Methylobacterium sp. BTF04

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Methylobacteriaceae; Methylobacterium; unclassified Methylobacterium

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4688 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M0HUF4|A0A6M0HUF4_9RHIZ Peptide ABC transporter substrate-binding protein OS=Methylobacterium sp. BTF04 OX=2708300 GN=G3T14_18815 PE=3 SV=1
MM1 pKa = 7.85IDD3 pKa = 3.18LTGYY7 pKa = 11.11KK8 pKa = 9.17LTFDD12 pKa = 4.94DD13 pKa = 4.68EE14 pKa = 4.8FNALSVSATGAGTVWGDD31 pKa = 3.45TRR33 pKa = 11.84PGSILRR39 pKa = 11.84PGVDD43 pKa = 2.43IGFGQSAFVDD53 pKa = 4.11PSIGVQPFAVVDD65 pKa = 3.75GAVQIKK71 pKa = 9.56AAPATGATADD81 pKa = 5.03LINPGLWTSGLLQSANSFSQTYY103 pKa = 10.26GYY105 pKa = 11.19FEE107 pKa = 4.25MRR109 pKa = 11.84ADD111 pKa = 3.9LPGTAGTWPGFWLLAANGVWPPEE134 pKa = 3.92LDD136 pKa = 3.09IVEE139 pKa = 4.92AFGADD144 pKa = 3.31PTLTNTVHH152 pKa = 6.54TAQTGTNTYY161 pKa = 7.34QTVYY165 pKa = 8.55THH167 pKa = 6.67EE168 pKa = 4.93PNLLSGFHH176 pKa = 6.15TFGALWTPTTITFTFDD192 pKa = 2.84GFAVGQIATPADD204 pKa = 3.81MHH206 pKa = 6.19SAMYY210 pKa = 9.53PVLALALQRR219 pKa = 11.84DD220 pKa = 4.14VAGITNDD227 pKa = 3.55PKK229 pKa = 10.95TMQVDD234 pKa = 3.55YY235 pKa = 11.32VRR237 pKa = 11.84AYY239 pKa = 10.88SLDD242 pKa = 3.36ASAVEE247 pKa = 4.41VALQTVSSPDD257 pKa = 3.37GVNTANLYY265 pKa = 10.53GAVATNSAPAPVPVAPGADD284 pKa = 3.06TLTIHH289 pKa = 6.87VSGDD293 pKa = 3.04SWNGAPTFILSVDD306 pKa = 3.71GQRR309 pKa = 11.84VGGLFDD315 pKa = 3.48VTALHH320 pKa = 6.84AQGQTQDD327 pKa = 3.65VTVHH331 pKa = 6.46GDD333 pKa = 3.62FGTGPHH339 pKa = 5.47QVSITYY345 pKa = 10.44INDD348 pKa = 3.29ANSNPSYY355 pKa = 10.78DD356 pKa = 3.44AAHH359 pKa = 5.81NVVLNDD365 pKa = 3.2RR366 pKa = 11.84NLFVSGLEE374 pKa = 4.27LDD376 pKa = 3.77GTHH379 pKa = 6.55YY380 pKa = 9.64GTGAVVSNTASVGWDD395 pKa = 3.06KK396 pKa = 11.4LDD398 pKa = 4.02PNAAVMAANGTVVFQTQAAAGPVVDD423 pKa = 4.91PTPPVPTPTPTPTPTPTPTPTPPVTPTTPVAAGSDD458 pKa = 3.46TLTIHH463 pKa = 7.39ISGDD467 pKa = 3.12SWNGAPAFTLAVDD480 pKa = 3.76GHH482 pKa = 5.26QVGGPFDD489 pKa = 3.81VTALHH494 pKa = 6.75AQGQSQDD501 pKa = 3.25ITVHH505 pKa = 6.31GDD507 pKa = 3.32FGTGSHH513 pKa = 5.44QVAITYY519 pKa = 10.8GNDD522 pKa = 3.11ANSNPTYY529 pKa = 10.53DD530 pKa = 3.33AVHH533 pKa = 5.86NVVLNDD539 pKa = 3.34RR540 pKa = 11.84NLFVSGIDD548 pKa = 4.28LDD550 pKa = 4.1GTHH553 pKa = 6.68YY554 pKa = 9.71GTDD557 pKa = 3.07AVFSNTASAGWDD569 pKa = 3.01RR570 pKa = 11.84LDD572 pKa = 3.81FNAAVMAANGTVVFQTQSAGAPVVDD597 pKa = 4.6PTPTVPTPTPTPPSTPTTPVASGSDD622 pKa = 3.12TLTIHH627 pKa = 7.39ISGDD631 pKa = 3.12SWNGAPAFTLAVDD644 pKa = 3.76GHH646 pKa = 5.26QVGGPFDD653 pKa = 3.81VTALHH658 pKa = 6.75AQGQSQDD665 pKa = 3.25ITVHH669 pKa = 6.36GDD671 pKa = 3.47FGTGPHH677 pKa = 5.34QVAIAYY683 pKa = 9.64FNDD686 pKa = 3.5ANSNPSYY693 pKa = 10.81DD694 pKa = 3.3AANNVVLNDD703 pKa = 3.39RR704 pKa = 11.84NLFVSGIDD712 pKa = 3.96LDD714 pKa = 4.2GSHH717 pKa = 6.93YY718 pKa = 9.03GTSAVVSNTASIGWDD733 pKa = 3.05KK734 pKa = 11.18LDD736 pKa = 3.32PHH738 pKa = 6.87AAVMAADD745 pKa = 5.62GILVFQTSGLVV756 pKa = 3.1

Molecular weight: 78.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M0HQH2|A0A6M0HQH2_9RHIZ Porin family protein OS=Methylobacterium sp. BTF04 OX=2708300 GN=G3T14_07645 PE=4 SV=1
MM1 pKa = 7.19HH2 pKa = 7.99RR3 pKa = 11.84IPATALSARR12 pKa = 11.84LLAAAVLSAGLFVFLPSAPARR33 pKa = 11.84AQSLTAPAGQARR45 pKa = 11.84VTAPGGLRR53 pKa = 11.84GRR55 pKa = 11.84AAMRR59 pKa = 11.84HH60 pKa = 4.63HH61 pKa = 6.72RR62 pKa = 11.84QRR64 pKa = 11.84HH65 pKa = 3.8RR66 pKa = 11.84MMHH69 pKa = 5.73HH70 pKa = 6.01RR71 pKa = 11.84RR72 pKa = 11.84HH73 pKa = 5.79RR74 pKa = 3.57

Molecular weight: 8.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4688

0

4688

1430968

25

2510

305.2

32.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.681 ± 0.057

0.822 ± 0.012

5.774 ± 0.029

5.263 ± 0.033

3.442 ± 0.021

8.831 ± 0.032

2.009 ± 0.018

4.826 ± 0.025

2.797 ± 0.032

10.242 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.16 ± 0.017

2.234 ± 0.02

5.501 ± 0.032

2.822 ± 0.021

7.806 ± 0.037

5.188 ± 0.025

5.684 ± 0.028

7.623 ± 0.029

1.23 ± 0.013

2.064 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski