Candidatus Nitrospira inopinata

Taxonomy: cellular organisms; Bacteria; Nitrospirae; Nitrospira; Nitrospirales; Nitrospiraceae; Nitrospira

Average proteome isoelectric point is 6.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3371 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0S4KRW4|A0A0S4KRW4_9BACT Uncharacterized protein OS=Candidatus Nitrospira inopinata OX=1715989 GN=NITINOP_2215 PE=4 SV=1
MM1 pKa = 7.68RR2 pKa = 11.84WFNFVGWGMLALAVMLDD19 pKa = 3.18EE20 pKa = 4.52WLYY23 pKa = 10.81LYY25 pKa = 10.62LWEE28 pKa = 4.42EE29 pKa = 4.29FNVGVVVAAAIYY41 pKa = 9.53LVVAIGLSVWGGRR54 pKa = 11.84STDD57 pKa = 3.44PDD59 pKa = 3.3

Molecular weight: 6.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0S4KX91|A0A0S4KX91_9BACT TIR domain-containing protein OS=Candidatus Nitrospira inopinata OX=1715989 GN=NITINOP_2996 PE=4 SV=1
MM1 pKa = 7.62KK2 pKa = 10.14MKK4 pKa = 8.76THH6 pKa = 6.83KK7 pKa = 10.46GASKK11 pKa = 10.55RR12 pKa = 11.84FAKK15 pKa = 9.86TGSGKK20 pKa = 9.67IVRR23 pKa = 11.84RR24 pKa = 11.84KK25 pKa = 9.24AGKK28 pKa = 9.56RR29 pKa = 11.84HH30 pKa = 5.68LLSHH34 pKa = 7.22KK35 pKa = 10.39KK36 pKa = 9.59RR37 pKa = 11.84DD38 pKa = 3.41RR39 pKa = 11.84KK40 pKa = 10.22RR41 pKa = 11.84RR42 pKa = 11.84LSGAAVVVGRR52 pKa = 11.84ATAVLNQLLSKK63 pKa = 10.89

Molecular weight: 7.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3371

0

3371

990833

20

3074

293.9

32.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.906 ± 0.046

1.03 ± 0.015

5.256 ± 0.032

6.272 ± 0.036

3.595 ± 0.023

8.081 ± 0.048

2.294 ± 0.025

5.137 ± 0.032

3.944 ± 0.033

10.447 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.348 ± 0.023

2.507 ± 0.023

5.253 ± 0.035

3.494 ± 0.023

7.634 ± 0.048

5.663 ± 0.032

5.398 ± 0.029

7.918 ± 0.039

1.354 ± 0.019

2.47 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski