Gemmiger formicilis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Eubacteriales incertae sedis; Gemmiger

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2819 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1T4X083|A0A1T4X083_9FIRM Predicted transcriptional regulator OS=Gemmiger formicilis OX=745368 GN=SAMN02745178_01222 PE=3 SV=1
MM1 pKa = 7.49KK2 pKa = 10.22LKK4 pKa = 10.57NFRR7 pKa = 11.84RR8 pKa = 11.84VAALGMAVGMTAVLAACGNTADD30 pKa = 4.25TASSEE35 pKa = 4.28AAEE38 pKa = 4.52STAEE42 pKa = 4.04TADD45 pKa = 3.63TAEE48 pKa = 4.22EE49 pKa = 4.16ASAEE53 pKa = 4.25TADD56 pKa = 4.76AEE58 pKa = 4.43YY59 pKa = 10.3AYY61 pKa = 10.92LADD64 pKa = 5.2FSFSDD69 pKa = 3.63AFDD72 pKa = 3.6EE73 pKa = 4.2NGYY76 pKa = 10.6LKK78 pKa = 10.8GVTAADD84 pKa = 4.18YY85 pKa = 8.63VTLPDD90 pKa = 4.93DD91 pKa = 3.95YY92 pKa = 12.13ADD94 pKa = 3.47ITISADD100 pKa = 3.12LGQVSDD106 pKa = 5.55DD107 pKa = 5.35DD108 pKa = 4.2INDD111 pKa = 4.21YY112 pKa = 10.91IDD114 pKa = 4.99SNVLSNFATTNEE126 pKa = 4.19VKK128 pKa = 10.66DD129 pKa = 3.7RR130 pKa = 11.84AAADD134 pKa = 3.66GDD136 pKa = 4.41TVNIDD141 pKa = 3.19YY142 pKa = 10.68VGRR145 pKa = 11.84IDD147 pKa = 5.35GVAFDD152 pKa = 4.87GGDD155 pKa = 3.6TKK157 pKa = 11.39GSGADD162 pKa = 3.45LTLGSGTYY170 pKa = 9.51IDD172 pKa = 4.39NFEE175 pKa = 4.13DD176 pKa = 3.71QIVGHH181 pKa = 5.89TPGEE185 pKa = 4.4TFDD188 pKa = 3.66VTVTFPEE195 pKa = 4.91DD196 pKa = 3.56YY197 pKa = 11.23GNEE200 pKa = 3.96DD201 pKa = 3.92LNGKK205 pKa = 8.37EE206 pKa = 4.38AVFEE210 pKa = 4.27TTLNYY215 pKa = 9.8IKK217 pKa = 10.64EE218 pKa = 4.49SVTPEE223 pKa = 4.03LTDD226 pKa = 3.37DD227 pKa = 3.51WVKK230 pKa = 11.23EE231 pKa = 3.95NLGKK235 pKa = 10.87SMSLNSVDD243 pKa = 3.52EE244 pKa = 4.3LKK246 pKa = 11.21AFVKK250 pKa = 9.79STMLYY255 pKa = 10.59DD256 pKa = 3.66NQASDD261 pKa = 4.32VYY263 pKa = 10.51TALHH267 pKa = 6.85DD268 pKa = 3.85KK269 pKa = 10.96ASYY272 pKa = 10.98ADD274 pKa = 4.5EE275 pKa = 4.72LPQTALDD282 pKa = 3.86YY283 pKa = 11.35YY284 pKa = 11.07RR285 pKa = 11.84DD286 pKa = 3.53VLLYY290 pKa = 10.41RR291 pKa = 11.84IYY293 pKa = 11.12TNAQKK298 pKa = 10.98YY299 pKa = 6.83GTDD302 pKa = 3.38MNALLSSGMMGSSYY316 pKa = 11.3DD317 pKa = 3.75SVDD320 pKa = 4.17AYY322 pKa = 11.24LADD325 pKa = 4.35ANDD328 pKa = 4.27SIKK331 pKa = 10.98SITQQALLMQAVAEE345 pKa = 4.23KK346 pKa = 10.13TGFKK350 pKa = 10.49CDD352 pKa = 3.6TATMNADD359 pKa = 4.03FGRR362 pKa = 11.84YY363 pKa = 9.16YY364 pKa = 10.6GTTDD368 pKa = 3.32PSQYY372 pKa = 10.13VSAYY376 pKa = 8.17GEE378 pKa = 4.36NYY380 pKa = 9.82IKK382 pKa = 10.64MNVLQSDD389 pKa = 3.79VMQNLIDD396 pKa = 3.82NVKK399 pKa = 10.59YY400 pKa = 9.98EE401 pKa = 3.96

Molecular weight: 43.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1T4XSI1|A0A1T4XSI1_9FIRM Uncharacterized protein OS=Gemmiger formicilis OX=745368 GN=SAMN02745178_02227 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.6RR3 pKa = 11.84TFQPKK8 pKa = 8.37KK9 pKa = 7.49RR10 pKa = 11.84QRR12 pKa = 11.84SRR14 pKa = 11.84VHH16 pKa = 6.24GFLNRR21 pKa = 11.84MATKK25 pKa = 10.47NGRR28 pKa = 11.84KK29 pKa = 9.36VIARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.98GRR39 pKa = 11.84KK40 pKa = 8.89SLTVV44 pKa = 3.12

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2819

0

2819

923584

39

3580

327.6

36.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.668 ± 0.071

1.735 ± 0.021

5.86 ± 0.037

5.946 ± 0.048

3.773 ± 0.035

7.517 ± 0.047

1.919 ± 0.02

5.32 ± 0.043

4.844 ± 0.044

9.864 ± 0.068

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.666 ± 0.026

3.593 ± 0.038

4.335 ± 0.035

3.515 ± 0.029

5.305 ± 0.045

5.12 ± 0.037

6.099 ± 0.052

7.011 ± 0.04

1.142 ± 0.018

3.766 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski