Terasakiispira papahanaumokuakeensis
Average proteome isoelectric point is 6.11
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3403 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1E2V732|A0A1E2V732_9GAMM LysR family transcriptional regulator OS=Terasakiispira papahanaumokuakeensis OX=197479 GN=BFW38_03900 PE=3 SV=1
MM1 pKa = 7.66 AISASFEE8 pKa = 4.07 EE9 pKa = 4.64 KK10 pKa = 10.73 VYY12 pKa = 11.06 LLAYY16 pKa = 7.31 TAYY19 pKa = 10.24 GKK21 pKa = 10.24 DD22 pKa = 3.5 LTDD25 pKa = 3.37 MQGDD29 pKa = 4.47 FLTSYY34 pKa = 9.82 GQNDD38 pKa = 3.85 LHH40 pKa = 7.74 KK41 pKa = 10.32 VAQFLLDD48 pKa = 3.56 SGYY51 pKa = 11.08 LGTDD55 pKa = 3.23 YY56 pKa = 11.39 SGTTDD61 pKa = 3.27 RR62 pKa = 11.84 QFVSSLTEE70 pKa = 3.96 SYY72 pKa = 11.9 ANGLVSAEE80 pKa = 4.0 RR81 pKa = 11.84 QQVTKK86 pKa = 10.65 QYY88 pKa = 8.07 FTNEE92 pKa = 3.79 LQQDD96 pKa = 4.19 GVDD99 pKa = 3.59 KK100 pKa = 11.26 ADD102 pKa = 3.17 IMVNMLEE109 pKa = 3.97 AMLRR113 pKa = 11.84 STDD116 pKa = 4.2 SEE118 pKa = 4.24 WSTAASNAYY127 pKa = 9.04 TEE129 pKa = 4.62 VLSNMNDD136 pKa = 3.18 FSSFAFEE143 pKa = 4.19 ATPSDD148 pKa = 4.01 EE149 pKa = 4.29 LTGVDD154 pKa = 4.56 AFDD157 pKa = 3.99 FTDD160 pKa = 3.11 HH161 pKa = 7.16 HH162 pKa = 7.55 DD163 pKa = 3.87 DD164 pKa = 3.63 AVVYY168 pKa = 10.13 SQAPVDD174 pKa = 3.66 SHH176 pKa = 7.62 DD177 pKa = 3.4 ITLVGTADD185 pKa = 3.58 VPALEE190 pKa = 4.46 TDD192 pKa = 3.47 SVLII196 pKa = 4.35
Molecular weight: 21.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.691
IPC2_protein 3.783
IPC_protein 3.783
Toseland 3.567
ProMoST 3.961
Dawson 3.783
Bjellqvist 3.948
Wikipedia 3.732
Rodwell 3.617
Grimsley 3.478
Solomon 3.783
Lehninger 3.732
Nozaki 3.897
DTASelect 4.164
Thurlkill 3.63
EMBOSS 3.745
Sillero 3.91
Patrickios 1.1
IPC_peptide 3.77
IPC2_peptide 3.884
IPC2.peptide.svr19 3.799
Protein with the highest isoelectric point:
>tr|A0A1E2VCV0|A0A1E2VCV0_9GAMM Probable chromosome-partitioning protein ParB OS=Terasakiispira papahanaumokuakeensis OX=197479 GN=BFW38_15860 PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.49 RR12 pKa = 11.84 KK13 pKa = 7.97 RR14 pKa = 11.84 THH16 pKa = 5.89 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.37 NGRR28 pKa = 11.84 KK29 pKa = 8.96 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.19 GRR39 pKa = 11.84 HH40 pKa = 5.26 RR41 pKa = 11.84 LSAA44 pKa = 3.8
Molecular weight: 5.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.257
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.705
Grimsley 13.042
Solomon 13.51
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.427
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.173
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3403
0
3403
1145210
39
5688
336.5
37.2
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.135 ± 0.05
0.961 ± 0.013
5.621 ± 0.039
5.798 ± 0.041
3.437 ± 0.026
7.369 ± 0.04
2.749 ± 0.024
5.172 ± 0.037
3.131 ± 0.033
11.414 ± 0.063
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.693 ± 0.022
2.924 ± 0.033
4.826 ± 0.031
5.643 ± 0.057
6.023 ± 0.041
5.926 ± 0.038
5.29 ± 0.034
6.746 ± 0.037
1.567 ± 0.022
2.577 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here