Deminuibacter soli
Average proteome isoelectric point is 6.95
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4790 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3E1NER1|A0A3E1NER1_9BACT Cytochrome oxidase subunit I OS=Deminuibacter soli OX=2291815 GN=DXN05_19780 PE=4 SV=1
MM1 pKa = 8.12 DD2 pKa = 3.9 NQKK5 pKa = 10.76 KK6 pKa = 10.36 GGLPGTPKK14 pKa = 10.7 DD15 pKa = 3.13 RR16 pKa = 11.84 LTFNKK21 pKa = 8.99 TDD23 pKa = 3.44 QYY25 pKa = 11.13 IDD27 pKa = 3.07 AAAPIDD33 pKa = 4.13 LSHH36 pKa = 6.76 GCVAILVDD44 pKa = 3.7 VAADD48 pKa = 3.73 VVTDD52 pKa = 4.02 AATDD56 pKa = 3.54 AVTDD60 pKa = 3.87 AVVDD64 pKa = 4.77 GVADD68 pKa = 3.85 SAADD72 pKa = 3.61 AAADD76 pKa = 3.86 TAADD80 pKa = 3.72 AAADD84 pKa = 3.98 TAADD88 pKa = 3.87 TAADD92 pKa = 3.62 AAADD96 pKa = 3.72 AGADD100 pKa = 3.39 SGAQGGISTACSKK113 pKa = 10.53 RR114 pKa = 11.84 LKK116 pKa = 9.59 TLAIMLGGAIMSTVTTAVANAIYY139 pKa = 10.38 NAIKK143 pKa = 10.54 SAGSTPTTSADD154 pKa = 3.08 YY155 pKa = 7.7 WTALYY160 pKa = 11.2 NSMITVYY167 pKa = 10.36 PCNANNASTCPGNQRR182 pKa = 11.84 DD183 pKa = 3.88 EE184 pKa = 4.34 QEE186 pKa = 4.42 AMVLSLQQQLAQTASYY202 pKa = 9.43 AQSAQTFEE210 pKa = 4.7 EE211 pKa = 4.5 SWSAASQTQLKK222 pKa = 10.39 DD223 pKa = 3.33 SLITVTDD230 pKa = 3.39 TGGIPSMCQYY240 pKa = 9.78 MEE242 pKa = 4.61 NYY244 pKa = 8.38 TNTGASGAALVIATINVFDD263 pKa = 3.74 TVAAYY268 pKa = 7.95 EE269 pKa = 4.27 YY270 pKa = 10.35 PDD272 pKa = 3.47 LTNN275 pKa = 3.73
Molecular weight: 28.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.723
IPC2_protein 3.706
IPC_protein 3.745
Toseland 3.503
ProMoST 3.935
Dawson 3.757
Bjellqvist 3.91
Wikipedia 3.732
Rodwell 3.567
Grimsley 3.401
Solomon 3.745
Lehninger 3.706
Nozaki 3.872
DTASelect 4.177
Thurlkill 3.567
EMBOSS 3.732
Sillero 3.872
Patrickios 0.629
IPC_peptide 3.732
IPC2_peptide 3.834
IPC2.peptide.svr19 3.765
Protein with the highest isoelectric point:
>tr|A0A3E1NJ30|A0A3E1NJ30_9BACT Uncharacterized protein OS=Deminuibacter soli OX=2291815 GN=DXN05_10350 PE=4 SV=1
MM1 pKa = 7.17 TKK3 pKa = 9.84 WLFTEE8 pKa = 4.15 IDD10 pKa = 3.25 TAIILRR16 pKa = 11.84 VHH18 pKa = 6.61 QIQSGMPGQQPINSVLVYY36 pKa = 9.46 TCLLSGVQWFSVNTFEE52 pKa = 5.39 TRR54 pKa = 11.84 DD55 pKa = 3.29 RR56 pKa = 11.84 MEE58 pKa = 4.6 GIRR61 pKa = 11.84 RR62 pKa = 11.84 EE63 pKa = 3.98 RR64 pKa = 11.84 LKK66 pKa = 10.81 LVCFVRR72 pKa = 11.84 RR73 pKa = 11.84 NKK75 pKa = 9.93 VAIVLRR81 pKa = 11.84 KK82 pKa = 6.09 MTSEE86 pKa = 3.48 IRR88 pKa = 11.84 ILFF91 pKa = 3.99
Molecular weight: 10.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.344
IPC2_protein 9.472
IPC_protein 10.16
Toseland 10.716
ProMoST 10.379
Dawson 10.774
Bjellqvist 10.482
Wikipedia 10.979
Rodwell 10.965
Grimsley 10.804
Solomon 10.935
Lehninger 10.906
Nozaki 10.716
DTASelect 10.467
Thurlkill 10.701
EMBOSS 11.111
Sillero 10.716
Patrickios 10.76
IPC_peptide 10.935
IPC2_peptide 9.663
IPC2.peptide.svr19 8.637
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4790
0
4790
1704224
26
7259
355.8
39.66
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.818 ± 0.042
0.911 ± 0.013
5.075 ± 0.022
4.946 ± 0.038
4.589 ± 0.029
7.026 ± 0.039
2.189 ± 0.02
6.269 ± 0.027
5.909 ± 0.035
9.53 ± 0.045
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.316 ± 0.017
5.287 ± 0.037
4.128 ± 0.028
4.547 ± 0.026
4.222 ± 0.025
5.901 ± 0.032
6.194 ± 0.049
6.652 ± 0.035
1.316 ± 0.014
4.172 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here