Roseimaritima ulvae
Average proteome isoelectric point is 6.12
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5779 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5B9QM57|A0A5B9QM57_9BACT Sensory transduction protein regX3 OS=Roseimaritima ulvae OX=980254 GN=regX3_2 PE=4 SV=1
MM1 pKa = 7.22 PTLPRR6 pKa = 11.84 HH7 pKa = 5.52 RR8 pKa = 11.84 RR9 pKa = 11.84 SLRR12 pKa = 11.84 CEE14 pKa = 3.63 SLEE17 pKa = 4.43 RR18 pKa = 11.84 RR19 pKa = 11.84 NLLAAGFGLEE29 pKa = 4.54 HH30 pKa = 6.71 NFISPEE36 pKa = 4.01 DD37 pKa = 3.76 VDD39 pKa = 4.32 GSGDD43 pKa = 3.57 VTPYY47 pKa = 10.96 DD48 pKa = 3.51 ALQVVNNLNRR58 pKa = 11.84 QARR61 pKa = 11.84 SEE63 pKa = 4.14 GQGEE67 pKa = 4.38 DD68 pKa = 3.47 SSSSSGLMLDD78 pKa = 3.62 VDD80 pKa = 4.48 ADD82 pKa = 4.24 GTSSPSDD89 pKa = 2.57 AWRR92 pKa = 11.84 IINYY96 pKa = 9.39 LNQNGVHH103 pKa = 6.27 GALASSLVPIHH114 pKa = 7.02 DD115 pKa = 5.01 RR116 pKa = 11.84 IAALEE121 pKa = 4.19 SALHH125 pKa = 5.96 SSSVPSWLGSGTAHH139 pKa = 7.95 DD140 pKa = 4.73 ILASLYY146 pKa = 10.79 NGIAPEE152 pKa = 4.27 INSDD156 pKa = 4.04 LQDD159 pKa = 3.92 EE160 pKa = 4.88 LDD162 pKa = 4.05 DD163 pKa = 4.22 ALEE166 pKa = 4.34 RR167 pKa = 11.84 FDD169 pKa = 6.22 LQLQYY174 pKa = 11.57 NEE176 pKa = 4.4 LSDD179 pKa = 4.16 RR180 pKa = 11.84 LDD182 pKa = 4.2 SIADD186 pKa = 3.89 DD187 pKa = 4.48 LPSFDD192 pKa = 6.0 SIFSQLGSQIGSQYY206 pKa = 10.99 HH207 pKa = 5.42 EE208 pKa = 4.84 LQDD211 pKa = 3.78 EE212 pKa = 4.32 LADD215 pKa = 4.05 ALEE218 pKa = 4.36 EE219 pKa = 4.06 LHH221 pKa = 7.07 DD222 pKa = 3.95 QAGSVLDD229 pKa = 3.98 EE230 pKa = 5.08 LFDD233 pKa = 3.64 DD234 pKa = 5.45 HH235 pKa = 8.9 YY236 pKa = 11.8 YY237 pKa = 11.29 DD238 pKa = 6.1 DD239 pKa = 6.18 DD240 pKa = 5.03 SDD242 pKa = 4.32 EE243 pKa = 4.24 NTEE246 pKa = 4.34 SVNGLAEE253 pKa = 4.46 NGLSDD258 pKa = 5.83 DD259 pKa = 5.07 DD260 pKa = 5.37 DD261 pKa = 5.44 SSTTEE266 pKa = 3.68 QVSDD270 pKa = 3.73 VLSHH274 pKa = 7.44 IDD276 pKa = 3.7 EE277 pKa = 4.31 EE278 pKa = 4.91 LEE280 pKa = 3.84 NLFYY284 pKa = 11.16 DD285 pKa = 4.16 VASDD289 pKa = 3.13 IHH291 pKa = 6.9 DD292 pKa = 4.29 LNFSGVLNTLANVVHH307 pKa = 5.66 VHH309 pKa = 6.61 LPLLSSGLPDD319 pKa = 4.74 LFDD322 pKa = 5.08 SNIVDD327 pKa = 3.54 IPNDD331 pKa = 3.61 FSSEE335 pKa = 3.91 LSDD338 pKa = 4.39 LYY340 pKa = 11.45 DD341 pKa = 3.83 RR342 pKa = 11.84 LEE344 pKa = 3.98 SVYY347 pKa = 11.2 DD348 pKa = 3.65 DD349 pKa = 3.58 VVPEE353 pKa = 4.19 VEE355 pKa = 4.59 DD356 pKa = 4.21 ALDD359 pKa = 3.46 HH360 pKa = 6.41 VFDD363 pKa = 3.87 SFSYY367 pKa = 9.25 IGSAASHH374 pKa = 6.64 LSSFVHH380 pKa = 5.81 YY381 pKa = 9.97 LHH383 pKa = 7.61 GYY385 pKa = 8.54 GSDD388 pKa = 3.65 YY389 pKa = 10.78 NTATT393 pKa = 3.68
Molecular weight: 43.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.685
IPC2_protein 3.783
IPC_protein 3.821
Toseland 3.592
ProMoST 3.986
Dawson 3.808
Bjellqvist 3.961
Wikipedia 3.757
Rodwell 3.643
Grimsley 3.503
Solomon 3.808
Lehninger 3.77
Nozaki 3.923
DTASelect 4.19
Thurlkill 3.643
EMBOSS 3.757
Sillero 3.935
Patrickios 1.43
IPC_peptide 3.808
IPC2_peptide 3.923
IPC2.peptide.svr19 3.844
Protein with the highest isoelectric point:
>tr|A0A5B9QKU1|A0A5B9QKU1_9BACT 50S ribosomal protein L7/L12 OS=Roseimaritima ulvae OX=980254 GN=rplL_1 PE=3 SV=1
MM1 pKa = 7.1 VRR3 pKa = 11.84 LSRR6 pKa = 11.84 LTRR9 pKa = 11.84 SRR11 pKa = 11.84 RR12 pKa = 11.84 SLKK15 pKa = 10.24 KK16 pKa = 10.48 FRR18 pKa = 11.84 THH20 pKa = 4.99 PHH22 pKa = 5.91 ARR24 pKa = 11.84 QAPQAQAWSAGRR36 pKa = 11.84 SQSKK40 pKa = 10.44 PNGLEE45 pKa = 3.92 KK46 pKa = 10.8 QKK48 pKa = 9.96 TNPTLRR54 pKa = 11.84 GSPSRR59 pKa = 11.84 RR60 pKa = 11.84 LRR62 pKa = 11.84 LRR64 pKa = 11.84 VKK66 pKa = 10.34 RR67 pKa = 11.84 GSLRR71 pKa = 11.84 SLKK74 pKa = 10.32 KK75 pKa = 10.54 FRR77 pKa = 11.84 THH79 pKa = 4.99 PHH81 pKa = 5.91 ARR83 pKa = 11.84 QAPQAQAWSAGRR95 pKa = 11.84 SQSKK99 pKa = 10.44 PNGLEE104 pKa = 3.92 KK105 pKa = 10.8 QKK107 pKa = 9.96 TNPTLRR113 pKa = 11.84 GSPRR117 pKa = 11.84 RR118 pKa = 11.84 RR119 pKa = 11.84 LRR121 pKa = 11.84 LRR123 pKa = 11.84 VKK125 pKa = 9.95 RR126 pKa = 11.84 GSRR129 pKa = 11.84 RR130 pKa = 11.84 SLKK133 pKa = 10.27 KK134 pKa = 10.52 FRR136 pKa = 11.84 THH138 pKa = 4.99 PHH140 pKa = 5.91 ARR142 pKa = 11.84 QAPQAQAWSAGRR154 pKa = 11.84 SQSKK158 pKa = 10.44 PNGLEE163 pKa = 3.92 KK164 pKa = 10.8 QKK166 pKa = 9.96 TNPTLRR172 pKa = 11.84 GSPRR176 pKa = 11.84 RR177 pKa = 11.84 RR178 pKa = 11.84 LRR180 pKa = 11.84 LRR182 pKa = 11.84 VKK184 pKa = 9.95 RR185 pKa = 11.84 GSRR188 pKa = 11.84 RR189 pKa = 11.84 SLKK192 pKa = 10.27 KK193 pKa = 10.52 FRR195 pKa = 11.84 THH197 pKa = 4.99 PHH199 pKa = 5.91 ARR201 pKa = 11.84 QAPQAQAWSAGRR213 pKa = 11.84 SQSKK217 pKa = 10.44 PNGLEE222 pKa = 3.92 KK223 pKa = 10.8 QKK225 pKa = 9.96 TNPTLRR231 pKa = 11.84 GSPRR235 pKa = 11.84 RR236 pKa = 11.84 RR237 pKa = 11.84 LRR239 pKa = 11.84 LRR241 pKa = 11.84 VKK243 pKa = 9.93 RR244 pKa = 11.84 DD245 pKa = 2.99 SRR247 pKa = 11.84 RR248 pKa = 11.84 SLKK251 pKa = 10.4 KK252 pKa = 10.21 FHH254 pKa = 5.99 THH256 pKa = 4.19 PHH258 pKa = 5.8 ARR260 pKa = 11.84 QAPQAQAWSAGRR272 pKa = 11.84 SQSKK276 pKa = 10.44 PNGLEE281 pKa = 3.92 KK282 pKa = 10.8 QKK284 pKa = 9.96 TNPTLRR290 pKa = 11.84 GSPRR294 pKa = 11.84 RR295 pKa = 11.84 RR296 pKa = 11.84 LRR298 pKa = 11.84 LRR300 pKa = 11.84 VKK302 pKa = 9.95 RR303 pKa = 11.84 GSRR306 pKa = 11.84 RR307 pKa = 11.84 SLKK310 pKa = 10.31 KK311 pKa = 10.36 FHH313 pKa = 5.99 THH315 pKa = 4.19 PHH317 pKa = 5.8 ARR319 pKa = 11.84 QAPQAQAWSGSVVV332 pKa = 2.92
Molecular weight: 38.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.508
IPC2_protein 11.155
IPC_protein 12.749
Toseland 12.91
ProMoST 13.408
Dawson 12.91
Bjellqvist 12.91
Wikipedia 13.393
Rodwell 12.603
Grimsley 12.954
Solomon 13.408
Lehninger 13.305
Nozaki 12.91
DTASelect 12.91
Thurlkill 12.91
EMBOSS 13.408
Sillero 12.91
Patrickios 12.31
IPC_peptide 13.408
IPC2_peptide 12.398
IPC2.peptide.svr19 9.142
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5779
0
5779
2373878
29
9958
410.8
45.1
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.552 ± 0.04
1.1 ± 0.015
6.352 ± 0.042
5.85 ± 0.034
3.482 ± 0.02
7.657 ± 0.046
2.235 ± 0.019
4.541 ± 0.019
2.999 ± 0.033
10.052 ± 0.043
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.147 ± 0.019
2.962 ± 0.032
5.522 ± 0.023
4.772 ± 0.029
6.866 ± 0.05
6.19 ± 0.036
5.599 ± 0.046
7.213 ± 0.028
1.519 ± 0.015
2.389 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here