Flavobacterium sp. LLJ-11

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Flavobacterium; unclassified Flavobacterium

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2377 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q1K6Z3|A0A4Q1K6Z3_9FLAO MotA/TolQ/ExbB proton channel family protein OS=Flavobacterium sp. LLJ-11 OX=2506422 GN=EQG63_05370 PE=3 SV=1
MM1 pKa = 7.24AQDD4 pKa = 3.28ITIKK8 pKa = 9.4ITDD11 pKa = 3.82RR12 pKa = 11.84EE13 pKa = 4.65GVTHH17 pKa = 6.71EE18 pKa = 4.31VQAPTDD24 pKa = 3.44MNMNIMEE31 pKa = 4.73LVRR34 pKa = 11.84AYY36 pKa = 9.97EE37 pKa = 4.0LAPEE41 pKa = 4.26GTIGICGGMAMCASCQCYY59 pKa = 10.15VLNDD63 pKa = 3.31VTLPEE68 pKa = 4.21MGQDD72 pKa = 3.43EE73 pKa = 4.38EE74 pKa = 5.01AMLWEE79 pKa = 4.46AFNVKK84 pKa = 10.27DD85 pKa = 4.32NSRR88 pKa = 11.84LGCQIHH94 pKa = 6.11ITPDD98 pKa = 2.95IDD100 pKa = 3.59GLEE103 pKa = 4.13VEE105 pKa = 5.29LAPEE109 pKa = 3.92AA110 pKa = 4.19

Molecular weight: 12.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4V1N263|A0A4V1N263_9FLAO SCO family protein OS=Flavobacterium sp. LLJ-11 OX=2506422 GN=EQG63_01430 PE=3 SV=1
MM1 pKa = 7.4GVIHH5 pKa = 7.18KK6 pKa = 10.15IGRR9 pKa = 11.84RR10 pKa = 11.84KK11 pKa = 9.62CAVARR16 pKa = 11.84VYY18 pKa = 10.92VSEE21 pKa = 4.22GTGTITVNKK30 pKa = 9.55RR31 pKa = 11.84AFATYY36 pKa = 10.1FPTATLQYY44 pKa = 10.79KK45 pKa = 9.22VLQPMSMTEE54 pKa = 3.41NATNYY59 pKa = 9.6DD60 pKa = 3.25VKK62 pKa = 11.0INVYY66 pKa = 10.41GGGATGQAEE75 pKa = 4.67AVRR78 pKa = 11.84MAIARR83 pKa = 11.84AMCEE87 pKa = 3.76VGEE90 pKa = 4.27GNRR93 pKa = 11.84AILKK97 pKa = 10.23PEE99 pKa = 4.1GLLTRR104 pKa = 11.84DD105 pKa = 3.08PRR107 pKa = 11.84MVEE110 pKa = 3.8RR111 pKa = 11.84KK112 pKa = 10.02KK113 pKa = 10.83FGQKK117 pKa = 9.4KK118 pKa = 8.61ARR120 pKa = 11.84KK121 pKa = 8.51RR122 pKa = 11.84FQFSKK127 pKa = 10.78RR128 pKa = 3.42

Molecular weight: 14.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2377

0

2377

813841

38

3854

342.4

38.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.585 ± 0.059

0.789 ± 0.017

5.146 ± 0.036

6.441 ± 0.062

5.402 ± 0.041

6.383 ± 0.055

1.704 ± 0.026

7.989 ± 0.051

7.957 ± 0.07

8.928 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.239 ± 0.027

6.464 ± 0.061

3.373 ± 0.038

3.497 ± 0.028

3.077 ± 0.032

6.433 ± 0.04

6.194 ± 0.079

6.399 ± 0.047

0.966 ± 0.017

4.032 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski