Amino acid dipepetide frequency for Flavobacterium sp. LLJ-11

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.366AlaAla: 4.366 ± 0.122
0.565AlaCys: 0.565 ± 0.03
3.224AlaAsp: 3.224 ± 0.08
4.02AlaGlu: 4.02 ± 0.085
3.348AlaPhe: 3.348 ± 0.061
4.431AlaGly: 4.431 ± 0.101
1.094AlaHis: 1.094 ± 0.042
5.578AlaIle: 5.578 ± 0.093
5.207AlaLys: 5.207 ± 0.109
6.004AlaLeu: 6.004 ± 0.1
1.556AlaMet: 1.556 ± 0.046
3.974AlaAsn: 3.974 ± 0.091
2.034AlaPro: 2.034 ± 0.068
2.671AlaGln: 2.671 ± 0.062
1.842AlaArg: 1.842 ± 0.055
4.371AlaSer: 4.371 ± 0.095
4.525AlaThr: 4.525 ± 0.129
4.179AlaVal: 4.179 ± 0.079
0.57AlaTrp: 0.57 ± 0.027
2.294AlaTyr: 2.294 ± 0.056
0.0AlaXaa: 0.0 ± 0.0
Cys
0.52CysAla: 0.52 ± 0.032
0.092CysCys: 0.092 ± 0.011
0.468CysAsp: 0.468 ± 0.026
0.464CysGlu: 0.464 ± 0.026
0.426CysPhe: 0.426 ± 0.022
0.677CysGly: 0.677 ± 0.037
0.176CysHis: 0.176 ± 0.017
0.581CysIle: 0.581 ± 0.031
0.457CysLys: 0.457 ± 0.025
0.654CysLeu: 0.654 ± 0.027
0.155CysMet: 0.155 ± 0.014
0.496CysAsn: 0.496 ± 0.028
0.364CysPro: 0.364 ± 0.023
0.265CysGln: 0.265 ± 0.017
0.177CysArg: 0.177 ± 0.016
0.589CysSer: 0.589 ± 0.03
0.501CysThr: 0.501 ± 0.035
0.448CysVal: 0.448 ± 0.026
0.061CysTrp: 0.061 ± 0.009
0.316CysTyr: 0.316 ± 0.018
0.0CysXaa: 0.0 ± 0.0
Asp
3.689AspAla: 3.689 ± 0.073
0.457AspCys: 0.457 ± 0.025
2.467AspAsp: 2.467 ± 0.061
3.364AspGlu: 3.364 ± 0.083
3.573AspPhe: 3.573 ± 0.069
3.418AspGly: 3.418 ± 0.076
0.707AspHis: 0.707 ± 0.032
3.966AspIle: 3.966 ± 0.073
4.223AspLys: 4.223 ± 0.083
4.858AspLeu: 4.858 ± 0.098
1.014AspMet: 1.014 ± 0.03
2.999AspAsn: 2.999 ± 0.068
1.459AspPro: 1.459 ± 0.052
1.273AspGln: 1.273 ± 0.042
1.631AspArg: 1.631 ± 0.048
2.999AspSer: 2.999 ± 0.064
2.639AspThr: 2.639 ± 0.058
3.432AspVal: 3.432 ± 0.07
0.643AspTrp: 0.643 ± 0.028
2.653AspTyr: 2.653 ± 0.057
0.0AspXaa: 0.0 ± 0.0
Glu
4.414GluAla: 4.414 ± 0.103
0.375GluCys: 0.375 ± 0.019
3.06GluAsp: 3.06 ± 0.07
4.722GluGlu: 4.722 ± 0.112
2.966GluPhe: 2.966 ± 0.07
3.499GluGly: 3.499 ± 0.069
1.027GluHis: 1.027 ± 0.037
5.855GluIle: 5.855 ± 0.109
6.507GluLys: 6.507 ± 0.118
5.694GluLeu: 5.694 ± 0.101
1.729GluMet: 1.729 ± 0.049
4.924GluAsn: 4.924 ± 0.094
1.4GluPro: 1.4 ± 0.045
2.096GluGln: 2.096 ± 0.054
2.23GluArg: 2.23 ± 0.064
3.164GluSer: 3.164 ± 0.057
3.64GluThr: 3.64 ± 0.064
4.313GluVal: 4.313 ± 0.086
0.522GluTrp: 0.522 ± 0.027
2.276GluTyr: 2.276 ± 0.047
0.0GluXaa: 0.0 ± 0.0
Phe
3.299PheAla: 3.299 ± 0.066
0.507PheCys: 0.507 ± 0.027
3.268PheAsp: 3.268 ± 0.061
3.642PheGlu: 3.642 ± 0.077
2.883PhePhe: 2.883 ± 0.068
3.852PheGly: 3.852 ± 0.082
0.897PheHis: 0.897 ± 0.035
3.766PheIle: 3.766 ± 0.075
3.703PheLys: 3.703 ± 0.084
4.798PheLeu: 4.798 ± 0.095
1.186PheMet: 1.186 ± 0.041
3.298PheAsn: 3.298 ± 0.072
1.782PhePro: 1.782 ± 0.048
1.784PheGln: 1.784 ± 0.048
1.515PheArg: 1.515 ± 0.046
4.161PheSer: 4.161 ± 0.081
3.406PheThr: 3.406 ± 0.082
3.439PheVal: 3.439 ± 0.063
0.55PheTrp: 0.55 ± 0.029
2.287PheTyr: 2.287 ± 0.062
0.0PheXaa: 0.0 ± 0.0
Gly
4.294GlyAla: 4.294 ± 0.11
0.675GlyCys: 0.675 ± 0.039
3.044GlyAsp: 3.044 ± 0.063
3.316GlyGlu: 3.316 ± 0.057
3.678GlyPhe: 3.678 ± 0.078
4.641GlyGly: 4.641 ± 0.121
1.132GlyHis: 1.132 ± 0.032
5.574GlyIle: 5.574 ± 0.088
5.118GlyLys: 5.118 ± 0.092
5.501GlyLeu: 5.501 ± 0.084
1.624GlyMet: 1.624 ± 0.051
3.939GlyAsn: 3.939 ± 0.09
1.326GlyPro: 1.326 ± 0.048
1.962GlyGln: 1.962 ± 0.05
1.911GlyArg: 1.911 ± 0.059
3.974GlySer: 3.974 ± 0.098
4.485GlyThr: 4.485 ± 0.152
4.249GlyVal: 4.249 ± 0.073
0.686GlyTrp: 0.686 ± 0.029
2.7GlyTyr: 2.7 ± 0.06
0.0GlyXaa: 0.0 ± 0.0
His
0.979HisAla: 0.979 ± 0.034
0.177HisCys: 0.177 ± 0.015
0.795HisAsp: 0.795 ± 0.035
0.988HisGlu: 0.988 ± 0.041
1.216HisPhe: 1.216 ± 0.043
0.962HisGly: 0.962 ± 0.035
0.471HisHis: 0.471 ± 0.029
1.316HisIle: 1.316 ± 0.044
1.169HisLys: 1.169 ± 0.045
1.691HisLeu: 1.691 ± 0.055
0.328HisMet: 0.328 ± 0.022
0.998HisAsn: 0.998 ± 0.035
0.773HisPro: 0.773 ± 0.034
0.779HisGln: 0.779 ± 0.034
0.586HisArg: 0.586 ± 0.028
1.031HisSer: 1.031 ± 0.035
0.901HisThr: 0.901 ± 0.036
0.88HisVal: 0.88 ± 0.033
0.163HisTrp: 0.163 ± 0.014
0.838HisTyr: 0.838 ± 0.029
0.0HisXaa: 0.0 ± 0.0
Ile
5.942IleAla: 5.942 ± 0.096
0.655IleCys: 0.655 ± 0.03
4.729IleAsp: 4.729 ± 0.089
5.44IleGlu: 5.44 ± 0.096
3.766IlePhe: 3.766 ± 0.082
5.082IleGly: 5.082 ± 0.084
1.366IleHis: 1.366 ± 0.046
6.092IleIle: 6.092 ± 0.106
5.841IleLys: 5.841 ± 0.1
6.778IleLeu: 6.778 ± 0.104
1.455IleMet: 1.455 ± 0.043
4.847IleAsn: 4.847 ± 0.096
3.222IlePro: 3.222 ± 0.073
2.902IleGln: 2.902 ± 0.067
2.39IleArg: 2.39 ± 0.06
5.711IleSer: 5.711 ± 0.086
5.123IleThr: 5.123 ± 0.102
5.112IleVal: 5.112 ± 0.085
0.67IleTrp: 0.67 ± 0.026
2.768IleTyr: 2.768 ± 0.065
0.0IleXaa: 0.0 ± 0.0
Lys
5.32LysAla: 5.32 ± 0.13
0.387LysCys: 0.387 ± 0.023
4.017LysAsp: 4.017 ± 0.087
6.021LysGlu: 6.021 ± 0.113
3.246LysPhe: 3.246 ± 0.07
4.405LysGly: 4.405 ± 0.096
1.273LysHis: 1.273 ± 0.042
6.91LysIle: 6.91 ± 0.116
7.234LysLys: 7.234 ± 0.158
6.676LysLeu: 6.676 ± 0.104
2.358LysMet: 2.358 ± 0.06
5.653LysAsn: 5.653 ± 0.097
2.508LysPro: 2.508 ± 0.067
2.751LysGln: 2.751 ± 0.065
2.548LysArg: 2.548 ± 0.063
4.637LysSer: 4.637 ± 0.08
4.68LysThr: 4.68 ± 0.079
5.01LysVal: 5.01 ± 0.1
0.774LysTrp: 0.774 ± 0.031
3.164LysTyr: 3.164 ± 0.075
0.0LysXaa: 0.0 ± 0.0
Leu
5.809LeuAla: 5.809 ± 0.092
0.646LeuCys: 0.646 ± 0.028
4.619LeuAsp: 4.619 ± 0.085
5.845LeuGlu: 5.845 ± 0.106
5.03LeuPhe: 5.03 ± 0.11
5.722LeuGly: 5.722 ± 0.095
1.597LeuHis: 1.597 ± 0.047
6.445LeuIle: 6.445 ± 0.116
7.326LeuLys: 7.326 ± 0.138
8.551LeuLeu: 8.551 ± 0.143
1.976LeuMet: 1.976 ± 0.046
5.477LeuAsn: 5.477 ± 0.089
3.455LeuPro: 3.455 ± 0.066
3.314LeuGln: 3.314 ± 0.071
2.786LeuArg: 2.786 ± 0.068
6.273LeuSer: 6.273 ± 0.105
5.091LeuThr: 5.091 ± 0.079
5.465LeuVal: 5.465 ± 0.084
0.727LeuTrp: 0.727 ± 0.031
3.125LeuTyr: 3.125 ± 0.077
0.0LeuXaa: 0.0 ± 0.0
Met
1.604MetAla: 1.604 ± 0.05
0.15MetCys: 0.15 ± 0.014
1.038MetAsp: 1.038 ± 0.038
1.412MetGlu: 1.412 ± 0.048
0.909MetPhe: 0.909 ± 0.035
1.431MetGly: 1.431 ± 0.05
0.393MetHis: 0.393 ± 0.022
1.628MetIle: 1.628 ± 0.048
2.324MetLys: 2.324 ± 0.061
1.869MetLeu: 1.869 ± 0.054
0.602MetMet: 0.602 ± 0.03
1.451MetAsn: 1.451 ± 0.04
0.85MetPro: 0.85 ± 0.029
0.883MetGln: 0.883 ± 0.034
0.899MetArg: 0.899 ± 0.033
1.449MetSer: 1.449 ± 0.049
1.214MetThr: 1.214 ± 0.035
1.306MetVal: 1.306 ± 0.042
0.166MetTrp: 0.166 ± 0.013
0.809MetTyr: 0.809 ± 0.035
0.0MetXaa: 0.0 ± 0.0
Asn
4.159AsnAla: 4.159 ± 0.078
0.526AsnCys: 0.526 ± 0.028
3.203AsnAsp: 3.203 ± 0.074
3.92AsnGlu: 3.92 ± 0.072
3.377AsnPhe: 3.377 ± 0.075
4.409AsnGly: 4.409 ± 0.12
1.107AsnHis: 1.107 ± 0.038
4.787AsnIle: 4.787 ± 0.079
4.564AsnLys: 4.564 ± 0.092
5.494AsnLeu: 5.494 ± 0.105
1.268AsnMet: 1.268 ± 0.046
4.213AsnAsn: 4.213 ± 0.099
3.071AsnPro: 3.071 ± 0.068
2.589AsnGln: 2.589 ± 0.051
2.041AsnArg: 2.041 ± 0.053
4.038AsnSer: 4.038 ± 0.088
4.011AsnThr: 4.011 ± 0.097
3.927AsnVal: 3.927 ± 0.087
0.78AsnTrp: 0.78 ± 0.033
3.158AsnTyr: 3.158 ± 0.079
0.0AsnXaa: 0.0 ± 0.0
Pro
2.124ProAla: 2.124 ± 0.068
0.23ProCys: 0.23 ± 0.018
1.707ProAsp: 1.707 ± 0.045
2.62ProGlu: 2.62 ± 0.068
1.95ProPhe: 1.95 ± 0.055
1.898ProGly: 1.898 ± 0.074
0.562ProHis: 0.562 ± 0.03
2.803ProIle: 2.803 ± 0.064
2.508ProLys: 2.508 ± 0.072
2.761ProLeu: 2.761 ± 0.064
0.715ProMet: 0.715 ± 0.032
2.528ProAsn: 2.528 ± 0.069
0.748ProPro: 0.748 ± 0.042
1.078ProGln: 1.078 ± 0.036
0.876ProArg: 0.876 ± 0.038
2.217ProSer: 2.217 ± 0.059
2.403ProThr: 2.403 ± 0.08
2.406ProVal: 2.406 ± 0.056
0.285ProTrp: 0.285 ± 0.019
1.316ProTyr: 1.316 ± 0.044
0.0ProXaa: 0.0 ± 0.0
Gln
2.023GlnAla: 2.023 ± 0.05
0.209GlnCys: 0.209 ± 0.019
1.607GlnAsp: 1.607 ± 0.043
2.351GlnGlu: 2.351 ± 0.06
1.994GlnPhe: 1.994 ± 0.048
1.649GlnGly: 1.649 ± 0.046
0.639GlnHis: 0.639 ± 0.027
2.917GlnIle: 2.917 ± 0.065
3.249GlnLys: 3.249 ± 0.078
3.48GlnLeu: 3.48 ± 0.06
0.856GlnMet: 0.856 ± 0.032
2.531GlnAsn: 2.531 ± 0.06
1.091GlnPro: 1.091 ± 0.039
1.468GlnGln: 1.468 ± 0.047
1.087GlnArg: 1.087 ± 0.038
2.004GlnSer: 2.004 ± 0.049
2.054GlnThr: 2.054 ± 0.055
2.051GlnVal: 2.051 ± 0.048
0.342GlnTrp: 0.342 ± 0.021
1.371GlnTyr: 1.371 ± 0.042
0.0GlnXaa: 0.0 ± 0.0
Arg
1.881ArgAla: 1.881 ± 0.058
0.183ArgCys: 0.183 ± 0.014
1.561ArgAsp: 1.561 ± 0.042
1.987ArgGlu: 1.987 ± 0.055
1.779ArgPhe: 1.779 ± 0.051
1.761ArgGly: 1.761 ± 0.052
0.535ArgHis: 0.535 ± 0.026
2.673ArgIle: 2.673 ± 0.054
2.606ArgLys: 2.606 ± 0.063
2.878ArgLeu: 2.878 ± 0.072
0.879ArgMet: 0.879 ± 0.035
2.036ArgAsn: 2.036 ± 0.049
0.993ArgPro: 0.993 ± 0.041
0.973ArgGln: 0.973 ± 0.037
1.139ArgArg: 1.139 ± 0.04
1.606ArgSer: 1.606 ± 0.048
1.699ArgThr: 1.699 ± 0.043
2.009ArgVal: 2.009 ± 0.048
0.308ArgTrp: 0.308 ± 0.021
1.284ArgTyr: 1.284 ± 0.045
0.0ArgXaa: 0.0 ± 0.0
Ser
3.885SerAla: 3.885 ± 0.074
0.629SerCys: 0.629 ± 0.031
3.427SerAsp: 3.427 ± 0.067
3.943SerGlu: 3.943 ± 0.081
3.828SerPhe: 3.828 ± 0.077
4.645SerGly: 4.645 ± 0.113
1.118SerHis: 1.118 ± 0.041
5.137SerIle: 5.137 ± 0.084
4.947SerLys: 4.947 ± 0.083
5.581SerLeu: 5.581 ± 0.089
1.267SerMet: 1.267 ± 0.039
4.081SerAsn: 4.081 ± 0.102
2.101SerPro: 2.101 ± 0.059
2.289SerGln: 2.289 ± 0.059
1.871SerArg: 1.871 ± 0.047
4.145SerSer: 4.145 ± 0.107
3.91SerThr: 3.91 ± 0.109
4.265SerVal: 4.265 ± 0.077
0.672SerTrp: 0.672 ± 0.032
2.589SerTyr: 2.589 ± 0.064
0.0SerXaa: 0.0 ± 0.0
Thr
4.113ThrAla: 4.113 ± 0.138
0.462ThrCys: 0.462 ± 0.029
3.024ThrAsp: 3.024 ± 0.06
3.474ThrGlu: 3.474 ± 0.081
3.395ThrPhe: 3.395 ± 0.077
4.312ThrGly: 4.312 ± 0.127
0.974ThrHis: 0.974 ± 0.034
5.416ThrIle: 5.416 ± 0.108
4.179ThrLys: 4.179 ± 0.082
5.446ThrLeu: 5.446 ± 0.09
1.011ThrMet: 1.011 ± 0.036
3.926ThrAsn: 3.926 ± 0.117
2.755ThrPro: 2.755 ± 0.089
2.078ThrGln: 2.078 ± 0.054
1.559ThrArg: 1.559 ± 0.041
4.28ThrSer: 4.28 ± 0.099
4.433ThrThr: 4.433 ± 0.154
4.061ThrVal: 4.061 ± 0.116
0.537ThrTrp: 0.537 ± 0.028
2.509ThrTyr: 2.509 ± 0.093
0.0ThrXaa: 0.0 ± 0.0
Val
4.448ValAla: 4.448 ± 0.08
0.543ValCys: 0.543 ± 0.027
3.443ValAsp: 3.443 ± 0.066
4.007ValGlu: 4.007 ± 0.083
3.509ValPhe: 3.509 ± 0.074
3.941ValGly: 3.941 ± 0.074
0.997ValHis: 0.997 ± 0.037
4.97ValIle: 4.97 ± 0.08
4.674ValLys: 4.674 ± 0.091
6.056ValLeu: 6.056 ± 0.103
1.39ValMet: 1.39 ± 0.047
3.793ValAsn: 3.793 ± 0.089
2.181ValPro: 2.181 ± 0.057
1.886ValGln: 1.886 ± 0.044
1.962ValArg: 1.962 ± 0.057
4.392ValSer: 4.392 ± 0.075
4.083ValThr: 4.083 ± 0.126
4.506ValVal: 4.506 ± 0.088
0.618ValTrp: 0.618 ± 0.027
2.589ValTyr: 2.589 ± 0.06
0.0ValXaa: 0.0 ± 0.0
Trp
0.596TrpAla: 0.596 ± 0.031
0.103TrpCys: 0.103 ± 0.011
0.485TrpAsp: 0.485 ± 0.026
0.598TrpGlu: 0.598 ± 0.027
0.578TrpPhe: 0.578 ± 0.03
0.578TrpGly: 0.578 ± 0.029
0.194TrpHis: 0.194 ± 0.015
0.7TrpIle: 0.7 ± 0.03
0.813TrpLys: 0.813 ± 0.034
0.837TrpLeu: 0.837 ± 0.037
0.278TrpMet: 0.278 ± 0.018
0.726TrpAsn: 0.726 ± 0.03
0.178TrpPro: 0.178 ± 0.017
0.374TrpGln: 0.374 ± 0.022
0.338TrpArg: 0.338 ± 0.021
0.584TrpSer: 0.584 ± 0.031
0.575TrpThr: 0.575 ± 0.03
0.581TrpVal: 0.581 ± 0.026
0.131TrpTrp: 0.131 ± 0.014
0.408TrpTyr: 0.408 ± 0.024
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.387TyrAla: 2.387 ± 0.051
0.317TyrCys: 0.317 ± 0.019
2.278TyrAsp: 2.278 ± 0.068
2.278TyrGlu: 2.278 ± 0.056
2.572TyrPhe: 2.572 ± 0.066
2.514TyrGly: 2.514 ± 0.059
0.783TyrHis: 0.783 ± 0.038
2.776TyrIle: 2.776 ± 0.063
2.924TyrLys: 2.924 ± 0.072
3.674TyrLeu: 3.674 ± 0.073
0.741TyrMet: 0.741 ± 0.033
2.75TyrAsn: 2.75 ± 0.076
1.445TyrPro: 1.445 ± 0.049
1.557TyrGln: 1.557 ± 0.047
1.38TyrArg: 1.38 ± 0.042
2.709TyrSer: 2.709 ± 0.069
2.569TyrThr: 2.569 ± 0.087
2.349TyrVal: 2.349 ± 0.054
0.448TyrTrp: 0.448 ± 0.028
1.873TyrTyr: 1.873 ± 0.06
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2377 proteins (813841 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski