Jannaschia helgolandensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Jannaschia

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3471 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H7G164|A0A1H7G164_9RHOB Farnesyl-diphosphate synthase OS=Jannaschia helgolandensis OX=188906 GN=SAMN04488526_0275 PE=3 SV=1
MM1 pKa = 7.15PTIDD5 pKa = 4.75GSISDD10 pKa = 4.04WSPDD14 pKa = 3.14ARR16 pKa = 11.84LDD18 pKa = 3.6TAATGTLGYY27 pKa = 9.98GLWGVADD34 pKa = 3.74PDD36 pKa = 3.72YY37 pKa = 10.8FYY39 pKa = 11.2FAITSDD45 pKa = 3.56DD46 pKa = 4.01VIIGEE51 pKa = 4.38NTTLWLDD58 pKa = 3.38TDD60 pKa = 4.52LDD62 pKa = 4.15RR63 pKa = 11.84GTGYY67 pKa = 10.33QIWGWAGGAEE77 pKa = 4.23YY78 pKa = 10.1QVEE81 pKa = 4.08IAADD85 pKa = 3.77GTARR89 pKa = 11.84LYY91 pKa = 11.13SGGPEE96 pKa = 3.79EE97 pKa = 4.18TLVAEE102 pKa = 4.59LEE104 pKa = 4.44AVRR107 pKa = 11.84SADD110 pKa = 3.14GRR112 pKa = 11.84VLEE115 pKa = 4.11FRR117 pKa = 11.84IDD119 pKa = 3.51RR120 pKa = 11.84SLLAGNPDD128 pKa = 3.47AVRR131 pKa = 11.84VYY133 pKa = 11.62ADD135 pKa = 3.52VNDD138 pKa = 4.12SAFLPNSYY146 pKa = 11.09ANTNFIVAEE155 pKa = 4.11PSPSVVTGGKK165 pKa = 7.65TLDD168 pKa = 3.85GNLVDD173 pKa = 3.99WDD175 pKa = 3.6AATRR179 pKa = 11.84LDD181 pKa = 3.95TPATGAAGYY190 pKa = 10.63AFYY193 pKa = 11.34GDD195 pKa = 3.83LQDD198 pKa = 4.49NVFTFAISTDD208 pKa = 3.18GTAIGANTTIWLDD221 pKa = 3.44TDD223 pKa = 3.9RR224 pKa = 11.84DD225 pKa = 3.89TATGYY230 pKa = 8.53QVWGWAVGGEE240 pKa = 4.05YY241 pKa = 10.42NINITADD248 pKa = 3.38GEE250 pKa = 4.19ARR252 pKa = 11.84LYY254 pKa = 10.24TGAAGEE260 pKa = 4.38TFVANLDD267 pKa = 3.43FAYY270 pKa = 10.71NADD273 pKa = 3.52GTVIEE278 pKa = 4.49IALDD282 pKa = 3.45RR283 pKa = 11.84ALVGNPASMRR293 pKa = 11.84VFADD297 pKa = 3.29VNDD300 pKa = 3.96SVFIPNSYY308 pKa = 11.22ANANFIVGSEE318 pKa = 4.01PTPVIGGITLDD329 pKa = 3.78GSVSEE334 pKa = 4.39WTPEE338 pKa = 4.57SILLTQGGYY347 pKa = 6.9QLRR350 pKa = 11.84GEE352 pKa = 4.53FVVDD356 pKa = 3.65AAGDD360 pKa = 3.58QYY362 pKa = 11.94VFAIEE367 pKa = 4.51SDD369 pKa = 3.43GTTIGANTTIWLDD382 pKa = 3.14TDD384 pKa = 4.36LNSTTGYY391 pKa = 10.2QIWGWAGGAEE401 pKa = 4.19YY402 pKa = 10.63NVNITADD409 pKa = 3.62GQARR413 pKa = 11.84LYY415 pKa = 10.83SGAAGGTYY423 pKa = 9.95IADD426 pKa = 3.77LDD428 pKa = 4.35FGFSADD434 pKa = 3.38GTGFEE439 pKa = 4.36VAISKK444 pKa = 10.45DD445 pKa = 4.02LLSGAPDD452 pKa = 3.35QVRR455 pKa = 11.84ILADD459 pKa = 3.3VNDD462 pKa = 4.1AVFLPGDD469 pKa = 3.63YY470 pKa = 10.8SSANLVVGAPLPPPTDD486 pKa = 3.52APEE489 pKa = 3.74SRR491 pKa = 11.84IAIVYY496 pKa = 9.67SATTAANFYY505 pKa = 10.71DD506 pKa = 3.16ITAYY510 pKa = 10.4GQLFMAAQNQAMQAGIPFDD529 pKa = 4.5LLTEE533 pKa = 4.69ADD535 pKa = 4.33LLDD538 pKa = 3.9PTGLAGYY545 pKa = 9.43DD546 pKa = 3.29AIVFPGFSNVQASQAATIAASLTTAAQDD574 pKa = 3.52YY575 pKa = 8.71GVGMIVAGNFLTNDD589 pKa = 3.34EE590 pKa = 4.66TGAALPGNSYY600 pKa = 11.51ARR602 pKa = 11.84MNALLGVTLEE612 pKa = 4.69GFGATQGITLNASNAANPILDD633 pKa = 4.61GYY635 pKa = 9.62TNGQVVGNYY644 pKa = 10.39GNLSYY649 pKa = 11.58LNFTDD654 pKa = 3.72TTGSGQVLFEE664 pKa = 4.12QVVSDD669 pKa = 3.62GTYY672 pKa = 10.28DD673 pKa = 4.07AVIATQTAGRR683 pKa = 11.84NVHH686 pKa = 6.51FATDD690 pKa = 4.23AIMGNNNILGEE701 pKa = 4.47AIDD704 pKa = 5.3WVLQDD709 pKa = 3.75NAPDD713 pKa = 3.31ISLLMTRR720 pKa = 11.84NSSIFYY726 pKa = 10.21SRR728 pKa = 11.84NDD730 pKa = 3.06MDD732 pKa = 4.2QSQEE736 pKa = 3.69TWDD739 pKa = 3.79VSGQTPGIYY748 pKa = 10.2DD749 pKa = 3.44AMLPIIEE756 pKa = 4.44TWYY759 pKa = 9.9EE760 pKa = 3.59NYY762 pKa = 10.73GFVGSYY768 pKa = 9.06YY769 pKa = 11.14VNIGSNPPDD778 pKa = 3.53QQTDD782 pKa = 3.02WSVSKK787 pKa = 10.21PYY789 pKa = 10.59YY790 pKa = 10.31DD791 pKa = 3.71RR792 pKa = 11.84LLALEE797 pKa = 4.52SEE799 pKa = 4.8IGTHH803 pKa = 6.29SYY805 pKa = 7.94THH807 pKa = 6.92AVDD810 pKa = 3.73TNLLFPDD817 pKa = 3.96VVTDD821 pKa = 3.66EE822 pKa = 4.77LLQARR827 pKa = 11.84NFVYY831 pKa = 10.63PDD833 pKa = 3.13MTASEE838 pKa = 4.58FNAILADD845 pKa = 3.66TLARR849 pKa = 11.84TDD851 pKa = 3.59PTNPNAIDD859 pKa = 3.91PADD862 pKa = 3.58LTVQEE867 pKa = 4.58KK868 pKa = 10.55AVLEE872 pKa = 3.89ASYY875 pKa = 10.7RR876 pKa = 11.84FQFEE880 pKa = 3.87YY881 pKa = 10.69SKK883 pKa = 11.19LVIEE887 pKa = 4.74RR888 pKa = 11.84EE889 pKa = 3.75MGIQVTGAAVSGAPEE904 pKa = 4.93RR905 pKa = 11.84IDD907 pKa = 3.16ATRR910 pKa = 11.84EE911 pKa = 3.54IIQFFDD917 pKa = 4.39YY918 pKa = 10.8ISGGYY923 pKa = 9.36SGTGAGYY930 pKa = 9.39PGAFGYY936 pKa = 7.39LTPGEE941 pKa = 4.08NDD943 pKa = 3.14RR944 pKa = 11.84VYY946 pKa = 10.8LAPNMSFDD954 pKa = 3.77FSLVGFNGMTPEE966 pKa = 3.88QAEE969 pKa = 4.44ATWLAEE975 pKa = 4.17FAALTANGTTPILAFPWHH993 pKa = 7.57DD994 pKa = 3.74YY995 pKa = 11.49GPTEE999 pKa = 4.38WNLGTPGQVYY1009 pKa = 7.77TFQMYY1014 pKa = 7.68EE1015 pKa = 3.99TLIAAAAASGAEE1027 pKa = 4.16FVTGKK1032 pKa = 10.49DD1033 pKa = 2.97LAEE1036 pKa = 4.95RR1037 pKa = 11.84IASFSASSLTTSRR1050 pKa = 11.84VGDD1053 pKa = 3.71VVNVTVGSSDD1063 pKa = 3.1AGKK1066 pKa = 9.77FAIDD1070 pKa = 4.05LGKK1073 pKa = 10.2EE1074 pKa = 3.98GQIASVTNWYY1084 pKa = 9.98AWDD1087 pKa = 3.75SDD1089 pKa = 4.52SVFLPSSGGTFEE1101 pKa = 4.2ITLGTEE1107 pKa = 3.77MADD1110 pKa = 3.36VTRR1113 pKa = 11.84ISALPMRR1120 pKa = 11.84GDD1122 pKa = 3.81LLSVSGDD1129 pKa = 3.27GSDD1132 pKa = 4.76LDD1134 pKa = 4.28FSFSGRR1140 pKa = 11.84GEE1142 pKa = 4.02VLVTLRR1148 pKa = 11.84SQDD1151 pKa = 3.39SAPIRR1156 pKa = 11.84ITGADD1161 pKa = 3.6SATLPDD1167 pKa = 4.45AGSVSLTFDD1176 pKa = 3.34TVGLNTASVDD1186 pKa = 3.58YY1187 pKa = 10.12LAPGTLLIGDD1197 pKa = 4.69AGSDD1201 pKa = 3.14ILLGGSAADD1210 pKa = 3.64TLEE1213 pKa = 4.0GRR1215 pKa = 11.84GGNDD1219 pKa = 2.68ILSGGGGDD1227 pKa = 3.85DD1228 pKa = 3.35YY1229 pKa = 11.66FVFRR1233 pKa = 11.84LGDD1236 pKa = 3.65AFDD1239 pKa = 4.91TILDD1243 pKa = 4.04FATGIDD1249 pKa = 3.74TLEE1252 pKa = 3.95LSGFGFADD1260 pKa = 4.71AEE1262 pKa = 4.39AAWGAFVDD1270 pKa = 4.0TAEE1273 pKa = 4.24GLEE1276 pKa = 4.23LTFGDD1281 pKa = 4.01TDD1283 pKa = 3.82RR1284 pKa = 11.84LLLAGLNQGDD1294 pKa = 4.75LSSADD1299 pKa = 3.05IVTDD1303 pKa = 3.85PFLFAA1308 pKa = 5.86

Molecular weight: 138.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H7JJ68|A0A1H7JJ68_9RHOB Uncharacterized membrane protein YeiH OS=Jannaschia helgolandensis OX=188906 GN=SAMN04488526_1388 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.08AGRR28 pKa = 11.84RR29 pKa = 11.84ILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.97VLSAA44 pKa = 4.11

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3471

0

3471

1060539

38

5064

305.5

33.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.394 ± 0.057

0.844 ± 0.014

6.559 ± 0.04

5.38 ± 0.038

3.569 ± 0.027

8.851 ± 0.042

1.962 ± 0.022

5.252 ± 0.032

2.765 ± 0.028

10.061 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.836 ± 0.022

2.489 ± 0.026

5.136 ± 0.039

2.996 ± 0.022

7.043 ± 0.039

5.083 ± 0.03

5.903 ± 0.033

7.4 ± 0.033

1.427 ± 0.019

2.049 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski