Proteus phage vB_PmiP_Pm5460

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Molineuxvirinae; Acadevirus; Proteus virus Pm5460

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0G2SS21|A0A0G2SS21_9CAUD Scaffolding protein OS=Proteus phage vB_PmiP_Pm5460 OX=1636249 GN=Pm5460_34 PE=4 SV=1
MM1 pKa = 7.56FDD3 pKa = 3.64SMKK6 pKa = 10.26ATLAVEE12 pKa = 3.84TAEE15 pKa = 5.26AIFEE19 pKa = 4.12GLSCGIEE26 pKa = 3.87PSPTLLADD34 pKa = 3.58AEE36 pKa = 4.37EE37 pKa = 4.52YY38 pKa = 10.54GINVHH43 pKa = 7.19AIRR46 pKa = 11.84SKK48 pKa = 10.66VEE50 pKa = 3.57EE51 pKa = 4.49LYY53 pKa = 11.21GSGEE57 pKa = 4.14EE58 pKa = 4.21EE59 pKa = 3.26TDD61 pKa = 3.72YY62 pKa = 11.96

Molecular weight: 6.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0G2SS00|A0A0G2SS00_9CAUD Uncharacterized protein OS=Proteus phage vB_PmiP_Pm5460 OX=1636249 GN=Pm5460_14 PE=4 SV=1
MM1 pKa = 7.79RR2 pKa = 11.84NYY4 pKa = 10.54EE5 pKa = 4.18KK6 pKa = 9.56ITRR9 pKa = 11.84KK10 pKa = 9.88SSNRR14 pKa = 11.84YY15 pKa = 9.81DD16 pKa = 3.56MEE18 pKa = 4.48EE19 pKa = 4.23GQMKK23 pKa = 9.79GRR25 pKa = 11.84KK26 pKa = 8.67LNKK29 pKa = 8.27TKK31 pKa = 10.6RR32 pKa = 11.84GTSMKK37 pKa = 10.5RR38 pKa = 11.84MWEE41 pKa = 4.01VV42 pKa = 2.63

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

53

0

53

13785

38

1273

260.1

29.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.864 ± 0.501

1.008 ± 0.165

6.376 ± 0.259

7.269 ± 0.366

3.816 ± 0.129

7.573 ± 0.313

1.937 ± 0.214

5.803 ± 0.224

6.964 ± 0.271

8.009 ± 0.276

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.105 ± 0.177

4.527 ± 0.182

3.25 ± 0.182

4.2 ± 0.243

5.143 ± 0.284

5.999 ± 0.313

5.448 ± 0.306

6.79 ± 0.306

1.32 ± 0.14

3.598 ± 0.304
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski