Aptenodytes forsteri (Emperor penguin)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Sauropsida; Sauria; Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13704 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A087R909|A0A087R909_APTFO Uncharacterized protein (Fragment) OS=Aptenodytes forsteri OX=9233 GN=AS27_04805 PE=4 SV=1
RR1 pKa = 7.53LFWPKK6 pKa = 10.17SRR8 pKa = 11.84CYY10 pKa = 10.65DD11 pKa = 3.46YY12 pKa = 11.1LYY14 pKa = 10.94QEE16 pKa = 5.16AEE18 pKa = 4.0ALLKK22 pKa = 10.54NFPVQATISFYY33 pKa = 11.15DD34 pKa = 4.08DD35 pKa = 3.64SDD37 pKa = 4.77SEE39 pKa = 4.34DD40 pKa = 4.91DD41 pKa = 4.17EE42 pKa = 7.03DD43 pKa = 5.47EE44 pKa = 4.92LEE46 pKa = 4.01QDD48 pKa = 3.46SRR50 pKa = 11.84TEE52 pKa = 3.91SDD54 pKa = 3.48CC55 pKa = 4.64

Molecular weight: 6.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A087QHP3|A0A087QHP3_APTFO Uncharacterized protein (Fragment) OS=Aptenodytes forsteri OX=9233 GN=AS27_09823 PE=4 SV=1
KKK2 pKa = 10.08RR3 pKa = 11.84QGKKK7 pKa = 8.97RR8 pKa = 11.84QGKKK12 pKa = 8.73RR13 pKa = 11.84QGKKK17 pKa = 8.73RR18 pKa = 11.84QGKKK22 pKa = 8.73RR23 pKa = 11.84QGKKK27 pKa = 8.73RR28 pKa = 11.84QGKKK32 pKa = 8.73RR33 pKa = 11.84QGKKK37 pKa = 8.73RR38 pKa = 11.84QGKKK42 pKa = 8.73RR43 pKa = 11.84QGKKK47 pKa = 8.67RR48 pKa = 11.84QGGIRR53 pKa = 11.84RR54 pKa = 11.84HHH56 pKa = 5.41FQWT

Molecular weight: 6.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13704

0

13704

6135799

31

8772

447.7

50.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.468 ± 0.02

2.261 ± 0.02

5.03 ± 0.015

7.17 ± 0.025

3.9 ± 0.014

5.955 ± 0.026

2.537 ± 0.01

4.951 ± 0.016

6.409 ± 0.02

9.727 ± 0.031

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.233 ± 0.009

4.116 ± 0.015

5.303 ± 0.03

4.672 ± 0.021

5.275 ± 0.015

8.151 ± 0.029

5.362 ± 0.013

6.29 ± 0.017

1.197 ± 0.009

2.993 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski