Stenotrophomonas phage IME-SM1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Menderavirus; Stenotrophomonas virus IMESM1

Average proteome isoelectric point is 6.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 202 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0H4INM7|A0A0H4INM7_9CAUD Uncharacterized protein OS=Stenotrophomonas phage IME-SM1 OX=1654717 PE=4 SV=1
MM1 pKa = 7.58KK2 pKa = 10.2IIAKK6 pKa = 9.73DD7 pKa = 3.3QAQADD12 pKa = 4.26TLVKK16 pKa = 10.71AFNLSAADD24 pKa = 3.95LEE26 pKa = 4.55NTLVQNGLEE35 pKa = 4.14IEE37 pKa = 4.9LDD39 pKa = 4.02DD40 pKa = 5.57LDD42 pKa = 5.85LDD44 pKa = 3.91IMIDD48 pKa = 3.39GLYY51 pKa = 8.48YY52 pKa = 10.42AGYY55 pKa = 8.57ASEE58 pKa = 4.4VIEE61 pKa = 4.4VLLDD65 pKa = 3.42ALGSYY70 pKa = 9.85DD71 pKa = 3.72HH72 pKa = 7.15NSILVLL78 pKa = 3.88

Molecular weight: 8.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0H4IP50|A0A0H4IP50_9CAUD Uncharacterized protein OS=Stenotrophomonas phage IME-SM1 OX=1654717 PE=4 SV=1
MM1 pKa = 7.31VSLIYY6 pKa = 10.29TLASTYY12 pKa = 10.34KK13 pKa = 9.45PKK15 pKa = 10.68KK16 pKa = 8.1KK17 pKa = 9.77RR18 pKa = 11.84KK19 pKa = 8.21PKK21 pKa = 10.68GEE23 pKa = 3.92VFQKK27 pKa = 10.52YY28 pKa = 9.49KK29 pKa = 9.69PDD31 pKa = 3.53KK32 pKa = 10.37SFKK35 pKa = 10.31PLTTTPSRR43 pKa = 11.84PIRR46 pKa = 11.84QSDD49 pKa = 3.89QYY51 pKa = 11.4KK52 pKa = 9.55SAAMPAAKK60 pKa = 9.39PVLPVRR66 pKa = 11.84YY67 pKa = 8.62EE68 pKa = 3.98GEE70 pKa = 3.91MAEE73 pKa = 4.61RR74 pKa = 11.84EE75 pKa = 3.98AAAQRR80 pKa = 11.84EE81 pKa = 4.51IEE83 pKa = 4.28RR84 pKa = 11.84KK85 pKa = 9.42RR86 pKa = 11.84RR87 pKa = 11.84WSLRR91 pKa = 11.84CTTRR95 pKa = 11.84AATNMWVMFRR105 pKa = 11.84PKK107 pKa = 10.38SS108 pKa = 3.47

Molecular weight: 12.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

202

0

202

41889

38

1328

207.4

23.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.699 ± 0.192

1.027 ± 0.066

6.641 ± 0.12

6.532 ± 0.227

4.218 ± 0.134

6.639 ± 0.18

1.728 ± 0.103

5.956 ± 0.13

6.116 ± 0.219

7.969 ± 0.167

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.76 ± 0.099

4.755 ± 0.129

4.068 ± 0.124

4.015 ± 0.119

5.262 ± 0.137

6.099 ± 0.185

6.212 ± 0.24

6.756 ± 0.136

1.662 ± 0.073

3.886 ± 0.132
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski