Candidatus Aramenus sulfurataquae
Average proteome isoelectric point is 6.85
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1975 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|W7KK15|W7KK15_9CREN NADH dehydrogenase OS=Candidatus Aramenus sulfurataquae OX=1326980 GN=ASUL_04259 PE=4 SV=1
MM1 pKa = 7.49 AGEE4 pKa = 4.48 VISEE8 pKa = 4.67 GILIIASIALVGILAGVIFYY28 pKa = 10.61 AITTIGSGLDD38 pKa = 3.47 SMSTAISQKK47 pKa = 10.42 LATDD51 pKa = 3.79 LEE53 pKa = 4.71 IIYY56 pKa = 8.01 ATNTSPTEE64 pKa = 3.87 VVFYY68 pKa = 10.23 LQNLGQTPIYY78 pKa = 10.06 LSSSQVYY85 pKa = 9.57 FGKK88 pKa = 10.51 LYY90 pKa = 9.93 SLQPIGYY97 pKa = 9.08 SGTPPSWSSQTTVLNPGQTAEE118 pKa = 4.14 ITISLSTKK126 pKa = 9.65 LQQNQDD132 pKa = 3.58 YY133 pKa = 10.07 EE134 pKa = 4.79 IMFVAPNGYY143 pKa = 6.79 EE144 pKa = 3.71 TTYY147 pKa = 10.75 VFEE150 pKa = 4.57 VVV152 pKa = 3.19
Molecular weight: 16.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.973
IPC2_protein 4.024
IPC_protein 3.834
Toseland 3.681
ProMoST 3.973
Dawson 3.795
Bjellqvist 3.961
Wikipedia 3.681
Rodwell 3.681
Grimsley 3.605
Solomon 3.757
Lehninger 3.706
Nozaki 3.935
DTASelect 3.999
Thurlkill 3.732
EMBOSS 3.706
Sillero 3.948
Patrickios 0.006
IPC_peptide 3.77
IPC2_peptide 3.923
IPC2.peptide.svr19 3.861
Protein with the highest isoelectric point:
>tr|W7KMJ0|W7KMJ0_9CREN Sec-independent translocation protein OS=Candidatus Aramenus sulfurataquae OX=1326980 GN=ASUL_03079 PE=4 SV=1
MM1 pKa = 6.71 VRR3 pKa = 11.84 RR4 pKa = 11.84 MGKK7 pKa = 9.53 YY8 pKa = 9.25 KK9 pKa = 10.24 PPAEE13 pKa = 3.98 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 5.21 GKK18 pKa = 9.69 GVQEE22 pKa = 4.35 CRR24 pKa = 11.84 RR25 pKa = 11.84 CGSRR29 pKa = 11.84 DD30 pKa = 3.51 SVIQKK35 pKa = 10.27 YY36 pKa = 10.39 GIYY39 pKa = 10.4 LCRR42 pKa = 11.84 QCFRR46 pKa = 11.84 EE47 pKa = 3.88 VAYY50 pKa = 10.03 PMGFKK55 pKa = 10.6 RR56 pKa = 11.84 LRR58 pKa = 3.54
Molecular weight: 6.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.35
IPC2_protein 9.809
IPC_protein 10.628
Toseland 10.613
ProMoST 10.613
Dawson 10.745
Bjellqvist 10.496
Wikipedia 10.965
Rodwell 10.95
Grimsley 10.804
Solomon 10.847
Lehninger 10.804
Nozaki 10.628
DTASelect 10.467
Thurlkill 10.628
EMBOSS 11.008
Sillero 10.672
Patrickios 10.687
IPC_peptide 10.847
IPC2_peptide 9.78
IPC2.peptide.svr19 8.445
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1975
0
1975
530875
25
1453
268.8
30.16
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.586 ± 0.051
0.63 ± 0.021
4.716 ± 0.041
7.601 ± 0.067
4.39 ± 0.043
7.07 ± 0.047
1.281 ± 0.022
6.884 ± 0.053
7.409 ± 0.071
10.24 ± 0.065
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.314 ± 0.027
3.85 ± 0.046
4.079 ± 0.041
2.114 ± 0.027
5.035 ± 0.048
6.657 ± 0.051
4.566 ± 0.055
9.302 ± 0.054
0.976 ± 0.02
4.3 ± 0.044
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here