Alteromonas pelagimontana
Average proteome isoelectric point is 6.15
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3617 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6M4MC23|A0A6M4MC23_9ALTE DUF1624 domain-containing protein OS=Alteromonas pelagimontana OX=1858656 GN=CA267_008035 PE=4 SV=1
MM1 pKa = 7.73 RR2 pKa = 11.84 KK3 pKa = 8.14 ITLSSRR9 pKa = 11.84 QLSKK13 pKa = 10.66 IALASALAFCSSQSHH28 pKa = 5.84 AAVGNLIWEE37 pKa = 4.7 DD38 pKa = 3.35 NFNSFNTDD46 pKa = 1.49 IWNIDD51 pKa = 3.6 EE52 pKa = 5.48 GDD54 pKa = 3.44 GCAQGLCGWGNQEE67 pKa = 4.2 LQWYY71 pKa = 8.36 AQDD74 pKa = 3.28 NVYY77 pKa = 10.32 IQDD80 pKa = 3.5 IAGEE84 pKa = 4.22 PGNKK88 pKa = 9.8 GLVLEE93 pKa = 5.12 ARR95 pKa = 11.84 NQATGGKK102 pKa = 9.33 AFTSGKK108 pKa = 8.98 IQSSNKK114 pKa = 9.67 LAIKK118 pKa = 10.41 YY119 pKa = 10.3 GMIEE123 pKa = 4.22 MRR125 pKa = 11.84 TKK127 pKa = 10.11 TPNVDD132 pKa = 3.05 TGLWPALWMLGTSTSSWPAKK152 pKa = 10.55 GEE154 pKa = 3.72 IDD156 pKa = 3.56 IMEE159 pKa = 4.65 MGQSAAQRR167 pKa = 11.84 ADD169 pKa = 2.91 AGFPGAPLNNYY180 pKa = 8.87 VGSNLIFYY188 pKa = 10.75 ADD190 pKa = 3.98 AACSDD195 pKa = 4.32 GNPSCAASTAWQTDD209 pKa = 3.33 NAHH212 pKa = 6.6 LSSSSLANRR221 pKa = 11.84 FVTYY225 pKa = 10.45 RR226 pKa = 11.84 LYY228 pKa = 8.99 WTEE231 pKa = 3.69 SQIRR235 pKa = 11.84 FAIVDD240 pKa = 3.49 GGVEE244 pKa = 4.22 YY245 pKa = 10.82 EE246 pKa = 4.7 MYY248 pKa = 10.26 SQPFTISEE256 pKa = 4.2 EE257 pKa = 4.36 SNEE260 pKa = 4.09 FQQPFYY266 pKa = 11.42 LLMDD270 pKa = 4.7 LAVGGTFTDD279 pKa = 3.53 AQTNGQVTAPLPAKK293 pKa = 9.83 MVVDD297 pKa = 3.68 YY298 pKa = 11.41 VRR300 pKa = 11.84 IYY302 pKa = 10.69 EE303 pKa = 4.35 LDD305 pKa = 3.53 GQGEE309 pKa = 4.3 IFLGNTVPEE318 pKa = 4.17 EE319 pKa = 3.99 TGTFGVFTDD328 pKa = 3.91 NTPTNNKK335 pKa = 9.73 LEE337 pKa = 4.74 PGVDD341 pKa = 2.94 ADD343 pKa = 3.66 IYY345 pKa = 10.96 VWNQTSITDD354 pKa = 3.81 GTTPPYY360 pKa = 10.85 EE361 pKa = 4.71 GDD363 pKa = 4.67 NVLAWQYY370 pKa = 11.63 SPSQWFGGGFQSRR383 pKa = 11.84 QPRR386 pKa = 11.84 DD387 pKa = 3.25 LSNFADD393 pKa = 3.73 GDD395 pKa = 3.91 VHH397 pKa = 7.15 FKK399 pKa = 10.65 IKK401 pKa = 10.37 IPADD405 pKa = 3.23 VSFKK409 pKa = 11.22 VGIADD414 pKa = 4.3 TYY416 pKa = 10.92 TNEE419 pKa = 3.53 NWIEE423 pKa = 4.05 FPANTTKK430 pKa = 10.93 YY431 pKa = 9.91 GLVRR435 pKa = 11.84 NGDD438 pKa = 3.16 WAEE441 pKa = 3.82 AVIPVSDD448 pKa = 3.76 LAGPLVALQSISNPFNIASVDD469 pKa = 3.84 GQIPTYY475 pKa = 9.98 AFEE478 pKa = 4.17 MALDD482 pKa = 4.81 DD483 pKa = 4.72 IVWTGGGGPVVQDD496 pKa = 3.64 SDD498 pKa = 4.09 GDD500 pKa = 4.33 GINDD504 pKa = 4.87 NDD506 pKa = 4.31 DD507 pKa = 3.56 QCPTTAGDD515 pKa = 3.84 AANNGCPASSNAIDD529 pKa = 3.94 LTDD532 pKa = 3.49 VPGTFTAQHH541 pKa = 6.39 NDD543 pKa = 2.85 SPANEE548 pKa = 4.18 GAEE551 pKa = 4.02 NAFDD555 pKa = 6.02 DD556 pKa = 4.52 DD557 pKa = 4.19 TTTKK561 pKa = 10.84 YY562 pKa = 9.94 LTFHH566 pKa = 6.69 ASAWLQYY573 pKa = 8.61 QTADD577 pKa = 3.25 FAYY580 pKa = 8.35 PASGYY585 pKa = 10.41 RR586 pKa = 11.84 ITSANDD592 pKa = 3.06 APEE595 pKa = 4.88 RR596 pKa = 11.84 DD597 pKa = 3.51 PMSWNFQGSSDD608 pKa = 3.56 GVTWTTLDD616 pKa = 3.37 SRR618 pKa = 11.84 SNEE621 pKa = 3.93 DD622 pKa = 2.94 FSSRR626 pKa = 11.84 FQTRR630 pKa = 11.84 EE631 pKa = 3.45 FSFSNTTAWQFYY643 pKa = 9.35 RR644 pKa = 11.84 INMMNNSGGMTQVAEE659 pKa = 4.15 FSILGSDD666 pKa = 3.47 NPVPVNQNSDD676 pKa = 3.17 SDD678 pKa = 4.17 GDD680 pKa = 4.23 GVADD684 pKa = 4.9 SADD687 pKa = 3.66 NCPDD691 pKa = 3.54 TPAGTQVDD699 pKa = 4.42 GNGCAISDD707 pKa = 3.72 GATGVEE713 pKa = 4.24 QVSADD718 pKa = 3.61 SIVFFVNTADD728 pKa = 3.52 WADD731 pKa = 3.21 VHH733 pKa = 5.46 YY734 pKa = 9.45 TINGQNQQNLRR745 pKa = 11.84 MNIEE749 pKa = 3.9 AGRR752 pKa = 11.84 NEE754 pKa = 3.79 TRR756 pKa = 11.84 VNGLSAGDD764 pKa = 4.05 TITYY768 pKa = 7.87 WFTYY772 pKa = 8.25 LQPNGAVTDD781 pKa = 4.23 TSPQTYY787 pKa = 10.27 SVVTTSAGDD796 pKa = 3.59 ADD798 pKa = 4.11 GDD800 pKa = 4.31 GVNDD804 pKa = 5.76 GVDD807 pKa = 3.2 QCANTPAGASVDD819 pKa = 3.66 EE820 pKa = 4.93 SGCEE824 pKa = 3.92 TLVTTSVVVEE834 pKa = 4.07 AEE836 pKa = 4.61 NYY838 pKa = 10.49 SNWNDD843 pKa = 2.92 SDD845 pKa = 3.58 AGNNGGAYY853 pKa = 10.4 RR854 pKa = 11.84 NDD856 pKa = 3.75 DD857 pKa = 3.43 VDD859 pKa = 4.4 IEE861 pKa = 4.35 PTTDD865 pKa = 2.76 INSGYY870 pKa = 9.7 NVGWTASGEE879 pKa = 4.06 WLEE882 pKa = 4.16 YY883 pKa = 10.86 AVTLGAGTYY892 pKa = 8.72 QVSSRR897 pKa = 11.84 VASNTGAGGYY907 pKa = 7.62 TVALNGAEE915 pKa = 3.92 FATDD919 pKa = 3.28 NVEE922 pKa = 4.77 ATGGWQSFVTHH933 pKa = 6.54 SLGQVTITEE942 pKa = 4.85 GGSQTLRR949 pKa = 11.84 INFTGADD956 pKa = 3.62 VNFNWLKK963 pKa = 10.98 FEE965 pKa = 4.82 LLGNN969 pKa = 4.2
Molecular weight: 104.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.69
IPC2_protein 3.808
IPC_protein 3.846
Toseland 3.617
ProMoST 4.012
Dawson 3.846
Bjellqvist 3.986
Wikipedia 3.77
Rodwell 3.668
Grimsley 3.528
Solomon 3.834
Lehninger 3.795
Nozaki 3.948
DTASelect 4.202
Thurlkill 3.668
EMBOSS 3.783
Sillero 3.973
Patrickios 1.354
IPC_peptide 3.834
IPC2_peptide 3.948
IPC2.peptide.svr19 3.85
Protein with the highest isoelectric point:
>tr|A0A6M4MDS1|A0A6M4MDS1_9ALTE Uncharacterized protein OS=Alteromonas pelagimontana OX=1858656 GN=CA267_010895 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLKK11 pKa = 10.14 RR12 pKa = 11.84 KK13 pKa = 9.13 RR14 pKa = 11.84 SHH16 pKa = 6.17 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.37 NGRR28 pKa = 11.84 KK29 pKa = 9.16 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.28 GRR39 pKa = 11.84 ARR41 pKa = 11.84 LCAA44 pKa = 3.92
Molecular weight: 5.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.403
IPC2_protein 10.862
IPC_protein 12.442
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.34
Grimsley 12.647
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.076
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.067
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3617
0
3617
1214117
22
2045
335.7
37.26
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.19 ± 0.041
0.977 ± 0.015
5.676 ± 0.035
6.187 ± 0.036
4.188 ± 0.028
6.836 ± 0.044
2.249 ± 0.02
6.047 ± 0.028
4.98 ± 0.033
9.996 ± 0.05
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.517 ± 0.02
4.243 ± 0.032
4.092 ± 0.024
4.48 ± 0.034
4.868 ± 0.031
6.53 ± 0.03
5.485 ± 0.03
7.011 ± 0.03
1.315 ± 0.018
3.132 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here