Burkholderia virus phi6442

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Stanholtvirus

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 71 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A4JX07|A4JX07_9CAUD Gp12 OS=Burkholderia virus phi6442 OX=431891 GN=BPSphi6442_0012 PE=4 SV=1
MM1 pKa = 7.11SVIVVRR7 pKa = 11.84DD8 pKa = 3.52ALQGIGGAKK17 pKa = 9.9GYY19 pKa = 10.65LGVAPEE25 pKa = 4.35KK26 pKa = 10.82APARR30 pKa = 11.84YY31 pKa = 8.38FVVTRR36 pKa = 11.84VHH38 pKa = 6.38GALDD42 pKa = 3.48MALAGPTGGRR52 pKa = 11.84SGSYY56 pKa = 10.36QIDD59 pKa = 3.96CYY61 pKa = 11.59APTFTDD67 pKa = 3.42ADD69 pKa = 4.19RR70 pKa = 11.84LADD73 pKa = 4.0LAVDD77 pKa = 4.0RR78 pKa = 11.84AMSVQDD84 pKa = 3.46RR85 pKa = 11.84FSVGGVDD92 pKa = 5.0EE93 pKa = 5.93LPDD96 pKa = 4.95DD97 pKa = 4.3YY98 pKa = 11.86SADD101 pKa = 3.34TGLFRR106 pKa = 11.84VSLEE110 pKa = 3.95LSVEE114 pKa = 4.27FF115 pKa = 5.22

Molecular weight: 12.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A4JX64|A4JX64_9CAUD Gp69 OS=Burkholderia virus phi6442 OX=431891 GN=BPSphi6442_0070 PE=4 SV=1
MM1 pKa = 7.33SLVSSICVMQSHH13 pKa = 6.24EE14 pKa = 4.14RR15 pKa = 11.84DD16 pKa = 3.55DD17 pKa = 3.79RR18 pKa = 11.84HH19 pKa = 6.8SRR21 pKa = 11.84THH23 pKa = 6.2PVRR26 pKa = 11.84TTAKK30 pKa = 8.96HH31 pKa = 4.96QNSHH35 pKa = 5.86ISSGRR40 pKa = 11.84GNEE43 pKa = 3.88KK44 pKa = 10.34ARR46 pKa = 11.84EE47 pKa = 4.07AFTLRR52 pKa = 11.84ASYY55 pKa = 10.67SIDD58 pKa = 3.13APLPVGEE65 pKa = 4.21VARR68 pKa = 11.84LCGIGHH74 pKa = 7.22LRR76 pKa = 11.84SMIHH80 pKa = 6.6SSRR83 pKa = 11.84LVYY86 pKa = 10.36KK87 pKa = 10.62RR88 pKa = 11.84FFEE91 pKa = 4.47SVRR94 pKa = 11.84II95 pKa = 3.98

Molecular weight: 10.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

71

0

71

14373

40

1354

202.4

22.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.363 ± 0.777

1.28 ± 0.182

5.872 ± 0.197

5.963 ± 0.334

3.124 ± 0.165

8.105 ± 0.346

1.767 ± 0.204

4.919 ± 0.177

4.279 ± 0.222

7.98 ± 0.296

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.421 ± 0.123

3.159 ± 0.217

4.543 ± 0.343

3.966 ± 0.26

7.632 ± 0.361

6.303 ± 0.289

4.968 ± 0.217

7.278 ± 0.329

1.552 ± 0.156

2.526 ± 0.181
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski