Micavibrio aeruginosavorus (strain ARL-13)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Alphaproteobacteria incertae sedis; Micavibrio; Micavibrio aeruginosavorus

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2432 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G2KMQ4|G2KMQ4_MICAA Methyltransferase small domain protein OS=Micavibrio aeruginosavorus (strain ARL-13) OX=856793 GN=MICA_94 PE=4 SV=1
MM1 pKa = 7.54CAGAKK6 pKa = 9.75ASGMADD12 pKa = 3.21TVFTGTASGDD22 pKa = 3.62FMVMQGDD29 pKa = 4.28LQHH32 pKa = 5.78MTMTLTNPYY41 pKa = 9.19TGEE44 pKa = 4.08TVNINDD50 pKa = 3.55VYY52 pKa = 11.02KK53 pKa = 11.02VNNAAYY59 pKa = 9.43DD60 pKa = 3.9GLGGTDD66 pKa = 3.48ALLMSEE72 pKa = 4.8HH73 pKa = 7.33GDD75 pKa = 3.21MLLIKK80 pKa = 10.51DD81 pKa = 3.81ADD83 pKa = 3.71GDD85 pKa = 3.89QTLKK89 pKa = 11.01NIEE92 pKa = 4.05QIIAGDD98 pKa = 3.72GGDD101 pKa = 3.97VIVLADD107 pKa = 4.6AEE109 pKa = 4.27IQYY112 pKa = 11.32GNVAIDD118 pKa = 3.54GGGSGDD124 pKa = 4.95IIWSNNGNDD133 pKa = 4.08TIFALDD139 pKa = 3.74GNDD142 pKa = 3.17IVDD145 pKa = 4.02GGGGLDD151 pKa = 4.12KK152 pKa = 10.61IYY154 pKa = 10.82GGWGSDD160 pKa = 3.46YY161 pKa = 11.13LRR163 pKa = 11.84GGNGDD168 pKa = 4.17DD169 pKa = 3.93IIYY172 pKa = 10.49GGSRR176 pKa = 11.84SDD178 pKa = 3.59TEE180 pKa = 4.6AEE182 pKa = 4.05YY183 pKa = 8.5HH184 pKa = 5.64TVSTYY189 pKa = 10.92EE190 pKa = 3.72KK191 pKa = 9.61FFTVTHH197 pKa = 6.45SFPTLNEE204 pKa = 4.15GVQITPQQQAYY215 pKa = 9.74LGYY218 pKa = 9.3PSGEE222 pKa = 4.27TIVTFTTTATLKK234 pKa = 10.56FVSTDD239 pKa = 2.81AGYY242 pKa = 10.83KK243 pKa = 8.4NTVGVYY249 pKa = 8.97TVDD252 pKa = 3.11PDD254 pKa = 3.7GTIRR258 pKa = 11.84NADD261 pKa = 3.71FAFMNARR268 pKa = 11.84ATASGTSHH276 pKa = 6.12EE277 pKa = 4.8FEE279 pKa = 4.15VSGAVGVQIGLFMIANGYY297 pKa = 8.12NANGGYY303 pKa = 10.51GGIDD307 pKa = 3.59MNTGTLNFYY316 pKa = 10.88YY317 pKa = 10.45NYY319 pKa = 10.3GQADD323 pKa = 3.4QRR325 pKa = 11.84LAKK328 pKa = 9.73VTDD331 pKa = 4.25DD332 pKa = 4.04GDD334 pKa = 3.95NVSLIWSDD342 pKa = 3.09GTTDD346 pKa = 3.48KK347 pKa = 11.34LIAGTIYY354 pKa = 10.47HH355 pKa = 6.65SSASDD360 pKa = 3.46GSSSLNRR367 pKa = 11.84DD368 pKa = 3.58DD369 pKa = 5.06NIHH372 pKa = 6.52VITGLANAGDD382 pKa = 4.13NTTLRR387 pKa = 11.84LGFEE391 pKa = 4.64DD392 pKa = 4.49QDD394 pKa = 3.89HH395 pKa = 7.05LGDD398 pKa = 4.28ADD400 pKa = 4.13FNDD403 pKa = 4.37VIVDD407 pKa = 4.05LQVEE411 pKa = 4.5TQVVEE416 pKa = 4.77TILRR420 pKa = 11.84PDD422 pKa = 3.98DD423 pKa = 5.12DD424 pKa = 4.18ILYY427 pKa = 10.71GDD429 pKa = 4.85AGNDD433 pKa = 3.3TLYY436 pKa = 11.24GGFGNDD442 pKa = 3.37VLNGGTGSDD451 pKa = 3.7HH452 pKa = 7.41LYY454 pKa = 10.72GGQGADD460 pKa = 2.81TFVFDD465 pKa = 4.9NMDD468 pKa = 3.49GFVDD472 pKa = 4.17TVHH475 pKa = 7.16DD476 pKa = 5.25FKK478 pKa = 11.49ASEE481 pKa = 4.25GDD483 pKa = 3.54VLDD486 pKa = 4.41ISDD489 pKa = 4.14ILSGYY494 pKa = 9.71DD495 pKa = 3.64AGVDD499 pKa = 4.71DD500 pKa = 4.56ITDD503 pKa = 3.77FLKK506 pKa = 10.8FVQNGSNVDD515 pKa = 3.75LYY517 pKa = 11.96VNADD521 pKa = 3.54GDD523 pKa = 3.93SGGAFDD529 pKa = 4.47RR530 pKa = 11.84VAVIVGGVGGASVQDD545 pKa = 4.33LLDD548 pKa = 4.02GGAIVAAQTAVV559 pKa = 2.85

Molecular weight: 58.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G2KS99|G2KS99_MICAA HemY family protein OS=Micavibrio aeruginosavorus (strain ARL-13) OX=856793 GN=MICA_439 PE=4 SV=1
MM1 pKa = 7.17WRR3 pKa = 11.84AINARR8 pKa = 11.84HH9 pKa = 5.99IKK11 pKa = 9.85ILPIPKK17 pKa = 9.27RR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84KK21 pKa = 9.83SGPILLRR28 pKa = 11.84GSARR32 pKa = 11.84APPAVVV38 pKa = 2.92

Molecular weight: 4.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2432

0

2432

743786

37

2892

305.8

33.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.746 ± 0.059

0.917 ± 0.019

6.612 ± 0.051

5.285 ± 0.05

3.728 ± 0.036

8.038 ± 0.071

2.202 ± 0.025

5.862 ± 0.036

4.613 ± 0.055

8.955 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.214 ± 0.029

3.762 ± 0.049

4.824 ± 0.039

3.506 ± 0.028

5.825 ± 0.058

5.37 ± 0.047

5.84 ± 0.052

6.986 ± 0.043

1.116 ± 0.02

2.6 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski