Gordonia phage Tanis
Average proteome isoelectric point is 6.06
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 93 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5P8D9C0|A0A5P8D9C0_9CAUD Uncharacterized protein OS=Gordonia phage Tanis OX=2652415 GN=33 PE=4 SV=1
MM1 pKa = 7.8 SEE3 pKa = 4.86 LKK5 pKa = 9.67 WDD7 pKa = 3.72 SAGEE11 pKa = 3.92 RR12 pKa = 11.84 LYY14 pKa = 10.32 EE15 pKa = 4.01 TGVDD19 pKa = 3.47 HH20 pKa = 6.95 GVLYY24 pKa = 10.55 IPDD27 pKa = 3.57 NMGEE31 pKa = 4.09 YY32 pKa = 10.64 SNGYY36 pKa = 8.4 AWNGLTSVSEE46 pKa = 4.39 SPSGAEE52 pKa = 4.81 SNPQYY57 pKa = 11.32 ADD59 pKa = 3.31 NIKK62 pKa = 10.1 YY63 pKa = 10.68 LNLISAEE70 pKa = 4.05 EE71 pKa = 3.95 FGATIEE77 pKa = 4.25 AFTYY81 pKa = 10.1 PDD83 pKa = 4.39 EE84 pKa = 5.31 FSQCDD89 pKa = 3.55 GTALIGGVQIAQQTRR104 pKa = 11.84 KK105 pKa = 10.29 SFGFSYY111 pKa = 9.38 RR112 pKa = 11.84 TLIGNDD118 pKa = 3.28 LVGTDD123 pKa = 4.08 FGYY126 pKa = 10.58 KK127 pKa = 8.59 IHH129 pKa = 7.01 LVYY132 pKa = 10.66 GCQAAPSEE140 pKa = 4.53 KK141 pKa = 10.27 SRR143 pKa = 11.84 STVNDD148 pKa = 3.36 SPEE151 pKa = 3.65 AATFSWEE158 pKa = 4.0 LTTNPVPVAGINADD172 pKa = 3.16 TGKK175 pKa = 9.83 PYY177 pKa = 10.75 RR178 pKa = 11.84 PTAHH182 pKa = 5.59 VTVDD186 pKa = 3.47 STKK189 pKa = 10.35 VSASALAALEE199 pKa = 4.44 EE200 pKa = 4.31 ILYY203 pKa = 8.44 GTASEE208 pKa = 4.65 DD209 pKa = 3.45 PRR211 pKa = 11.84 MPAPEE216 pKa = 4.16 EE217 pKa = 3.85 VLEE220 pKa = 4.17 LVGDD224 pKa = 4.34 ALTEE228 pKa = 4.14 VTPMAATFDD237 pKa = 4.13 APSDD241 pKa = 3.82 TVTIPSQAGVVYY253 pKa = 10.48 KK254 pKa = 10.54 IGGVPVVAGDD264 pKa = 3.54 RR265 pKa = 11.84 VITVEE270 pKa = 4.13 TTVMAEE276 pKa = 3.72 AAIGYY281 pKa = 7.18 SFPDD285 pKa = 3.68 GVTTSWTFDD294 pKa = 3.54 PAA296 pKa = 3.86
Molecular weight: 31.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.862
IPC2_protein 4.113
IPC_protein 4.075
Toseland 3.884
ProMoST 4.202
Dawson 4.037
Bjellqvist 4.19
Wikipedia 3.923
Rodwell 3.91
Grimsley 3.795
Solomon 4.024
Lehninger 3.986
Nozaki 4.139
DTASelect 4.317
Thurlkill 3.91
EMBOSS 3.935
Sillero 4.19
Patrickios 1.926
IPC_peptide 4.037
IPC2_peptide 4.177
IPC2.peptide.svr19 4.078
Protein with the highest isoelectric point:
>tr|A0A5P8DC03|A0A5P8DC03_9CAUD Uncharacterized protein OS=Gordonia phage Tanis OX=2652415 GN=86 PE=4 SV=1
MM1 pKa = 7.69 ASNKK5 pKa = 9.61 RR6 pKa = 11.84 KK7 pKa = 9.84 YY8 pKa = 10.88 GLFNFIVDD16 pKa = 3.62 ITLSLLTGGLWLIWIFCRR34 pKa = 11.84 EE35 pKa = 3.77 MRR37 pKa = 11.84 RR38 pKa = 3.48
Molecular weight: 4.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.323
IPC2_protein 9.545
IPC_protein 10.131
Toseland 10.467
ProMoST 10.833
Dawson 10.584
Bjellqvist 10.292
Wikipedia 10.774
Rodwell 10.877
Grimsley 10.643
Solomon 10.701
Lehninger 10.672
Nozaki 10.482
DTASelect 10.262
Thurlkill 10.482
EMBOSS 10.862
Sillero 10.526
Patrickios 10.804
IPC_peptide 10.701
IPC2_peptide 9.399
IPC2.peptide.svr19 8.671
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
93
0
93
18493
30
1704
198.8
22.06
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.598 ± 0.494
0.6 ± 0.088
6.289 ± 0.264
7.046 ± 0.395
3.553 ± 0.237
7.3 ± 0.43
1.903 ± 0.21
5.927 ± 0.275
5.889 ± 0.318
8.095 ± 0.352
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.828 ± 0.17
4.472 ± 0.162
3.974 ± 0.233
3.45 ± 0.166
4.948 ± 0.302
6.7 ± 0.23
6.3 ± 0.253
7.408 ± 0.238
1.314 ± 0.096
3.407 ± 0.284
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here