Streptomyces phaeoluteigriseus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; Streptomyces aurantiacus group

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6311 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1V6MYH3|A0A1V6MYH3_9ACTN Uncharacterized protein OS=Streptomyces phaeoluteigriseus OX=114686 GN=BM536_002990 PE=4 SV=1
MM1 pKa = 7.68LLGDD5 pKa = 4.17PVGPSAVAVPYY16 pKa = 10.39AGPAVGGVPDD26 pKa = 5.29LPNFDD31 pKa = 4.63PARR34 pKa = 11.84SDD36 pKa = 3.34PAAVSGSPGEE46 pKa = 4.89AMDD49 pKa = 3.26VWRR52 pKa = 11.84QHH54 pKa = 5.53TGVNACAIGAQGMVLSHH71 pKa = 6.53LTGQEE76 pKa = 3.92YY77 pKa = 10.62SEE79 pKa = 4.26EE80 pKa = 4.08QLTQEE85 pKa = 4.38AASMGWFDD93 pKa = 4.98GSGTAPADD101 pKa = 3.38VGNLLEE107 pKa = 4.16HH108 pKa = 6.81HH109 pKa = 6.75GVPVQHH115 pKa = 6.58VEE117 pKa = 4.23GASLEE122 pKa = 4.06QLEE125 pKa = 4.31QVVASGGAAIVGVDD139 pKa = 3.98SSEE142 pKa = 3.77IWTSGYY148 pKa = 10.99DD149 pKa = 3.61PADD152 pKa = 4.29DD153 pKa = 4.24PVGTAPGIPGQDD165 pKa = 2.9ADD167 pKa = 3.75HH168 pKa = 6.5AVWVTGIDD176 pKa = 3.72YY177 pKa = 10.64SDD179 pKa = 4.19PASPMVILNDD189 pKa = 3.53SGHH192 pKa = 7.34PDD194 pKa = 3.51GQGLAVPLDD203 pKa = 3.88EE204 pKa = 6.73FMGAWQDD211 pKa = 3.57SGNLLVAAGAPGTVAPP227 pKa = 4.86

Molecular weight: 22.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1V6MLT5|A0A1V6MLT5_9ACTN Flavoprotein OS=Streptomyces phaeoluteigriseus OX=114686 GN=BM536_029490 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILANRR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.89GRR40 pKa = 11.84ANLSAA45 pKa = 4.66

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6311

0

6311

1882047

18

2622

298.2

32.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.245 ± 0.041

0.797 ± 0.01

6.096 ± 0.026

5.779 ± 0.032

2.769 ± 0.018

9.369 ± 0.03

2.331 ± 0.015

3.121 ± 0.023

2.188 ± 0.026

10.234 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.747 ± 0.014

1.788 ± 0.017

6.003 ± 0.029

2.75 ± 0.018

8.15 ± 0.04

5.106 ± 0.025

6.255 ± 0.026

8.611 ± 0.031

1.528 ± 0.013

2.133 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski