Red clover cryptic virus 2
Average proteome isoelectric point is 5.85
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|M9VYE7|M9VYE7_9VIRU Coat protein OS=Red clover cryptic virus 2 OX=1323524 PE=4 SV=1
MM1 pKa = 7.78 PFNSARR7 pKa = 11.84 NYY9 pKa = 9.61 LAEE12 pKa = 4.08 RR13 pKa = 11.84 MIRR16 pKa = 11.84 TKK18 pKa = 10.58 QEE20 pKa = 3.28 LMTYY24 pKa = 9.46 QSEE27 pKa = 4.35 DD28 pKa = 3.65 HH29 pKa = 6.85 NPDD32 pKa = 4.36 AILEE36 pKa = 4.13 KK37 pKa = 10.91 SQDD40 pKa = 3.15 PDD42 pKa = 3.03 YY43 pKa = 11.22 RR44 pKa = 11.84 RR45 pKa = 11.84 YY46 pKa = 10.46 YY47 pKa = 11.06 DD48 pKa = 3.2 NTRR51 pKa = 11.84 FDD53 pKa = 3.77 PSNEE57 pKa = 3.79 VKK59 pKa = 10.57 HH60 pKa = 6.69 RR61 pKa = 11.84 ILNKK65 pKa = 9.87 EE66 pKa = 3.61 YY67 pKa = 8.91 STLVEE72 pKa = 4.63 AYY74 pKa = 10.04 RR75 pKa = 11.84 IKK77 pKa = 10.69 NDD79 pKa = 3.74 RR80 pKa = 11.84 KK81 pKa = 7.94 HH82 pKa = 5.81 QPYY85 pKa = 9.82 EE86 pKa = 3.6 LHH88 pKa = 6.34 QPIPEE93 pKa = 4.4 DD94 pKa = 3.18 AAPIPEE100 pKa = 4.3 SRR102 pKa = 11.84 VPAPGLKK109 pKa = 9.71 LVPLMYY115 pKa = 10.13 HH116 pKa = 6.24 YY117 pKa = 11.17 GHH119 pKa = 7.04 IVHH122 pKa = 7.24 DD123 pKa = 4.53 PVASEE128 pKa = 5.03 SDD130 pKa = 3.45 SDD132 pKa = 4.29 DD133 pKa = 5.05 DD134 pKa = 3.76 NTASEE139 pKa = 4.76 RR140 pKa = 11.84 PSKK143 pKa = 9.96 TSIPHH148 pKa = 5.97 FGYY151 pKa = 9.76 PVNKK155 pKa = 9.71 RR156 pKa = 11.84 IYY158 pKa = 9.18 DD159 pKa = 3.82 VIVNVYY165 pKa = 9.8 PEE167 pKa = 4.02 YY168 pKa = 10.91 LKK170 pKa = 11.18 VIGEE174 pKa = 4.18 YY175 pKa = 10.04 CRR177 pKa = 11.84 PIGTVEE183 pKa = 3.69 ATFADD188 pKa = 4.64 FNKK191 pKa = 9.91 EE192 pKa = 4.12 QIPSAPINIEE202 pKa = 3.67 RR203 pKa = 11.84 KK204 pKa = 8.71 EE205 pKa = 3.92 QVLTHH210 pKa = 6.44 IFKK213 pKa = 10.77 FLDD216 pKa = 3.75 AQPYY220 pKa = 9.73 LPLHH224 pKa = 5.98 FVDD227 pKa = 4.11 TQFCKK232 pKa = 10.39 TPLVTGTGYY241 pKa = 10.81 HH242 pKa = 5.59 NRR244 pKa = 11.84 YY245 pKa = 9.47 SFKK248 pKa = 10.71 QKK250 pKa = 10.44 AHH252 pKa = 6.02 AKK254 pKa = 8.37 YY255 pKa = 10.37 SRR257 pKa = 11.84 PEE259 pKa = 3.92 EE260 pKa = 4.18 YY261 pKa = 10.83 AKK263 pKa = 10.98 LPTSKK268 pKa = 10.77 GYY270 pKa = 10.32 FYY272 pKa = 11.19 NATYY276 pKa = 10.79 EE277 pKa = 3.99 NARR280 pKa = 11.84 TLVHH284 pKa = 7.13 FIKK287 pKa = 10.82 QFGLPFNLQYY297 pKa = 11.12 APEE300 pKa = 4.85 DD301 pKa = 3.76 ADD303 pKa = 3.67 PTDD306 pKa = 3.97 EE307 pKa = 4.44 QVQSYY312 pKa = 10.59 IDD314 pKa = 3.46 TANSFFNDD322 pKa = 3.36 YY323 pKa = 7.74 PTLLFTRR330 pKa = 11.84 NHH332 pKa = 5.43 ISKK335 pKa = 10.37 RR336 pKa = 11.84 DD337 pKa = 3.44 GTLKK341 pKa = 10.16 VRR343 pKa = 11.84 PVYY346 pKa = 10.61 AVDD349 pKa = 3.86 DD350 pKa = 4.04 LFIIIEE356 pKa = 4.15 LMLTFPLTVQARR368 pKa = 11.84 KK369 pKa = 9.5 QSCCIMYY376 pKa = 10.47 GLEE379 pKa = 4.17 TIRR382 pKa = 11.84 GSNHH386 pKa = 5.57 YY387 pKa = 9.72 IEE389 pKa = 5.15 RR390 pKa = 11.84 LARR393 pKa = 11.84 SYY395 pKa = 10.05 STYY398 pKa = 10.64 FSLDD402 pKa = 2.68 WSSYY406 pKa = 6.99 DD407 pKa = 3.17 QRR409 pKa = 11.84 LPRR412 pKa = 11.84 VITDD416 pKa = 3.3 IYY418 pKa = 9.53 YY419 pKa = 9.37 TDD421 pKa = 3.94 FLRR424 pKa = 11.84 RR425 pKa = 11.84 LIVINHH431 pKa = 7.13 GYY433 pKa = 9.18 QPTYY437 pKa = 10.39 EE438 pKa = 4.22 YY439 pKa = 8.37 PTYY442 pKa = 10.44 PDD444 pKa = 3.72 LDD446 pKa = 3.56 EE447 pKa = 5.2 HH448 pKa = 7.08 KK449 pKa = 10.58 LYY451 pKa = 11.33 SRR453 pKa = 11.84 MNNLLYY459 pKa = 10.34 FLHH462 pKa = 6.11 TWYY465 pKa = 11.33 NNMTFVLSDD474 pKa = 3.4 GYY476 pKa = 11.22 AYY478 pKa = 10.65 RR479 pKa = 11.84 RR480 pKa = 11.84 THH482 pKa = 6.4 CGVPSGLYY490 pKa = 7.56 NTQYY494 pKa = 11.31 LDD496 pKa = 3.35 SFGNLFLIIDD506 pKa = 4.03 AMLEE510 pKa = 4.09 FGFSEE515 pKa = 4.57 SEE517 pKa = 3.34 IDD519 pKa = 4.26 NFILLVLGDD528 pKa = 5.24 DD529 pKa = 3.95 NTGMTVISIDD539 pKa = 4.12 RR540 pKa = 11.84 IYY542 pKa = 11.38 DD543 pKa = 3.97 FINFLEE549 pKa = 4.62 KK550 pKa = 10.68 YY551 pKa = 10.59 ALIRR555 pKa = 11.84 YY556 pKa = 8.82 NMVLSPTKK564 pKa = 10.56 SVLTTLRR571 pKa = 11.84 SKK573 pKa = 10.73 IEE575 pKa = 3.91 TLGYY579 pKa = 9.27 QCNHH583 pKa = 6.68 GSPKK587 pKa = 9.95 RR588 pKa = 11.84 DD589 pKa = 2.91 ISKK592 pKa = 10.59 LVAQLCYY599 pKa = 9.98 PEE601 pKa = 5.79 NGLKK605 pKa = 9.45 PHH607 pKa = 6.12 TMAARR612 pKa = 11.84 AIGIAYY618 pKa = 8.9 AAAGQDD624 pKa = 3.48 PMFHH628 pKa = 6.27 SFCHH632 pKa = 6.17 DD633 pKa = 3.26 VYY635 pKa = 11.72 NLFRR639 pKa = 11.84 LDD641 pKa = 3.9 YY642 pKa = 10.95 KK643 pKa = 10.9 PDD645 pKa = 3.05 ARR647 pKa = 11.84 TNLNFQRR654 pKa = 11.84 QIYY657 pKa = 9.6 HH658 pKa = 6.09 NLEE661 pKa = 4.32 DD662 pKa = 5.48 GIPDD666 pKa = 4.25 LATPVVPPFPSLYY679 pKa = 9.46 EE680 pKa = 3.88 VRR682 pKa = 11.84 HH683 pKa = 5.87 MYY685 pKa = 10.45 SKK687 pKa = 10.62 YY688 pKa = 10.07 QGPLSYY694 pKa = 10.73 APKK697 pKa = 9.2 WNYY700 pKa = 10.18 AHH702 pKa = 7.53 FINDD706 pKa = 3.81 PDD708 pKa = 3.9 VTPPSPKK715 pKa = 9.65 TMRR718 pKa = 11.84 DD719 pKa = 3.43 YY720 pKa = 11.07 EE721 pKa = 4.24 IEE723 pKa = 3.97 NDD725 pKa = 4.35 LISRR729 pKa = 11.84 TAPTFEE735 pKa = 4.15 TVVPATRR742 pKa = 11.84 NFPP745 pKa = 3.73
Molecular weight: 86.76 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.67
IPC2_protein 5.753
IPC_protein 5.83
Toseland 6.313
ProMoST 6.211
Dawson 6.122
Bjellqvist 6.122
Wikipedia 6.148
Rodwell 6.109
Grimsley 6.504
Solomon 6.122
Lehninger 6.122
Nozaki 6.402
DTASelect 6.59
Thurlkill 6.605
EMBOSS 6.576
Sillero 6.504
Patrickios 3.3
IPC_peptide 6.135
IPC2_peptide 6.491
IPC2.peptide.svr19 6.409
Protein with the highest isoelectric point:
>tr|M9VYE7|M9VYE7_9VIRU Coat protein OS=Red clover cryptic virus 2 OX=1323524 PE=4 SV=1
MM1 pKa = 6.97 STEE4 pKa = 3.85 EE5 pKa = 4.24 TLPPTKK11 pKa = 9.94 TIKK14 pKa = 9.92 NQNVNLIFQDD24 pKa = 3.51 TKK26 pKa = 11.44 VPIPSDD32 pKa = 2.97 NFMEE36 pKa = 4.57 LLRR39 pKa = 11.84 SNAAAHH45 pKa = 6.57 TDD47 pKa = 3.46 TSNLWVIDD55 pKa = 3.56 VLPNFTPILMFIMHH69 pKa = 7.06 YY70 pKa = 10.9 AHH72 pKa = 7.0 IHH74 pKa = 5.2 AQAADD79 pKa = 3.66 YY80 pKa = 10.16 RR81 pKa = 11.84 VHH83 pKa = 6.82 AKK85 pKa = 10.26 SSAYY89 pKa = 7.55 TLCMYY94 pKa = 11.06 YY95 pKa = 7.67 MTVVYY100 pKa = 9.74 GYY102 pKa = 10.96 FLLNDD107 pKa = 3.84 LHH109 pKa = 6.1 TRR111 pKa = 11.84 PTRR114 pKa = 11.84 SVHH117 pKa = 4.6 ARR119 pKa = 11.84 VWVEE123 pKa = 3.69 SSWRR127 pKa = 11.84 SDD129 pKa = 3.16 FVKK132 pKa = 10.44 FLTTLPVPEE141 pKa = 4.3 FLKK144 pKa = 10.88 PILSQFHH151 pKa = 6.96 PFNTDD156 pKa = 2.27 RR157 pKa = 11.84 TKK159 pKa = 11.26 NVFFSPSAAGFDD171 pKa = 3.32 HH172 pKa = 7.28 DD173 pKa = 4.43 QFFGRR178 pKa = 11.84 IFPMNMFAAFHH189 pKa = 7.31 DD190 pKa = 4.61 CTATLPGNSSKK201 pKa = 10.95 VDD203 pKa = 3.41 VLRR206 pKa = 11.84 NLYY209 pKa = 8.6 TRR211 pKa = 11.84 NLYY214 pKa = 9.93 TITAPGYY221 pKa = 7.97 TCMIPDD227 pKa = 5.67 LIGITPDD234 pKa = 2.81 QAAATTVNYY243 pKa = 8.75 MNSKK247 pKa = 10.02 FYY249 pKa = 10.03 QTFTSIFNPVLFRR262 pKa = 11.84 DD263 pKa = 3.86 SQRR266 pKa = 11.84 RR267 pKa = 11.84 ASLAALSFTAPTYY280 pKa = 7.37 PTNHH284 pKa = 5.98 INAYY288 pKa = 10.43 DD289 pKa = 3.76 YY290 pKa = 10.03 MFSATAPNLRR300 pKa = 11.84 EE301 pKa = 3.95 LRR303 pKa = 11.84 IVLQSIIAITKK314 pKa = 10.12 DD315 pKa = 3.35 VFKK318 pKa = 11.06 SDD320 pKa = 3.61 QTLGNYY326 pKa = 8.2 VADD329 pKa = 3.93 YY330 pKa = 10.55 SSGSIIHH337 pKa = 6.59 HH338 pKa = 6.77 GYY340 pKa = 7.78 STYY343 pKa = 11.42 ALPTWSHH350 pKa = 6.09 TPTTGKK356 pKa = 10.06 FDD358 pKa = 3.91 RR359 pKa = 11.84 FNTANTFNLVTEE371 pKa = 4.51 DD372 pKa = 4.02 ARR374 pKa = 11.84 AEE376 pKa = 4.5 DD377 pKa = 3.42 ICFLQRR383 pKa = 11.84 PAAEE387 pKa = 4.24 IPHH390 pKa = 6.24 TRR392 pKa = 11.84 LITDD396 pKa = 3.76 IVYY399 pKa = 10.39 AATTAPATSLTVPANHH415 pKa = 6.79 ALYY418 pKa = 10.44 RR419 pKa = 11.84 RR420 pKa = 11.84 WPYY423 pKa = 10.69 CLRR426 pKa = 11.84 LDD428 pKa = 3.75 EE429 pKa = 5.17 DD430 pKa = 4.07 AANGFPRR437 pKa = 11.84 HH438 pKa = 6.25 DD439 pKa = 4.23 NEE441 pKa = 4.89 DD442 pKa = 3.34 LVAFSAEE449 pKa = 3.73 SHH451 pKa = 5.94 AAPRR455 pKa = 11.84 VLVLDD460 pKa = 4.05 TPGDD464 pKa = 4.07 TVTSAHH470 pKa = 6.69 LPTLTGKK477 pKa = 10.53 VIEE480 pKa = 4.35 SFEE483 pKa = 4.76 LDD485 pKa = 3.07 GSTIEE490 pKa = 4.42 MPDD493 pKa = 3.13 ARR495 pKa = 11.84 KK496 pKa = 10.04 SLGMQNCMFADD507 pKa = 3.4 SAIAYY512 pKa = 8.89 KK513 pKa = 9.56 YY514 pKa = 9.9 VRR516 pKa = 11.84 PGSYY520 pKa = 8.67 WRR522 pKa = 11.84 PRR524 pKa = 11.84 AAGGTLPPLNRR535 pKa = 11.84 APPNSRR541 pKa = 11.84 PRR543 pKa = 11.84 LPASSLLHH551 pKa = 6.91 DD552 pKa = 3.74 RR553 pKa = 11.84 TKK555 pKa = 11.14 VFLPQLNRR563 pKa = 11.84 HH564 pKa = 5.06 VNAVPDD570 pKa = 3.78 NDD572 pKa = 4.04 ALPGFTQITPVNVIRR587 pKa = 11.84 YY588 pKa = 7.25 IQSFLGFRR596 pKa = 11.84 TVDD599 pKa = 3.14 SSANEE604 pKa = 3.86 AALDD608 pKa = 3.78 AVPGMNEE615 pKa = 3.99 SLLMIWSPYY624 pKa = 8.28 TYY626 pKa = 10.77 NPYY629 pKa = 10.77 EE630 pKa = 4.54 SDD632 pKa = 4.28 DD633 pKa = 4.15 YY634 pKa = 11.46 PALAYY639 pKa = 9.46 DD640 pKa = 4.28 ASRR643 pKa = 11.84 HH644 pKa = 5.13 YY645 pKa = 11.23 YY646 pKa = 8.29 LTNLRR651 pKa = 11.84 TIFGTDD657 pKa = 3.27 YY658 pKa = 11.17 NLVATKK664 pKa = 10.12 HH665 pKa = 6.0 IYY667 pKa = 10.35 EE668 pKa = 4.55 SFPAVV673 pKa = 3.04
Molecular weight: 75.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.034
IPC2_protein 6.008
IPC_protein 6.122
Toseland 6.605
ProMoST 6.532
Dawson 6.465
Bjellqvist 6.44
Wikipedia 6.491
Rodwell 6.453
Grimsley 6.795
Solomon 6.465
Lehninger 6.465
Nozaki 6.751
DTASelect 6.942
Thurlkill 6.971
EMBOSS 6.942
Sillero 6.854
Patrickios 3.668
IPC_peptide 6.478
IPC2_peptide 6.81
IPC2.peptide.svr19 6.75
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1418
673
745
709.0
81.22
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.546 ± 1.567
0.987 ± 0.066
6.417 ± 0.225
4.09 ± 0.878
5.43 ± 0.253
3.456 ± 0.129
3.456 ± 0.026
5.712 ± 0.457
3.597 ± 0.742
8.533 ± 0.059
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.186 ± 0.133
5.642 ± 0.106
7.475 ± 0.032
2.821 ± 0.41
5.642 ± 0.1
6.488 ± 0.446
7.828 ± 1.062
5.289 ± 0.248
0.705 ± 0.232
6.7 ± 1.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here