Amino acid dipepetide frequency for Penicillium steckii

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.884AlaAla: 7.884 ± 0.059
1.03AlaCys: 1.03 ± 0.015
3.971AlaAsp: 3.971 ± 0.031
4.861AlaGlu: 4.861 ± 0.045
3.087AlaPhe: 3.087 ± 0.027
5.506AlaGly: 5.506 ± 0.041
1.65AlaHis: 1.65 ± 0.018
4.359AlaIle: 4.359 ± 0.033
3.748AlaLys: 3.748 ± 0.033
7.408AlaLeu: 7.408 ± 0.049
1.981AlaMet: 1.981 ± 0.02
2.948AlaAsn: 2.948 ± 0.023
4.423AlaPro: 4.423 ± 0.046
3.214AlaGln: 3.214 ± 0.028
4.46AlaArg: 4.46 ± 0.033
7.066AlaSer: 7.066 ± 0.04
4.9AlaThr: 4.9 ± 0.029
4.959AlaVal: 4.959 ± 0.039
1.119AlaTrp: 1.119 ± 0.018
2.09AlaTyr: 2.09 ± 0.022
0.0AlaXaa: 0.0 ± 0.0
Cys
0.935CysAla: 0.935 ± 0.014
0.241CysCys: 0.241 ± 0.008
0.701CysAsp: 0.701 ± 0.014
0.61CysGlu: 0.61 ± 0.011
0.591CysPhe: 0.591 ± 0.013
0.91CysGly: 0.91 ± 0.015
0.331CysHis: 0.331 ± 0.008
0.749CysIle: 0.749 ± 0.013
0.505CysLys: 0.505 ± 0.01
1.317CysLeu: 1.317 ± 0.019
0.28CysMet: 0.28 ± 0.008
0.429CysAsn: 0.429 ± 0.009
0.631CysPro: 0.631 ± 0.011
0.485CysGln: 0.485 ± 0.011
0.717CysArg: 0.717 ± 0.013
0.958CysSer: 0.958 ± 0.015
0.678CysThr: 0.678 ± 0.013
0.788CysVal: 0.788 ± 0.014
0.198CysTrp: 0.198 ± 0.007
0.358CysTyr: 0.358 ± 0.008
0.0CysXaa: 0.0 ± 0.0
Asp
4.259AspAla: 4.259 ± 0.03
0.624AspCys: 0.624 ± 0.013
4.053AspAsp: 4.053 ± 0.043
4.396AspGlu: 4.396 ± 0.036
2.258AspPhe: 2.258 ± 0.021
3.906AspGly: 3.906 ± 0.038
1.318AspHis: 1.318 ± 0.019
3.337AspIle: 3.337 ± 0.028
2.321AspLys: 2.321 ± 0.022
5.24AspLeu: 5.24 ± 0.031
1.279AspMet: 1.279 ± 0.016
2.001AspAsn: 2.001 ± 0.019
3.321AspPro: 3.321 ± 0.029
2.132AspGln: 2.132 ± 0.022
3.068AspArg: 3.068 ± 0.032
4.531AspSer: 4.531 ± 0.035
3.035AspThr: 3.035 ± 0.025
3.509AspVal: 3.509 ± 0.029
0.891AspTrp: 0.891 ± 0.014
1.643AspTyr: 1.643 ± 0.018
0.0AspXaa: 0.0 ± 0.0
Glu
4.923GluAla: 4.923 ± 0.04
0.634GluCys: 0.634 ± 0.012
4.164GluAsp: 4.164 ± 0.041
5.291GluGlu: 5.291 ± 0.064
2.031GluPhe: 2.031 ± 0.022
3.563GluGly: 3.563 ± 0.03
1.336GluHis: 1.336 ± 0.014
3.348GluIle: 3.348 ± 0.026
3.823GluLys: 3.823 ± 0.038
5.018GluLeu: 5.018 ± 0.037
1.548GluMet: 1.548 ± 0.016
2.69GluAsn: 2.69 ± 0.025
2.765GluPro: 2.765 ± 0.051
2.45GluGln: 2.45 ± 0.026
3.62GluArg: 3.62 ± 0.036
4.737GluSer: 4.737 ± 0.038
3.725GluThr: 3.725 ± 0.034
3.35GluVal: 3.35 ± 0.029
0.901GluTrp: 0.901 ± 0.014
1.732GluTyr: 1.732 ± 0.022
0.0GluXaa: 0.0 ± 0.0
Phe
3.073PheAla: 3.073 ± 0.024
0.607PheCys: 0.607 ± 0.011
2.386PheAsp: 2.386 ± 0.022
2.218PheGlu: 2.218 ± 0.021
1.768PhePhe: 1.768 ± 0.022
2.972PheGly: 2.972 ± 0.026
0.971PheHis: 0.971 ± 0.014
2.014PheIle: 2.014 ± 0.025
1.52PheLys: 1.52 ± 0.018
3.693PheLeu: 3.693 ± 0.034
0.871PheMet: 0.871 ± 0.014
1.556PheAsn: 1.556 ± 0.018
2.069PhePro: 2.069 ± 0.022
1.511PheGln: 1.511 ± 0.017
1.932PheArg: 1.932 ± 0.018
3.189PheSer: 3.189 ± 0.025
2.215PheThr: 2.215 ± 0.02
2.438PheVal: 2.438 ± 0.024
0.672PheTrp: 0.672 ± 0.012
1.194PheTyr: 1.194 ± 0.017
0.0PheXaa: 0.0 ± 0.0
Gly
5.05GlyAla: 5.05 ± 0.047
0.886GlyCys: 0.886 ± 0.014
3.619GlyAsp: 3.619 ± 0.035
3.493GlyGlu: 3.493 ± 0.023
2.925GlyPhe: 2.925 ± 0.029
5.451GlyGly: 5.451 ± 0.06
1.678GlyHis: 1.678 ± 0.02
3.808GlyIle: 3.808 ± 0.03
3.33GlyLys: 3.33 ± 0.031
6.154GlyLeu: 6.154 ± 0.038
1.601GlyMet: 1.601 ± 0.016
2.664GlyAsn: 2.664 ± 0.026
3.332GlyPro: 3.332 ± 0.029
2.594GlyGln: 2.594 ± 0.026
3.82GlyArg: 3.82 ± 0.031
5.989GlySer: 5.989 ± 0.051
3.835GlyThr: 3.835 ± 0.033
4.257GlyVal: 4.257 ± 0.033
1.172GlyTrp: 1.172 ± 0.018
2.2GlyTyr: 2.2 ± 0.023
0.0GlyXaa: 0.0 ± 0.0
His
1.773HisAla: 1.773 ± 0.018
0.334HisCys: 0.334 ± 0.008
1.356HisAsp: 1.356 ± 0.017
1.372HisGlu: 1.372 ± 0.016
0.944HisPhe: 0.944 ± 0.014
1.688HisGly: 1.688 ± 0.018
0.825HisHis: 0.825 ± 0.016
1.249HisIle: 1.249 ± 0.015
0.87HisLys: 0.87 ± 0.013
2.267HisLeu: 2.267 ± 0.022
0.489HisMet: 0.489 ± 0.01
0.882HisAsn: 0.882 ± 0.016
1.657HisPro: 1.657 ± 0.021
1.007HisGln: 1.007 ± 0.014
1.507HisArg: 1.507 ± 0.018
1.944HisSer: 1.944 ± 0.02
1.252HisThr: 1.252 ± 0.018
1.339HisVal: 1.339 ± 0.016
0.365HisTrp: 0.365 ± 0.009
0.708HisTyr: 0.708 ± 0.013
0.0HisXaa: 0.0 ± 0.0
Ile
4.309IleAla: 4.309 ± 0.031
0.839IleCys: 0.839 ± 0.013
3.059IleAsp: 3.059 ± 0.026
3.086IleGlu: 3.086 ± 0.028
2.326IlePhe: 2.326 ± 0.026
3.464IleGly: 3.464 ± 0.033
1.332IleHis: 1.332 ± 0.017
2.802IleIle: 2.802 ± 0.031
2.203IleLys: 2.203 ± 0.021
4.936IleLeu: 4.936 ± 0.038
1.112IleMet: 1.112 ± 0.015
1.968IleAsn: 1.968 ± 0.017
3.346IlePro: 3.346 ± 0.025
2.099IleGln: 2.099 ± 0.022
2.814IleArg: 2.814 ± 0.026
4.374IleSer: 4.374 ± 0.034
2.893IleThr: 2.893 ± 0.028
3.259IleVal: 3.259 ± 0.026
0.812IleTrp: 0.812 ± 0.014
1.575IleTyr: 1.575 ± 0.018
0.0IleXaa: 0.0 ± 0.0
Lys
3.846LysAla: 3.846 ± 0.03
0.542LysCys: 0.542 ± 0.012
2.836LysAsp: 2.836 ± 0.025
3.368LysGlu: 3.368 ± 0.036
1.527LysPhe: 1.527 ± 0.018
2.879LysGly: 2.879 ± 0.027
1.073LysHis: 1.073 ± 0.014
2.415LysIle: 2.415 ± 0.023
3.174LysLys: 3.174 ± 0.049
3.892LysLeu: 3.892 ± 0.03
1.017LysMet: 1.017 ± 0.015
1.945LysAsn: 1.945 ± 0.019
2.618LysPro: 2.618 ± 0.026
1.854LysGln: 1.854 ± 0.022
3.182LysArg: 3.182 ± 0.028
3.771LysSer: 3.771 ± 0.032
2.829LysThr: 2.829 ± 0.025
2.695LysVal: 2.695 ± 0.026
0.678LysTrp: 0.678 ± 0.012
1.387LysTyr: 1.387 ± 0.018
0.0LysXaa: 0.0 ± 0.0
Leu
7.531LeuAla: 7.531 ± 0.044
1.191LeuCys: 1.191 ± 0.016
5.21LeuAsp: 5.21 ± 0.037
5.495LeuGlu: 5.495 ± 0.04
3.51LeuPhe: 3.51 ± 0.03
6.02LeuGly: 6.02 ± 0.038
2.251LeuHis: 2.251 ± 0.026
4.291LeuIle: 4.291 ± 0.033
4.123LeuLys: 4.123 ± 0.033
8.258LeuLeu: 8.258 ± 0.057
1.896LeuMet: 1.896 ± 0.019
3.432LeuAsn: 3.432 ± 0.027
5.301LeuPro: 5.301 ± 0.039
3.868LeuGln: 3.868 ± 0.032
5.467LeuArg: 5.467 ± 0.041
7.536LeuSer: 7.536 ± 0.041
4.703LeuThr: 4.703 ± 0.034
5.308LeuVal: 5.308 ± 0.04
1.219LeuTrp: 1.219 ± 0.017
2.394LeuTyr: 2.394 ± 0.023
0.0LeuXaa: 0.0 ± 0.0
Met
2.181MetAla: 2.181 ± 0.021
0.261MetCys: 0.261 ± 0.007
1.294MetAsp: 1.294 ± 0.017
1.336MetGlu: 1.336 ± 0.017
0.78MetPhe: 0.78 ± 0.012
1.543MetGly: 1.543 ± 0.018
0.482MetHis: 0.482 ± 0.009
1.149MetIle: 1.149 ± 0.018
1.083MetLys: 1.083 ± 0.015
1.848MetLeu: 1.848 ± 0.018
0.629MetMet: 0.629 ± 0.011
0.944MetAsn: 0.944 ± 0.015
1.274MetPro: 1.274 ± 0.018
0.901MetGln: 0.901 ± 0.014
1.261MetArg: 1.261 ± 0.016
1.995MetSer: 1.995 ± 0.019
1.387MetThr: 1.387 ± 0.019
1.318MetVal: 1.318 ± 0.018
0.269MetTrp: 0.269 ± 0.008
0.54MetTyr: 0.54 ± 0.012
0.0MetXaa: 0.0 ± 0.0
Asn
3.174AsnAla: 3.174 ± 0.024
0.474AsnCys: 0.474 ± 0.01
2.186AsnAsp: 2.186 ± 0.021
2.288AsnGlu: 2.288 ± 0.022
1.516AsnPhe: 1.516 ± 0.017
3.097AsnGly: 3.097 ± 0.033
0.916AsnHis: 0.916 ± 0.014
2.269AsnIle: 2.269 ± 0.023
1.654AsnLys: 1.654 ± 0.02
3.444AsnLeu: 3.444 ± 0.029
0.954AsnMet: 0.954 ± 0.016
1.615AsnAsn: 1.615 ± 0.019
2.586AsnPro: 2.586 ± 0.025
1.528AsnGln: 1.528 ± 0.021
2.015AsnArg: 2.015 ± 0.021
3.118AsnSer: 3.118 ± 0.027
2.364AsnThr: 2.364 ± 0.022
2.471AsnVal: 2.471 ± 0.024
0.629AsnTrp: 0.629 ± 0.012
1.159AsnTyr: 1.159 ± 0.015
0.0AsnXaa: 0.0 ± 0.0
Pro
4.851ProAla: 4.851 ± 0.051
0.525ProCys: 0.525 ± 0.013
3.178ProAsp: 3.178 ± 0.028
3.952ProGlu: 3.952 ± 0.044
2.154ProPhe: 2.154 ± 0.023
3.867ProGly: 3.867 ± 0.036
1.28ProHis: 1.28 ± 0.018
2.728ProIle: 2.728 ± 0.026
2.553ProLys: 2.553 ± 0.025
4.601ProLeu: 4.601 ± 0.033
1.107ProMet: 1.107 ± 0.017
2.357ProAsn: 2.357 ± 0.025
4.636ProPro: 4.636 ± 0.077
2.468ProGln: 2.468 ± 0.033
3.315ProArg: 3.315 ± 0.033
6.074ProSer: 6.074 ± 0.052
3.847ProThr: 3.847 ± 0.033
3.457ProVal: 3.457 ± 0.029
0.762ProTrp: 0.762 ± 0.014
1.498ProTyr: 1.498 ± 0.017
0.0ProXaa: 0.0 ± 0.0
Gln
3.273GlnAla: 3.273 ± 0.025
0.452GlnCys: 0.452 ± 0.01
2.127GlnAsp: 2.127 ± 0.02
2.489GlnGlu: 2.489 ± 0.026
1.379GlnPhe: 1.379 ± 0.015
2.458GlnGly: 2.458 ± 0.023
1.016GlnHis: 1.016 ± 0.016
2.115GlnIle: 2.115 ± 0.019
2.083GlnLys: 2.083 ± 0.021
3.414GlnLeu: 3.414 ± 0.027
0.979GlnMet: 0.979 ± 0.015
1.775GlnAsn: 1.775 ± 0.02
2.533GlnPro: 2.533 ± 0.033
2.314GlnGln: 2.314 ± 0.044
2.529GlnArg: 2.529 ± 0.027
3.555GlnSer: 3.555 ± 0.029
2.399GlnThr: 2.399 ± 0.024
2.142GlnVal: 2.142 ± 0.023
0.6GlnTrp: 0.6 ± 0.011
1.206GlnTyr: 1.206 ± 0.018
0.0GlnXaa: 0.0 ± 0.0
Arg
4.246ArgAla: 4.246 ± 0.029
0.686ArgCys: 0.686 ± 0.013
3.287ArgAsp: 3.287 ± 0.035
3.606ArgGlu: 3.606 ± 0.033
2.17ArgPhe: 2.17 ± 0.023
3.575ArgGly: 3.575 ± 0.035
1.506ArgHis: 1.506 ± 0.018
2.904ArgIle: 2.904 ± 0.024
3.262ArgLys: 3.262 ± 0.031
5.256ArgLeu: 5.256 ± 0.038
1.267ArgMet: 1.267 ± 0.018
2.307ArgAsn: 2.307 ± 0.022
3.315ArgPro: 3.315 ± 0.028
2.493ArgGln: 2.493 ± 0.026
4.73ArgArg: 4.73 ± 0.052
4.83ArgSer: 4.83 ± 0.045
3.077ArgThr: 3.077 ± 0.023
3.251ArgVal: 3.251 ± 0.027
0.92ArgTrp: 0.92 ± 0.014
1.619ArgTyr: 1.619 ± 0.016
0.0ArgXaa: 0.0 ± 0.0
Ser
6.506SerAla: 6.506 ± 0.041
0.937SerCys: 0.937 ± 0.016
4.555SerAsp: 4.555 ± 0.037
4.471SerGlu: 4.471 ± 0.033
3.304SerPhe: 3.304 ± 0.03
5.801SerGly: 5.801 ± 0.051
2.077SerHis: 2.077 ± 0.02
4.441SerIle: 4.441 ± 0.03
3.987SerLys: 3.987 ± 0.034
7.621SerLeu: 7.621 ± 0.044
1.877SerMet: 1.877 ± 0.021
3.449SerAsn: 3.449 ± 0.029
5.579SerPro: 5.579 ± 0.055
3.643SerGln: 3.643 ± 0.034
5.109SerArg: 5.109 ± 0.044
9.371SerSer: 9.371 ± 0.077
5.8SerThr: 5.8 ± 0.048
4.668SerVal: 4.668 ± 0.035
1.226SerTrp: 1.226 ± 0.016
2.233SerTyr: 2.233 ± 0.023
0.0SerXaa: 0.0 ± 0.0
Thr
4.86ThrAla: 4.86 ± 0.032
0.727ThrCys: 0.727 ± 0.014
2.937ThrAsp: 2.937 ± 0.024
3.25ThrGlu: 3.25 ± 0.027
2.248ThrPhe: 2.248 ± 0.023
4.239ThrGly: 4.239 ± 0.037
1.278ThrHis: 1.278 ± 0.015
3.217ThrIle: 3.217 ± 0.03
2.582ThrLys: 2.582 ± 0.024
5.153ThrLeu: 5.153 ± 0.034
1.234ThrMet: 1.234 ± 0.017
2.242ThrAsn: 2.242 ± 0.02
4.229ThrPro: 4.229 ± 0.043
2.149ThrGln: 2.149 ± 0.023
3.07ThrArg: 3.07 ± 0.026
5.457ThrSer: 5.457 ± 0.043
4.18ThrThr: 4.18 ± 0.051
3.622ThrVal: 3.622 ± 0.031
0.865ThrTrp: 0.865 ± 0.014
1.575ThrTyr: 1.575 ± 0.017
0.0ThrXaa: 0.0 ± 0.0
Val
4.765ValAla: 4.765 ± 0.035
0.799ValCys: 0.799 ± 0.013
3.641ValAsp: 3.641 ± 0.027
3.655ValGlu: 3.655 ± 0.03
2.541ValPhe: 2.541 ± 0.025
3.858ValGly: 3.858 ± 0.03
1.383ValHis: 1.383 ± 0.016
3.074ValIle: 3.074 ± 0.029
2.736ValLys: 2.736 ± 0.028
5.376ValLeu: 5.376 ± 0.037
1.315ValMet: 1.315 ± 0.016
2.372ValAsn: 2.372 ± 0.024
3.47ValPro: 3.47 ± 0.028
2.382ValGln: 2.382 ± 0.02
3.155ValArg: 3.155 ± 0.028
4.826ValSer: 4.826 ± 0.034
3.426ValThr: 3.426 ± 0.026
3.954ValVal: 3.954 ± 0.037
0.824ValTrp: 0.824 ± 0.015
1.715ValTyr: 1.715 ± 0.02
0.0ValXaa: 0.0 ± 0.0
Trp
1.118TrpAla: 1.118 ± 0.016
0.197TrpCys: 0.197 ± 0.006
0.896TrpAsp: 0.896 ± 0.015
0.844TrpGlu: 0.844 ± 0.014
0.554TrpPhe: 0.554 ± 0.011
0.949TrpGly: 0.949 ± 0.016
0.353TrpHis: 0.353 ± 0.009
0.862TrpIle: 0.862 ± 0.015
0.854TrpLys: 0.854 ± 0.014
1.375TrpLeu: 1.375 ± 0.018
0.386TrpMet: 0.386 ± 0.01
0.688TrpAsn: 0.688 ± 0.012
0.6TrpPro: 0.6 ± 0.011
0.567TrpGln: 0.567 ± 0.01
0.939TrpArg: 0.939 ± 0.014
1.154TrpSer: 1.154 ± 0.018
0.91TrpThr: 0.91 ± 0.015
0.884TrpVal: 0.884 ± 0.015
0.278TrpTrp: 0.278 ± 0.008
0.462TrpTyr: 0.462 ± 0.01
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.106TyrAla: 2.106 ± 0.023
0.426TyrCys: 0.426 ± 0.009
1.67TyrAsp: 1.67 ± 0.02
1.568TyrGlu: 1.568 ± 0.017
1.253TyrPhe: 1.253 ± 0.018
2.112TyrGly: 2.112 ± 0.021
0.772TyrHis: 0.772 ± 0.012
1.48TyrIle: 1.48 ± 0.02
1.118TyrLys: 1.118 ± 0.018
2.732TyrLeu: 2.732 ± 0.025
0.648TyrMet: 0.648 ± 0.012
1.196TyrAsn: 1.196 ± 0.014
1.525TyrPro: 1.525 ± 0.019
1.178TyrGln: 1.178 ± 0.015
1.601TyrArg: 1.601 ± 0.018
2.179TyrSer: 2.179 ± 0.022
1.657TyrThr: 1.657 ± 0.017
1.6TyrVal: 1.6 ± 0.019
0.469TyrTrp: 0.469 ± 0.011
0.962TyrTyr: 0.962 ± 0.015
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 10355 proteins (5147105 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski