Candidatus Nitrosocosmicus sp. SS

Taxonomy: cellular organisms; Archaea; TACK group; Thaumarchaeota; Nitrososphaeria; Nitrososphaerales; Nitrososphaeraceae; Candidatus Nitrosocosmicus; unclassified Candidatus Nitrosocosmicus

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2921 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5B2Z3Z0|A0A5B2Z3Z0_9ARCH DoxX family protein OS=Candidatus Nitrosocosmicus sp. SS OX=2563600 GN=F1Z66_06950 PE=4 SV=1
MM1 pKa = 7.53TKK3 pKa = 9.86PLPIAFMITNILVLLLFSNVFLNHH27 pKa = 5.1NWAVYY32 pKa = 10.5AGGDD36 pKa = 3.68GDD38 pKa = 4.05GEE40 pKa = 4.9RR41 pKa = 11.84YY42 pKa = 8.01TGPLNQGDD50 pKa = 4.3SQEE53 pKa = 3.98KK54 pKa = 9.77MIYY57 pKa = 8.85CTFNQEE63 pKa = 3.75VKK65 pKa = 10.93APLCEE70 pKa = 4.08GTSRR74 pKa = 11.84SDD76 pKa = 3.38IIIGTVQEE84 pKa = 4.74DD85 pKa = 4.66FIKK88 pKa = 11.03GKK90 pKa = 10.84GSDD93 pKa = 3.71DD94 pKa = 3.63ALQGRR99 pKa = 11.84ASADD103 pKa = 3.83DD104 pKa = 3.5IFGGDD109 pKa = 4.14GDD111 pKa = 5.65DD112 pKa = 4.5DD113 pKa = 4.24VQGGEE118 pKa = 4.39GSDD121 pKa = 3.58NIYY124 pKa = 11.24GEE126 pKa = 4.85DD127 pKa = 4.61GNDD130 pKa = 3.5LLYY133 pKa = 11.22GGLDD137 pKa = 3.09GDD139 pKa = 4.25YY140 pKa = 11.27LVGGKK145 pKa = 10.64GNDD148 pKa = 3.24EE149 pKa = 4.57LYY151 pKa = 10.98AGAGEE156 pKa = 4.59DD157 pKa = 4.11LLEE160 pKa = 4.77GGPGSNYY167 pKa = 9.56FDD169 pKa = 5.18CGDD172 pKa = 4.64DD173 pKa = 3.44YY174 pKa = 11.76DD175 pKa = 5.83IIIDD179 pKa = 4.19FDD181 pKa = 3.98PSKK184 pKa = 11.27DD185 pKa = 3.55VASNNCEE192 pKa = 4.35DD193 pKa = 3.99VRR195 pKa = 11.84TDD197 pKa = 3.25LL198 pKa = 5.15

Molecular weight: 21.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5B2Z4D8|A0A5B2Z4D8_9ARCH GTP cyclohydrolase I OS=Candidatus Nitrosocosmicus sp. SS OX=2563600 GN=folE PE=4 SV=1
MM1 pKa = 7.83AARR4 pKa = 11.84KK5 pKa = 8.19STTRR9 pKa = 11.84KK10 pKa = 9.19IRR12 pKa = 11.84LLKK15 pKa = 10.21KK16 pKa = 9.86IKK18 pKa = 9.51QNRR21 pKa = 11.84PVPAWIIIRR30 pKa = 11.84THH32 pKa = 4.68RR33 pKa = 11.84HH34 pKa = 3.48VRR36 pKa = 11.84TNPKK40 pKa = 8.58RR41 pKa = 11.84RR42 pKa = 11.84SWRR45 pKa = 11.84RR46 pKa = 11.84SDD48 pKa = 3.69VNIGG52 pKa = 3.2

Molecular weight: 6.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2921

0

2921

717542

25

1521

245.6

27.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.06 ± 0.046

1.03 ± 0.02

5.748 ± 0.043

6.032 ± 0.039

4.501 ± 0.036

6.143 ± 0.05

1.849 ± 0.021

9.361 ± 0.05

7.557 ± 0.048

9.11 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.357 ± 0.025

6.631 ± 0.056

3.69 ± 0.037

3.135 ± 0.033

3.76 ± 0.035

8.15 ± 0.049

5.449 ± 0.041

6.0 ± 0.034

0.886 ± 0.016

3.553 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski