Testudinid alphaherpesvirus 3
Average proteome isoelectric point is 6.54
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 100 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0K1R170|A0A0K1R170_9ALPH Envelope glycoprotein L OS=Testudinid alphaherpesvirus 3 OX=2560801 GN=ORF25 PE=4 SV=1
MM1 pKa = 7.69 LLTFRR6 pKa = 11.84 NPRR9 pKa = 11.84 AIRR12 pKa = 11.84 DD13 pKa = 3.55 IVSIGMFLRR22 pKa = 11.84 SVTICGLFLSGFVVCYY38 pKa = 8.67 PAQTHH43 pKa = 5.71 SPTYY47 pKa = 10.67 GGTLPTDD54 pKa = 3.25 VSDD57 pKa = 4.17 GLRR60 pKa = 11.84 STEE63 pKa = 3.76 KK64 pKa = 10.07 PRR66 pKa = 11.84 PAEE69 pKa = 3.74 EE70 pKa = 3.71 QKK72 pKa = 10.91 IIDD75 pKa = 4.23 TLNQKK80 pKa = 9.94 CVLQHH85 pKa = 6.75 DD86 pKa = 4.46 VEE88 pKa = 4.82 FKK90 pKa = 10.42 NCAIQVSFSDD100 pKa = 4.27 PEE102 pKa = 3.93 ITLALYY108 pKa = 10.52 DD109 pKa = 4.38 HH110 pKa = 7.12 LALYY114 pKa = 7.08 QQCVIQFTSFAEE126 pKa = 4.52 GTTTCEE132 pKa = 3.61 SWGPTPIPLDD142 pKa = 3.52 LCLCDD147 pKa = 4.13 EE148 pKa = 5.03 GGLNKK153 pKa = 10.33 CAVSGAKK160 pKa = 9.1 TIHH163 pKa = 5.57 VFYY166 pKa = 10.11 PSGPAVSAAKK176 pKa = 9.63 FQKK179 pKa = 10.49 NQTLLITTDD188 pKa = 3.17 DD189 pKa = 3.87 CGRR192 pKa = 11.84 TLSTASTASPISDD205 pKa = 3.81 SEE207 pKa = 4.2 KK208 pKa = 10.3 HH209 pKa = 6.57 RR210 pKa = 11.84 SICFQLSPKK219 pKa = 9.71 PEE221 pKa = 4.5 NIGQHH226 pKa = 4.82 ILRR229 pKa = 11.84 VIRR232 pKa = 11.84 QPLTAISRR240 pKa = 11.84 QGPFSYY246 pKa = 11.14 LNDD249 pKa = 3.34 THH251 pKa = 6.82 TLLLPGPVSQEE262 pKa = 3.52 QHH264 pKa = 5.6 DD265 pKa = 4.36 TYY267 pKa = 11.26 DD268 pKa = 3.42 GSGEE272 pKa = 4.04 MEE274 pKa = 4.6 PNWFNYY280 pKa = 8.29 TYY282 pKa = 10.94 QDD284 pKa = 4.06 DD285 pKa = 4.29 LTLALYY291 pKa = 10.68 SGDD294 pKa = 3.99 LLDD297 pKa = 4.8 VLGDD301 pKa = 3.52 DD302 pKa = 3.54 TVFFVFPDD310 pKa = 4.4 SICVNYY316 pKa = 9.68 TSHH319 pKa = 7.48 PYY321 pKa = 10.3 SEE323 pKa = 4.72 TSAEE327 pKa = 4.13 YY328 pKa = 10.73 GDD330 pKa = 3.82 SSEE333 pKa = 4.05 ALEE336 pKa = 4.1 YY337 pKa = 10.7 TIGRR341 pKa = 11.84 VDD343 pKa = 3.54 ACTLTAIQTQINGTVIYY360 pKa = 9.81 DD361 pKa = 3.62 VPCEE365 pKa = 3.9 EE366 pKa = 4.55 GQTCFGYY373 pKa = 10.87 VYY375 pKa = 10.6 GDD377 pKa = 3.4 RR378 pKa = 11.84 MKK380 pKa = 10.59 LTVAHH385 pKa = 6.93 PGYY388 pKa = 6.98 TTQEE392 pKa = 4.09 HH393 pKa = 6.71 NYY395 pKa = 9.31 GNYY398 pKa = 9.6 VEE400 pKa = 5.55 LDD402 pKa = 3.89 AYY404 pKa = 11.22 DD405 pKa = 4.3 MITPEE410 pKa = 4.52 PGSPSKK416 pKa = 10.81 HH417 pKa = 6.29 IIWKK421 pKa = 5.75 TTLFSDD427 pKa = 4.96 LSDD430 pKa = 4.35 DD431 pKa = 4.88 FVDD434 pKa = 3.62 TRR436 pKa = 11.84 LNRR439 pKa = 11.84 HH440 pKa = 5.45 QLDD443 pKa = 4.23 GVCDD447 pKa = 4.11 DD448 pKa = 4.77 EE449 pKa = 4.85 GCPGEE454 pKa = 4.99 GSWEE458 pKa = 4.24 DD459 pKa = 3.33 IYY461 pKa = 10.75 PTVHH465 pKa = 6.33 EE466 pKa = 4.45
Molecular weight: 51.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.318
IPC2_protein 4.444
IPC_protein 4.418
Toseland 4.253
ProMoST 4.533
Dawson 4.393
Bjellqvist 4.558
Wikipedia 4.291
Rodwell 4.266
Grimsley 4.164
Solomon 4.393
Lehninger 4.355
Nozaki 4.507
DTASelect 4.711
Thurlkill 4.266
EMBOSS 4.317
Sillero 4.546
Patrickios 0.629
IPC_peptide 4.393
IPC2_peptide 4.533
IPC2.peptide.svr19 4.44
Protein with the highest isoelectric point:
>tr|A0A0K1R159|A0A0K1R159_9ALPH ICP4-like protein OS=Testudinid alphaherpesvirus 3 OX=2560801 GN=ORF2 PE=4 SV=1
MM1 pKa = 7.66 EE2 pKa = 5.31 SGGNGRR8 pKa = 11.84 GRR10 pKa = 11.84 PRR12 pKa = 11.84 GRR14 pKa = 11.84 PTRR17 pKa = 11.84 RR18 pKa = 11.84 SRR20 pKa = 11.84 GRR22 pKa = 11.84 RR23 pKa = 11.84 GPSPPRR29 pKa = 11.84 TRR31 pKa = 11.84 SRR33 pKa = 11.84 SPLRR37 pKa = 11.84 HH38 pKa = 6.02 GPDD41 pKa = 3.26 SPEE44 pKa = 4.37 PGPSGSGMVPRR55 pKa = 11.84 LVRR58 pKa = 11.84 PSPGDD63 pKa = 3.36 PEE65 pKa = 4.16 QAHH68 pKa = 6.86 PGSPARR74 pKa = 11.84 QQPRR78 pKa = 11.84 DD79 pKa = 3.61 NGGSSDD85 pKa = 3.8 STVSLSNPEE94 pKa = 4.01 SDD96 pKa = 3.33 SGQITSSDD104 pKa = 3.75 SGDD107 pKa = 3.69 EE108 pKa = 3.9 AHH110 pKa = 7.37 SPGLVQGPVSPGPTPFPRR128 pKa = 11.84 PGTLPVEE135 pKa = 4.49 VGDD138 pKa = 4.07 VSSSTSVQAPPTGNQGRR155 pKa = 11.84 SPGEE159 pKa = 3.86 VAPDD163 pKa = 3.79 PPHH166 pKa = 6.11 IPRR169 pKa = 11.84 WVRR172 pKa = 11.84 LAGQPNIGPMPPIARR187 pKa = 11.84 DD188 pKa = 3.38 QRR190 pKa = 11.84 ISEE193 pKa = 4.34 DD194 pKa = 4.26 HH195 pKa = 6.92 PPADD199 pKa = 3.77 PGNYY203 pKa = 9.35 VFIIYY208 pKa = 9.17 TGG210 pKa = 3.16
Molecular weight: 21.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.281
IPC2_protein 9.194
IPC_protein 10.613
Toseland 10.248
ProMoST 10.613
Dawson 10.452
Bjellqvist 10.409
Wikipedia 10.877
Rodwell 10.131
Grimsley 10.555
Solomon 10.774
Lehninger 10.687
Nozaki 10.233
DTASelect 10.409
Thurlkill 10.335
EMBOSS 10.73
Sillero 10.409
Patrickios 9.838
IPC_peptide 10.76
IPC2_peptide 9.765
IPC2.peptide.svr19 8.605
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
100
0
100
42401
79
2574
424.0
48.03
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.021 ± 0.201
2.323 ± 0.166
5.46 ± 0.14
5.604 ± 0.154
4.328 ± 0.151
5.243 ± 0.176
2.535 ± 0.092
6.141 ± 0.17
4.627 ± 0.173
9.698 ± 0.185
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.571 ± 0.097
4.795 ± 0.136
5.354 ± 0.225
3.266 ± 0.116
5.887 ± 0.171
7.493 ± 0.176
6.762 ± 0.143
6.415 ± 0.129
1.149 ± 0.087
4.33 ± 0.148
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here