Penicillium steckii
Average proteome isoelectric point is 6.26
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 10355 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1V6TZC2|A0A1V6TZC2_9EURO Uncharacterized protein OS=Penicillium steckii OX=303698 GN=PENSTE_c001G06324 PE=3 SV=1
MM1 pKa = 7.11 TLKK4 pKa = 10.77 LQALLTAASALLINGVAIAQPTRR27 pKa = 11.84 VIDD30 pKa = 3.45 TDD32 pKa = 3.79 FADD35 pKa = 3.84 PCVIQTDD42 pKa = 3.45 DD43 pKa = 3.81 GYY45 pKa = 11.77 YY46 pKa = 11.06 AFATSGNGVNVQIASSSDD64 pKa = 3.32 FASWEE69 pKa = 4.05 LLSGNDD75 pKa = 3.6 AMPGPFPSWVADD87 pKa = 4.29 TPAIWAPDD95 pKa = 3.62 VIQRR99 pKa = 11.84 DD100 pKa = 3.64 DD101 pKa = 3.78 GTFVMYY107 pKa = 8.94 FSAASADD114 pKa = 4.23 DD115 pKa = 4.16 DD116 pKa = 4.69 SKK118 pKa = 11.39 HH119 pKa = 6.26 CVGAATSSSITGPYY133 pKa = 8.7 TPEE136 pKa = 4.51 DD137 pKa = 3.59 NALACPLDD145 pKa = 3.6 KK146 pKa = 11.22 GGAIDD151 pKa = 5.27 ADD153 pKa = 4.01 GFKK156 pKa = 11.09 DD157 pKa = 3.11 GDD159 pKa = 3.8 TYY161 pKa = 11.49 YY162 pKa = 11.16 VVYY165 pKa = 10.45 KK166 pKa = 10.31 IDD168 pKa = 3.86 GNSIQSDD175 pKa = 4.11 STPLMLQGLNSDD187 pKa = 3.39 ATTANGDD194 pKa = 3.45 PTQLLDD200 pKa = 5.26 RR201 pKa = 11.84 DD202 pKa = 4.25 DD203 pKa = 3.89 TDD205 pKa = 4.0 GPLIEE210 pKa = 5.28 APSLANVDD218 pKa = 3.23 GTYY221 pKa = 10.95 YY222 pKa = 11.08 LSFSSNVYY230 pKa = 7.94 STKK233 pKa = 10.75 DD234 pKa = 3.31 YY235 pKa = 11.21 DD236 pKa = 3.75 VSYY239 pKa = 9.31 ATASSLTGPYY249 pKa = 9.17 TKK251 pKa = 10.63 ARR253 pKa = 11.84 DD254 pKa = 3.5 PDD256 pKa = 3.71 APLLVSGDD264 pKa = 3.77 PSNVGDD270 pKa = 4.0 LGGPGGSDD278 pKa = 3.5 FNADD282 pKa = 2.74 GSKK285 pKa = 10.08 IVFHH289 pKa = 6.81 AFEE292 pKa = 5.02 NGEE295 pKa = 4.05 NMDD298 pKa = 3.9 KK299 pKa = 10.9 GRR301 pKa = 11.84 AMWVADD307 pKa = 3.51 ITCASGVITINN318 pKa = 3.16
Molecular weight: 33.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.684
IPC2_protein 3.63
IPC_protein 3.681
Toseland 3.427
ProMoST 3.884
Dawson 3.706
Bjellqvist 3.859
Wikipedia 3.694
Rodwell 3.503
Grimsley 3.338
Solomon 3.694
Lehninger 3.656
Nozaki 3.821
DTASelect 4.164
Thurlkill 3.503
EMBOSS 3.694
Sillero 3.808
Patrickios 0.922
IPC_peptide 3.681
IPC2_peptide 3.783
IPC2.peptide.svr19 3.739
Protein with the highest isoelectric point:
>tr|A0A1V6TP39|A0A1V6TP39_9EURO Copper homeostasis protein cutC homolog OS=Penicillium steckii OX=303698 GN=PENSTE_c004G02620 PE=3 SV=1
MM1 pKa = 7.6 LCFRR5 pKa = 11.84 CRR7 pKa = 11.84 AVPSLRR13 pKa = 11.84 ASVSTPRR20 pKa = 11.84 ALMYY24 pKa = 10.24 SASPFAVQSKK34 pKa = 10.38 AITSAFPSARR44 pKa = 11.84 PFSSTLLSTPVRR56 pKa = 11.84 PQQTQMLSRR65 pKa = 11.84 LPSTTLSATPSASSLLSQQSQQSRR89 pKa = 11.84 AFSATAHH96 pKa = 6.38 LGVKK100 pKa = 10.16 RR101 pKa = 11.84 NTFNPSRR108 pKa = 11.84 RR109 pKa = 11.84 VQKK112 pKa = 10.39 RR113 pKa = 11.84 RR114 pKa = 11.84 SGFLARR120 pKa = 11.84 NRR122 pKa = 11.84 SQKK125 pKa = 10.12 GRR127 pKa = 11.84 LVLIRR132 pKa = 11.84 RR133 pKa = 11.84 RR134 pKa = 11.84 LKK136 pKa = 10.43 GRR138 pKa = 11.84 KK139 pKa = 8.86 AMSWW143 pKa = 3.05
Molecular weight: 15.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.4
IPC2_protein 10.965
IPC_protein 12.442
Toseland 12.603
ProMoST 13.1
Dawson 12.603
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.237
Grimsley 12.647
Solomon 13.1
Lehninger 12.998
Nozaki 12.603
DTASelect 12.603
Thurlkill 12.603
EMBOSS 13.1
Sillero 12.603
Patrickios 11.959
IPC_peptide 13.1
IPC2_peptide 12.091
IPC2.peptide.svr19 9.107
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
10355
0
10355
5147105
8
6030
497.1
55.11
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.066 ± 0.019
1.241 ± 0.008
5.712 ± 0.017
6.165 ± 0.027
3.887 ± 0.015
6.786 ± 0.026
2.383 ± 0.01
5.261 ± 0.016
4.753 ± 0.022
8.9 ± 0.028
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.247 ± 0.008
3.946 ± 0.012
5.921 ± 0.027
4.084 ± 0.017
5.831 ± 0.02
8.666 ± 0.027
5.911 ± 0.021
5.927 ± 0.015
1.487 ± 0.009
2.825 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here