Streptococcus infantis SK1302
Average proteome isoelectric point is 6.24
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2102 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E1M9D8|E1M9D8_9STRE Aminoglycoside 6-adenylyltransferase OS=Streptococcus infantis SK1302 OX=871237 GN=SIN_1529 PE=4 SV=1
MM1 pKa = 7.95 RR2 pKa = 11.84 KK3 pKa = 8.89 YY4 pKa = 10.56 QEE6 pKa = 4.57 LSLEE10 pKa = 4.17 QIIEE14 pKa = 4.12 QVRR17 pKa = 11.84 ADD19 pKa = 4.04 QLTSDD24 pKa = 4.72 DD25 pKa = 3.49 FCLYY29 pKa = 10.55 GKK31 pKa = 10.37 EE32 pKa = 4.05 DD33 pKa = 3.84 GEE35 pKa = 4.42 LALDD39 pKa = 3.64 RR40 pKa = 11.84 LYY42 pKa = 10.88 WMSDD46 pKa = 3.38 YY47 pKa = 11.06 PDD49 pKa = 3.45 VVEE52 pKa = 6.21 DD53 pKa = 3.9 RR54 pKa = 11.84 DD55 pKa = 3.76 IYY57 pKa = 10.27 PADD60 pKa = 3.66 VVEE63 pKa = 4.95 QDD65 pKa = 3.74 LQLVYY70 pKa = 10.87 YY71 pKa = 9.89 GEE73 pKa = 3.98 QLIDD77 pKa = 3.63 VLTVALEE84 pKa = 4.21 EE85 pKa = 4.79 KK86 pKa = 10.3 PDD88 pKa = 3.98 ASPQDD93 pKa = 3.49 LVEE96 pKa = 4.06 ALKK99 pKa = 10.84 YY100 pKa = 8.84 YY101 pKa = 8.9 QQHH104 pKa = 6.83 DD105 pKa = 3.62 SFMPFEE111 pKa = 4.22 PP112 pKa = 4.57
Molecular weight: 13.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.711
IPC2_protein 3.808
IPC_protein 3.783
Toseland 3.579
ProMoST 3.948
Dawson 3.77
Bjellqvist 3.935
Wikipedia 3.706
Rodwell 3.617
Grimsley 3.49
Solomon 3.77
Lehninger 3.719
Nozaki 3.897
DTASelect 4.101
Thurlkill 3.63
EMBOSS 3.719
Sillero 3.91
Patrickios 0.998
IPC_peptide 3.757
IPC2_peptide 3.884
IPC2.peptide.svr19 3.817
Protein with the highest isoelectric point:
>tr|E1M6U0|E1M6U0_9STRE Regulator of sorbitol operon OS=Streptococcus infantis SK1302 OX=871237 GN=SIN_0641 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.24 QPSKK9 pKa = 9.82 LRR11 pKa = 11.84 RR12 pKa = 11.84 ARR14 pKa = 11.84 KK15 pKa = 8.57 HH16 pKa = 4.75 GFRR19 pKa = 11.84 NRR21 pKa = 11.84 MSTKK25 pKa = 9.22 NGRR28 pKa = 11.84 RR29 pKa = 11.84 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 8.87 GRR39 pKa = 11.84 KK40 pKa = 8.75 VLAAA44 pKa = 4.31
Molecular weight: 5.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.449
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.398
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.135
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.082
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2102
0
2102
473331
37
2363
225.2
25.3
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.399 ± 0.073
0.612 ± 0.018
5.622 ± 0.053
7.374 ± 0.066
4.654 ± 0.055
6.539 ± 0.052
1.852 ± 0.027
7.142 ± 0.065
6.836 ± 0.05
10.1 ± 0.078
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.556 ± 0.031
4.372 ± 0.043
3.356 ± 0.035
4.043 ± 0.035
4.06 ± 0.044
6.192 ± 0.051
5.567 ± 0.076
7.016 ± 0.05
0.935 ± 0.02
3.774 ± 0.047
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here