Providencia sneebia DSM 19967
Average proteome isoelectric point is 6.37
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3113 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|K8WEP6|K8WEP6_9GAMM Arginine transporter ATP-binding subunit OS=Providencia sneebia DSM 19967 OX=1141660 GN=artP PE=3 SV=1
MM1 pKa = 7.81 KK2 pKa = 10.19 IGLFYY7 pKa = 10.96 GSSTCYY13 pKa = 10.19 TEE15 pKa = 4.84 IVAEE19 pKa = 4.56 KK20 pKa = 10.32 IRR22 pKa = 11.84 DD23 pKa = 3.51 ILGDD27 pKa = 3.76 GFVTLHH33 pKa = 6.07 NVNDD37 pKa = 4.25 TPPEE41 pKa = 3.75 MMEE44 pKa = 4.09 QYY46 pKa = 10.94 NILILGIPTWDD57 pKa = 3.26 FGEE60 pKa = 4.27 IQEE63 pKa = 4.57 DD64 pKa = 3.8 WLTIWDD70 pKa = 4.52 ALPNLQLQDD79 pKa = 4.61 KK80 pKa = 8.94 IIAMYY85 pKa = 11.01 GMGDD89 pKa = 3.51 QIDD92 pKa = 3.87 YY93 pKa = 11.36 SEE95 pKa = 4.5 WFLDD99 pKa = 3.63 ALGMLYY105 pKa = 10.13 HH106 pKa = 5.89 QLKK109 pKa = 8.04 PTGAKK114 pKa = 9.68 FVGFWPLDD122 pKa = 3.54 GYY124 pKa = 11.31 EE125 pKa = 4.2 FDD127 pKa = 3.97 SPKK130 pKa = 10.56 PLTEE134 pKa = 5.52 DD135 pKa = 3.47 GSQFVGLALDD145 pKa = 4.41 DD146 pKa = 4.14 VNQFEE151 pKa = 4.5 QTDD154 pKa = 3.47 EE155 pKa = 6.17 RR156 pKa = 11.84 IAQWCVQILQEE167 pKa = 4.83 IEE169 pKa = 3.96 EE170 pKa = 4.38 LLL172 pKa = 4.04
Molecular weight: 19.78 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.743
IPC2_protein 3.821
IPC_protein 3.795
Toseland 3.592
ProMoST 3.961
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.706
Rodwell 3.63
Grimsley 3.503
Solomon 3.77
Lehninger 3.732
Nozaki 3.897
DTASelect 4.101
Thurlkill 3.643
EMBOSS 3.719
Sillero 3.923
Patrickios 1.875
IPC_peptide 3.77
IPC2_peptide 3.897
IPC2.peptide.svr19 3.816
Protein with the highest isoelectric point:
>tr|K8WLG1|K8WLG1_9GAMM tRNA modification GTPase MnmE OS=Providencia sneebia DSM 19967 OX=1141660 GN=mnmE PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.6 RR12 pKa = 11.84 NRR14 pKa = 11.84 SHH16 pKa = 7.16 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.4 NGRR28 pKa = 11.84 QVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.22 GRR39 pKa = 11.84 ARR41 pKa = 11.84 LTVSSKK47 pKa = 11.16
Molecular weight: 5.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3113
0
3113
951914
26
2847
305.8
34.12
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.111 ± 0.052
1.064 ± 0.015
5.219 ± 0.029
6.037 ± 0.047
4.104 ± 0.038
6.717 ± 0.045
2.163 ± 0.023
7.413 ± 0.046
5.531 ± 0.041
10.465 ± 0.052
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.637 ± 0.02
4.723 ± 0.044
4.1 ± 0.026
4.581 ± 0.046
4.571 ± 0.038
6.408 ± 0.034
5.25 ± 0.03
6.417 ± 0.039
1.259 ± 0.017
3.22 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here