Roseivivax sediminis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Roseivivax

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4371 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I2DWI9|A0A1I2DWI9_9RHOB Alpha-D-ribose 1-methylphosphonate 5-triphosphate diphosphatase OS=Roseivivax sediminis OX=936889 GN=SAMN04515678_11913 PE=4 SV=1
DDD2 pKa = 4.11EE3 pKa = 5.53DD4 pKa = 4.13GYYY7 pKa = 10.01PPNVAPEEE15 pKa = 4.02ADDD18 pKa = 3.76DD19 pKa = 4.3FATPEEE25 pKa = 3.99DD26 pKa = 3.72ALEEE30 pKa = 4.02TAAALLDDD38 pKa = 3.93DDD40 pKa = 4.07DDD42 pKa = 4.72NGDDD46 pKa = 3.87LEEE49 pKa = 4.36LSVTAPAHHH58 pKa = 5.08TLTGTASGWLYYY70 pKa = 10.54PAAGYYY76 pKa = 10.0GPDDD80 pKa = 3.13FTYYY84 pKa = 9.95VGDDD88 pKa = 3.67TATDDD93 pKa = 3.78ATVAVMVGEEE103 pKa = 4.71GAVSDDD109 pKa = 4.76DD110 pKa = 3.84ASGEEE115 pKa = 4.44DDD117 pKa = 3.7AWVFQGIAGSAGLGYYY133 pKa = 10.64GPDDD137 pKa = 3.28LATITSPEEE146 pKa = 4.16VKKK149 pKa = 8.89TAADDD154 pKa = 3.51LTAPRR159 pKa = 11.84LTQAVLDDD167 pKa = 4.25DDD169 pKa = 4.17QISAGFLSEEE179 pKa = 4.66SQQFQEEE186 pKa = 4.33HH187 pKa = 6.29LLVIEEE193 pKa = 6.08DD194 pKa = 3.68EE195 pKa = 4.67NWIRR199 pKa = 11.84FDDD202 pKa = 4.2AYYY205 pKa = 9.96GDDD208 pKa = 4.06LSLIVGTIEEE218 pKa = 4.11GSTTFPLFQTVPSGTVEEE236 pKa = 4.0EE237 pKa = 4.6RR238 pKa = 11.84VTRR241 pKa = 11.84VGDDD245 pKa = 3.32FTFEEE250 pKa = 4.3RR251 pKa = 11.84GGGSSDDD258 pKa = 2.85AQVFSLVRR266 pKa = 11.84AMDDD270 pKa = 3.52TEEE273 pKa = 4.15GVFAGSTSYYY283 pKa = 9.49TDDD286 pKa = 3.1PGYYY290 pKa = 7.24ALVDDD295 pKa = 4.03YY296 pKa = 10.27EEE298 pKa = 5.16AADDD302 pKa = 4.14IGIEEE307 pKa = 4.34DD308 pKa = 4.15SYYY311 pKa = 10.61PQNHHH316 pKa = 6.42PVAVDDD322 pKa = 4.43DD323 pKa = 4.48FSGSGAIVLSKKK335 pKa = 11.14DDD337 pKa = 4.32LANDDD342 pKa = 4.35DDD344 pKa = 4.36DDD346 pKa = 4.32DDD348 pKa = 4.17DD349 pKa = 4.32LSVTLAGPASHHH361 pKa = 6.96SAIDDD366 pKa = 3.75GDDD369 pKa = 3.18TITYYY374 pKa = 9.02PDDD377 pKa = 3.09DDD379 pKa = 4.16EEE381 pKa = 4.48TDDD384 pKa = 3.14FSYYY388 pKa = 10.51LSDDD392 pKa = 3.56TATDDD397 pKa = 3.88EE398 pKa = 3.92WVYYY402 pKa = 11.19DDD404 pKa = 3.48VPVIEEE410 pKa = 4.1WYYY413 pKa = 10.82DDD415 pKa = 3.63QTFGSPGEEE424 pKa = 4.08QTWINILGNVSAEEE438 pKa = 4.16TEEE441 pKa = 5.28SYYY444 pKa = 10.73LNGGAVRR451 pKa = 11.84PLSIGPDDD459 pKa = 3.18RR460 pKa = 11.84RR461 pKa = 11.84LQEEE465 pKa = 4.19GDDD468 pKa = 3.84NVDDD472 pKa = 2.57AFAEEE477 pKa = 4.3DDD479 pKa = 3.38SAADDD484 pKa = 4.19DD485 pKa = 3.92VTLVATLANGTTVSRR500 pKa = 11.84DDD502 pKa = 3.13TVAYYY507 pKa = 9.96EE508 pKa = 5.11DD509 pKa = 4.61EEE511 pKa = 4.29DD512 pKa = 3.23PRR514 pKa = 11.84NYYY517 pKa = 10.66IDDD520 pKa = 3.59SEEE523 pKa = 3.91ADDD526 pKa = 4.12RR527 pKa = 11.84DDD529 pKa = 3.49VQVVDDD535 pKa = 4.16TWAIGPDDD543 pKa = 3.49VRR545 pKa = 11.84PVDDD549 pKa = 3.91GYYY552 pKa = 11.13DD553 pKa = 3.44LLVVGDDD560 pKa = 4.59GWDDD564 pKa = 3.32YYY566 pKa = 11.01LEEE569 pKa = 4.64TVTTHHH575 pKa = 7.23DD576 pKa = 4.72EEE578 pKa = 4.85VDDD581 pKa = 3.61LGRR584 pKa = 11.84DDD586 pKa = 3.08GAFAIGMLWGGHHH599 pKa = 5.48DDD601 pKa = 4.97DD602 pKa = 6.4IPDDD606 pKa = 4.27QPKKK610 pKa = 9.64GWHHH614 pKa = 6.12GAAFFYYY621 pKa = 8.76EEE623 pKa = 3.72LEEE626 pKa = 3.85HHH628 pKa = 6.63YYY630 pKa = 11.26HH631 pKa = 7.65DD632 pKa = 3.54FEEE635 pKa = 4.49YY636 pKa = 10.72GVNNNVSLEEE646 pKa = 3.85EE647 pKa = 4.34DD648 pKa = 2.91KK649 pKa = 10.89YYY651 pKa = 11.18IIVEEE656 pKa = 3.93HHH658 pKa = 4.77VGIYYY663 pKa = 9.48DD664 pKa = 3.29RR665 pKa = 11.84YYY667 pKa = 10.54MKKK670 pKa = 9.29WEEE673 pKa = 4.69EE674 pKa = 4.25TPEEE678 pKa = 3.72TAWSLDDD685 pKa = 3.83IEEE688 pKa = 4.63FSLDDD693 pKa = 3.47EE694 pKa = 4.47PATGGIYYY702 pKa = 10.68NAHHH706 pKa = 6.2YY707 pKa = 10.57YY708 pKa = 10.34DD709 pKa = 3.82SFGDDD714 pKa = 4.04LVSEEE719 pKa = 4.58TGDDD723 pKa = 4.19DD724 pKa = 3.27VSGSDDD730 pKa = 3.13EE731 pKa = 4.83EEE733 pKa = 3.9LIAVTASDDD742 pKa = 3.79TSGLGEEE749 pKa = 3.67DDD751 pKa = 3.74FTGGGGSDDD760 pKa = 3.4FVLGDDD766 pKa = 3.85SRR768 pKa = 11.84AYYY771 pKa = 10.97YY772 pKa = 10.47DD773 pKa = 4.67DD774 pKa = 4.62LAATAGMADDD784 pKa = 3.82YY785 pKa = 11.43FVWDDD790 pKa = 5.2DDD792 pKa = 3.76TEEE795 pKa = 4.94DD796 pKa = 3.73VQLHHH801 pKa = 6.49SAADDD806 pKa = 3.63YY807 pKa = 11.36LTEEE811 pKa = 4.92DD812 pKa = 4.82SGLPPGTAIWRR823 pKa = 11.84LGGAEEE829 pKa = 4.14EEE831 pKa = 4.5VGHHH835 pKa = 6.09HHH837 pKa = 6.29EE838 pKa = 5.62YYY840 pKa = 11.03LSLTAGMFSFTDDD853 pKa = 3.53DD854 pKa = 4.93IA

Molecular weight: 90.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I2C229|A0A1I2C229_9RHOB DNA-binding transcriptional regulator IclR family OS=Roseivivax sediminis OX=936889 GN=SAMN04515678_11288 PE=4 SV=1
MM1 pKa = 7.84SMLKK5 pKa = 10.42CKK7 pKa = 10.16MPGAALRR14 pKa = 11.84VRR16 pKa = 11.84ASAAALTLSIIAVGASAGAQTLTEE40 pKa = 4.39FTPKK44 pKa = 10.33LPEE47 pKa = 4.07VAVDD51 pKa = 3.62TSPQAVEE58 pKa = 4.02VSLRR62 pKa = 11.84PQMRR66 pKa = 11.84DD67 pKa = 2.79DD68 pKa = 4.46RR69 pKa = 11.84LPKK72 pKa = 10.23ARR74 pKa = 11.84WGDD77 pKa = 3.84SVRR80 pKa = 11.84GVQWTRR86 pKa = 11.84AVVTALRR93 pKa = 11.84GHH95 pKa = 7.19AEE97 pKa = 4.09PLVDD101 pKa = 3.98TVPRR105 pKa = 11.84DD106 pKa = 3.36IDD108 pKa = 3.76DD109 pKa = 3.4WCPAYY114 pKa = 10.33RR115 pKa = 11.84QNNDD119 pKa = 3.13RR120 pKa = 11.84LRR122 pKa = 11.84EE123 pKa = 4.14AFWVGLVSSLAKK135 pKa = 10.39HH136 pKa = 5.73EE137 pKa = 4.67STWRR141 pKa = 11.84PTVVGGGGRR150 pKa = 11.84WHH152 pKa = 7.22GLLQILPSTARR163 pKa = 11.84LYY165 pKa = 11.0GCRR168 pKa = 11.84AQSGSALLSGPPNLSCGLRR187 pKa = 11.84IMAKK191 pKa = 6.56TVRR194 pKa = 11.84RR195 pKa = 11.84DD196 pKa = 3.6GVVSAGMRR204 pKa = 11.84GVAADD209 pKa = 3.73WGPFHH214 pKa = 7.25SRR216 pKa = 11.84TKK218 pKa = 10.11RR219 pKa = 11.84ADD221 pKa = 2.98MMAYY225 pKa = 8.48TRR227 pKa = 11.84NQAFCKK233 pKa = 9.59VVPGTRR239 pKa = 11.84PEE241 pKa = 3.96LRR243 pKa = 11.84PDD245 pKa = 2.8RR246 pKa = 11.84WEE248 pKa = 4.18APVMARR254 pKa = 11.84APHH257 pKa = 6.42HH258 pKa = 6.51PAPDD262 pKa = 3.91GPVLSTQGRR271 pKa = 11.84YY272 pKa = 7.24YY273 pKa = 11.23ARR275 pKa = 4.59

Molecular weight: 29.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4371

0

4371

1359608

24

5077

311.1

33.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.043 ± 0.062

0.867 ± 0.013

6.257 ± 0.046

6.319 ± 0.039

3.544 ± 0.025

9.109 ± 0.049

2.044 ± 0.017

4.705 ± 0.029

2.445 ± 0.031

9.966 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.581 ± 0.02

2.224 ± 0.022

5.291 ± 0.028

2.806 ± 0.024

7.449 ± 0.044

4.974 ± 0.026

5.484 ± 0.03

7.368 ± 0.033

1.405 ± 0.018

2.117 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski