Alcanivorax dieselolei (strain DSM 16502 / CGMCC 1.3690 / B-5)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; Alcanivoracaceae; Alcanivorax; Alcanivorax dieselolei

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4388 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K0CCK1|K0CCK1_ALCDB Erythronate-4-phosphate dehydrogenase OS=Alcanivorax dieselolei (strain DSM 16502 / CGMCC 1.3690 / B-5) OX=930169 GN=pdxB PE=3 SV=1
MM1 pKa = 7.36IKK3 pKa = 10.17KK4 pKa = 9.77AALAAAIASAPFAASATDD22 pKa = 3.79LVGGGATLPAVAYY35 pKa = 10.07VGEE38 pKa = 5.16DD39 pKa = 3.39FTLTDD44 pKa = 3.54PQARR48 pKa = 11.84LSTNAGLNPGSGFLDD63 pKa = 3.71APLGIDD69 pKa = 3.44SIFDD73 pKa = 3.31VFEE76 pKa = 4.18QNNLSHH82 pKa = 6.33TASYY86 pKa = 10.14CQTGSSTGKK95 pKa = 10.08RR96 pKa = 11.84VLLGASGWSASGNCRR111 pKa = 11.84DD112 pKa = 4.2YY113 pKa = 11.6SDD115 pKa = 4.11SPEE118 pKa = 4.28GFSGLDD124 pKa = 3.5ALPDD128 pKa = 3.96FAGSDD133 pKa = 3.79APLTADD139 pKa = 5.79DD140 pKa = 5.16IVTFQNGNEE149 pKa = 3.98AARR152 pKa = 11.84TNLVQVPALGAFLGLPINIQDD173 pKa = 3.66GSGNPITNVDD183 pKa = 3.66LSTAEE188 pKa = 3.7VCAIFDD194 pKa = 3.69GSATVWSDD202 pKa = 2.93VGVPSSQQIKK212 pKa = 9.61VVHH215 pKa = 6.51RR216 pKa = 11.84SDD218 pKa = 3.96GSGTTFAFSQYY229 pKa = 10.6LAANCNSGLAPTFTTQEE246 pKa = 4.35LYY248 pKa = 11.13SDD250 pKa = 5.32AISLTVYY257 pKa = 10.32GGRR260 pKa = 11.84DD261 pKa = 3.22VGGSGNGGVIDD272 pKa = 4.92AVTGTPGAIGYY283 pKa = 10.9ANFSNVAVEE292 pKa = 4.26PGLAYY297 pKa = 10.53ALVEE301 pKa = 4.2GHH303 pKa = 7.11DD304 pKa = 3.92PANGGSINVAASDD317 pKa = 3.55ILIDD321 pKa = 5.43KK322 pKa = 10.54VLGDD326 pKa = 3.89NDD328 pKa = 4.44SVTGLPKK335 pKa = 10.53PEE337 pKa = 4.94DD338 pKa = 3.81VDD340 pKa = 4.11SSLAQEE346 pKa = 4.55CLAVVDD352 pKa = 4.47PAVQLEE358 pKa = 4.1QVYY361 pKa = 9.7PIVAFTNLLTYY372 pKa = 9.56TEE374 pKa = 4.59NNNDD378 pKa = 3.73PAATQALFTEE388 pKa = 5.15VTHH391 pKa = 6.3GTRR394 pKa = 11.84DD395 pKa = 3.49QNGEE399 pKa = 4.06PVYY402 pKa = 10.24DD403 pKa = 4.09LPVGYY408 pKa = 10.73ARR410 pKa = 11.84VASTAVSNIISTCINN425 pKa = 3.18

Molecular weight: 43.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K0CDP3|K0CDP3_ALCDB Molybdate ABC transporter periplasmic molybdate-binding protein OS=Alcanivorax dieselolei (strain DSM 16502 / CGMCC 1.3690 / B-5) OX=930169 GN=B5T_02429 PE=3 SV=1
MM1 pKa = 7.77ALPSSTKK8 pKa = 10.1SDD10 pKa = 3.6LLLVCVTLLAAVSWMFSKK28 pKa = 10.62EE29 pKa = 3.99AVLLMPPLLFMCLRR43 pKa = 11.84FLLAGLILALVGQRR57 pKa = 11.84QLRR60 pKa = 11.84RR61 pKa = 11.84MNRR64 pKa = 11.84DD65 pKa = 3.08KK66 pKa = 10.75YY67 pKa = 9.19WRR69 pKa = 11.84SIQVGMVFGAAMSFWIMGLHH89 pKa = 6.02YY90 pKa = 10.07GNHH93 pKa = 5.6VGEE96 pKa = 4.67GAFITSLGVVLVPVMARR113 pKa = 11.84LVFKK117 pKa = 8.46EE118 pKa = 4.24HH119 pKa = 7.09PPLSTWLALPVAVCGLALLSLDD141 pKa = 4.02GGFQGEE147 pKa = 4.4LGQLFFLASAMIFALFFTLNTRR169 pKa = 11.84AANNATITATGGRR182 pKa = 11.84TTTRR186 pKa = 11.84EE187 pKa = 3.87RR188 pKa = 11.84VPALALTSVVLVTVGLVTGTASLLLEE214 pKa = 4.2PWSPTFHH221 pKa = 7.36SGEE224 pKa = 4.21LGLVIAAWVIASATLGTAARR244 pKa = 11.84FFLQTYY250 pKa = 7.94AQSLSMHH257 pKa = 5.21SHH259 pKa = 5.87GVVIMILEE267 pKa = 4.25PMWTALLAALWFGEE281 pKa = 4.32TMNATQLGGCAMIFLALLVNRR302 pKa = 11.84WSAIRR307 pKa = 11.84RR308 pKa = 11.84VLKK311 pKa = 10.25RR312 pKa = 11.84WVDD315 pKa = 3.25QRR317 pKa = 11.84RR318 pKa = 11.84AARR321 pKa = 11.84AA322 pKa = 3.18

Molecular weight: 35.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4388

0

4388

1455496

37

4089

331.7

36.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.699 ± 0.049

0.918 ± 0.013

5.987 ± 0.036

6.053 ± 0.036

3.496 ± 0.024

8.457 ± 0.051

2.3 ± 0.021

4.375 ± 0.027

2.859 ± 0.033

11.229 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.42 ± 0.021

2.858 ± 0.024

5.054 ± 0.03

4.037 ± 0.026

7.332 ± 0.043

5.374 ± 0.03

5.072 ± 0.031

7.379 ± 0.034

1.522 ± 0.016

2.581 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski