Rat cytomegalovirus (strain Maastricht)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes; Herpesvirales; Herpesviridae; Betaherpesvirinae; Muromegalovirus; Murid betaherpesvirus 2

Average proteome isoelectric point is 7.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 167 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q9DWH6|Q9DWH6_RCMVM Pr3 OS=Rat cytomegalovirus (strain Maastricht) OX=79700 GN=r3 PE=4 SV=1
MM1 pKa = 7.33VPPVDD6 pKa = 3.72TVNGRR11 pKa = 11.84EE12 pKa = 4.05DD13 pKa = 3.16GTLRR17 pKa = 11.84FVFSARR23 pKa = 11.84RR24 pKa = 11.84SGVYY28 pKa = 9.85HH29 pKa = 6.87GLLSLDD35 pKa = 3.55VVKK38 pKa = 10.81KK39 pKa = 8.33FSCVMTVPADD49 pKa = 3.45PCLGARR55 pKa = 11.84QTAAFPSAAAASLPVATTTAEE76 pKa = 3.74NGTRR80 pKa = 11.84PRR82 pKa = 11.84PAGPAPGPPAGGGHH96 pKa = 7.09LDD98 pKa = 3.76VYY100 pKa = 10.97SSTVVFVILSLVLLGGLVMLSVCIWGSSKK129 pKa = 10.93GADD132 pKa = 3.25NGIVLAGDD140 pKa = 3.38SHH142 pKa = 8.41GEE144 pKa = 3.89DD145 pKa = 3.46DD146 pKa = 4.36RR147 pKa = 11.84EE148 pKa = 4.31TVVEE152 pKa = 4.4VVSDD156 pKa = 3.81DD157 pKa = 4.45SEE159 pKa = 5.05VEE161 pKa = 4.22GPDD164 pKa = 3.21VAGDD168 pKa = 3.48VGGDD172 pKa = 3.19RR173 pKa = 11.84RR174 pKa = 11.84DD175 pKa = 3.52DD176 pKa = 4.3DD177 pKa = 4.14GASEE181 pKa = 4.16GKK183 pKa = 10.34EE184 pKa = 3.85EE185 pKa = 4.25AEE187 pKa = 3.99EE188 pKa = 3.99EE189 pKa = 4.27AEE191 pKa = 4.17EE192 pKa = 4.1EE193 pKa = 4.24AEE195 pKa = 4.17EE196 pKa = 3.99EE197 pKa = 4.21AEE199 pKa = 3.96EE200 pKa = 4.26EE201 pKa = 3.97EE202 pKa = 4.42RR203 pKa = 11.84EE204 pKa = 3.95RR205 pKa = 11.84RR206 pKa = 11.84GRR208 pKa = 11.84EE209 pKa = 3.72VEE211 pKa = 3.83PDD213 pKa = 3.7GEE215 pKa = 4.13VDD217 pKa = 2.48PWGRR221 pKa = 11.84FRR223 pKa = 11.84RR224 pKa = 11.84EE225 pKa = 3.6DD226 pKa = 2.98WPYY229 pKa = 10.47PPRR232 pKa = 11.84APPSTSDD239 pKa = 3.28EE240 pKa = 4.24SDD242 pKa = 3.49DD243 pKa = 5.34ARR245 pKa = 11.84DD246 pKa = 3.54SDD248 pKa = 4.33GAAGDD253 pKa = 4.43DD254 pKa = 3.77SSGVCDD260 pKa = 5.91LSTEE264 pKa = 4.17DD265 pKa = 3.93LRR267 pKa = 11.84GTVSVVVTISAVDD280 pKa = 3.54PRR282 pKa = 11.84RR283 pKa = 11.84EE284 pKa = 3.59ADD286 pKa = 3.03AA287 pKa = 5.07

Molecular weight: 30.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q9DWB3|Q9DWB3_RCMVM PR89 (Fragment) OS=Rat cytomegalovirus (strain Maastricht) OX=79700 GN=R89-EX2 PE=4 SV=1
MM1 pKa = 7.52TFMLAPPMLLLLVCAARR18 pKa = 11.84GRR20 pKa = 11.84GPGGPSPGGAGFALNVTVVKK40 pKa = 11.03GEE42 pKa = 4.25AGPGARR48 pKa = 11.84PAGDD52 pKa = 3.27PTAEE56 pKa = 3.94KK57 pKa = 10.26VRR59 pKa = 11.84RR60 pKa = 11.84AFRR63 pKa = 11.84PVLSLWFRR71 pKa = 11.84AEE73 pKa = 3.93SGRR76 pKa = 11.84KK77 pKa = 7.71FDD79 pKa = 4.09RR80 pKa = 11.84VRR82 pKa = 11.84FLLTCNGTHH91 pKa = 6.55GAVHH95 pKa = 6.89MYY97 pKa = 10.47SPPTTRR103 pKa = 11.84ALSASARR110 pKa = 11.84NGSDD114 pKa = 3.04QLVVQAFSRR123 pKa = 11.84RR124 pKa = 11.84QCAARR129 pKa = 11.84AIRR132 pKa = 11.84LVVTEE137 pKa = 4.67LYY139 pKa = 10.83GPLRR143 pKa = 11.84ALFRR147 pKa = 11.84EE148 pKa = 4.2ACEE151 pKa = 4.26VYY153 pKa = 10.45GASDD157 pKa = 3.9DD158 pKa = 4.57SEE160 pKa = 4.96SSTVTKK166 pKa = 10.8DD167 pKa = 2.88RR168 pKa = 11.84GSSNDD173 pKa = 3.33PYY175 pKa = 11.15ALSSVFVLFMICLAVSFLVCVWALASEE202 pKa = 4.47LKK204 pKa = 10.53KK205 pKa = 10.63RR206 pKa = 11.84ATEE209 pKa = 3.86EE210 pKa = 3.73RR211 pKa = 11.84DD212 pKa = 3.27LEE214 pKa = 4.4GRR216 pKa = 11.84AGTRR220 pKa = 11.84TGGARR225 pKa = 11.84GTTGPAASRR234 pKa = 11.84VRR236 pKa = 11.84EE237 pKa = 4.18TAAVPPPPPTAAEE250 pKa = 4.3SSTSDD255 pKa = 2.88STARR259 pKa = 11.84LLRR262 pKa = 4.41

Molecular weight: 27.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

167

0

167

65855

34

2100

394.3

43.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.671 ± 0.328

2.089 ± 0.105

5.839 ± 0.158

5.801 ± 0.17

3.787 ± 0.129

7.635 ± 0.302

2.284 ± 0.103

3.833 ± 0.162

2.878 ± 0.136

8.734 ± 0.226

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.833 ± 0.081

3.029 ± 0.153

6.335 ± 0.235

2.373 ± 0.103

9.697 ± 0.297

7.509 ± 0.207

6.167 ± 0.227

7.562 ± 0.204

1.072 ± 0.065

2.873 ± 0.099
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski