Lachnospiraceae bacterium 1_4_56FAA

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; unclassified Lachnospiraceae

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3188 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F7JKD5|F7JKD5_9FIRM Uncharacterized protein OS=Lachnospiraceae bacterium 1_4_56FAA OX=658655 GN=HMPREF0988_00876 PE=4 SV=1
MM1 pKa = 7.27SGYY4 pKa = 10.7YY5 pKa = 10.54QMTLDD10 pKa = 5.74DD11 pKa = 4.23IANDD15 pKa = 3.88TEE17 pKa = 4.4KK18 pKa = 11.18SFGNRR23 pKa = 11.84KK24 pKa = 9.59DD25 pKa = 4.73LLDD28 pKa = 3.74TMSLYY33 pKa = 9.24GTALFLAACIQEE45 pKa = 3.99MDD47 pKa = 3.66VTRR50 pKa = 11.84LTDD53 pKa = 3.14ADD55 pKa = 3.6YY56 pKa = 10.91WLDD59 pKa = 3.7YY60 pKa = 10.94LKK62 pKa = 10.73QEE64 pKa = 4.11VDD66 pKa = 3.72DD67 pKa = 4.86NGVPLEE73 pKa = 4.05

Molecular weight: 8.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F7JI69|F7JI69_9FIRM Uncharacterized protein OS=Lachnospiraceae bacterium 1_4_56FAA OX=658655 GN=HMPREF0988_00023 PE=4 SV=1
RR1 pKa = 8.54RR2 pKa = 11.84EE3 pKa = 3.95EE4 pKa = 3.96PGNRR8 pKa = 11.84QRR10 pKa = 11.84LKK12 pKa = 10.79KK13 pKa = 10.17VVPEE17 pKa = 4.34SGTQRR22 pKa = 11.84GTGNGQRR29 pKa = 11.84SEE31 pKa = 4.23EE32 pKa = 4.27SCTGIRR38 pKa = 11.84YY39 pKa = 9.74AEE41 pKa = 4.21RR42 pKa = 11.84NRR44 pKa = 11.84EE45 pKa = 3.72QAASGRR51 pKa = 11.84KK52 pKa = 9.17LYY54 pKa = 10.44RR55 pKa = 11.84NPVRR59 pKa = 11.84RR60 pKa = 11.84EE61 pKa = 3.62EE62 pKa = 4.15KK63 pKa = 9.52GTGNEE68 pKa = 3.88RR69 pKa = 11.84KK70 pKa = 9.83KK71 pKa = 10.76VVPEE75 pKa = 4.09SGTSGRR81 pKa = 11.84IRR83 pKa = 11.84EE84 pKa = 4.0QEE86 pKa = 3.97AIGRR90 pKa = 11.84KK91 pKa = 9.43LYY93 pKa = 10.27RR94 pKa = 11.84NPVRR98 pKa = 11.84RR99 pKa = 11.84EE100 pKa = 3.72EE101 pKa = 4.52LGTGSDD107 pKa = 3.31RR108 pKa = 11.84KK109 pKa = 10.49KK110 pKa = 10.61VVPEE114 pKa = 4.04SGTQVGTGNGQRR126 pKa = 11.84SEE128 pKa = 4.23EE129 pKa = 4.27SCTGIRR135 pKa = 11.84YY136 pKa = 8.64VRR138 pKa = 3.67

Molecular weight: 15.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3188

0

3188

960594

29

1914

301.3

33.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.539 ± 0.044

1.488 ± 0.019

5.405 ± 0.038

8.295 ± 0.059

4.022 ± 0.035

7.298 ± 0.039

1.801 ± 0.02

7.071 ± 0.039

6.792 ± 0.035

8.88 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.243 ± 0.023

4.036 ± 0.028

3.321 ± 0.024

3.348 ± 0.026

4.696 ± 0.032

5.428 ± 0.029

5.339 ± 0.028

6.905 ± 0.032

1.009 ± 0.016

4.083 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski