Gemycircularvirus sp.

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; Gemycircularvirus; unclassified Gemycircularvirus

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2U3T953|A0A2U3T953_9VIRU Capsid OS=Gemycircularvirus sp. OX=1983771 GN=Cap PE=4 SV=1
MM1 pKa = 7.34TFILNARR8 pKa = 11.84YY9 pKa = 8.98FLVTYY14 pKa = 7.07PQCDD18 pKa = 3.66GLDD21 pKa = 3.2EE22 pKa = 4.42WSVNDD27 pKa = 3.83HH28 pKa = 6.42FGSLGAEE35 pKa = 4.02CIVARR40 pKa = 11.84EE41 pKa = 4.04DD42 pKa = 3.46HH43 pKa = 6.99ADD45 pKa = 3.7GGTHH49 pKa = 6.54LHH51 pKa = 5.76VFCDD55 pKa = 4.88FGRR58 pKa = 11.84KK59 pKa = 8.2FRR61 pKa = 11.84SRR63 pKa = 11.84RR64 pKa = 11.84ADD66 pKa = 3.04IFDD69 pKa = 3.3VGGFHH74 pKa = 7.62PNIEE78 pKa = 4.01RR79 pKa = 11.84SKK81 pKa = 10.18KK82 pKa = 9.89NPRR85 pKa = 11.84KK86 pKa = 9.57GAAYY90 pKa = 9.66ACKK93 pKa = 10.55DD94 pKa = 3.2GDD96 pKa = 4.08VVAGGLAIPQLPTRR110 pKa = 11.84LVSGAQDD117 pKa = 3.46PGSTLVCAEE126 pKa = 4.1SQRR129 pKa = 11.84EE130 pKa = 4.05FFDD133 pKa = 3.47VAEE136 pKa = 4.29DD137 pKa = 4.97LITKK141 pKa = 9.94FGSFYY146 pKa = 11.46AFARR150 pKa = 11.84WKK152 pKa = 10.03WPEE155 pKa = 3.49LDD157 pKa = 4.28KK158 pKa = 11.31SYY160 pKa = 10.85EE161 pKa = 4.21SPPGLTITDD170 pKa = 3.71GAFPEE175 pKa = 4.23LVRR178 pKa = 11.84YY179 pKa = 7.65RR180 pKa = 11.84TEE182 pKa = 3.78VMGNRR187 pKa = 11.84VNTLCVYY194 pKa = 10.78GEE196 pKa = 4.28SLTGKK201 pKa = 5.81TTWARR206 pKa = 11.84ALGSHH211 pKa = 7.46LYY213 pKa = 9.32MKK215 pKa = 10.74ARR217 pKa = 11.84FNARR221 pKa = 11.84SAMLAEE227 pKa = 4.23GVEE230 pKa = 4.23YY231 pKa = 10.76AVIDD235 pKa = 4.97DD236 pKa = 3.7ISGGIAYY243 pKa = 9.13FPHH246 pKa = 6.21FKK248 pKa = 10.34DD249 pKa = 3.2WFGGQPDD256 pKa = 3.8VQVRR260 pKa = 11.84ALYY263 pKa = 10.51KK264 pKa = 10.64DD265 pKa = 4.06DD266 pKa = 4.41VLLRR270 pKa = 11.84WGKK273 pKa = 7.33PTIWLNQRR281 pKa = 11.84DD282 pKa = 4.25PRR284 pKa = 11.84DD285 pKa = 3.41QLRR288 pKa = 11.84DD289 pKa = 3.57MQGPKK294 pKa = 10.44YY295 pKa = 10.33STDD298 pKa = 3.22QYY300 pKa = 11.19EE301 pKa = 4.84SDD303 pKa = 4.91VKK305 pKa = 10.7WLDD308 pKa = 3.56DD309 pKa = 3.57NCIFVYY315 pKa = 10.26VDD317 pKa = 3.33SPIVTFRR324 pKa = 11.84ASTEE328 pKa = 3.77

Molecular weight: 37.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2U3T957|A0A2U3T957_9VIRU RepA OS=Gemycircularvirus sp. OX=1983771 GN=RepA PE=4 SV=1
MM1 pKa = 7.97AYY3 pKa = 9.49RR4 pKa = 11.84RR5 pKa = 11.84KK6 pKa = 9.53YY7 pKa = 8.93SSYY10 pKa = 9.73RR11 pKa = 11.84ARR13 pKa = 11.84RR14 pKa = 11.84PTKK17 pKa = 10.0RR18 pKa = 11.84YY19 pKa = 5.1GTRR22 pKa = 11.84GAGKK26 pKa = 9.92RR27 pKa = 11.84KK28 pKa = 9.5YY29 pKa = 9.95GSKK32 pKa = 9.82KK33 pKa = 9.39RR34 pKa = 11.84SYY36 pKa = 10.29RR37 pKa = 11.84KK38 pKa = 9.8NPAMSKK44 pKa = 10.29KK45 pKa = 10.31RR46 pKa = 11.84ILNVTSRR53 pKa = 11.84KK54 pKa = 9.6KK55 pKa = 10.5KK56 pKa = 7.97NTMMQYY62 pKa = 11.09VNTDD66 pKa = 3.07STAGTSVSIGPGPMLVSGNSQYY88 pKa = 10.81WGVFTPTAMDD98 pKa = 3.84LTSGADD104 pKa = 3.62LQNSIVDD111 pKa = 3.56SSLRR115 pKa = 11.84TSQTCFIRR123 pKa = 11.84GLRR126 pKa = 11.84EE127 pKa = 3.97NIHH130 pKa = 6.75IEE132 pKa = 4.15TSTGLPWYY140 pKa = 8.09WRR142 pKa = 11.84RR143 pKa = 11.84IVFTAKK149 pKa = 8.65STPWFSFSPNDD160 pKa = 3.76TPTQTNSSSTSAIEE174 pKa = 4.17SNTNGWEE181 pKa = 3.56RR182 pKa = 11.84LYY184 pKa = 11.06FNQYY188 pKa = 9.01VNNAPDD194 pKa = 4.15TISRR198 pKa = 11.84MQRR201 pKa = 11.84DD202 pKa = 4.27LFRR205 pKa = 11.84GEE207 pKa = 4.19QNRR210 pKa = 11.84DD211 pKa = 2.45WSDD214 pKa = 2.85ILTAPIDD221 pKa = 3.68TARR224 pKa = 11.84VDD226 pKa = 3.91LKK228 pKa = 10.92SDD230 pKa = 3.35RR231 pKa = 11.84LRR233 pKa = 11.84VIKK236 pKa = 10.55SGNQVGTNRR245 pKa = 11.84AFKK248 pKa = 10.2MFYY251 pKa = 9.93PCNKK255 pKa = 9.56NLTYY259 pKa = 10.89NDD261 pKa = 4.26DD262 pKa = 3.48EE263 pKa = 5.42SGVVEE268 pKa = 4.21GTTFSSVQDD277 pKa = 3.36KK278 pKa = 11.07RR279 pKa = 11.84GMGNLHH285 pKa = 6.09VVDD288 pKa = 4.62IFLPGTGGAKK298 pKa = 10.25GDD300 pKa = 3.81ILKK303 pKa = 9.03LTSTSTLYY311 pKa = 9.69WHH313 pKa = 7.1EE314 pKa = 4.2KK315 pKa = 9.32

Molecular weight: 35.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

835

192

328

278.3

31.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.629 ± 1.085

1.078 ± 0.503

6.467 ± 1.126

4.551 ± 1.002

4.79 ± 0.696

7.186 ± 0.892

1.557 ± 0.315

3.952 ± 0.269

4.91 ± 0.864

8.862 ± 2.264

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.874 ± 0.752

4.311 ± 1.102

5.389 ± 0.863

2.874 ± 0.228

7.545 ± 0.472

8.982 ± 1.649

7.665 ± 1.328

5.629 ± 0.661

2.395 ± 0.083

3.353 ± 0.956
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski