Amino acid dipepetide frequency for Thermoanaerobaculum aquaticum

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
14.263AlaAla: 14.263 ± 0.211
1.392AlaCys: 1.392 ± 0.049
4.215AlaAsp: 4.215 ± 0.081
7.549AlaGlu: 7.549 ± 0.108
4.888AlaPhe: 4.888 ± 0.081
9.583AlaGly: 9.583 ± 0.129
2.222AlaHis: 2.222 ± 0.054
3.627AlaIle: 3.627 ± 0.067
4.003AlaLys: 4.003 ± 0.075
14.381AlaLeu: 14.381 ± 0.203
2.212AlaMet: 2.212 ± 0.064
2.393AlaAsn: 2.393 ± 0.067
5.261AlaPro: 5.261 ± 0.108
5.055AlaGln: 5.055 ± 0.09
8.58AlaArg: 8.58 ± 0.114
6.443AlaSer: 6.443 ± 0.099
5.194AlaThr: 5.194 ± 0.079
9.997AlaVal: 9.997 ± 0.143
1.982AlaTrp: 1.982 ± 0.057
2.402AlaTyr: 2.402 ± 0.063
0.0AlaXaa: 0.0 ± 0.0
Cys
0.826CysAla: 0.826 ± 0.03
0.114CysCys: 0.114 ± 0.014
0.427CysAsp: 0.427 ± 0.025
0.498CysGlu: 0.498 ± 0.027
0.382CysPhe: 0.382 ± 0.021
1.043CysGly: 1.043 ± 0.043
0.517CysHis: 0.517 ± 0.056
0.223CysIle: 0.223 ± 0.018
0.201CysLys: 0.201 ± 0.017
1.053CysLeu: 1.053 ± 0.036
0.122CysMet: 0.122 ± 0.013
0.215CysAsn: 0.215 ± 0.018
0.691CysPro: 0.691 ± 0.033
0.477CysGln: 0.477 ± 0.026
0.736CysArg: 0.736 ± 0.031
0.504CysSer: 0.504 ± 0.028
0.432CysThr: 0.432 ± 0.026
0.595CysVal: 0.595 ± 0.028
0.154CysTrp: 0.154 ± 0.013
0.185CysTyr: 0.185 ± 0.016
0.0CysXaa: 0.0 ± 0.0
Asp
4.013AspAla: 4.013 ± 0.064
0.352AspCys: 0.352 ± 0.022
1.508AspAsp: 1.508 ± 0.047
2.676AspGlu: 2.676 ± 0.065
1.942AspPhe: 1.942 ± 0.061
3.566AspGly: 3.566 ± 0.083
0.861AspHis: 0.861 ± 0.031
1.497AspIle: 1.497 ± 0.053
1.073AspLys: 1.073 ± 0.041
4.864AspLeu: 4.864 ± 0.079
0.553AspMet: 0.553 ± 0.028
0.838AspAsn: 0.838 ± 0.034
3.55AspPro: 3.55 ± 0.067
1.265AspGln: 1.265 ± 0.041
3.057AspArg: 3.057 ± 0.059
1.659AspSer: 1.659 ± 0.049
1.664AspThr: 1.664 ± 0.051
3.418AspVal: 3.418 ± 0.062
0.638AspTrp: 0.638 ± 0.029
0.961AspTyr: 0.961 ± 0.037
0.0AspXaa: 0.0 ± 0.0
Glu
8.729GluAla: 8.729 ± 0.113
0.348GluCys: 0.348 ± 0.024
2.744GluAsp: 2.744 ± 0.06
5.75GluGlu: 5.75 ± 0.123
2.037GluPhe: 2.037 ± 0.05
5.279GluGly: 5.279 ± 0.088
1.266GluHis: 1.266 ± 0.045
2.551GluIle: 2.551 ± 0.061
3.436GluLys: 3.436 ± 0.09
9.279GluLeu: 9.279 ± 0.133
1.062GluMet: 1.062 ± 0.037
1.557GluAsn: 1.557 ± 0.051
3.184GluPro: 3.184 ± 0.068
2.59GluGln: 2.59 ± 0.062
5.296GluArg: 5.296 ± 0.1
2.471GluSer: 2.471 ± 0.061
2.795GluThr: 2.795 ± 0.063
6.707GluVal: 6.707 ± 0.105
0.691GluTrp: 0.691 ± 0.029
1.002GluTyr: 1.002 ± 0.039
0.0GluXaa: 0.0 ± 0.0
Phe
4.732PheAla: 4.732 ± 0.085
0.504PheCys: 0.504 ± 0.026
1.838PheAsp: 1.838 ± 0.053
1.901PheGlu: 1.901 ± 0.047
2.12PhePhe: 2.12 ± 0.065
3.517PheGly: 3.517 ± 0.067
0.828PheHis: 0.828 ± 0.031
1.082PheIle: 1.082 ± 0.041
0.931PheLys: 0.931 ± 0.039
5.086PheLeu: 5.086 ± 0.09
0.519PheMet: 0.519 ± 0.028
0.794PheAsn: 0.794 ± 0.037
2.496PhePro: 2.496 ± 0.062
1.223PheGln: 1.223 ± 0.039
2.981PheArg: 2.981 ± 0.065
2.895PheSer: 2.895 ± 0.066
2.033PheThr: 2.033 ± 0.052
3.137PheVal: 3.137 ± 0.062
0.866PheTrp: 0.866 ± 0.034
1.027PheTyr: 1.027 ± 0.038
0.0PheXaa: 0.0 ± 0.0
Gly
7.512GlyAla: 7.512 ± 0.112
0.957GlyCys: 0.957 ± 0.043
3.377GlyAsp: 3.377 ± 0.068
6.383GlyGlu: 6.383 ± 0.103
4.077GlyPhe: 4.077 ± 0.081
6.866GlyGly: 6.866 ± 0.11
1.603GlyHis: 1.603 ± 0.047
3.238GlyIle: 3.238 ± 0.074
3.958GlyLys: 3.958 ± 0.087
9.396GlyLeu: 9.396 ± 0.138
1.721GlyMet: 1.721 ± 0.047
1.901GlyAsn: 1.901 ± 0.06
3.749GlyPro: 3.749 ± 0.079
3.444GlyGln: 3.444 ± 0.067
5.6GlyArg: 5.6 ± 0.091
4.661GlySer: 4.661 ± 0.087
4.216GlyThr: 4.216 ± 0.089
7.55GlyVal: 7.55 ± 0.123
1.604GlyTrp: 1.604 ± 0.048
1.973GlyTyr: 1.973 ± 0.053
0.0GlyXaa: 0.0 ± 0.0
His
1.837HisAla: 1.837 ± 0.051
0.226HisCys: 0.226 ± 0.019
0.762HisAsp: 0.762 ± 0.039
1.016HisGlu: 1.016 ± 0.037
0.775HisPhe: 0.775 ± 0.031
1.744HisGly: 1.744 ± 0.051
0.564HisHis: 0.564 ± 0.032
0.654HisIle: 0.654 ± 0.03
0.487HisLys: 0.487 ± 0.026
2.57HisLeu: 2.57 ± 0.067
0.351HisMet: 0.351 ± 0.023
0.444HisAsn: 0.444 ± 0.024
1.758HisPro: 1.758 ± 0.057
0.778HisGln: 0.778 ± 0.034
1.834HisArg: 1.834 ± 0.049
0.769HisSer: 0.769 ± 0.032
0.852HisThr: 0.852 ± 0.033
1.543HisVal: 1.543 ± 0.039
0.363HisTrp: 0.363 ± 0.021
0.463HisTyr: 0.463 ± 0.024
0.0HisXaa: 0.0 ± 0.0
Ile
3.659IleAla: 3.659 ± 0.083
0.281IleCys: 0.281 ± 0.022
1.123IleAsp: 1.123 ± 0.041
1.778IleGlu: 1.778 ± 0.054
1.343IlePhe: 1.343 ± 0.043
2.351IleGly: 2.351 ± 0.063
0.696IleHis: 0.696 ± 0.036
1.159IleIle: 1.159 ± 0.046
1.046IleLys: 1.046 ± 0.042
3.833IleLeu: 3.833 ± 0.075
0.517IleMet: 0.517 ± 0.026
0.881IleAsn: 0.881 ± 0.032
2.438IlePro: 2.438 ± 0.061
0.957IleGln: 0.957 ± 0.037
2.384IleArg: 2.384 ± 0.062
1.646IleSer: 1.646 ± 0.044
2.016IleThr: 2.016 ± 0.062
2.587IleVal: 2.587 ± 0.067
0.372IleTrp: 0.372 ± 0.022
0.76IleTyr: 0.76 ± 0.04
0.0IleXaa: 0.0 ± 0.0
Lys
4.438LysAla: 4.438 ± 0.08
0.168LysCys: 0.168 ± 0.014
1.451LysAsp: 1.451 ± 0.048
2.397LysGlu: 2.397 ± 0.063
0.81LysPhe: 0.81 ± 0.034
2.738LysGly: 2.738 ± 0.059
0.571LysHis: 0.571 ± 0.029
1.177LysIle: 1.177 ± 0.046
1.631LysLys: 1.631 ± 0.051
4.356LysLeu: 4.356 ± 0.082
0.457LysMet: 0.457 ± 0.024
0.805LysAsn: 0.805 ± 0.035
2.534LysPro: 2.534 ± 0.064
1.063LysGln: 1.063 ± 0.045
2.272LysArg: 2.272 ± 0.051
1.337LysSer: 1.337 ± 0.041
1.836LysThr: 1.836 ± 0.049
3.213LysVal: 3.213 ± 0.074
0.274LysTrp: 0.274 ± 0.019
0.516LysTyr: 0.516 ± 0.029
0.0LysXaa: 0.0 ± 0.0
Leu
18.279LeuAla: 18.279 ± 0.252
0.965LeuCys: 0.965 ± 0.037
4.708LeuAsp: 4.708 ± 0.073
8.164LeuGlu: 8.164 ± 0.118
4.25LeuPhe: 4.25 ± 0.077
10.071LeuGly: 10.071 ± 0.156
1.946LeuHis: 1.946 ± 0.052
3.027LeuIle: 3.027 ± 0.066
3.636LeuLys: 3.636 ± 0.075
14.262LeuLeu: 14.262 ± 0.211
1.571LeuMet: 1.571 ± 0.045
1.939LeuAsn: 1.939 ± 0.047
8.044LeuPro: 8.044 ± 0.113
3.971LeuGln: 3.971 ± 0.077
8.518LeuArg: 8.518 ± 0.124
7.238LeuSer: 7.238 ± 0.116
5.547LeuThr: 5.547 ± 0.076
10.589LeuVal: 10.589 ± 0.134
2.067LeuTrp: 2.067 ± 0.064
2.273LeuTyr: 2.273 ± 0.059
0.0LeuXaa: 0.0 ± 0.0
Met
2.301MetAla: 2.301 ± 0.062
0.098MetCys: 0.098 ± 0.011
0.727MetAsp: 0.727 ± 0.03
1.153MetGlu: 1.153 ± 0.04
0.411MetPhe: 0.411 ± 0.024
1.469MetGly: 1.469 ± 0.045
0.274MetHis: 0.274 ± 0.019
0.54MetIle: 0.54 ± 0.024
0.713MetLys: 0.713 ± 0.03
1.66MetLeu: 1.66 ± 0.045
0.272MetMet: 0.272 ± 0.022
0.394MetAsn: 0.394 ± 0.024
0.878MetPro: 0.878 ± 0.032
0.473MetGln: 0.473 ± 0.026
1.282MetArg: 1.282 ± 0.043
0.889MetSer: 0.889 ± 0.028
0.817MetThr: 0.817 ± 0.034
1.534MetVal: 1.534 ± 0.042
0.174MetTrp: 0.174 ± 0.015
0.253MetTyr: 0.253 ± 0.018
0.0MetXaa: 0.0 ± 0.0
Asn
2.095AsnAla: 2.095 ± 0.056
0.228AsnCys: 0.228 ± 0.017
0.83AsnAsp: 0.83 ± 0.039
0.945AsnGlu: 0.945 ± 0.036
0.865AsnPhe: 0.865 ± 0.036
1.685AsnGly: 1.685 ± 0.064
0.42AsnHis: 0.42 ± 0.024
0.76AsnIle: 0.76 ± 0.032
0.467AsnLys: 0.467 ± 0.027
2.782AsnLeu: 2.782 ± 0.066
0.287AsnMet: 0.287 ± 0.019
0.596AsnAsn: 0.596 ± 0.037
2.184AsnPro: 2.184 ± 0.058
0.734AsnGln: 0.734 ± 0.034
1.687AsnArg: 1.687 ± 0.049
0.872AsnSer: 0.872 ± 0.041
0.934AsnThr: 0.934 ± 0.033
1.761AsnVal: 1.761 ± 0.047
0.354AsnTrp: 0.354 ± 0.022
0.536AsnTyr: 0.536 ± 0.027
0.0AsnXaa: 0.0 ± 0.0
Pro
6.308ProAla: 6.308 ± 0.106
0.461ProCys: 0.461 ± 0.025
2.604ProAsp: 2.604 ± 0.055
5.613ProGlu: 5.613 ± 0.091
2.342ProPhe: 2.342 ± 0.053
5.36ProGly: 5.36 ± 0.093
1.328ProHis: 1.328 ± 0.049
1.705ProIle: 1.705 ± 0.044
1.93ProLys: 1.93 ± 0.059
6.221ProLeu: 6.221 ± 0.096
1.041ProMet: 1.041 ± 0.035
1.32ProAsn: 1.32 ± 0.043
4.173ProPro: 4.173 ± 0.101
2.848ProGln: 2.848 ± 0.064
3.83ProArg: 3.83 ± 0.082
3.06ProSer: 3.06 ± 0.07
2.863ProThr: 2.863 ± 0.074
5.379ProVal: 5.379 ± 0.085
1.274ProTrp: 1.274 ± 0.05
1.236ProTyr: 1.236 ± 0.042
0.0ProXaa: 0.0 ± 0.0
Gln
5.503GlnAla: 5.503 ± 0.108
0.224GlnCys: 0.224 ± 0.016
1.408GlnAsp: 1.408 ± 0.041
3.096GlnGlu: 3.096 ± 0.07
1.005GlnPhe: 1.005 ± 0.035
3.312GlnGly: 3.312 ± 0.074
0.718GlnHis: 0.718 ± 0.031
1.122GlnIle: 1.122 ± 0.038
1.699GlnLys: 1.699 ± 0.054
4.243GlnLeu: 4.243 ± 0.071
0.579GlnMet: 0.579 ± 0.028
0.724GlnAsn: 0.724 ± 0.033
2.065GlnPro: 2.065 ± 0.056
1.54GlnGln: 1.54 ± 0.052
3.071GlnArg: 3.071 ± 0.059
1.278GlnSer: 1.278 ± 0.046
1.533GlnThr: 1.533 ± 0.054
3.684GlnVal: 3.684 ± 0.079
0.613GlnTrp: 0.613 ± 0.03
0.652GlnTyr: 0.652 ± 0.036
0.0GlnXaa: 0.0 ± 0.0
Arg
6.951ArgAla: 6.951 ± 0.09
0.721ArgCys: 0.721 ± 0.037
2.855ArgAsp: 2.855 ± 0.062
6.198ArgGlu: 6.198 ± 0.11
3.765ArgPhe: 3.765 ± 0.065
5.32ArgGly: 5.32 ± 0.099
1.508ArgHis: 1.508 ± 0.046
2.634ArgIle: 2.634 ± 0.064
2.547ArgLys: 2.547 ± 0.053
9.416ArgLeu: 9.416 ± 0.12
1.42ArgMet: 1.42 ± 0.043
1.367ArgAsn: 1.367 ± 0.044
3.561ArgPro: 3.561 ± 0.072
3.39ArgGln: 3.39 ± 0.068
6.074ArgArg: 6.074 ± 0.102
3.11ArgSer: 3.11 ± 0.068
2.951ArgThr: 2.951 ± 0.06
6.388ArgVal: 6.388 ± 0.093
1.74ArgTrp: 1.74 ± 0.059
1.87ArgTyr: 1.87 ± 0.05
0.0ArgXaa: 0.0 ± 0.0
Ser
4.594SerAla: 4.594 ± 0.085
0.601SerCys: 0.601 ± 0.033
1.855SerAsp: 1.855 ± 0.054
2.845SerGlu: 2.845 ± 0.068
2.507SerPhe: 2.507 ± 0.056
5.32SerGly: 5.32 ± 0.1
1.023SerHis: 1.023 ± 0.037
1.284SerIle: 1.284 ± 0.039
1.369SerLys: 1.369 ± 0.044
6.475SerLeu: 6.475 ± 0.113
0.866SerMet: 0.866 ± 0.034
0.97SerAsn: 0.97 ± 0.043
3.584SerPro: 3.584 ± 0.078
2.181SerGln: 2.181 ± 0.049
3.95SerArg: 3.95 ± 0.068
2.818SerSer: 2.818 ± 0.065
2.115SerThr: 2.115 ± 0.056
3.621SerVal: 3.621 ± 0.073
1.202SerTrp: 1.202 ± 0.045
1.278SerTyr: 1.278 ± 0.047
0.0SerXaa: 0.0 ± 0.0
Thr
4.86ThrAla: 4.86 ± 0.096
0.572ThrCys: 0.572 ± 0.039
1.772ThrAsp: 1.772 ± 0.045
2.126ThrGlu: 2.126 ± 0.059
1.838ThrPhe: 1.838 ± 0.055
4.322ThrGly: 4.322 ± 0.072
1.018ThrHis: 1.018 ± 0.037
1.539ThrIle: 1.539 ± 0.045
1.205ThrLys: 1.205 ± 0.039
5.792ThrLeu: 5.792 ± 0.102
0.765ThrMet: 0.765 ± 0.028
1.114ThrAsn: 1.114 ± 0.043
3.376ThrPro: 3.376 ± 0.079
1.396ThrGln: 1.396 ± 0.047
2.933ThrArg: 2.933 ± 0.063
2.301ThrSer: 2.301 ± 0.06
2.561ThrThr: 2.561 ± 0.064
4.928ThrVal: 4.928 ± 0.089
0.771ThrTrp: 0.771 ± 0.035
1.22ThrTyr: 1.22 ± 0.044
0.0ThrXaa: 0.0 ± 0.0
Val
10.948ValAla: 10.948 ± 0.124
0.807ValCys: 0.807 ± 0.033
3.877ValAsp: 3.877 ± 0.075
6.331ValGlu: 6.331 ± 0.117
3.542ValPhe: 3.542 ± 0.079
6.895ValGly: 6.895 ± 0.106
1.594ValHis: 1.594 ± 0.047
2.897ValIle: 2.897 ± 0.058
2.75ValLys: 2.75 ± 0.068
10.528ValLeu: 10.528 ± 0.142
1.42ValMet: 1.42 ± 0.038
1.842ValAsn: 1.842 ± 0.048
5.025ValPro: 5.025 ± 0.081
2.703ValGln: 2.703 ± 0.066
6.411ValArg: 6.411 ± 0.115
4.713ValSer: 4.713 ± 0.092
4.429ValThr: 4.429 ± 0.087
9.909ValVal: 9.909 ± 0.146
1.297ValTrp: 1.297 ± 0.045
1.837ValTyr: 1.837 ± 0.054
0.0ValXaa: 0.0 ± 0.0
Trp
1.805TrpAla: 1.805 ± 0.056
0.168TrpCys: 0.168 ± 0.015
0.766TrpAsp: 0.766 ± 0.031
1.255TrpGlu: 1.255 ± 0.043
0.624TrpPhe: 0.624 ± 0.029
1.442TrpGly: 1.442 ± 0.051
0.343TrpHis: 0.343 ± 0.024
0.51TrpIle: 0.51 ± 0.026
0.464TrpLys: 0.464 ± 0.025
2.109TrpLeu: 2.109 ± 0.062
0.271TrpMet: 0.271 ± 0.018
0.391TrpAsn: 0.391 ± 0.026
0.971TrpPro: 0.971 ± 0.038
1.005TrpGln: 1.005 ± 0.038
1.274TrpArg: 1.274 ± 0.042
0.806TrpSer: 0.806 ± 0.035
0.603TrpThr: 0.603 ± 0.029
1.641TrpVal: 1.641 ± 0.051
0.388TrpTrp: 0.388 ± 0.022
0.321TrpTyr: 0.321 ± 0.022
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.989TyrAla: 1.989 ± 0.051
0.207TyrCys: 0.207 ± 0.017
1.107TyrAsp: 1.107 ± 0.046
1.101TyrGlu: 1.101 ± 0.039
0.986TyrPhe: 0.986 ± 0.038
1.903TyrGly: 1.903 ± 0.064
0.491TyrHis: 0.491 ± 0.025
0.604TyrIle: 0.604 ± 0.032
0.494TyrLys: 0.494 ± 0.024
2.755TyrLeu: 2.755 ± 0.069
0.306TyrMet: 0.306 ± 0.02
0.536TyrAsn: 0.536 ± 0.032
1.375TyrPro: 1.375 ± 0.045
0.976TyrGln: 0.976 ± 0.036
2.016TyrArg: 2.016 ± 0.043
0.989TyrSer: 0.989 ± 0.045
1.021TyrThr: 1.021 ± 0.038
1.575TyrVal: 1.575 ± 0.047
0.334TyrTrp: 0.334 ± 0.023
0.587TyrTyr: 0.587 ± 0.035
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2252 proteins (781730 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski