Aspergillus rambellii

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; Eurotiomycetes; Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7761 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0F8XCL8|A0A0F8XCL8_9EURO Uncharacterized protein OS=Aspergillus rambellii OX=308745 GN=ARAM_002239 PE=4 SV=1
MM1 pKa = 7.68SILKK5 pKa = 10.66LSDD8 pKa = 3.23NQSLKK13 pKa = 11.19DD14 pKa = 3.53KK15 pKa = 9.65LTAIEE20 pKa = 3.97VSYY23 pKa = 10.61SAKK26 pKa = 9.65LQSWTSEE33 pKa = 3.99TIVNNTRR40 pKa = 11.84TVLKK44 pKa = 9.65ATLWNIAQKK53 pKa = 9.9SLCEE57 pKa = 3.92TEE59 pKa = 4.61RR60 pKa = 11.84PQKK63 pKa = 10.3ISPLYY68 pKa = 8.8STEE71 pKa = 3.79NQIPNDD77 pKa = 3.41ILFSEE82 pKa = 5.05ACTEE86 pKa = 4.23DD87 pKa = 4.42LFDD90 pKa = 4.88INDD93 pKa = 3.38YY94 pKa = 7.5TTIYY98 pKa = 10.26SDD100 pKa = 3.35SDD102 pKa = 3.86PYY104 pKa = 11.33LGTEE108 pKa = 4.82DD109 pKa = 4.09GYY111 pKa = 11.5EE112 pKa = 3.93YY113 pKa = 11.06DD114 pKa = 4.08DD115 pKa = 4.07TEE117 pKa = 4.43GAASILFSGDD127 pKa = 3.21NLSEE131 pKa = 4.13TSAEE135 pKa = 4.19RR136 pKa = 11.84LPGDD140 pKa = 3.61QTDD143 pKa = 4.92LDD145 pKa = 4.12PEE147 pKa = 4.61IYY149 pKa = 7.61TTTTSEE155 pKa = 4.41TINGSSWKK163 pKa = 8.6MQYY166 pKa = 9.59TPISKK171 pKa = 10.01HH172 pKa = 4.87SGYY175 pKa = 10.67VEE177 pKa = 4.76TYY179 pKa = 10.33QEE181 pKa = 4.47DD182 pKa = 3.73SGLLIDD188 pKa = 6.22DD189 pKa = 4.1VFEE192 pKa = 5.78DD193 pKa = 4.07SLSGQTPHH201 pKa = 6.08SHH203 pKa = 6.6PLEE206 pKa = 4.86FYY208 pKa = 5.79TTKK211 pKa = 10.82YY212 pKa = 9.63PDD214 pKa = 3.5NEE216 pKa = 4.28EE217 pKa = 3.93TDD219 pKa = 4.55LMLSDD224 pKa = 4.96DD225 pKa = 3.89

Molecular weight: 25.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0F8UNP1|A0A0F8UNP1_9EURO Uncharacterized protein OS=Aspergillus rambellii OX=308745 GN=ARAM_005135 PE=3 SV=1
MM1 pKa = 7.39LCVRR5 pKa = 11.84CRR7 pKa = 11.84AIPSALRR14 pKa = 11.84TATSLTTRR22 pKa = 11.84QLIAPVSPLSSLPSQLRR39 pKa = 11.84SFSLATRR46 pKa = 11.84PSIHH50 pKa = 6.62PQPHH54 pKa = 5.86LQSSLSSIRR63 pKa = 11.84SHH65 pKa = 6.12IQSTLSASPSPAQQTRR81 pKa = 11.84SFSASSSLAGKK92 pKa = 8.2RR93 pKa = 11.84TTYY96 pKa = 10.38NPSRR100 pKa = 11.84RR101 pKa = 11.84VQKK104 pKa = 10.13RR105 pKa = 11.84RR106 pKa = 11.84HH107 pKa = 5.28GFLTRR112 pKa = 11.84LRR114 pKa = 11.84SKK116 pKa = 10.71GGRR119 pKa = 11.84KK120 pKa = 8.84ILLRR124 pKa = 11.84RR125 pKa = 11.84KK126 pKa = 9.56AKK128 pKa = 9.75GRR130 pKa = 11.84KK131 pKa = 8.26SLSWW135 pKa = 3.4

Molecular weight: 14.93 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7761

0

7761

4081960

8

6276

526.0

58.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.366 ± 0.021

1.187 ± 0.01

5.625 ± 0.019

6.243 ± 0.032

3.735 ± 0.015

6.669 ± 0.021

2.418 ± 0.011

4.955 ± 0.014

4.625 ± 0.022

9.107 ± 0.028

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.088 ± 0.009

3.643 ± 0.015

6.238 ± 0.029

4.12 ± 0.019

6.23 ± 0.025

8.616 ± 0.032

5.933 ± 0.017

6.056 ± 0.02

1.38 ± 0.009

2.767 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski