Streptococcus satellite phage Javan317
Average proteome isoelectric point is 7.19
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 21 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZK14|A0A4D5ZK14_9VIRU Integrase/recombinase OS=Streptococcus satellite phage Javan317 OX=2558632 GN=JavanS317_0021 PE=3 SV=1
MM1 pKa = 7.77 NEE3 pKa = 4.29 LDD5 pKa = 4.63 LSNTQALIFTVILIGFLMYY24 pKa = 10.79 LNHH27 pKa = 7.5 LDD29 pKa = 3.65 SQKK32 pKa = 10.27 SARR35 pKa = 11.84 IEE37 pKa = 4.02 RR38 pKa = 11.84 EE39 pKa = 3.78 STQTIEE45 pKa = 4.28 TTSEE49 pKa = 4.08 EE50 pKa = 4.12 LSPDD54 pKa = 3.18 YY55 pKa = 11.12 GRR57 pKa = 11.84 YY58 pKa = 8.62 IQLAGVKK65 pKa = 8.9 PWGYY69 pKa = 11.3
Molecular weight: 7.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.637
IPC2_protein 4.787
IPC_protein 4.546
Toseland 4.418
ProMoST 4.647
Dawson 4.482
Bjellqvist 4.635
Wikipedia 4.355
Rodwell 4.393
Grimsley 4.329
Solomon 4.482
Lehninger 4.431
Nozaki 4.609
DTASelect 4.698
Thurlkill 4.418
EMBOSS 4.368
Sillero 4.66
Patrickios 3.592
IPC_peptide 4.482
IPC2_peptide 4.66
IPC2.peptide.svr19 4.595
Protein with the highest isoelectric point:
>tr|A0A4D5ZJJ5|A0A4D5ZJJ5_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan317 OX=2558632 GN=JavanS317_0013 PE=4 SV=1
MM1 pKa = 8.07 RR2 pKa = 11.84 YY3 pKa = 10.02 LEE5 pKa = 5.8 AGATWEE11 pKa = 4.69 DD12 pKa = 3.61 VAEE15 pKa = 4.52 KK16 pKa = 10.76 LNKK19 pKa = 5.44 TTRR22 pKa = 11.84 YY23 pKa = 8.12 INSRR27 pKa = 11.84 RR28 pKa = 11.84 KK29 pKa = 9.58 VIAKK33 pKa = 9.92 RR34 pKa = 11.84 FVEE37 pKa = 4.28 LKK39 pKa = 10.67 GYY41 pKa = 10.26
Molecular weight: 4.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.167
IPC2_protein 9.487
IPC_protein 9.663
Toseland 10.145
ProMoST 9.867
Dawson 10.335
Bjellqvist 9.984
Wikipedia 10.496
Rodwell 10.76
Grimsley 10.394
Solomon 10.379
Lehninger 10.35
Nozaki 10.101
DTASelect 9.984
Thurlkill 10.175
EMBOSS 10.54
Sillero 10.233
Patrickios 10.54
IPC_peptide 10.379
IPC2_peptide 8.565
IPC2.peptide.svr19 8.555
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
21
0
21
3326
41
388
158.4
18.46
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.42 ± 0.502
0.722 ± 0.108
6.254 ± 0.422
7.547 ± 0.727
5.292 ± 0.673
4.149 ± 0.457
1.774 ± 0.257
7.877 ± 0.565
11.004 ± 0.552
9.741 ± 0.357
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.135 ± 0.228
6.404 ± 0.467
2.285 ± 0.344
4.029 ± 0.273
4.48 ± 0.326
6.164 ± 0.342
5.683 ± 0.634
4.961 ± 0.436
0.692 ± 0.117
4.39 ± 0.387
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here