Taylorella asinigenitalis (strain MCE3)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Alcaligenaceae; Taylorella; Taylorella asinigenitalis

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1523 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G4QDH5|G4QDH5_TAYAM GTPase Obg OS=Taylorella asinigenitalis (strain MCE3) OX=1008459 GN=obg PE=3 SV=1
MM1 pKa = 7.39PQFNEE6 pKa = 4.21LDD8 pKa = 3.78LLIYY12 pKa = 10.38GYY14 pKa = 11.05LNEE17 pKa = 5.35DD18 pKa = 3.45FDD20 pKa = 6.0LICHH24 pKa = 6.45TDD26 pKa = 3.37SLEE29 pKa = 4.03GAIDD33 pKa = 4.06YY34 pKa = 10.13YY35 pKa = 10.49VTDD38 pKa = 3.52VSEE41 pKa = 4.33NTLKK45 pKa = 11.12ALLKK49 pKa = 9.98EE50 pKa = 4.34LNDD53 pKa = 3.96FEE55 pKa = 4.42QTYY58 pKa = 10.45DD59 pKa = 3.31ANRR62 pKa = 11.84DD63 pKa = 3.52EE64 pKa = 4.71EE65 pKa = 4.4FEE67 pKa = 4.26NLFMSGVDD75 pKa = 3.94YY76 pKa = 11.08ISVDD80 pKa = 3.22HH81 pKa = 6.82LLGLIRR87 pKa = 11.84STIKK91 pKa = 10.14KK92 pKa = 9.51HH93 pKa = 5.68YY94 pKa = 8.87PDD96 pKa = 5.52LIEE99 pKa = 4.36GAA101 pKa = 4.07

Molecular weight: 11.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G4QCG5|G4QCG5_TAYAM Uncharacterized protein OS=Taylorella asinigenitalis (strain MCE3) OX=1008459 GN=TASI_0313 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVTRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.43RR14 pKa = 11.84THH16 pKa = 5.88GFLVRR21 pKa = 11.84MKK23 pKa = 9.7TRR25 pKa = 11.84GGRR28 pKa = 11.84AVINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.58GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1523

0

1523

499495

37

3352

328.0

36.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.632 ± 0.067

0.886 ± 0.023

5.59 ± 0.05

6.624 ± 0.055

4.342 ± 0.044

6.879 ± 0.069

1.993 ± 0.031

7.389 ± 0.046

6.736 ± 0.057

9.835 ± 0.073

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.396 ± 0.036

4.875 ± 0.06

4.065 ± 0.041

3.353 ± 0.038

4.498 ± 0.053

6.994 ± 0.051

5.008 ± 0.039

6.664 ± 0.049

1.065 ± 0.021

3.175 ± 0.043
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski