Aspergillus turcosus
Average proteome isoelectric point is 6.38
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9174 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A421CTZ5|A0A421CTZ5_9EURO Anaphase-promoting complex subunit 2 OS=Aspergillus turcosus OX=1245748 GN=CFD26_101979 PE=3 SV=1
MM1 pKa = 7.91 PIKK4 pKa = 10.63 LSTILSSLFYY14 pKa = 10.87 LLPLYY19 pKa = 10.78 LFVLAPLLRR28 pKa = 11.84 QFFPAEE34 pKa = 4.09 EE35 pKa = 4.68 SPLSPSTTDD44 pKa = 3.28 YY45 pKa = 11.26 LFPNGADD52 pKa = 3.24 QDD54 pKa = 4.22 ADD56 pKa = 3.9 DD57 pKa = 5.21 LAPSPALNLLDD68 pKa = 4.21 DD69 pKa = 4.24 TFISIDD75 pKa = 3.7 DD76 pKa = 4.59 GVTPDD81 pKa = 4.6 CPHH84 pKa = 6.89 DD85 pKa = 3.58 PAYY88 pKa = 10.51 RR89 pKa = 11.84 IHH91 pKa = 6.79 LLSRR95 pKa = 11.84 TPLVIYY101 pKa = 10.37 IEE103 pKa = 4.82 GFLTPTEE110 pKa = 4.16 ADD112 pKa = 3.29 HH113 pKa = 6.88 LVNII117 pKa = 5.11
Molecular weight: 12.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.745
IPC2_protein 3.923
IPC_protein 3.884
Toseland 3.668
ProMoST 4.075
Dawson 3.897
Bjellqvist 4.062
Wikipedia 3.872
Rodwell 3.719
Grimsley 3.579
Solomon 3.884
Lehninger 3.846
Nozaki 4.024
DTASelect 4.291
Thurlkill 3.745
EMBOSS 3.872
Sillero 4.024
Patrickios 0.896
IPC_peptide 3.884
IPC2_peptide 3.986
IPC2.peptide.svr19 3.894
Protein with the highest isoelectric point:
>tr|A0A421CUH2|A0A421CUH2_9EURO RWD domain-containing protein OS=Aspergillus turcosus OX=1245748 GN=CFD26_101122 PE=3 SV=1
MM1 pKa = 7.18 ISLWRR6 pKa = 11.84 RR7 pKa = 11.84 ILPQRR12 pKa = 11.84 IANTGAQLRR21 pKa = 11.84 DD22 pKa = 3.42 HH23 pKa = 6.41 QANEE27 pKa = 3.99 RR28 pKa = 11.84 TFLSWTRR35 pKa = 11.84 MGLGFAAMALALGRR49 pKa = 11.84 LDD51 pKa = 5.83 AIDD54 pKa = 4.37 GMISLALSTPKK65 pKa = 10.52 ADD67 pKa = 4.38 LLAIPEE73 pKa = 4.21 NPGARR78 pKa = 11.84 TAAAAAAARR87 pKa = 11.84 SGNNSNNSTEE97 pKa = 3.96 QPPNQISAYY106 pKa = 9.24 LASGLSAATLCQAISVWSFGYY127 pKa = 10.89 GIFRR131 pKa = 11.84 YY132 pKa = 10.16 LSVRR136 pKa = 11.84 RR137 pKa = 11.84 NLLKK141 pKa = 10.92 GQFTPAIWGPAFVTCGCLGVFSSLGLWMEE170 pKa = 3.68 QRR172 pKa = 11.84 RR173 pKa = 11.84 DD174 pKa = 3.21 KK175 pKa = 11.36 KK176 pKa = 10.7 MPP178 pKa = 3.9
Molecular weight: 19.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.348
IPC2_protein 9.531
IPC_protein 10.379
Toseland 10.452
ProMoST 10.204
Dawson 10.599
Bjellqvist 10.35
Wikipedia 10.818
Rodwell 10.716
Grimsley 10.657
Solomon 10.701
Lehninger 10.672
Nozaki 10.496
DTASelect 10.321
Thurlkill 10.482
EMBOSS 10.862
Sillero 10.54
Patrickios 10.467
IPC_peptide 10.701
IPC2_peptide 9.604
IPC2.peptide.svr19 8.5
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
9174
0
9174
4845878
50
7333
528.2
58.54
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.648 ± 0.02
1.229 ± 0.01
5.661 ± 0.015
6.259 ± 0.027
3.734 ± 0.015
6.701 ± 0.024
2.384 ± 0.01
4.911 ± 0.017
4.603 ± 0.021
9.141 ± 0.025
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.126 ± 0.009
3.603 ± 0.012
6.004 ± 0.025
4.064 ± 0.015
6.247 ± 0.022
8.263 ± 0.03
5.874 ± 0.017
6.24 ± 0.02
1.467 ± 0.009
2.841 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here