Plasmodium falciparum (isolate HB3)

Taxonomy: cellular organisms; Eukaryota; Sar; Alveolata; Apicomplexa; Aconoidasida; Haemosporida; Plasmodiidae; Plasmodium; Plasmodium (Laverania); Plasmodium falciparum

Average proteome isoelectric point is 7.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5452 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0L7K907|A0A0L7K907_PLAFX Uncharacterized protein OS=Plasmodium falciparum (isolate HB3) OX=137071 GN=PFHG_01609 PE=4 SV=1
MM1 pKa = 7.13YY2 pKa = 10.21FIEE5 pKa = 4.9EE6 pKa = 4.1KK7 pKa = 10.16QLYY10 pKa = 9.21YY11 pKa = 10.29PFNEE15 pKa = 3.92TCSRR19 pKa = 11.84YY20 pKa = 9.4RR21 pKa = 11.84RR22 pKa = 11.84IVAEE26 pKa = 4.02EE27 pKa = 3.59QHH29 pKa = 6.61EE30 pKa = 4.31IEE32 pKa = 4.94LDD34 pKa = 3.55EE35 pKa = 4.4EE36 pKa = 4.83SIIVLDD42 pKa = 4.23DD43 pKa = 5.1DD44 pKa = 4.19INIIQEE50 pKa = 4.08EE51 pKa = 4.75YY52 pKa = 11.19EE53 pKa = 4.09NMNSYY58 pKa = 11.21DD59 pKa = 3.87YY60 pKa = 11.86LNLQLYY66 pKa = 10.53NDD68 pKa = 3.85TTMMHH73 pKa = 4.84SHH75 pKa = 6.57EE76 pKa = 4.99VNMQQDD82 pKa = 3.4GDD84 pKa = 3.99INIQQDD90 pKa = 3.17GDD92 pKa = 3.43INMQQDD98 pKa = 3.36GDD100 pKa = 4.26INIQDD105 pKa = 4.33DD106 pKa = 5.15DD107 pKa = 5.05INIQQDD113 pKa = 2.97GDD115 pKa = 3.74INIQQDD121 pKa = 3.07GDD123 pKa = 3.7INIQYY128 pKa = 11.0DD129 pKa = 4.45DD130 pKa = 4.76INIQQDD136 pKa = 2.97GDD138 pKa = 3.83INTEE142 pKa = 3.7QDD144 pKa = 3.25DD145 pKa = 5.38DD146 pKa = 5.12IILLQEE152 pKa = 4.75DD153 pKa = 5.37DD154 pKa = 4.43IDD156 pKa = 4.74LLTDD160 pKa = 3.24EE161 pKa = 6.08DD162 pKa = 4.7INFLEE167 pKa = 4.24QQHH170 pKa = 6.39NDD172 pKa = 3.39LLNEE176 pKa = 3.95EE177 pKa = 4.5SANTNVQNNYY187 pKa = 10.43SFVYY191 pKa = 10.85DD192 pKa = 3.36NDD194 pKa = 3.73YY195 pKa = 11.73NKK197 pKa = 10.36LHH199 pKa = 6.57EE200 pKa = 4.55LAEE203 pKa = 4.4EE204 pKa = 4.36YY205 pKa = 10.81KK206 pKa = 10.3DD207 pKa = 3.57TLNDD211 pKa = 3.41VISRR215 pKa = 11.84MAYY218 pKa = 10.06DD219 pKa = 3.99YY220 pKa = 11.91NDD222 pKa = 3.53LTEE225 pKa = 4.99DD226 pKa = 3.71MNKK229 pKa = 9.46EE230 pKa = 3.38WSFNMWNIRR239 pKa = 11.84WCKK242 pKa = 9.17YY243 pKa = 9.6LEE245 pKa = 3.99NMMEE249 pKa = 4.06EE250 pKa = 3.96VNYY253 pKa = 10.25YY254 pKa = 11.06LNGNFSIDD262 pKa = 3.68DD263 pKa = 3.49KK264 pKa = 11.31KK265 pKa = 11.14QYY267 pKa = 11.14LEE269 pKa = 6.01LLLFWCKK276 pKa = 9.58RR277 pKa = 11.84DD278 pKa = 3.51YY279 pKa = 11.53KK280 pKa = 11.07HH281 pKa = 7.24FIDD284 pKa = 4.4VVKK287 pKa = 10.98KK288 pKa = 9.81EE289 pKa = 3.96WDD291 pKa = 3.37KK292 pKa = 11.63KK293 pKa = 10.97DD294 pKa = 3.66EE295 pKa = 4.49PEE297 pKa = 4.81HH298 pKa = 6.77YY299 pKa = 10.53LEE301 pKa = 4.5RR302 pKa = 5.86

Molecular weight: 36.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0L7KD42|A0A0L7KD42_PLAFX Malaria antigen OS=Plasmodium falciparum (isolate HB3) OX=137071 GN=PFHG_03001 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 10.01KK3 pKa = 10.11DD4 pKa = 3.8RR5 pKa = 11.84EE6 pKa = 4.45PIDD9 pKa = 3.96EE10 pKa = 4.58DD11 pKa = 3.52EE12 pKa = 4.36MRR14 pKa = 11.84ITSTGRR20 pKa = 11.84MTNYY24 pKa = 9.82VNYY27 pKa = 9.53GAKK30 pKa = 9.99ILGDD34 pKa = 3.75EE35 pKa = 4.51DD36 pKa = 3.69KK37 pKa = 11.34KK38 pKa = 11.14SIKK41 pKa = 10.08IKK43 pKa = 9.91ATGNAIGKK51 pKa = 9.51AVTLAEE57 pKa = 4.29IIKK60 pKa = 10.34RR61 pKa = 11.84RR62 pKa = 11.84FKK64 pKa = 10.78GLHH67 pKa = 5.37QITRR71 pKa = 11.84CGSTVITDD79 pKa = 3.53QYY81 pKa = 11.83VSGQDD86 pKa = 3.3NSEE89 pKa = 4.02HH90 pKa = 5.44VVQEE94 pKa = 4.06KK95 pKa = 8.44TVSFIEE101 pKa = 3.91ILLSRR106 pKa = 11.84EE107 pKa = 3.83QLDD110 pKa = 3.78MKK112 pKa = 10.8DD113 pKa = 3.88AGYY116 pKa = 10.2QPPLDD121 pKa = 3.58EE122 pKa = 5.51KK123 pKa = 10.52YY124 pKa = 10.33VKK126 pKa = 10.5EE127 pKa = 4.03MTPEE131 pKa = 4.26EE132 pKa = 4.32IVNSRR137 pKa = 11.84PFRR140 pKa = 11.84RR141 pKa = 11.84GGFRR145 pKa = 11.84PRR147 pKa = 11.84FYY149 pKa = 10.72RR150 pKa = 11.84GFRR153 pKa = 11.84GGRR156 pKa = 11.84GGFLRR161 pKa = 11.84RR162 pKa = 11.84GGYY165 pKa = 9.68RR166 pKa = 11.84GFGDD170 pKa = 3.01RR171 pKa = 11.84VYY173 pKa = 10.69EE174 pKa = 3.93PRR176 pKa = 11.84SSFRR180 pKa = 11.84GGRR183 pKa = 11.84GSGYY187 pKa = 10.08GGNFGRR193 pKa = 11.84GGYY196 pKa = 9.82RR197 pKa = 11.84SGGGMGGGFRR207 pKa = 11.84GGFRR211 pKa = 11.84GGFRR215 pKa = 11.84GGRR218 pKa = 11.84DD219 pKa = 2.89GGYY222 pKa = 9.98RR223 pKa = 11.84GGNRR227 pKa = 11.84GGSRR231 pKa = 11.84SGFRR235 pKa = 11.84GGRR238 pKa = 11.84GGFRR242 pKa = 11.84GGRR245 pKa = 11.84ALSS248 pKa = 3.5

Molecular weight: 27.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5452

0

5452

3784517

21

10299

694.2

81.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

1.965 ± 0.023

1.771 ± 0.015

6.52 ± 0.029

7.149 ± 0.061

4.327 ± 0.027

2.857 ± 0.026

2.422 ± 0.014

9.193 ± 0.037

11.711 ± 0.044

7.546 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.199 ± 0.014

14.403 ± 0.094

1.999 ± 0.02

2.777 ± 0.019

2.655 ± 0.018

6.414 ± 0.025

4.102 ± 0.018

3.807 ± 0.028

0.512 ± 0.009

5.671 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski