Red clover cryptic virus 2

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Duplopiviricetes; Durnavirales; Partitiviridae; Betapartitivirus

Average proteome isoelectric point is 5.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|M9VYE7|M9VYE7_9VIRU Coat protein OS=Red clover cryptic virus 2 OX=1323524 PE=4 SV=1
MM1 pKa = 7.78PFNSARR7 pKa = 11.84NYY9 pKa = 9.61LAEE12 pKa = 4.08RR13 pKa = 11.84MIRR16 pKa = 11.84TKK18 pKa = 10.58QEE20 pKa = 3.28LMTYY24 pKa = 9.46QSEE27 pKa = 4.35DD28 pKa = 3.65HH29 pKa = 6.85NPDD32 pKa = 4.36AILEE36 pKa = 4.13KK37 pKa = 10.91SQDD40 pKa = 3.15PDD42 pKa = 3.03YY43 pKa = 11.22RR44 pKa = 11.84RR45 pKa = 11.84YY46 pKa = 10.46YY47 pKa = 11.06DD48 pKa = 3.2NTRR51 pKa = 11.84FDD53 pKa = 3.77PSNEE57 pKa = 3.79VKK59 pKa = 10.57HH60 pKa = 6.69RR61 pKa = 11.84ILNKK65 pKa = 9.87EE66 pKa = 3.61YY67 pKa = 8.91STLVEE72 pKa = 4.63AYY74 pKa = 10.04RR75 pKa = 11.84IKK77 pKa = 10.69NDD79 pKa = 3.74RR80 pKa = 11.84KK81 pKa = 7.94HH82 pKa = 5.81QPYY85 pKa = 9.82EE86 pKa = 3.6LHH88 pKa = 6.34QPIPEE93 pKa = 4.4DD94 pKa = 3.18AAPIPEE100 pKa = 4.3SRR102 pKa = 11.84VPAPGLKK109 pKa = 9.71LVPLMYY115 pKa = 10.13HH116 pKa = 6.24YY117 pKa = 11.17GHH119 pKa = 7.04IVHH122 pKa = 7.24DD123 pKa = 4.53PVASEE128 pKa = 5.03SDD130 pKa = 3.45SDD132 pKa = 4.29DD133 pKa = 5.05DD134 pKa = 3.76NTASEE139 pKa = 4.76RR140 pKa = 11.84PSKK143 pKa = 9.96TSIPHH148 pKa = 5.97FGYY151 pKa = 9.76PVNKK155 pKa = 9.71RR156 pKa = 11.84IYY158 pKa = 9.18DD159 pKa = 3.82VIVNVYY165 pKa = 9.8PEE167 pKa = 4.02YY168 pKa = 10.91LKK170 pKa = 11.18VIGEE174 pKa = 4.18YY175 pKa = 10.04CRR177 pKa = 11.84PIGTVEE183 pKa = 3.69ATFADD188 pKa = 4.64FNKK191 pKa = 9.91EE192 pKa = 4.12QIPSAPINIEE202 pKa = 3.67RR203 pKa = 11.84KK204 pKa = 8.71EE205 pKa = 3.92QVLTHH210 pKa = 6.44IFKK213 pKa = 10.77FLDD216 pKa = 3.75AQPYY220 pKa = 9.73LPLHH224 pKa = 5.98FVDD227 pKa = 4.11TQFCKK232 pKa = 10.39TPLVTGTGYY241 pKa = 10.81HH242 pKa = 5.59NRR244 pKa = 11.84YY245 pKa = 9.47SFKK248 pKa = 10.71QKK250 pKa = 10.44AHH252 pKa = 6.02AKK254 pKa = 8.37YY255 pKa = 10.37SRR257 pKa = 11.84PEE259 pKa = 3.92EE260 pKa = 4.18YY261 pKa = 10.83AKK263 pKa = 10.98LPTSKK268 pKa = 10.77GYY270 pKa = 10.32FYY272 pKa = 11.19NATYY276 pKa = 10.79EE277 pKa = 3.99NARR280 pKa = 11.84TLVHH284 pKa = 7.13FIKK287 pKa = 10.82QFGLPFNLQYY297 pKa = 11.12APEE300 pKa = 4.85DD301 pKa = 3.76ADD303 pKa = 3.67PTDD306 pKa = 3.97EE307 pKa = 4.44QVQSYY312 pKa = 10.59IDD314 pKa = 3.46TANSFFNDD322 pKa = 3.36YY323 pKa = 7.74PTLLFTRR330 pKa = 11.84NHH332 pKa = 5.43ISKK335 pKa = 10.37RR336 pKa = 11.84DD337 pKa = 3.44GTLKK341 pKa = 10.16VRR343 pKa = 11.84PVYY346 pKa = 10.61AVDD349 pKa = 3.86DD350 pKa = 4.04LFIIIEE356 pKa = 4.15LMLTFPLTVQARR368 pKa = 11.84KK369 pKa = 9.5QSCCIMYY376 pKa = 10.47GLEE379 pKa = 4.17TIRR382 pKa = 11.84GSNHH386 pKa = 5.57YY387 pKa = 9.72IEE389 pKa = 5.15RR390 pKa = 11.84LARR393 pKa = 11.84SYY395 pKa = 10.05STYY398 pKa = 10.64FSLDD402 pKa = 2.68WSSYY406 pKa = 6.99DD407 pKa = 3.17QRR409 pKa = 11.84LPRR412 pKa = 11.84VITDD416 pKa = 3.3IYY418 pKa = 9.53YY419 pKa = 9.37TDD421 pKa = 3.94FLRR424 pKa = 11.84RR425 pKa = 11.84LIVINHH431 pKa = 7.13GYY433 pKa = 9.18QPTYY437 pKa = 10.39EE438 pKa = 4.22YY439 pKa = 8.37PTYY442 pKa = 10.44PDD444 pKa = 3.72LDD446 pKa = 3.56EE447 pKa = 5.2HH448 pKa = 7.08KK449 pKa = 10.58LYY451 pKa = 11.33SRR453 pKa = 11.84MNNLLYY459 pKa = 10.34FLHH462 pKa = 6.11TWYY465 pKa = 11.33NNMTFVLSDD474 pKa = 3.4GYY476 pKa = 11.22AYY478 pKa = 10.65RR479 pKa = 11.84RR480 pKa = 11.84THH482 pKa = 6.4CGVPSGLYY490 pKa = 7.56NTQYY494 pKa = 11.31LDD496 pKa = 3.35SFGNLFLIIDD506 pKa = 4.03AMLEE510 pKa = 4.09FGFSEE515 pKa = 4.57SEE517 pKa = 3.34IDD519 pKa = 4.26NFILLVLGDD528 pKa = 5.24DD529 pKa = 3.95NTGMTVISIDD539 pKa = 4.12RR540 pKa = 11.84IYY542 pKa = 11.38DD543 pKa = 3.97FINFLEE549 pKa = 4.62KK550 pKa = 10.68YY551 pKa = 10.59ALIRR555 pKa = 11.84YY556 pKa = 8.82NMVLSPTKK564 pKa = 10.56SVLTTLRR571 pKa = 11.84SKK573 pKa = 10.73IEE575 pKa = 3.91TLGYY579 pKa = 9.27QCNHH583 pKa = 6.68GSPKK587 pKa = 9.95RR588 pKa = 11.84DD589 pKa = 2.91ISKK592 pKa = 10.59LVAQLCYY599 pKa = 9.98PEE601 pKa = 5.79NGLKK605 pKa = 9.45PHH607 pKa = 6.12TMAARR612 pKa = 11.84AIGIAYY618 pKa = 8.9AAAGQDD624 pKa = 3.48PMFHH628 pKa = 6.27SFCHH632 pKa = 6.17DD633 pKa = 3.26VYY635 pKa = 11.72NLFRR639 pKa = 11.84LDD641 pKa = 3.9YY642 pKa = 10.95KK643 pKa = 10.9PDD645 pKa = 3.05ARR647 pKa = 11.84TNLNFQRR654 pKa = 11.84QIYY657 pKa = 9.6HH658 pKa = 6.09NLEE661 pKa = 4.32DD662 pKa = 5.48GIPDD666 pKa = 4.25LATPVVPPFPSLYY679 pKa = 9.46EE680 pKa = 3.88VRR682 pKa = 11.84HH683 pKa = 5.87MYY685 pKa = 10.45SKK687 pKa = 10.62YY688 pKa = 10.07QGPLSYY694 pKa = 10.73APKK697 pKa = 9.2WNYY700 pKa = 10.18AHH702 pKa = 7.53FINDD706 pKa = 3.81PDD708 pKa = 3.9VTPPSPKK715 pKa = 9.65TMRR718 pKa = 11.84DD719 pKa = 3.43YY720 pKa = 11.07EE721 pKa = 4.24IEE723 pKa = 3.97NDD725 pKa = 4.35LISRR729 pKa = 11.84TAPTFEE735 pKa = 4.15TVVPATRR742 pKa = 11.84NFPP745 pKa = 3.73

Molecular weight:
86.76 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|M9VYE7|M9VYE7_9VIRU Coat protein OS=Red clover cryptic virus 2 OX=1323524 PE=4 SV=1
MM1 pKa = 6.97STEE4 pKa = 3.85EE5 pKa = 4.24TLPPTKK11 pKa = 9.94TIKK14 pKa = 9.92NQNVNLIFQDD24 pKa = 3.51TKK26 pKa = 11.44VPIPSDD32 pKa = 2.97NFMEE36 pKa = 4.57LLRR39 pKa = 11.84SNAAAHH45 pKa = 6.57TDD47 pKa = 3.46TSNLWVIDD55 pKa = 3.56VLPNFTPILMFIMHH69 pKa = 7.06YY70 pKa = 10.9AHH72 pKa = 7.0IHH74 pKa = 5.2AQAADD79 pKa = 3.66YY80 pKa = 10.16RR81 pKa = 11.84VHH83 pKa = 6.82AKK85 pKa = 10.26SSAYY89 pKa = 7.55TLCMYY94 pKa = 11.06YY95 pKa = 7.67MTVVYY100 pKa = 9.74GYY102 pKa = 10.96FLLNDD107 pKa = 3.84LHH109 pKa = 6.1TRR111 pKa = 11.84PTRR114 pKa = 11.84SVHH117 pKa = 4.6ARR119 pKa = 11.84VWVEE123 pKa = 3.69SSWRR127 pKa = 11.84SDD129 pKa = 3.16FVKK132 pKa = 10.44FLTTLPVPEE141 pKa = 4.3FLKK144 pKa = 10.88PILSQFHH151 pKa = 6.96PFNTDD156 pKa = 2.27RR157 pKa = 11.84TKK159 pKa = 11.26NVFFSPSAAGFDD171 pKa = 3.32HH172 pKa = 7.28DD173 pKa = 4.43QFFGRR178 pKa = 11.84IFPMNMFAAFHH189 pKa = 7.31DD190 pKa = 4.61CTATLPGNSSKK201 pKa = 10.95VDD203 pKa = 3.41VLRR206 pKa = 11.84NLYY209 pKa = 8.6TRR211 pKa = 11.84NLYY214 pKa = 9.93TITAPGYY221 pKa = 7.97TCMIPDD227 pKa = 5.67LIGITPDD234 pKa = 2.81QAAATTVNYY243 pKa = 8.75MNSKK247 pKa = 10.02FYY249 pKa = 10.03QTFTSIFNPVLFRR262 pKa = 11.84DD263 pKa = 3.86SQRR266 pKa = 11.84RR267 pKa = 11.84ASLAALSFTAPTYY280 pKa = 7.37PTNHH284 pKa = 5.98INAYY288 pKa = 10.43DD289 pKa = 3.76YY290 pKa = 10.03MFSATAPNLRR300 pKa = 11.84EE301 pKa = 3.95LRR303 pKa = 11.84IVLQSIIAITKK314 pKa = 10.12DD315 pKa = 3.35VFKK318 pKa = 11.06SDD320 pKa = 3.61QTLGNYY326 pKa = 8.2VADD329 pKa = 3.93YY330 pKa = 10.55SSGSIIHH337 pKa = 6.59HH338 pKa = 6.77GYY340 pKa = 7.78STYY343 pKa = 11.42ALPTWSHH350 pKa = 6.09TPTTGKK356 pKa = 10.06FDD358 pKa = 3.91RR359 pKa = 11.84FNTANTFNLVTEE371 pKa = 4.51DD372 pKa = 4.02ARR374 pKa = 11.84AEE376 pKa = 4.5DD377 pKa = 3.42ICFLQRR383 pKa = 11.84PAAEE387 pKa = 4.24IPHH390 pKa = 6.24TRR392 pKa = 11.84LITDD396 pKa = 3.76IVYY399 pKa = 10.39AATTAPATSLTVPANHH415 pKa = 6.79ALYY418 pKa = 10.44RR419 pKa = 11.84RR420 pKa = 11.84WPYY423 pKa = 10.69CLRR426 pKa = 11.84LDD428 pKa = 3.75EE429 pKa = 5.17DD430 pKa = 4.07AANGFPRR437 pKa = 11.84HH438 pKa = 6.25DD439 pKa = 4.23NEE441 pKa = 4.89DD442 pKa = 3.34LVAFSAEE449 pKa = 3.73SHH451 pKa = 5.94AAPRR455 pKa = 11.84VLVLDD460 pKa = 4.05TPGDD464 pKa = 4.07TVTSAHH470 pKa = 6.69LPTLTGKK477 pKa = 10.53VIEE480 pKa = 4.35SFEE483 pKa = 4.76LDD485 pKa = 3.07GSTIEE490 pKa = 4.42MPDD493 pKa = 3.13ARR495 pKa = 11.84KK496 pKa = 10.04SLGMQNCMFADD507 pKa = 3.4SAIAYY512 pKa = 8.89KK513 pKa = 9.56YY514 pKa = 9.9VRR516 pKa = 11.84PGSYY520 pKa = 8.67WRR522 pKa = 11.84PRR524 pKa = 11.84AAGGTLPPLNRR535 pKa = 11.84APPNSRR541 pKa = 11.84PRR543 pKa = 11.84LPASSLLHH551 pKa = 6.91DD552 pKa = 3.74RR553 pKa = 11.84TKK555 pKa = 11.14VFLPQLNRR563 pKa = 11.84HH564 pKa = 5.06VNAVPDD570 pKa = 3.78NDD572 pKa = 4.04ALPGFTQITPVNVIRR587 pKa = 11.84YY588 pKa = 7.25IQSFLGFRR596 pKa = 11.84TVDD599 pKa = 3.14SSANEE604 pKa = 3.86AALDD608 pKa = 3.78AVPGMNEE615 pKa = 3.99SLLMIWSPYY624 pKa = 8.28TYY626 pKa = 10.77NPYY629 pKa = 10.77EE630 pKa = 4.54SDD632 pKa = 4.28DD633 pKa = 4.15YY634 pKa = 11.46PALAYY639 pKa = 9.46DD640 pKa = 4.28ASRR643 pKa = 11.84HH644 pKa = 5.13YY645 pKa = 11.23YY646 pKa = 8.29LTNLRR651 pKa = 11.84TIFGTDD657 pKa = 3.27YY658 pKa = 11.17NLVATKK664 pKa = 10.12HH665 pKa = 6.0IYY667 pKa = 10.35EE668 pKa = 4.55SFPAVV673 pKa = 3.04

Molecular weight:
75.68 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1418

673

745

709.0

81.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.546 ± 1.567

0.987 ± 0.066

6.417 ± 0.225

4.09 ± 0.878

5.43 ± 0.253

3.456 ± 0.129

3.456 ± 0.026

5.712 ± 0.457

3.597 ± 0.742

8.533 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.186 ± 0.133

5.642 ± 0.106

7.475 ± 0.032

2.821 ± 0.41

5.642 ± 0.1

6.488 ± 0.446

7.828 ± 1.062

5.289 ± 0.248

0.705 ± 0.232

6.7 ± 1.039

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski