Malus domestica (Apple) (Pyrus malus)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae;

Average proteome isoelectric point is 6.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 42478 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A498HRZ1|A0A498HRZ1_MALDO Uncharacterized protein OS=Malus domestica OX=3750 GN=DVH24_029039 PE=4 SV=1
MM1 pKa = 7.57GGGGGGGYY9 pKa = 10.49GDD11 pKa = 4.77EE12 pKa = 4.94DD13 pKa = 4.97CGDD16 pKa = 3.71LLGNDD21 pKa = 4.18VYY23 pKa = 11.77NNRR26 pKa = 11.84CPEE29 pKa = 4.06AEE31 pKa = 4.37AIIYY35 pKa = 9.02AAAGGCDD42 pKa = 3.59ASALLDD48 pKa = 4.23DD49 pKa = 4.87NEE51 pKa = 5.05NIVGAHH57 pKa = 4.91TMGKK61 pKa = 9.62ARR63 pKa = 11.84CTTFTARR70 pKa = 11.84LQGSTTALEE79 pKa = 4.42YY80 pKa = 10.44IQLLRR85 pKa = 11.84QLCSEE90 pKa = 4.15SDD92 pKa = 3.3INTLANLDD100 pKa = 4.1LATPATFDD108 pKa = 3.29DD109 pKa = 4.16QYY111 pKa = 11.73YY112 pKa = 11.07LNLLSGEE119 pKa = 4.31GLLLSDD125 pKa = 4.59QNITTRR131 pKa = 11.84DD132 pKa = 3.53DD133 pKa = 3.3QTRR136 pKa = 11.84EE137 pKa = 3.93LVEE140 pKa = 4.91TYY142 pKa = 10.64AQDD145 pKa = 3.3PSAFFGDD152 pKa = 4.78FKK154 pKa = 11.55DD155 pKa = 4.19SMVKK159 pKa = 9.36MGRR162 pKa = 11.84VEE164 pKa = 3.5AWEE167 pKa = 3.85

Molecular weight: 17.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A498K4B5|A0A498K4B5_MALDO Uncharacterized protein OS=Malus domestica OX=3750 GN=DVH24_000737 PE=4 SV=1
MM1 pKa = 7.79ANRR4 pKa = 11.84TGHH7 pKa = 6.86PGGGYY12 pKa = 9.2PPPQQVVQPQKK23 pKa = 9.93PKK25 pKa = 10.02RR26 pKa = 11.84GGGGRR31 pKa = 11.84QQVVQRR37 pKa = 11.84QQVVQRR43 pKa = 11.84QQVVQRR49 pKa = 11.84QQVVQRR55 pKa = 11.84QQVVPRR61 pKa = 11.84QQVVQRR67 pKa = 11.84QQVVQRR73 pKa = 11.84QQVAQQQQVAPRR85 pKa = 11.84QQVVQRR91 pKa = 11.84QQVVQRR97 pKa = 11.84QQVVQRR103 pKa = 11.84QQVVQRR109 pKa = 11.84QQVVQRR115 pKa = 11.84QQVVQRR121 pKa = 11.84QQVVQRR127 pKa = 11.84QQVVQRR133 pKa = 11.84QQVVQRR139 pKa = 11.84QQVVQRR145 pKa = 11.84QQVVPRR151 pKa = 11.84QQVVQVLQLQQVVQVVQAQKK171 pKa = 10.38PKK173 pKa = 10.59RR174 pKa = 11.84GGG176 pKa = 3.19

Molecular weight: 20.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

42388

90

42478

16694911

8

5393

393.0

43.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.773 ± 0.01

1.885 ± 0.006

5.21 ± 0.009

6.382 ± 0.015

4.23 ± 0.008

6.622 ± 0.011

2.443 ± 0.005

5.105 ± 0.008

6.029 ± 0.012

9.704 ± 0.017

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.401 ± 0.005

4.396 ± 0.008

5.07 ± 0.012

3.707 ± 0.008

5.385 ± 0.01

9.037 ± 0.013

4.973 ± 0.007

6.612 ± 0.009

1.291 ± 0.004

2.745 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski