Amino acid dipepetide frequency for Epulopiscium sp. AS2M-Bin001

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.941AlaAla: 3.941 ± 0.096
0.608AlaCys: 0.608 ± 0.03
3.418AlaAsp: 3.418 ± 0.106
4.123AlaGlu: 4.123 ± 0.096
2.865AlaPhe: 2.865 ± 0.094
3.955AlaGly: 3.955 ± 0.081
1.053AlaHis: 1.053 ± 0.033
6.257AlaIle: 6.257 ± 0.102
5.142AlaLys: 5.142 ± 0.095
5.962AlaLeu: 5.962 ± 0.102
1.768AlaMet: 1.768 ± 0.057
3.725AlaAsn: 3.725 ± 0.085
1.862AlaPro: 1.862 ± 0.051
2.501AlaGln: 2.501 ± 0.08
1.917AlaArg: 1.917 ± 0.051
3.756AlaSer: 3.756 ± 0.078
4.198AlaThr: 4.198 ± 0.089
3.741AlaVal: 3.741 ± 0.072
0.469AlaTrp: 0.469 ± 0.028
2.387AlaTyr: 2.387 ± 0.064
0.0AlaXaa: 0.0 ± 0.0
Cys
0.623CysAla: 0.623 ± 0.03
0.149CysCys: 0.149 ± 0.019
0.642CysAsp: 0.642 ± 0.033
0.674CysGlu: 0.674 ± 0.033
0.468CysPhe: 0.468 ± 0.024
0.832CysGly: 0.832 ± 0.041
0.202CysHis: 0.202 ± 0.018
0.905CysIle: 0.905 ± 0.036
0.629CysLys: 0.629 ± 0.029
0.837CysLeu: 0.837 ± 0.033
0.248CysMet: 0.248 ± 0.017
0.545CysAsn: 0.545 ± 0.033
0.374CysPro: 0.374 ± 0.026
0.271CysGln: 0.271 ± 0.018
0.271CysArg: 0.271 ± 0.018
0.818CysSer: 0.818 ± 0.036
0.556CysThr: 0.556 ± 0.028
0.626CysVal: 0.626 ± 0.032
0.075CysTrp: 0.075 ± 0.009
0.504CysTyr: 0.504 ± 0.042
0.0CysXaa: 0.0 ± 0.0
Asp
3.259AspAla: 3.259 ± 0.073
0.547AspCys: 0.547 ± 0.028
3.259AspAsp: 3.259 ± 0.11
4.34AspGlu: 4.34 ± 0.092
2.983AspPhe: 2.983 ± 0.065
3.806AspGly: 3.806 ± 0.09
0.931AspHis: 0.931 ± 0.033
6.124AspIle: 6.124 ± 0.096
4.45AspLys: 4.45 ± 0.087
5.972AspLeu: 5.972 ± 0.116
1.547AspMet: 1.547 ± 0.044
3.609AspAsn: 3.609 ± 0.087
2.338AspPro: 2.338 ± 0.073
1.852AspGln: 1.852 ± 0.062
1.764AspArg: 1.764 ± 0.047
3.536AspSer: 3.536 ± 0.084
3.164AspThr: 3.164 ± 0.079
3.547AspVal: 3.547 ± 0.067
0.55AspTrp: 0.55 ± 0.028
2.785AspTyr: 2.785 ± 0.075
0.0AspXaa: 0.0 ± 0.0
Glu
4.217GluAla: 4.217 ± 0.102
0.578GluCys: 0.578 ± 0.032
4.048GluAsp: 4.048 ± 0.094
5.407GluGlu: 5.407 ± 0.101
3.057GluPhe: 3.057 ± 0.073
3.54GluGly: 3.54 ± 0.074
1.182GluHis: 1.182 ± 0.04
7.205GluIle: 7.205 ± 0.125
5.401GluLys: 5.401 ± 0.095
6.83GluLeu: 6.83 ± 0.117
1.871GluMet: 1.871 ± 0.048
4.829GluAsn: 4.829 ± 0.104
1.893GluPro: 1.893 ± 0.051
2.457GluGln: 2.457 ± 0.059
1.968GluArg: 1.968 ± 0.055
3.657GluSer: 3.657 ± 0.085
3.494GluThr: 3.494 ± 0.078
4.385GluVal: 4.385 ± 0.08
0.588GluTrp: 0.588 ± 0.031
3.017GluTyr: 3.017 ± 0.067
0.0GluXaa: 0.0 ± 0.0
Phe
2.769PheAla: 2.769 ± 0.066
0.516PheCys: 0.516 ± 0.025
3.011PheAsp: 3.011 ± 0.07
3.119PheGlu: 3.119 ± 0.065
1.943PhePhe: 1.943 ± 0.056
2.888PheGly: 2.888 ± 0.066
0.668PheHis: 0.668 ± 0.028
4.042PheIle: 4.042 ± 0.082
3.207PheLys: 3.207 ± 0.065
3.633PheLeu: 3.633 ± 0.087
1.209PheMet: 1.209 ± 0.042
2.798PheAsn: 2.798 ± 0.059
1.229PhePro: 1.229 ± 0.042
1.104PheGln: 1.104 ± 0.04
1.311PheArg: 1.311 ± 0.05
3.175PheSer: 3.175 ± 0.082
2.482PheThr: 2.482 ± 0.064
2.722PheVal: 2.722 ± 0.057
0.362PheTrp: 0.362 ± 0.022
1.81PheTyr: 1.81 ± 0.048
0.0PheXaa: 0.0 ± 0.0
Gly
3.891GlyAla: 3.891 ± 0.095
0.826GlyCys: 0.826 ± 0.039
3.32GlyAsp: 3.32 ± 0.076
3.861GlyGlu: 3.861 ± 0.079
2.82GlyPhe: 2.82 ± 0.07
4.001GlyGly: 4.001 ± 0.114
1.201GlyHis: 1.201 ± 0.038
5.954GlyIle: 5.954 ± 0.092
4.186GlyLys: 4.186 ± 0.082
5.509GlyLeu: 5.509 ± 0.087
1.607GlyMet: 1.607 ± 0.049
3.027GlyAsn: 3.027 ± 0.067
1.553GlyPro: 1.553 ± 0.051
2.162GlyGln: 2.162 ± 0.05
2.07GlyArg: 2.07 ± 0.067
3.764GlySer: 3.764 ± 0.08
3.633GlyThr: 3.633 ± 0.094
4.25GlyVal: 4.25 ± 0.082
0.551GlyTrp: 0.551 ± 0.03
2.82GlyTyr: 2.82 ± 0.074
0.0GlyXaa: 0.0 ± 0.0
His
0.888HisAla: 0.888 ± 0.033
0.201HisCys: 0.201 ± 0.016
0.902HisAsp: 0.902 ± 0.035
1.058HisGlu: 1.058 ± 0.041
0.801HisPhe: 0.801 ± 0.028
1.073HisGly: 1.073 ± 0.039
0.353HisHis: 0.353 ± 0.02
1.457HisIle: 1.457 ± 0.043
1.121HisLys: 1.121 ± 0.039
1.537HisLeu: 1.537 ± 0.046
0.427HisMet: 0.427 ± 0.022
1.004HisAsn: 1.004 ± 0.039
0.799HisPro: 0.799 ± 0.036
0.469HisGln: 0.469 ± 0.025
0.514HisArg: 0.514 ± 0.029
1.062HisSer: 1.062 ± 0.043
0.911HisThr: 0.911 ± 0.035
0.977HisVal: 0.977 ± 0.03
0.186HisTrp: 0.186 ± 0.016
0.723HisTyr: 0.723 ± 0.032
0.0HisXaa: 0.0 ± 0.0
Ile
6.278IleAla: 6.278 ± 0.101
1.062IleCys: 1.062 ± 0.037
5.979IleAsp: 5.979 ± 0.106
6.772IleGlu: 6.772 ± 0.117
4.083IlePhe: 4.083 ± 0.088
5.771IleGly: 5.771 ± 0.115
1.368IleHis: 1.368 ± 0.04
8.617IleIle: 8.617 ± 0.141
6.728IleLys: 6.728 ± 0.109
8.615IleLeu: 8.615 ± 0.138
2.155IleMet: 2.155 ± 0.06
5.954IleAsn: 5.954 ± 0.088
3.495IlePro: 3.495 ± 0.091
2.662IleGln: 2.662 ± 0.058
2.8IleArg: 2.8 ± 0.071
6.868IleSer: 6.868 ± 0.12
5.5IleThr: 5.5 ± 0.109
5.513IleVal: 5.513 ± 0.089
0.681IleTrp: 0.681 ± 0.032
3.581IleTyr: 3.581 ± 0.069
0.0IleXaa: 0.0 ± 0.0
Lys
4.392LysAla: 4.392 ± 0.099
0.625LysCys: 0.625 ± 0.027
4.55LysAsp: 4.55 ± 0.085
5.663LysGlu: 5.663 ± 0.104
3.077LysPhe: 3.077 ± 0.06
3.626LysGly: 3.626 ± 0.063
1.142LysHis: 1.142 ± 0.041
7.02LysIle: 7.02 ± 0.11
5.517LysLys: 5.517 ± 0.111
6.724LysLeu: 6.724 ± 0.106
2.005LysMet: 2.005 ± 0.046
5.186LysAsn: 5.186 ± 0.094
2.004LysPro: 2.004 ± 0.055
2.358LysGln: 2.358 ± 0.068
2.275LysArg: 2.275 ± 0.062
4.261LysSer: 4.261 ± 0.072
3.904LysThr: 3.904 ± 0.081
4.58LysVal: 4.58 ± 0.081
0.61LysTrp: 0.61 ± 0.028
3.343LysTyr: 3.343 ± 0.074
0.0LysXaa: 0.0 ± 0.0
Leu
6.579LeuAla: 6.579 ± 0.104
0.969LeuCys: 0.969 ± 0.041
6.138LeuAsp: 6.138 ± 0.111
7.002LeuGlu: 7.002 ± 0.121
3.738LeuPhe: 3.738 ± 0.089
5.746LeuGly: 5.746 ± 0.096
1.346LeuHis: 1.346 ± 0.046
7.757LeuIle: 7.757 ± 0.112
7.023LeuLys: 7.023 ± 0.105
7.891LeuLeu: 7.891 ± 0.107
2.174LeuMet: 2.174 ± 0.06
5.528LeuAsn: 5.528 ± 0.1
3.389LeuPro: 3.389 ± 0.08
2.451LeuGln: 2.451 ± 0.07
2.794LeuArg: 2.794 ± 0.061
6.489LeuSer: 6.489 ± 0.103
5.429LeuThr: 5.429 ± 0.089
5.204LeuVal: 5.204 ± 0.078
0.599LeuTrp: 0.599 ± 0.028
3.377LeuTyr: 3.377 ± 0.066
0.0LeuXaa: 0.0 ± 0.0
Met
1.856MetAla: 1.856 ± 0.049
0.258MetCys: 0.258 ± 0.018
1.654MetAsp: 1.654 ± 0.045
1.485MetGlu: 1.485 ± 0.045
1.016MetPhe: 1.016 ± 0.041
1.379MetGly: 1.379 ± 0.044
0.464MetHis: 0.464 ± 0.027
2.171MetIle: 2.171 ± 0.064
2.009MetLys: 2.009 ± 0.049
2.437MetLeu: 2.437 ± 0.061
0.64MetMet: 0.64 ± 0.031
1.512MetAsn: 1.512 ± 0.042
1.074MetPro: 1.074 ± 0.039
0.941MetGln: 0.941 ± 0.035
0.795MetArg: 0.795 ± 0.031
1.645MetSer: 1.645 ± 0.044
1.371MetThr: 1.371 ± 0.047
1.415MetVal: 1.415 ± 0.059
0.227MetTrp: 0.227 ± 0.017
0.971MetTyr: 0.971 ± 0.043
0.0MetXaa: 0.0 ± 0.0
Asn
3.447AsnAla: 3.447 ± 0.083
0.612AsnCys: 0.612 ± 0.036
3.06AsnAsp: 3.06 ± 0.068
3.9AsnGlu: 3.9 ± 0.084
2.837AsnPhe: 2.837 ± 0.068
3.788AsnGly: 3.788 ± 0.084
1.03AsnHis: 1.03 ± 0.038
6.43AsnIle: 6.43 ± 0.108
4.432AsnLys: 4.432 ± 0.085
6.192AsnLeu: 6.192 ± 0.101
1.617AsnMet: 1.617 ± 0.054
4.203AsnAsn: 4.203 ± 0.096
2.661AsnPro: 2.661 ± 0.071
2.079AsnGln: 2.079 ± 0.057
1.728AsnArg: 1.728 ± 0.049
3.981AsnSer: 3.981 ± 0.091
3.482AsnThr: 3.482 ± 0.075
3.591AsnVal: 3.591 ± 0.074
0.532AsnTrp: 0.532 ± 0.029
2.616AsnTyr: 2.616 ± 0.055
0.0AsnXaa: 0.0 ± 0.0
Pro
2.079ProAla: 2.079 ± 0.058
0.279ProCys: 0.279 ± 0.021
2.352ProAsp: 2.352 ± 0.059
2.783ProGlu: 2.783 ± 0.07
1.454ProPhe: 1.454 ± 0.044
1.963ProGly: 1.963 ± 0.055
0.648ProHis: 0.648 ± 0.03
3.193ProIle: 3.193 ± 0.07
2.28ProLys: 2.28 ± 0.053
2.733ProLeu: 2.733 ± 0.075
0.73ProMet: 0.73 ± 0.032
2.276ProAsn: 2.276 ± 0.065
0.93ProPro: 0.93 ± 0.048
0.959ProGln: 0.959 ± 0.039
0.847ProArg: 0.847 ± 0.033
2.276ProSer: 2.276 ± 0.055
2.208ProThr: 2.208 ± 0.064
2.151ProVal: 2.151 ± 0.058
0.296ProTrp: 0.296 ± 0.018
1.486ProTyr: 1.486 ± 0.05
0.0ProXaa: 0.0 ± 0.0
Gln
2.18GlnAla: 2.18 ± 0.065
0.284GlnCys: 0.284 ± 0.021
1.814GlnAsp: 1.814 ± 0.052
2.217GlnGlu: 2.217 ± 0.058
1.211GlnPhe: 1.211 ± 0.042
1.63GlnGly: 1.63 ± 0.049
0.451GlnHis: 0.451 ± 0.021
3.098GlnIle: 3.098 ± 0.067
2.44GlnLys: 2.44 ± 0.074
2.865GlnLeu: 2.865 ± 0.067
0.911GlnMet: 0.911 ± 0.034
2.165GlnAsn: 2.165 ± 0.052
0.938GlnPro: 0.938 ± 0.036
1.101GlnGln: 1.101 ± 0.046
0.998GlnArg: 0.998 ± 0.037
1.896GlnSer: 1.896 ± 0.049
1.957GlnThr: 1.957 ± 0.051
1.837GlnVal: 1.837 ± 0.052
0.25GlnTrp: 0.25 ± 0.019
1.373GlnTyr: 1.373 ± 0.044
0.0GlnXaa: 0.0 ± 0.0
Arg
1.832ArgAla: 1.832 ± 0.049
0.325ArgCys: 0.325 ± 0.022
1.761ArgAsp: 1.761 ± 0.051
2.268ArgGlu: 2.268 ± 0.065
1.358ArgPhe: 1.358 ± 0.04
1.704ArgGly: 1.704 ± 0.045
0.48ArgHis: 0.48 ± 0.026
2.784ArgIle: 2.784 ± 0.063
2.26ArgLys: 2.26 ± 0.055
2.759ArgLeu: 2.759 ± 0.068
0.87ArgMet: 0.87 ± 0.035
1.704ArgAsn: 1.704 ± 0.048
1.064ArgPro: 1.064 ± 0.039
1.024ArgGln: 1.024 ± 0.039
1.107ArgArg: 1.107 ± 0.058
1.714ArgSer: 1.714 ± 0.044
1.676ArgThr: 1.676 ± 0.047
1.924ArgVal: 1.924 ± 0.059
0.267ArgTrp: 0.267 ± 0.02
1.353ArgTyr: 1.353 ± 0.043
0.0ArgXaa: 0.0 ± 0.0
Ser
4.198SerAla: 4.198 ± 0.096
0.574SerCys: 0.574 ± 0.031
3.929SerAsp: 3.929 ± 0.093
4.134SerGlu: 4.134 ± 0.096
2.794SerPhe: 2.794 ± 0.061
4.434SerGly: 4.434 ± 0.093
1.132SerHis: 1.132 ± 0.037
6.332SerIle: 6.332 ± 0.115
4.529SerLys: 4.529 ± 0.092
5.868SerLeu: 5.868 ± 0.104
1.647SerMet: 1.647 ± 0.045
3.897SerAsn: 3.897 ± 0.084
1.953SerPro: 1.953 ± 0.053
2.093SerGln: 2.093 ± 0.062
1.929SerArg: 1.929 ± 0.05
4.395SerSer: 4.395 ± 0.103
3.888SerThr: 3.888 ± 0.089
3.764SerVal: 3.764 ± 0.072
0.506SerTrp: 0.506 ± 0.025
2.478SerTyr: 2.478 ± 0.057
0.0SerXaa: 0.0 ± 0.0
Thr
3.814ThrAla: 3.814 ± 0.11
0.502ThrCys: 0.502 ± 0.027
3.391ThrAsp: 3.391 ± 0.09
3.558ThrGlu: 3.558 ± 0.088
2.589ThrPhe: 2.589 ± 0.066
3.918ThrGly: 3.918 ± 0.084
0.966ThrHis: 0.966 ± 0.036
5.555ThrIle: 5.555 ± 0.105
3.976ThrLys: 3.976 ± 0.081
5.672ThrLeu: 5.672 ± 0.086
1.293ThrMet: 1.293 ± 0.035
3.548ThrAsn: 3.548 ± 0.079
2.327ThrPro: 2.327 ± 0.061
1.966ThrGln: 1.966 ± 0.051
1.666ThrArg: 1.666 ± 0.048
3.756ThrSer: 3.756 ± 0.093
3.672ThrThr: 3.672 ± 0.098
3.483ThrVal: 3.483 ± 0.083
0.491ThrTrp: 0.491 ± 0.03
2.028ThrTyr: 2.028 ± 0.055
0.0ThrXaa: 0.0 ± 0.0
Val
4.476ValAla: 4.476 ± 0.084
0.751ValCys: 0.751 ± 0.036
3.956ValAsp: 3.956 ± 0.071
4.103ValGlu: 4.103 ± 0.077
2.463ValPhe: 2.463 ± 0.068
3.884ValGly: 3.884 ± 0.078
0.955ValHis: 0.955 ± 0.035
5.273ValIle: 5.273 ± 0.084
4.062ValLys: 4.062 ± 0.08
5.156ValLeu: 5.156 ± 0.079
1.46ValMet: 1.46 ± 0.045
3.484ValAsn: 3.484 ± 0.068
2.262ValPro: 2.262 ± 0.053
1.547ValGln: 1.547 ± 0.049
1.924ValArg: 1.924 ± 0.051
4.183ValSer: 4.183 ± 0.082
3.812ValThr: 3.812 ± 0.095
3.83ValVal: 3.83 ± 0.082
0.422ValTrp: 0.422 ± 0.026
2.316ValTyr: 2.316 ± 0.059
0.0ValXaa: 0.0 ± 0.0
Trp
0.579TrpAla: 0.579 ± 0.03
0.11TrpCys: 0.11 ± 0.014
0.544TrpAsp: 0.544 ± 0.03
0.415TrpGlu: 0.415 ± 0.027
0.394TrpPhe: 0.394 ± 0.025
0.536TrpGly: 0.536 ± 0.034
0.179TrpHis: 0.179 ± 0.014
0.691TrpIle: 0.691 ± 0.029
0.446TrpLys: 0.446 ± 0.025
0.675TrpLeu: 0.675 ± 0.031
0.226TrpMet: 0.226 ± 0.016
0.594TrpAsn: 0.594 ± 0.032
0.249TrpPro: 0.249 ± 0.019
0.34TrpGln: 0.34 ± 0.02
0.238TrpArg: 0.238 ± 0.017
0.548TrpSer: 0.548 ± 0.027
0.496TrpThr: 0.496 ± 0.031
0.44TrpVal: 0.44 ± 0.027
0.105TrpTrp: 0.105 ± 0.01
0.383TrpTyr: 0.383 ± 0.027
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.35TyrAla: 2.35 ± 0.049
0.472TyrCys: 0.472 ± 0.025
2.636TyrAsp: 2.636 ± 0.076
2.742TyrGlu: 2.742 ± 0.076
2.047TyrPhe: 2.047 ± 0.059
2.535TyrGly: 2.535 ± 0.065
0.711TyrHis: 0.711 ± 0.031
3.617TyrIle: 3.617 ± 0.075
3.021TyrLys: 3.021 ± 0.078
3.755TyrLeu: 3.755 ± 0.084
0.913TyrMet: 0.913 ± 0.039
2.63TyrAsn: 2.63 ± 0.058
1.531TyrPro: 1.531 ± 0.055
1.278TyrGln: 1.278 ± 0.039
1.319TyrArg: 1.319 ± 0.044
2.693TyrSer: 2.693 ± 0.066
2.341TyrThr: 2.341 ± 0.062
2.339TyrVal: 2.339 ± 0.051
0.421TyrTrp: 0.421 ± 0.026
1.87TyrTyr: 1.87 ± 0.068
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2165 proteins (826884 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski