Auritidibacter sp. NML120636

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Micrococcaceae; Auritidibacter; unclassified Auritidibacter

Average proteome isoelectric point is 5.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2118 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A318AH37|A0A318AH37_9MICC C4-dicarboxylate ABC transporter substrate-binding protein OS=Auritidibacter sp. NML120636 OX=2170743 GN=DCC25_09155 PE=4 SV=1
MM1 pKa = 7.66ALTGSHH7 pKa = 6.77HH8 pKa = 5.89EE9 pKa = 4.24HH10 pKa = 6.83SLTTSLTRR18 pKa = 11.84YY19 pKa = 9.03FVGFATLLLLLTGCAGSGDD38 pKa = 4.15PEE40 pKa = 4.39AADD43 pKa = 3.59TSSIPGTDD51 pKa = 3.22TQDD54 pKa = 3.25APEE57 pKa = 4.99PPTEE61 pKa = 4.97AEE63 pKa = 4.68DD64 pKa = 3.81SSSAPDD70 pKa = 3.59TPSTEE75 pKa = 4.36EE76 pKa = 3.99SSEE79 pKa = 4.09EE80 pKa = 3.9PPEE83 pKa = 4.04PTEE86 pKa = 4.07PEE88 pKa = 3.99IEE90 pKa = 4.29SLEE93 pKa = 4.21DD94 pKa = 3.28TDD96 pKa = 4.83FSSISFQFVGYY107 pKa = 10.48SDD109 pKa = 3.93TDD111 pKa = 3.52DD112 pKa = 4.93SEE114 pKa = 4.76EE115 pKa = 3.97KK116 pKa = 10.22TFVDD120 pKa = 4.21GTVEE124 pKa = 4.04AGDD127 pKa = 3.53PQFPVTYY134 pKa = 9.56EE135 pKa = 3.79LGEE138 pKa = 4.6VIHH141 pKa = 6.71QDD143 pKa = 3.38LNGDD147 pKa = 4.37GIVDD151 pKa = 3.58AAVEE155 pKa = 3.95IEE157 pKa = 4.16RR158 pKa = 11.84YY159 pKa = 9.32EE160 pKa = 4.23GNSYY164 pKa = 10.82DD165 pKa = 3.64GRR167 pKa = 11.84WYY169 pKa = 9.53MWIATEE175 pKa = 5.14DD176 pKa = 3.94GPEE179 pKa = 3.92QSPLPFARR187 pKa = 11.84SGNCGDD193 pKa = 3.54QVEE196 pKa = 5.03SVTATNDD203 pKa = 3.12GLEE206 pKa = 4.21VHH208 pKa = 5.96EE209 pKa = 4.62HH210 pKa = 5.66LRR212 pKa = 11.84AKK214 pKa = 10.44EE215 pKa = 3.98DD216 pKa = 3.67DD217 pKa = 4.05VSCAEE222 pKa = 4.4HH223 pKa = 6.56GLHH226 pKa = 6.31EE227 pKa = 4.36RR228 pKa = 11.84TRR230 pKa = 11.84TIAADD235 pKa = 3.69YY236 pKa = 10.61FDD238 pKa = 5.88LAGAWWPVQVAPVIGYY254 pKa = 10.11GGICSLTGDD263 pKa = 4.19EE264 pKa = 5.6GMTAPVDD271 pKa = 3.48IAHH274 pKa = 5.88VNPYY278 pKa = 9.93KK279 pKa = 10.57EE280 pKa = 4.26SASIAEE286 pKa = 4.66TNAEE290 pKa = 4.19THH292 pKa = 6.83PEE294 pKa = 3.95LTILFPFGEE303 pKa = 4.79GDD305 pKa = 3.43GSDD308 pKa = 3.22RR309 pKa = 11.84KK310 pKa = 10.01IDD312 pKa = 3.84GEE314 pKa = 3.81WWAIGGIFDD323 pKa = 4.22GSQVLGCGWVAYY335 pKa = 9.38EE336 pKa = 4.92DD337 pKa = 4.09LPIGIRR343 pKa = 11.84FGG345 pKa = 3.44

Molecular weight: 37.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A318AGA3|A0A318AGA3_9MICC Uncharacterized protein OS=Auritidibacter sp. NML120636 OX=2170743 GN=DCC25_00390 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84ARR15 pKa = 11.84KK16 pKa = 8.24HH17 pKa = 4.59GFRR20 pKa = 11.84ARR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AVLSARR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.31GRR40 pKa = 11.84ARR42 pKa = 11.84VSAA45 pKa = 4.0

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2118

0

2118

715878

31

2147

338.0

36.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.97 ± 0.052

0.593 ± 0.013

6.149 ± 0.045

6.377 ± 0.049

3.087 ± 0.031

8.009 ± 0.045

2.558 ± 0.029

4.907 ± 0.035

2.515 ± 0.042

9.797 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.115 ± 0.024

2.577 ± 0.023

5.214 ± 0.035

4.21 ± 0.035

6.348 ± 0.051

6.11 ± 0.042

6.627 ± 0.037

8.199 ± 0.046

1.408 ± 0.022

2.23 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski