Amino acid dipepetide frequency for Methylobacterium sp. BTF04

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
19.671AlaAla: 19.671 ± 0.194
1.233AlaCys: 1.233 ± 0.033
7.469AlaAsp: 7.469 ± 0.084
7.926AlaGlu: 7.926 ± 0.102
4.674AlaPhe: 4.674 ± 0.064
11.638AlaGly: 11.638 ± 0.114
2.512AlaHis: 2.512 ± 0.047
6.63AlaIle: 6.63 ± 0.076
4.037AlaLys: 4.037 ± 0.073
15.189AlaLeu: 15.189 ± 0.117
3.572AlaMet: 3.572 ± 0.052
2.754AlaAsn: 2.754 ± 0.05
6.523AlaPro: 6.523 ± 0.093
4.222AlaGln: 4.222 ± 0.06
10.431AlaArg: 10.431 ± 0.095
6.835AlaSer: 6.835 ± 0.073
7.188AlaThr: 7.188 ± 0.085
9.847AlaVal: 9.847 ± 0.083
1.685AlaTrp: 1.685 ± 0.04
2.779AlaTyr: 2.779 ± 0.042
0.0AlaXaa: 0.0 ± 0.0
Cys
1.035CysAla: 1.035 ± 0.032
0.103CysCys: 0.103 ± 0.008
0.538CysAsp: 0.538 ± 0.022
0.413CysGlu: 0.413 ± 0.017
0.271CysPhe: 0.271 ± 0.017
0.904CysGly: 0.904 ± 0.028
0.2CysHis: 0.2 ± 0.013
0.365CysIle: 0.365 ± 0.018
0.171CysLys: 0.171 ± 0.012
0.917CysLeu: 0.917 ± 0.025
0.145CysMet: 0.145 ± 0.011
0.186CysAsn: 0.186 ± 0.011
0.437CysPro: 0.437 ± 0.018
0.19CysGln: 0.19 ± 0.012
0.662CysArg: 0.662 ± 0.025
0.38CysSer: 0.38 ± 0.015
0.414CysThr: 0.414 ± 0.018
0.646CysVal: 0.646 ± 0.026
0.101CysTrp: 0.101 ± 0.009
0.147CysTyr: 0.147 ± 0.01
0.0CysXaa: 0.0 ± 0.0
Asp
8.017AspAla: 8.017 ± 0.088
0.419AspCys: 0.419 ± 0.017
3.06AspAsp: 3.06 ± 0.054
3.164AspGlu: 3.164 ± 0.051
1.913AspPhe: 1.913 ± 0.036
5.75AspGly: 5.75 ± 0.074
1.241AspHis: 1.241 ± 0.031
2.565AspIle: 2.565 ± 0.045
1.436AspLys: 1.436 ± 0.039
6.721AspLeu: 6.721 ± 0.068
1.006AspMet: 1.006 ± 0.027
1.034AspAsn: 1.034 ± 0.03
3.907AspPro: 3.907 ± 0.059
1.473AspGln: 1.473 ± 0.033
4.803AspArg: 4.803 ± 0.064
2.088AspSer: 2.088 ± 0.038
2.823AspThr: 2.823 ± 0.05
4.216AspVal: 4.216 ± 0.058
0.869AspTrp: 0.869 ± 0.025
1.233AspTyr: 1.233 ± 0.028
0.0AspXaa: 0.0 ± 0.0
Glu
8.447GluAla: 8.447 ± 0.094
0.319GluCys: 0.319 ± 0.014
2.468GluAsp: 2.468 ± 0.045
2.438GluGlu: 2.438 ± 0.047
1.46GluPhe: 1.46 ± 0.033
4.171GluGly: 4.171 ± 0.058
1.065GluHis: 1.065 ± 0.028
3.224GluIle: 3.224 ± 0.051
1.605GluLys: 1.605 ± 0.037
4.134GluLeu: 4.134 ± 0.057
1.24GluMet: 1.24 ± 0.033
1.243GluAsn: 1.243 ± 0.029
2.887GluPro: 2.887 ± 0.051
1.512GluGln: 1.512 ± 0.033
5.319GluArg: 5.319 ± 0.075
2.378GluSer: 2.378 ± 0.04
3.752GluThr: 3.752 ± 0.048
3.579GluVal: 3.579 ± 0.049
0.558GluTrp: 0.558 ± 0.021
0.828GluTyr: 0.828 ± 0.025
0.0GluXaa: 0.0 ± 0.0
Phe
4.576PheAla: 4.576 ± 0.062
0.34PheCys: 0.34 ± 0.014
2.433PheAsp: 2.433 ± 0.045
1.942PheGlu: 1.942 ± 0.033
1.199PhePhe: 1.199 ± 0.033
3.572PheGly: 3.572 ± 0.055
0.679PheHis: 0.679 ± 0.024
1.303PheIle: 1.303 ± 0.036
0.921PheLys: 0.921 ± 0.025
3.243PheLeu: 3.243 ± 0.055
0.604PheMet: 0.604 ± 0.02
0.822PheAsn: 0.822 ± 0.022
1.504PhePro: 1.504 ± 0.037
0.881PheGln: 0.881 ± 0.026
2.301PheArg: 2.301 ± 0.049
2.022PheSer: 2.022 ± 0.04
1.998PheThr: 1.998 ± 0.038
2.809PheVal: 2.809 ± 0.049
0.469PheTrp: 0.469 ± 0.02
0.799PheTyr: 0.799 ± 0.027
0.0PheXaa: 0.0 ± 0.0
Gly
10.483GlyAla: 10.483 ± 0.094
0.841GlyCys: 0.841 ± 0.027
4.517GlyAsp: 4.517 ± 0.058
4.529GlyGlu: 4.529 ± 0.066
3.672GlyPhe: 3.672 ± 0.052
7.414GlyGly: 7.414 ± 0.112
1.992GlyHis: 1.992 ± 0.038
4.757GlyIle: 4.757 ± 0.063
2.627GlyLys: 2.627 ± 0.052
10.062GlyLeu: 10.062 ± 0.111
1.973GlyMet: 1.973 ± 0.036
1.847GlyAsn: 1.847 ± 0.039
4.162GlyPro: 4.162 ± 0.054
2.76GlyGln: 2.76 ± 0.046
7.266GlyArg: 7.266 ± 0.078
4.676GlySer: 4.676 ± 0.067
5.206GlyThr: 5.206 ± 0.07
5.936GlyVal: 5.936 ± 0.067
1.308GlyTrp: 1.308 ± 0.031
2.284GlyTyr: 2.284 ± 0.044
0.0GlyXaa: 0.0 ± 0.0
His
2.615HisAla: 2.615 ± 0.047
0.2HisCys: 0.2 ± 0.013
1.309HisAsp: 1.309 ± 0.034
0.927HisGlu: 0.927 ± 0.027
0.744HisPhe: 0.744 ± 0.022
1.962HisGly: 1.962 ± 0.04
0.627HisHis: 0.627 ± 0.025
0.786HisIle: 0.786 ± 0.026
0.392HisLys: 0.392 ± 0.017
2.245HisLeu: 2.245 ± 0.042
0.373HisMet: 0.373 ± 0.016
0.405HisAsn: 0.405 ± 0.02
1.364HisPro: 1.364 ± 0.035
0.528HisGln: 0.528 ± 0.022
1.656HisArg: 1.656 ± 0.034
0.785HisSer: 0.785 ± 0.025
0.853HisThr: 0.853 ± 0.024
1.495HisVal: 1.495 ± 0.033
0.317HisTrp: 0.317 ± 0.015
0.512HisTyr: 0.512 ± 0.022
0.0HisXaa: 0.0 ± 0.0
Ile
7.428IleAla: 7.428 ± 0.072
0.435IleCys: 0.435 ± 0.017
3.333IleAsp: 3.333 ± 0.04
3.254IleGlu: 3.254 ± 0.054
1.319IlePhe: 1.319 ± 0.038
4.981IleGly: 4.981 ± 0.067
0.854IleHis: 0.854 ± 0.026
1.738IleIle: 1.738 ± 0.037
1.212IleLys: 1.212 ± 0.031
4.582IleLeu: 4.582 ± 0.061
0.705IleMet: 0.705 ± 0.026
1.092IleAsn: 1.092 ± 0.03
2.312IlePro: 2.312 ± 0.041
1.186IleGln: 1.186 ± 0.03
3.334IleArg: 3.334 ± 0.047
2.231IleSer: 2.231 ± 0.048
2.552IleThr: 2.552 ± 0.043
4.333IleVal: 4.333 ± 0.06
0.485IleTrp: 0.485 ± 0.017
0.895IleTyr: 0.895 ± 0.027
0.0IleXaa: 0.0 ± 0.0
Lys
4.166LysAla: 4.166 ± 0.064
0.131LysCys: 0.131 ± 0.01
1.495LysAsp: 1.495 ± 0.037
1.153LysGlu: 1.153 ± 0.033
0.725LysPhe: 0.725 ± 0.027
2.344LysGly: 2.344 ± 0.044
0.477LysHis: 0.477 ± 0.022
1.371LysIle: 1.371 ± 0.032
0.955LysLys: 0.955 ± 0.034
2.651LysLeu: 2.651 ± 0.05
0.573LysMet: 0.573 ± 0.019
0.711LysAsn: 0.711 ± 0.024
2.052LysPro: 2.052 ± 0.045
0.78LysGln: 0.78 ± 0.025
2.235LysArg: 2.235 ± 0.041
1.573LysSer: 1.573 ± 0.035
1.746LysThr: 1.746 ± 0.036
2.106LysVal: 2.106 ± 0.047
0.247LysTrp: 0.247 ± 0.011
0.474LysTyr: 0.474 ± 0.021
0.0LysXaa: 0.0 ± 0.0
Leu
15.088LeuAla: 15.088 ± 0.127
0.98LeuCys: 0.98 ± 0.026
6.635LeuAsp: 6.635 ± 0.077
4.468LeuGlu: 4.468 ± 0.055
3.404LeuPhe: 3.404 ± 0.059
8.902LeuGly: 8.902 ± 0.091
1.962LeuHis: 1.962 ± 0.042
4.789LeuIle: 4.789 ± 0.073
3.134LeuLys: 3.134 ± 0.048
8.964LeuLeu: 8.964 ± 0.104
2.149LeuMet: 2.149 ± 0.039
2.329LeuAsn: 2.329 ± 0.052
5.667LeuPro: 5.667 ± 0.063
2.526LeuGln: 2.526 ± 0.043
7.633LeuArg: 7.633 ± 0.076
6.301LeuSer: 6.301 ± 0.072
6.013LeuThr: 6.013 ± 0.08
8.325LeuVal: 8.325 ± 0.081
1.099LeuTrp: 1.099 ± 0.032
2.056LeuTyr: 2.056 ± 0.039
0.0LeuXaa: 0.0 ± 0.0
Met
2.812MetAla: 2.812 ± 0.042
0.126MetCys: 0.126 ± 0.01
0.964MetAsp: 0.964 ± 0.029
0.93MetGlu: 0.93 ± 0.026
0.533MetPhe: 0.533 ± 0.017
1.555MetGly: 1.555 ± 0.036
0.396MetHis: 0.396 ± 0.017
1.15MetIle: 1.15 ± 0.028
0.745MetLys: 0.745 ± 0.026
2.111MetLeu: 2.111 ± 0.038
0.57MetMet: 0.57 ± 0.022
0.628MetAsn: 0.628 ± 0.021
1.508MetPro: 1.508 ± 0.035
0.707MetGln: 0.707 ± 0.023
1.914MetArg: 1.914 ± 0.038
1.458MetSer: 1.458 ± 0.031
1.651MetThr: 1.651 ± 0.03
1.47MetVal: 1.47 ± 0.035
0.151MetTrp: 0.151 ± 0.01
0.222MetTyr: 0.222 ± 0.013
0.0MetXaa: 0.0 ± 0.0
Asn
2.989AsnAla: 2.989 ± 0.049
0.18AsnCys: 0.18 ± 0.014
1.192AsnAsp: 1.192 ± 0.032
0.995AsnGlu: 0.995 ± 0.027
0.769AsnPhe: 0.769 ± 0.025
2.152AsnGly: 2.152 ± 0.04
0.446AsnHis: 0.446 ± 0.016
0.989AsnIle: 0.989 ± 0.029
0.552AsnLys: 0.552 ± 0.022
2.382AsnLeu: 2.382 ± 0.05
0.43AsnMet: 0.43 ± 0.018
0.53AsnAsn: 0.53 ± 0.022
1.692AsnPro: 1.692 ± 0.036
0.605AsnGln: 0.605 ± 0.022
1.734AsnArg: 1.734 ± 0.037
0.973AsnSer: 0.973 ± 0.032
1.171AsnThr: 1.171 ± 0.034
1.727AsnVal: 1.727 ± 0.04
0.325AsnTrp: 0.325 ± 0.016
0.508AsnTyr: 0.508 ± 0.021
0.0AsnXaa: 0.0 ± 0.0
Pro
7.261ProAla: 7.261 ± 0.093
0.357ProCys: 0.357 ± 0.018
4.032ProAsp: 4.032 ± 0.061
3.62ProGlu: 3.62 ± 0.055
1.984ProPhe: 1.984 ± 0.034
5.021ProGly: 5.021 ± 0.076
1.099ProHis: 1.099 ± 0.029
2.406ProIle: 2.406 ± 0.041
1.632ProLys: 1.632 ± 0.038
4.814ProLeu: 4.814 ± 0.06
1.225ProMet: 1.225 ± 0.029
1.298ProAsn: 1.298 ± 0.035
3.006ProPro: 3.006 ± 0.066
1.474ProGln: 1.474 ± 0.039
3.517ProArg: 3.517 ± 0.056
2.962ProSer: 2.962 ± 0.048
2.837ProThr: 2.837 ± 0.048
4.595ProVal: 4.595 ± 0.063
0.732ProTrp: 0.732 ± 0.026
1.139ProTyr: 1.139 ± 0.033
0.0ProXaa: 0.0 ± 0.0
Gln
4.425GlnAla: 4.425 ± 0.068
0.169GlnCys: 0.169 ± 0.012
1.436GlnAsp: 1.436 ± 0.038
1.218GlnGlu: 1.218 ± 0.032
0.878GlnPhe: 0.878 ± 0.026
2.465GlnGly: 2.465 ± 0.039
0.545GlnHis: 0.545 ± 0.021
1.588GlnIle: 1.588 ± 0.033
0.841GlnLys: 0.841 ± 0.026
2.152GlnLeu: 2.152 ± 0.045
0.639GlnMet: 0.639 ± 0.023
0.743GlnAsn: 0.743 ± 0.025
1.575GlnPro: 1.575 ± 0.036
0.832GlnGln: 0.832 ± 0.031
2.25GlnArg: 2.25 ± 0.039
1.651GlnSer: 1.651 ± 0.038
1.811GlnThr: 1.811 ± 0.034
2.193GlnVal: 2.193 ± 0.042
0.302GlnTrp: 0.302 ± 0.015
0.508GlnTyr: 0.508 ± 0.017
0.0GlnXaa: 0.0 ± 0.0
Arg
9.534ArgAla: 9.534 ± 0.107
0.561ArgCys: 0.561 ± 0.019
4.675ArgAsp: 4.675 ± 0.059
4.171ArgGlu: 4.171 ± 0.056
3.073ArgPhe: 3.073 ± 0.052
5.473ArgGly: 5.473 ± 0.073
1.895ArgHis: 1.895 ± 0.038
4.426ArgIle: 4.426 ± 0.053
2.001ArgLys: 2.001 ± 0.041
8.767ArgLeu: 8.767 ± 0.102
1.906ArgMet: 1.906 ± 0.041
1.707ArgAsn: 1.707 ± 0.035
4.294ArgPro: 4.294 ± 0.066
2.385ArgGln: 2.385 ± 0.054
6.808ArgArg: 6.808 ± 0.097
4.129ArgSer: 4.129 ± 0.058
4.235ArgThr: 4.235 ± 0.051
5.329ArgVal: 5.329 ± 0.068
0.965ArgTrp: 0.965 ± 0.026
1.725ArgTyr: 1.725 ± 0.039
0.0ArgXaa: 0.0 ± 0.0
Ser
6.575SerAla: 6.575 ± 0.071
0.371SerCys: 0.371 ± 0.018
3.062SerAsp: 3.062 ± 0.056
2.74SerGlu: 2.74 ± 0.047
1.955SerPhe: 1.955 ± 0.04
5.457SerGly: 5.457 ± 0.062
0.99SerHis: 0.99 ± 0.027
2.359SerIle: 2.359 ± 0.039
1.352SerLys: 1.352 ± 0.036
5.564SerLeu: 5.564 ± 0.077
1.073SerMet: 1.073 ± 0.03
1.198SerAsn: 1.198 ± 0.032
2.863SerPro: 2.863 ± 0.048
1.514SerGln: 1.514 ± 0.035
3.697SerArg: 3.697 ± 0.056
2.618SerSer: 2.618 ± 0.058
2.74SerThr: 2.74 ± 0.048
3.99SerVal: 3.99 ± 0.057
0.605SerTrp: 0.605 ± 0.021
1.156SerTyr: 1.156 ± 0.032
0.0SerXaa: 0.0 ± 0.0
Thr
7.004ThrAla: 7.004 ± 0.077
0.444ThrCys: 0.444 ± 0.019
2.974ThrAsp: 2.974 ± 0.048
2.654ThrGlu: 2.654 ± 0.047
1.96ThrPhe: 1.96 ± 0.035
5.64ThrGly: 5.64 ± 0.07
1.02ThrHis: 1.02 ± 0.029
2.932ThrIle: 2.932 ± 0.049
1.519ThrLys: 1.519 ± 0.034
6.693ThrLeu: 6.693 ± 0.067
1.085ThrMet: 1.085 ± 0.03
1.275ThrAsn: 1.275 ± 0.041
3.421ThrPro: 3.421 ± 0.049
1.595ThrGln: 1.595 ± 0.032
3.894ThrArg: 3.894 ± 0.047
2.718ThrSer: 2.718 ± 0.045
3.198ThrThr: 3.198 ± 0.05
4.803ThrVal: 4.803 ± 0.059
0.726ThrTrp: 0.726 ± 0.022
1.281ThrTyr: 1.281 ± 0.035
0.0ThrXaa: 0.0 ± 0.0
Val
10.689ValAla: 10.689 ± 0.095
0.657ValCys: 0.657 ± 0.023
4.185ValAsp: 4.185 ± 0.052
4.52ValGlu: 4.52 ± 0.06
2.627ValPhe: 2.627 ± 0.041
5.907ValGly: 5.907 ± 0.071
1.394ValHis: 1.394 ± 0.03
3.587ValIle: 3.587 ± 0.066
2.017ValLys: 2.017 ± 0.042
7.584ValLeu: 7.584 ± 0.073
1.688ValMet: 1.688 ± 0.037
1.651ValAsn: 1.651 ± 0.038
4.186ValPro: 4.186 ± 0.067
2.069ValGln: 2.069 ± 0.037
5.626ValArg: 5.626 ± 0.074
4.333ValSer: 4.333 ± 0.059
4.777ValThr: 4.777 ± 0.063
6.512ValVal: 6.512 ± 0.079
0.847ValTrp: 0.847 ± 0.025
1.368ValTyr: 1.368 ± 0.037
0.0ValXaa: 0.0 ± 0.0
Trp
1.227TrpAla: 1.227 ± 0.033
0.134TrpCys: 0.134 ± 0.009
0.577TrpAsp: 0.577 ± 0.023
0.46TrpGlu: 0.46 ± 0.016
0.496TrpPhe: 0.496 ± 0.021
0.825TrpGly: 0.825 ± 0.022
0.32TrpHis: 0.32 ± 0.015
0.602TrpIle: 0.602 ± 0.02
0.354TrpLys: 0.354 ± 0.016
1.499TrpLeu: 1.499 ± 0.034
0.319TrpMet: 0.319 ± 0.015
0.361TrpAsn: 0.361 ± 0.018
0.651TrpPro: 0.651 ± 0.022
0.419TrpGln: 0.419 ± 0.018
1.197TrpArg: 1.197 ± 0.031
0.839TrpSer: 0.839 ± 0.024
0.816TrpThr: 0.816 ± 0.025
0.742TrpVal: 0.742 ± 0.023
0.222TrpTrp: 0.222 ± 0.014
0.242TrpTyr: 0.242 ± 0.013
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.772TyrAla: 2.772 ± 0.044
0.224TyrCys: 0.224 ± 0.013
1.386TyrAsp: 1.386 ± 0.032
1.104TyrGlu: 1.104 ± 0.027
0.762TyrPhe: 0.762 ± 0.028
2.182TyrGly: 2.182 ± 0.041
0.38TyrHis: 0.38 ± 0.015
0.693TyrIle: 0.693 ± 0.022
0.461TyrLys: 0.461 ± 0.02
2.15TyrLeu: 2.15 ± 0.043
0.327TyrMet: 0.327 ± 0.014
0.525TyrAsn: 0.525 ± 0.02
0.999TyrPro: 0.999 ± 0.025
0.565TyrGln: 0.565 ± 0.018
1.784TyrArg: 1.784 ± 0.036
0.925TyrSer: 0.925 ± 0.027
1.055TyrThr: 1.055 ± 0.028
1.573TyrVal: 1.573 ± 0.039
0.29TyrTrp: 0.29 ± 0.012
0.484TyrTyr: 0.484 ± 0.019
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4688 proteins (1430968 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski