Leptonychotes weddellii papillomavirus 1
Average proteome isoelectric point is 6.39
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2I8B2P1|A0A2I8B2P1_9PAPI Major capsid protein L1 OS=Leptonychotes weddellii papillomavirus 1 OX=2077302 GN=L1 PE=3 SV=1
MM1 pKa = 7.55 HH2 pKa = 7.4 GEE4 pKa = 3.99 KK5 pKa = 9.71 ATLRR9 pKa = 11.84 DD10 pKa = 3.51 IVLEE14 pKa = 4.12 EE15 pKa = 4.07 QPDD18 pKa = 3.88 AVDD21 pKa = 3.94 LVCYY25 pKa = 9.9 EE26 pKa = 3.82 QMPPQEE32 pKa = 4.34 EE33 pKa = 4.3 EE34 pKa = 4.12 RR35 pKa = 11.84 PGTVTYY41 pKa = 8.91 PYY43 pKa = 10.43 RR44 pKa = 11.84 VATDD48 pKa = 3.33 CGICKK53 pKa = 9.1 RR54 pKa = 11.84 TVRR57 pKa = 11.84 CVCLAEE63 pKa = 4.3 EE64 pKa = 4.1 SDD66 pKa = 3.48 VRR68 pKa = 11.84 IFQEE72 pKa = 4.15 LLCGTFCFVCQSCARR87 pKa = 11.84 KK88 pKa = 8.55 QHH90 pKa = 5.78 FNYY93 pKa = 10.52 GGG95 pKa = 3.11
Molecular weight: 10.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.854
IPC2_protein 4.991
IPC_protein 4.825
Toseland 4.711
ProMoST 4.927
Dawson 4.774
Bjellqvist 4.914
Wikipedia 4.622
Rodwell 4.698
Grimsley 4.635
Solomon 4.774
Lehninger 4.724
Nozaki 4.889
DTASelect 4.991
Thurlkill 4.724
EMBOSS 4.647
Sillero 4.965
Patrickios 0.807
IPC_peptide 4.787
IPC2_peptide 4.965
IPC2.peptide.svr19 4.896
Protein with the highest isoelectric point:
>tr|A0A2I8B2N4|A0A2I8B2N4_9PAPI Protein E7 OS=Leptonychotes weddellii papillomavirus 1 OX=2077302 GN=E7 PE=3 SV=1
MM1 pKa = 7.61 EE2 pKa = 5.38 NLNKK6 pKa = 10.04 ILDD9 pKa = 3.98 SLQEE13 pKa = 4.12 QILTLYY19 pKa = 10.31 EE20 pKa = 4.18 EE21 pKa = 4.62 NSTNLTAHH29 pKa = 5.61 IRR31 pKa = 11.84 LWRR34 pKa = 11.84 LMRR37 pKa = 11.84 RR38 pKa = 11.84 EE39 pKa = 3.68 QVFFYY44 pKa = 9.86 WARR47 pKa = 11.84 QQGVTRR53 pKa = 11.84 VGMRR57 pKa = 11.84 PLPSQASSQTKK68 pKa = 9.23 AKK70 pKa = 10.12 EE71 pKa = 4.07 AIEE74 pKa = 3.84 QEE76 pKa = 4.29 IYY78 pKa = 9.32 VTSLSQSRR86 pKa = 11.84 YY87 pKa = 9.54 GDD89 pKa = 3.78 EE90 pKa = 4.03 PWTIGQTSRR99 pKa = 11.84 EE100 pKa = 3.97 RR101 pKa = 11.84 LLADD105 pKa = 3.45 PQYY108 pKa = 10.84 CFKK111 pKa = 10.92 KK112 pKa = 10.62 DD113 pKa = 3.51 GVQLEE118 pKa = 4.15 VRR120 pKa = 11.84 FDD122 pKa = 3.72 GDD124 pKa = 3.15 KK125 pKa = 11.11 EE126 pKa = 4.05 NLVRR130 pKa = 11.84 YY131 pKa = 6.77 TFWQTIYY138 pKa = 10.55 YY139 pKa = 8.98 QDD141 pKa = 6.0 AMDD144 pKa = 4.29 EE145 pKa = 4.21 WQKK148 pKa = 9.34 TAGLVDD154 pKa = 3.61 SCGLYY159 pKa = 10.5 YY160 pKa = 10.71 VDD162 pKa = 4.37 AQGLKK167 pKa = 10.31 LYY169 pKa = 10.67 YY170 pKa = 10.37 VDD172 pKa = 6.31 FKK174 pKa = 11.83 AEE176 pKa = 3.6 AAKK179 pKa = 10.64 YY180 pKa = 10.15 SRR182 pKa = 11.84 TGLYY186 pKa = 9.49 EE187 pKa = 3.63 VLGNYY192 pKa = 7.1 VTTDD196 pKa = 2.89 VSTSCSGVPHH206 pKa = 6.78 TAASDD211 pKa = 3.68 YY212 pKa = 11.31 TDD214 pKa = 5.02 AEE216 pKa = 4.33 DD217 pKa = 3.92 TAQQRR222 pKa = 11.84 PPARR226 pKa = 11.84 HH227 pKa = 6.83 AITTPKK233 pKa = 8.56 KK234 pKa = 9.02 TSAAPPRR241 pKa = 11.84 RR242 pKa = 11.84 YY243 pKa = 9.08 RR244 pKa = 11.84 VRR246 pKa = 11.84 RR247 pKa = 11.84 SRR249 pKa = 11.84 SRR251 pKa = 11.84 SPRR254 pKa = 11.84 FSSTPTRR261 pKa = 11.84 PRR263 pKa = 11.84 GGVRR267 pKa = 11.84 GPRR270 pKa = 11.84 QRR272 pKa = 11.84 QRR274 pKa = 11.84 EE275 pKa = 4.05 PRR277 pKa = 11.84 STSRR281 pKa = 11.84 SAEE284 pKa = 3.71 RR285 pKa = 11.84 VAPPSAEE292 pKa = 3.9 EE293 pKa = 3.64 VGRR296 pKa = 11.84 RR297 pKa = 11.84 HH298 pKa = 4.79 QTPSKK303 pKa = 10.22 RR304 pKa = 11.84 SGGRR308 pKa = 11.84 IARR311 pKa = 11.84 LIADD315 pKa = 3.66 ARR317 pKa = 11.84 DD318 pKa = 3.48 PPVIIVKK325 pKa = 10.62 GDD327 pKa = 3.47 GNRR330 pKa = 11.84 VKK332 pKa = 10.81 CLRR335 pKa = 11.84 FRR337 pKa = 11.84 LKK339 pKa = 10.89 SKK341 pKa = 10.61 FPTFFDD347 pKa = 4.78 KK348 pKa = 10.82 ISTTWTWTSSDD359 pKa = 3.11 TTDD362 pKa = 2.92 RR363 pKa = 11.84 VGSSRR368 pKa = 11.84 MLISFTDD375 pKa = 3.36 HH376 pKa = 5.14 QQRR379 pKa = 11.84 QVFLDD384 pKa = 3.87 SVPLPSSVRR393 pKa = 11.84 FALGSFDD400 pKa = 4.26 SLL402 pKa = 4.03
Molecular weight: 45.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.92
IPC2_protein 9.282
IPC_protein 9.516
Toseland 9.809
ProMoST 9.648
Dawson 10.101
Bjellqvist 9.838
Wikipedia 10.306
Rodwell 10.277
Grimsley 10.189
Solomon 10.131
Lehninger 10.072
Nozaki 9.853
DTASelect 9.823
Thurlkill 9.926
EMBOSS 10.248
Sillero 10.014
Patrickios 5.537
IPC_peptide 10.116
IPC2_peptide 8.653
IPC2.peptide.svr19 8.271
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
2356
95
600
392.7
44.38
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.9 ± 0.392
2.037 ± 0.672
5.9 ± 0.175
6.409 ± 0.404
5.008 ± 0.303
6.282 ± 0.72
1.995 ± 0.156
4.839 ± 0.478
5.178 ± 0.773
9.041 ± 0.65
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.528 ± 0.265
4.202 ± 0.922
6.494 ± 1.232
4.287 ± 0.541
6.706 ± 1.069
7.81 ± 0.815
5.56 ± 0.736
6.07 ± 0.452
1.316 ± 0.426
3.438 ± 0.254
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here