AspVal: 4.952 ± 0.055
1.012AspTrp: 1.012 ± 0.023
1.2AspTyr: 1.2 ± 0.025
0.0AspXaa: 0.0 ± 0.0
Glu
7.642GluAla: 7.642 ± 0.07
0.368GluCys: 0.368 ± 0.013
2.99GluAsp: 2.99 ± 0.039
3.745GluGlu: 3.745 ± 0.056
1.54GluPhe: 1.54 ± 0.03
4.497GluGly: 4.497 ± 0.052
1.551GluHis: 1.551 ± 0.029
2.085GluIle: 2.085 ± 0.033
1.433GluLys: 1.433 ± 0.032
7.084GluLeu: 7.084 ± 0.063
0.919GluMet: 0.919 ± 0.022
0.978GluAsn: 0.978 ± 0.025
3.892GluPro: 3.892 ± 0.052
2.146GluGln: 2.146 ± 0.035
5.733GluArg: 5.733 ± 0.062
2.588GluSer: 2.588 ± 0.037
2.963GluThr: 2.963 ± 0.044
4.651GluVal: 4.651 ± 0.057
0.796GluTrp: 0.796 ± 0.018
1.119GluTyr: 1.119 ± 0.026
0.0GluXaa: 0.0 ± 0.0
Phe
3.902PheAla: 3.902 ± 0.038
0.268PheCys: 0.268 ± 0.011
1.942PheAsp: 1.942 ± 0.035
1.354PheGlu: 1.354 ± 0.028
0.849PhePhe: 0.849 ± 0.021
2.934PheGly: 2.934 ± 0.041
0.601PheHis: 0.601 ± 0.019
0.66PheIle: 0.66 ± 0.017
0.414PheLys: 0.414 ± 0.015
2.512PheLeu: 2.512 ± 0.044
0.385PheMet: 0.385 ± 0.015
0.51PheAsn: 0.51 ± 0.014
1.343PhePro: 1.343 ± 0.027
0.599PheGln: 0.599 ± 0.015
1.85PheArg: 1.85 ± 0.03
1.341PheSer: 1.341 ± 0.028
2.02PheThr: 2.02 ± 0.037
2.142PheVal: 2.142 ± 0.033
0.41PheTrp: 0.41 ± 0.015
0.545PheTyr: 0.545 ± 0.016
0.0PheXaa: 0.0 ± 0.0
Gly
12.804GlyAla: 12.804 ± 0.111
0.801GlyCys: 0.801 ± 0.024
5.71GlyAsp: 5.71 ± 0.069
5.844GlyGlu: 5.844 ± 0.053
2.818GlyPhe: 2.818 ± 0.04
10.473GlyGly: 10.473 ± 0.126
2.262GlyHis: 2.262 ± 0.035
3.321GlyIle: 3.321 ± 0.046
2.272GlyLys: 2.272 ± 0.043
9.54GlyLeu: 9.54 ± 0.077
2.11GlyMet: 2.11 ± 0.033
1.691GlyAsn: 1.691 ± 0.036
5.933GlyPro: 5.933 ± 0.075
2.533GlyGln: 2.533 ± 0.035
8.335GlyArg: 8.335 ± 0.073
5.148GlySer: 5.148 ± 0.054
6.589GlyThr: 6.589 ± 0.072
7.672GlyVal: 7.672 ± 0.064
1.767GlyTrp: 1.767 ± 0.033
2.385GlyTyr: 2.385 ± 0.04
0.0GlyXaa: 0.0 ± 0.0
His
2.786HisAla: 2.786 ± 0.04
0.197HisCys: 0.197 ± 0.009
1.329HisAsp: 1.329 ± 0.023
1.187HisGlu: 1.187 ± 0.025
0.615HisPhe: 0.615 ± 0.017
2.417HisGly: 2.417 ± 0.035
0.659HisHis: 0.659 ± 0.024
0.586HisIle: 0.586 ± 0.017
0.289HisLys: 0.289 ± 0.011
2.47HisLeu: 2.47 ± 0.043
0.33HisMet: 0.33 ± 0.013
0.336HisAsn: 0.336 ± 0.015
1.824HisPro: 1.824 ± 0.035
0.54HisGln: 0.54 ± 0.017
2.113HisArg: 2.113 ± 0.032
0.866HisSer: 0.866 ± 0.02
1.273HisThr: 1.273 ± 0.031
1.722HisVal: 1.722 ± 0.026
0.35HisTrp: 0.35 ± 0.013
0.468HisTyr: 0.468 ± 0.017
0.0HisXaa: 0.0 ± 0.0
Ile
4.483IleAla: 4.483 ± 0.05
0.25IleCys: 0.25 ± 0.011
2.072IleAsp: 2.072 ± 0.035
1.825IleGlu: 1.825 ± 0.031
0.668IlePhe: 0.668 ± 0.019
3.219IleGly: 3.219 ± 0.041
0.547IleHis: 0.547 ± 0.017
0.732IleIle: 0.732 ± 0.023
0.612IleLys: 0.612 ± 0.018
2.19IleLeu: 2.19 ± 0.036
0.41IleMet: 0.41 ± 0.014
0.603IleAsn: 0.603 ± 0.018
1.661IlePro: 1.661 ± 0.028
0.615IleGln: 0.615 ± 0.017
2.196IleArg: 2.196 ± 0.036
1.474IleSer: 1.474 ± 0.025
1.933IleThr: 1.933 ± 0.033
2.47IleVal: 2.47 ± 0.037
0.351IleTrp: 0.351 ± 0.012
0.507IleTyr: 0.507 ± 0.016
0.0IleXaa: 0.0 ± 0.0
Lys
2.411LysAla: 2.411 ± 0.042
0.088LysCys: 0.088 ± 0.007
1.152LysAsp: 1.152 ± 0.027
1.177LysGlu: 1.177 ± 0.029
0.376LysPhe: 0.376 ± 0.014
1.519LysGly: 1.519 ± 0.033
0.389LysHis: 0.389 ± 0.015
0.703LysIle: 0.703 ± 0.02
0.711LysLys: 0.711 ± 0.03
1.834LysLeu: 1.834 ± 0.031
0.338LysMet: 0.338 ± 0.014
0.442LysAsn: 0.442 ± 0.017
1.22LysPro: 1.22 ± 0.027
0.614LysGln: 0.614 ± 0.016
1.357LysArg: 1.357 ± 0.026
0.983LysSer: 0.983 ± 0.024
1.131LysThr: 1.131 ± 0.026
1.595LysVal: 1.595 ± 0.031
0.236LysTrp: 0.236 ± 0.012
0.388LysTyr: 0.388 ± 0.015
0.0LysXaa: 0.0 ± 0.0
Leu
16.153LeuAla: 16.153 ± 0.12
0.772LeuCys: 0.772 ± 0.021
6.712LeuAsp: 6.712 ± 0.069
4.582LeuGlu: 4.582 ± 0.046
2.484LeuPhe: 2.484 ± 0.041
9.183LeuGly: 9.183 ± 0.076
2.269LeuHis: 2.269 ± 0.038
2.913LeuIle: 2.913 ± 0.04
1.769LeuLys: 1.769 ± 0.032
11.317LeuLeu: 11.317 ± 0.11
1.553LeuMet: 1.553 ± 0.031
1.493LeuAsn: 1.493 ± 0.029
6.627LeuPro: 6.627 ± 0.06
2.116LeuGln: 2.116 ± 0.033
8.984LeuArg: 8.984 ± 0.078
4.745LeuSer: 4.745 ± 0.051
6.815LeuThr: 6.815 ± 0.067
8.414LeuVal: 8.414 ± 0.068
1.224LeuTrp: 1.224 ± 0.025
1.862LeuTyr: 1.862 ± 0.032
0.0LeuXaa: 0.0 ± 0.0
Met
2.268MetAla: 2.268 ± 0.033
0.14MetCys: 0.14 ± 0.008
0.872MetAsp: 0.872 ± 0.022
0.75MetGlu: 0.75 ± 0.019
0.456MetPhe: 0.456 ± 0.017
1.281MetGly: 1.281 ± 0.027
0.36MetHis: 0.36 ± 0.011
0.592MetIle: 0.592 ± 0.019
0.381MetLys: 0.381 ± 0.013
1.73MetLeu: 1.73 ± 0.029
0.283MetMet: 0.283 ± 0.01
0.371MetAsn: 0.371 ± 0.014
1.136MetPro: 1.136 ± 0.023
0.447MetGln: 0.447 ± 0.015
1.42MetArg: 1.42 ± 0.027
1.214MetSer: 1.214 ± 0.024
1.499MetThr: 1.499 ± 0.028
1.227MetVal: 1.227 ± 0.028
0.206MetTrp: 0.206 ± 0.01
0.324MetTyr: 0.324 ± 0.012
0.0MetXaa: 0.0 ± 0.0
Asn
1.989AsnAla: 1.989 ± 0.034
0.145AsnCys: 0.145 ± 0.009
0.886AsnAsp: 0.886 ± 0.02
0.782AsnGlu: 0.782 ± 0.022
0.456AsnPhe: 0.456 ± 0.015
1.717AsnGly: 1.717 ± 0.03
0.332AsnHis: 0.332 ± 0.011
0.565AsnIle: 0.565 ± 0.019
0.325AsnLys: 0.325 ± 0.015
1.558AsnLeu: 1.558 ± 0.03
0.245AsnMet: 0.245 ± 0.01
0.361AsnAsn: 0.361 ± 0.014
1.257AsnPro: 1.257 ± 0.024
0.426AsnGln: 0.426 ± 0.016
1.166AsnArg: 1.166 ± 0.024
0.818AsnSer: 0.818 ± 0.019
0.963AsnThr: 0.963 ± 0.022
1.294AsnVal: 1.294 ± 0.026
0.275AsnTrp: 0.275 ± 0.013
0.4AsnTyr: 0.4 ± 0.015
0.0AsnXaa: 0.0 ± 0.0
Pro
10.23ProAla: 10.23 ± 0.092
0.328ProCys: 0.328 ± 0.014
4.623ProAsp: 4.623 ± 0.053
4.664ProGlu: 4.664 ± 0.045
1.501ProPhe: 1.501 ± 0.025
7.855ProGly: 7.855 ± 0.086
1.427ProHis: 1.427 ± 0.027
1.07ProIle: 1.07 ± 0.025
0.98ProLys: 0.98 ± 0.025
5.333ProLeu: 5.333 ± 0.06
0.962ProMet: 0.962 ± 0.022
0.769ProAsn: 0.769 ± 0.021
4.349ProPro: 4.349 ± 0.074
1.71ProGln: 1.71 ± 0.036
4.425ProArg: 4.425 ± 0.05
2.654ProSer: 2.654 ± 0.037
2.769ProThr: 2.769 ± 0.043
5.477ProVal: 5.477 ± 0.058
0.895ProTrp: 0.895 ± 0.019
1.505ProTyr: 1.505 ± 0.031
0.0ProXaa: 0.0 ± 0.0
Gln
3.529GlnAla: 3.529 ± 0.043
0.153GlnCys: 0.153 ± 0.008
1.297GlnAsp: 1.297 ± 0.025
1.404GlnGlu: 1.404 ± 0.027
0.659GlnPhe: 0.659 ± 0.02
2.12GlnGly: 2.12 ± 0.033
0.617GlnHis: 0.617 ± 0.02
0.985GlnIle: 0.985 ± 0.023
0.505GlnLys: 0.505 ± 0.018
2.773GlnLeu: 2.773 ± 0.04
0.465GlnMet: 0.465 ± 0.017
0.469GlnAsn: 0.469 ± 0.016
1.683GlnPro: 1.683 ± 0.031
1.184GlnGln: 1.184 ± 0.032
2.361GlnArg: 2.361 ± 0.033
1.147GlnSer: 1.147 ± 0.021
1.241GlnThr: 1.241 ± 0.023
2.203GlnVal: 2.203 ± 0.033
0.402GlnTrp: 0.402 ± 0.014
0.557GlnTyr: 0.557 ± 0.018
0.0GlnXaa: 0.0 ± 0.0
Arg
10.84ArgAla: 10.84 ± 0.1
0.584ArgCys: 0.584 ± 0.017
4.521ArgAsp: 4.521 ± 0.049
5.0ArgGlu: 5.0 ± 0.063
2.273ArgPhe: 2.273 ± 0.033
6.394ArgGly: 6.394 ± 0.066
2.196ArgHis: 2.196 ± 0.039
3.222ArgIle: 3.222 ± 0.038
1.519ArgLys: 1.519 ± 0.029
9.154ArgLeu: 9.154 ± 0.079
1.77ArgMet: 1.77 ± 0.028
1.305ArgAsn: 1.305 ± 0.027
5.222ArgPro: 5.222 ± 0.053
2.287ArgGln: 2.287 ± 0.036
8.452ArgArg: 8.452 ± 0.089
3.712ArgSer: 3.712 ± 0.041
5.438ArgThr: 5.438 ± 0.053
5.98ArgVal: 5.98 ± 0.063
1.419ArgTrp: 1.419 ± 0.025
1.817ArgTyr: 1.817 ± 0.032
0.0ArgXaa: 0.0 ± 0.0
Ser
6.318SerAla: 6.318 ± 0.058
0.349SerCys: 0.349 ± 0.015
2.383SerAsp: 2.383 ± 0.038
2.098SerGlu: 2.098 ± 0.033
1.44SerPhe: 1.44 ± 0.024
5.672SerGly: 5.672 ± 0.062
0.9SerHis: 0.9 ± 0.02
1.189SerIle: 1.189 ± 0.024
0.76SerLys: 0.76 ± 0.022
4.325SerLeu: 4.325 ± 0.046
0.969SerMet: 0.969 ± 0.025
0.729SerAsn: 0.729 ± 0.017
3.007SerPro: 3.007 ± 0.038
1.027SerGln: 1.027 ± 0.025
3.568SerArg: 3.568 ± 0.04
2.347SerSer: 2.347 ± 0.037
2.583SerThr: 2.583 ± 0.036
3.851SerVal: 3.851 ± 0.051
0.829SerTrp: 0.829 ± 0.022
1.167SerTyr: 1.167 ± 0.028
0.0SerXaa: 0.0 ± 0.0
Thr
9.225ThrAla: 9.225 ± 0.091
0.388ThrCys: 0.388 ± 0.015
3.538ThrAsp: 3.538 ± 0.043
3.112ThrGlu: 3.112 ± 0.039
1.562ThrPhe: 1.562 ± 0.025
6.916ThrGly: 6.916 ± 0.066
1.123ThrHis: 1.123 ± 0.026
1.507ThrIle: 1.507 ± 0.031
0.947ThrLys: 0.947 ± 0.023
5.485ThrLeu: 5.485 ± 0.059
0.909ThrMet: 0.909 ± 0.022
0.865ThrAsn: 0.865 ± 0.023
4.006ThrPro: 4.006 ± 0.041
1.211ThrGln: 1.211 ± 0.028
3.897ThrArg: 3.897 ± 0.047
2.778ThrSer: 2.778 ± 0.035
3.479ThrThr: 3.479 ± 0.054
5.634ThrVal: 5.634 ± 0.059
0.859ThrTrp: 0.859 ± 0.023
1.338ThrTyr: 1.338 ± 0.027
0.0ThrXaa: 0.0 ± 0.0
Val
11.209ValAla: 11.209 ± 0.092
0.737ValCys: 0.737 ± 0.017
4.84ValAsp: 4.84 ± 0.055
4.637ValGlu: 4.637 ± 0.052
2.405ValPhe: 2.405 ± 0.034
6.379ValGly: 6.379 ± 0.06
1.949ValHis: 1.949 ± 0.029
2.618ValIle: 2.618 ± 0.037
1.457ValLys: 1.457 ± 0.03
9.497ValLeu: 9.497 ± 0.084
1.321ValMet: 1.321 ± 0.027
1.474ValAsn: 1.474 ± 0.031
5.38ValPro: 5.38 ± 0.059
2.003ValGln: 2.003 ± 0.03
7.267ValArg: 7.267 ± 0.079
4.013ValSer: 4.013 ± 0.045
5.434ValThr: 5.434 ± 0.054
7.824ValVal: 7.824 ± 0.081
1.137ValTrp: 1.137 ± 0.022
1.641ValTyr: 1.641 ± 0.026
0.0ValXaa: 0.0 ± 0.0
Trp
1.64TrpAla: 1.64 ± 0.028
0.143TrpCys: 0.143 ± 0.008
0.831TrpAsp: 0.831 ± 0.021
0.754TrpGlu: 0.754 ± 0.02
0.49TrpPhe: 0.49 ± 0.013
1.035TrpGly: 1.035 ± 0.024
0.384TrpHis: 0.384 ± 0.014
0.528TrpIle: 0.528 ± 0.018
0.307TrpLys: 0.307 ± 0.012
1.766TrpLeu: 1.766 ± 0.034
0.264TrpMet: 0.264 ± 0.012
0.364TrpAsn: 0.364 ± 0.014
0.827TrpPro: 0.827 ± 0.02
0.599TrpGln: 0.599 ± 0.018
1.384TrpArg: 1.384 ± 0.026
0.869TrpSer: 0.869 ± 0.023
0.983TrpThr: 0.983 ± 0.023
0.904TrpVal: 0.904 ± 0.021
0.328TrpTrp: 0.328 ± 0.011
0.355TrpTyr: 0.355 ± 0.014
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.026TyrAla: 3.026 ± 0.041
0.171TyrCys: 0.171 ± 0.008
1.574TyrAsp: 1.574 ± 0.032
1.394TyrGlu: 1.394 ± 0.025
0.633TyrPhe: 0.633 ± 0.018
2.458TyrGly: 2.458 ± 0.032
0.396TyrHis: 0.396 ± 0.015
0.433TyrIle: 0.433 ± 0.014
0.346TyrLys: 0.346 ± 0.014
2.045TyrLeu: 2.045 ± 0.033
0.245TyrMet: 0.245 ± 0.011
0.36TyrAsn: 0.36 ± 0.015
1.107TyrPro: 1.107 ± 0.023
0.542TyrGln: 0.542 ± 0.017
1.884TyrArg: 1.884 ± 0.032
0.889TyrSer: 0.889 ± 0.02
1.137TyrThr: 1.137 ± 0.026
1.764TyrVal: 1.764 ± 0.026
0.339TyrTrp: 0.339 ± 0.014
0.445TyrTyr: 0.445 ± 0.017
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 6367 proteins (2181675 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski