Lachnellula cervina

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Leotiomycetes; Helotiales; Lachnaceae; Lachnellula

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9411 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7D8UTW6|A0A7D8UTW6_9HELO GLC7-interacting protein 3 OS=Lachnellula cervina OX=1316786 GN=GIP3 PE=4 SV=1
MM1 pKa = 7.56PAANTTAKK9 pKa = 10.32GRR11 pKa = 11.84TATEE15 pKa = 3.71QSTATLTPAFSPPFSITSPDD35 pKa = 3.59ISSPWNDD42 pKa = 3.15FCQSGGPRR50 pKa = 11.84DD51 pKa = 4.08TEE53 pKa = 4.14DD54 pKa = 3.49SQIGTDD60 pKa = 3.44WDD62 pKa = 3.94CLHH65 pKa = 6.9FSTSEE70 pKa = 3.85MLEE73 pKa = 4.07GSSKK77 pKa = 9.54NTVPGIGEE85 pKa = 3.98DD86 pKa = 3.91TACAGLDD93 pKa = 3.4DD94 pKa = 5.2SLNPDD99 pKa = 3.57HH100 pKa = 7.23LLLTTLPWSDD110 pKa = 4.37YY111 pKa = 10.58PPQDD115 pKa = 2.98EE116 pKa = 5.11CLTSFSGPTTICEE129 pKa = 4.15QPDD132 pKa = 3.99PNVLARR138 pKa = 11.84VPIVDD143 pKa = 3.91PVANFTANIIMQMLCIGTVPQPLL166 pKa = 3.34

Molecular weight: 17.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7D8YJR0|A0A7D8YJR0_9HELO Ubiquitin-like domain-containing protein OS=Lachnellula cervina OX=1316786 GN=LCER1_G008191 PE=4 SV=1
RR1 pKa = 7.41KK2 pKa = 9.28AQLRR6 pKa = 11.84LLHH9 pKa = 6.57CCTAALHH16 pKa = 5.84AHH18 pKa = 6.14PAKK21 pKa = 10.58DD22 pKa = 3.13IRR24 pKa = 11.84SRR26 pKa = 11.84RR27 pKa = 11.84HH28 pKa = 4.21WRR30 pKa = 11.84IKK32 pKa = 9.28PHH34 pKa = 6.15VDD36 pKa = 3.0YY37 pKa = 10.91GLCTILFQDD46 pKa = 3.28QVGGLEE52 pKa = 3.96VDD54 pKa = 3.77PDD56 pKa = 3.72HH57 pKa = 6.77TGKK60 pKa = 10.57FLPATLARR68 pKa = 11.84GTCVINVADD77 pKa = 4.43LLQRR81 pKa = 11.84LSNNRR86 pKa = 11.84LKK88 pKa = 9.95STRR91 pKa = 11.84HH92 pKa = 5.28RR93 pKa = 11.84VTPPQLIEE101 pKa = 3.98EE102 pKa = 4.64QKK104 pKa = 9.52RR105 pKa = 11.84TLGPEE110 pKa = 3.99DD111 pKa = 4.3YY112 pKa = 11.16LPARR116 pKa = 11.84YY117 pKa = 8.16STAFFVHH124 pKa = 7.02PAPSHH129 pKa = 4.73TVAPILLPGDD139 pKa = 3.79PASEE143 pKa = 4.13SEE145 pKa = 4.26PVNVRR150 pKa = 11.84SGEE153 pKa = 4.08RR154 pKa = 11.84VSQGGITRR162 pKa = 11.84RR163 pKa = 11.84LICRR167 pKa = 11.84TRR169 pKa = 11.84RR170 pKa = 11.84FKK172 pKa = 10.54MRR174 pKa = 11.84GRR176 pKa = 11.84RR177 pKa = 11.84LTARR181 pKa = 11.84PKK183 pKa = 10.19LYY185 pKa = 10.56RR186 pKa = 11.84SVLCNDD192 pKa = 4.43TIDD195 pKa = 3.4VV196 pKa = 3.8

Molecular weight: 22.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9411

0

9411

4670120

25

5789

496.2

54.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.416 ± 0.022

1.128 ± 0.009

5.51 ± 0.015

6.224 ± 0.023

3.845 ± 0.015

7.122 ± 0.02

2.251 ± 0.011

5.227 ± 0.015

5.299 ± 0.024

8.888 ± 0.023

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.192 ± 0.009

3.884 ± 0.013

5.861 ± 0.023

3.838 ± 0.016

5.596 ± 0.021

8.37 ± 0.027

6.035 ± 0.018

6.079 ± 0.015

1.424 ± 0.008

2.811 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski