Epulopiscium sp. AS2M-Bin001

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Epulopiscium; unclassified Epulopiscium

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2165 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S9CFF4|A0A1S9CFF4_9FIRM Uncharacterized protein OS=Epulopiscium sp. AS2M-Bin001 OX=1764958 GN=ATN31_10760 PE=4 SV=1
MM1 pKa = 7.76KK2 pKa = 10.15KK3 pKa = 9.46ICLLASLFLTMSTNILADD21 pKa = 3.13SHH23 pKa = 7.22LFEE26 pKa = 4.64RR27 pKa = 11.84TATAIVADD35 pKa = 4.31YY36 pKa = 11.67VNVDD40 pKa = 3.88PKK42 pKa = 11.11NITISEE48 pKa = 4.19INSTDD53 pKa = 3.57DD54 pKa = 3.23QSTLHH59 pKa = 7.56IEE61 pKa = 4.41FFSPVIFSGFSFYY74 pKa = 11.09QLFKK78 pKa = 10.4CTLDD82 pKa = 3.5AQTGEE87 pKa = 4.29ILSSNLGANGYY98 pKa = 8.49ATEE101 pKa = 4.65LEE103 pKa = 4.54VPITPIQIEE112 pKa = 4.39TSNKK116 pKa = 8.37SHH118 pKa = 6.73SYY120 pKa = 9.24WEE122 pKa = 4.16SLVDD126 pKa = 4.35LNEE129 pKa = 4.03ALEE132 pKa = 4.37PCLEE136 pKa = 4.02GLEE139 pKa = 3.98WDD141 pKa = 4.34DD142 pKa = 5.94FINSTLADD150 pKa = 4.63FYY152 pKa = 11.36EE153 pKa = 4.39LEE155 pKa = 4.12IDD157 pKa = 4.86FKK159 pKa = 11.22QLVYY163 pKa = 10.92SHH165 pKa = 7.03GIEE168 pKa = 4.14DD169 pKa = 5.7LIYY172 pKa = 10.89HH173 pKa = 7.12LDD175 pKa = 3.34IKK177 pKa = 10.6DD178 pKa = 3.02WHH180 pKa = 6.25EE181 pKa = 4.62FISILEE187 pKa = 4.05DD188 pKa = 4.1EE189 pKa = 4.7IDD191 pKa = 3.43IVLDD195 pKa = 3.48NATSSLYY202 pKa = 10.51SDD204 pKa = 4.41YY205 pKa = 11.31NITQPEE211 pKa = 4.52LIEE214 pKa = 4.1TFEE217 pKa = 4.44FEE219 pKa = 3.98LTAITNEE226 pKa = 3.93WLDD229 pKa = 3.3NWKK232 pKa = 10.6SEE234 pKa = 3.91WGPKK238 pKa = 10.08LGVEE242 pKa = 5.22FKK244 pKa = 10.4MSYY247 pKa = 11.39DD248 pKa = 3.32NTDD251 pKa = 3.33INWYY255 pKa = 9.87NISDD259 pKa = 3.96NLDD262 pKa = 3.1QGLEE266 pKa = 4.07EE267 pKa = 6.03FSEE270 pKa = 4.5EE271 pKa = 3.97WQAGWEE277 pKa = 4.07NTLDD281 pKa = 3.02IWQDD285 pKa = 2.76ISDD288 pKa = 3.58NWKK291 pKa = 10.66SFWDD295 pKa = 3.62SLDD298 pKa = 3.4FF299 pKa = 5.13

Molecular weight: 34.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S9CD82|A0A1S9CD82_9FIRM V-type ATP synthase beta chain OS=Epulopiscium sp. AS2M-Bin001 OX=1764958 GN=atpB PE=3 SV=1
MM1 pKa = 7.99AKK3 pKa = 10.23RR4 pKa = 11.84SMKK7 pKa = 10.21VKK9 pKa = 9.1QQRR12 pKa = 11.84PAKK15 pKa = 9.97FSTQAYY21 pKa = 8.53SRR23 pKa = 11.84CKK25 pKa = 9.47ICGRR29 pKa = 11.84PHH31 pKa = 7.33AYY33 pKa = 8.2IRR35 pKa = 11.84KK36 pKa = 8.92YY37 pKa = 10.18GICRR41 pKa = 11.84ICFRR45 pKa = 11.84EE46 pKa = 3.97LAYY49 pKa = 9.84KK50 pKa = 10.19GQIPGVKK57 pKa = 9.25KK58 pKa = 10.9ASWW61 pKa = 3.03

Molecular weight: 7.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2165

0

2165

826884

37

7665

381.9

42.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.365 ± 0.057

1.025 ± 0.022

6.036 ± 0.052

6.962 ± 0.058

4.4 ± 0.039

6.101 ± 0.059

1.646 ± 0.021

9.448 ± 0.067

7.286 ± 0.054

9.162 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.532 ± 0.03

6.222 ± 0.051

3.392 ± 0.037

3.162 ± 0.03

3.023 ± 0.035

6.447 ± 0.054

5.817 ± 0.066

6.032 ± 0.042

0.819 ± 0.017

4.122 ± 0.042
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski