Ancrocorticia populi
Average proteome isoelectric point is 5.74
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2288 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2V1K690|A0A2V1K690_9ACTO HTH luxR-type domain-containing protein OS=Ancrocorticia populi OX=2175228 GN=DD236_08590 PE=4 SV=1
MM1 pKa = 7.56 RR2 pKa = 11.84 QNKK5 pKa = 9.87 YY6 pKa = 10.32 GVPAMAAAAALMLSACSSSGDD27 pKa = 3.92 DD28 pKa = 3.82 EE29 pKa = 5.84 SSGGDD34 pKa = 3.18 GGSGDD39 pKa = 4.18 KK40 pKa = 10.14 LTVGIKK46 pKa = 10.01 FDD48 pKa = 3.76 QPGLGLQKK56 pKa = 10.52 GSEE59 pKa = 4.17 YY60 pKa = 10.94 SGFDD64 pKa = 2.96 VDD66 pKa = 3.54 VAYY69 pKa = 10.75 YY70 pKa = 10.51 VADD73 pKa = 3.62 KK74 pKa = 11.01 LGYY77 pKa = 10.05 SQDD80 pKa = 3.19 QVEE83 pKa = 4.93 FVQSVTAQRR92 pKa = 11.84 EE93 pKa = 4.28 TMLEE97 pKa = 3.79 NGQADD102 pKa = 4.55 MIVATYY108 pKa = 10.02 TMSDD112 pKa = 3.12 ARR114 pKa = 11.84 KK115 pKa = 8.58 EE116 pKa = 4.21 QIDD119 pKa = 3.55 FAGPYY124 pKa = 9.62 LVAGQSLLVAADD136 pKa = 4.32 DD137 pKa = 4.37 NSISGPDD144 pKa = 3.43 NLDD147 pKa = 3.29 GKK149 pKa = 10.03 KK150 pKa = 10.08 LCSVSGAVPAQRR162 pKa = 11.84 IKK164 pKa = 11.16 DD165 pKa = 3.73 EE166 pKa = 4.27 YY167 pKa = 11.33 ASDD170 pKa = 3.72 VQLYY174 pKa = 7.62 EE175 pKa = 3.68 ASTYY179 pKa = 9.48 SEE181 pKa = 4.23 CVEE184 pKa = 4.0 LLGNGTIDD192 pKa = 4.97 AVTTDD197 pKa = 3.76 DD198 pKa = 5.67 SILAGYY204 pKa = 9.43 AAVPEE209 pKa = 4.04 YY210 pKa = 10.56 DD211 pKa = 3.6 GKK213 pKa = 11.14 FKK215 pKa = 11.12 LVGEE219 pKa = 4.56 AFSEE223 pKa = 4.42 EE224 pKa = 4.66 PYY226 pKa = 11.11 GVGFPKK232 pKa = 10.71 GSDD235 pKa = 2.83 KK236 pKa = 11.36 CEE238 pKa = 3.99 PANEE242 pKa = 4.49 ALTEE246 pKa = 4.2 MIDD249 pKa = 4.35 DD250 pKa = 4.64 GSWDD254 pKa = 3.95 QFLQDD259 pKa = 4.37 NLGDD263 pKa = 4.22 AYY265 pKa = 10.11 TPDD268 pKa = 3.7 ASLNPPEE275 pKa = 4.66 VGGACSS281 pKa = 3.11
Molecular weight: 29.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.692
IPC2_protein 3.795
IPC_protein 3.808
Toseland 3.592
ProMoST 3.973
Dawson 3.795
Bjellqvist 3.961
Wikipedia 3.732
Rodwell 3.63
Grimsley 3.49
Solomon 3.795
Lehninger 3.757
Nozaki 3.91
DTASelect 4.151
Thurlkill 3.643
EMBOSS 3.745
Sillero 3.935
Patrickios 1.227
IPC_peptide 3.795
IPC2_peptide 3.91
IPC2.peptide.svr19 3.82
Protein with the highest isoelectric point:
>tr|A0A2V1K631|A0A2V1K631_9ACTO GntR family transcriptional regulator OS=Ancrocorticia populi OX=2175228 GN=DD236_11495 PE=4 SV=1
MM1 pKa = 7.41 VKK3 pKa = 9.03 RR4 pKa = 11.84 TFQPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 AKK15 pKa = 9.4 VHH17 pKa = 5.57 GFRR20 pKa = 11.84 KK21 pKa = 10.13 RR22 pKa = 11.84 MGTRR26 pKa = 11.84 AGRR29 pKa = 11.84 AVLANRR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 KK38 pKa = 9.43 GRR40 pKa = 11.84 HH41 pKa = 4.95 SLSAA45 pKa = 3.67
Molecular weight: 5.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.676
Grimsley 13.086
Solomon 13.539
Lehninger 13.437
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.398
IPC_peptide 13.539
IPC2_peptide 12.53
IPC2.peptide.svr19 9.215
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2288
0
2288
792421
29
2979
346.3
37.44
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.32 ± 0.055
0.752 ± 0.014
6.043 ± 0.047
6.531 ± 0.047
3.214 ± 0.031
8.697 ± 0.049
1.981 ± 0.022
5.301 ± 0.039
2.958 ± 0.034
9.613 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.286 ± 0.022
2.76 ± 0.029
4.913 ± 0.035
3.303 ± 0.033
6.069 ± 0.056
6.401 ± 0.036
5.946 ± 0.044
8.149 ± 0.045
1.415 ± 0.021
2.346 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here